Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 121998216

GI number: 121998216

Start: 1546523

End: 1547014

Strand: Reverse

Name: ispF

Synonym: Hhal_1434

Alternate gene names: 121998216

Gene position: 1547014-1546523 (Counterclockwise)

Preceding gene: 121998217

Following gene: 121998215

Centisome position: 57.76

GC content: 69.72

Gene sequence:

>492_bases
GTGATTCGCATTGGGCAGGGTTTCGACGTCCACCGGTTCGCCGGCAAGGGCGAGGGTTTTCGGCTGGGCGGGGTCGCGGT
CCCGTACCACAAGCGGTTGGAGGCCCACTCCGACGGCGATGTCCTCCTGCACGCGATCACCGACGGCCTGCTCGGCGCCT
GTGGGATGGGGGATATCGGTCGCCACTTCCCGGACGACGACCCGCGCTGGCGGGACGCCGACAGCATCGGTCTGCTGGCC
TCGACGCTGCGTTTGGCTGCCGAGGCCGGATGGGTGCCGGTGAATGTCGACGGCACGGTGATTGCTCAGGCCCCTAAGCT
GGCTCCCTATATCGGTGCCATGCGCCAGGCCACCGCCGACGCCATGGAGCTGTCGCCCGGGGCGGTGAACGTCAAGGCGA
CGACCAGCGAGCGCCTCGGCTTCACCGGCCGGGGAGAGGGGATCGCCGCGATGGCAGTGGTCCTGGTGGAATCGGCCCCG
GGGCGGGGTTGA

Upstream 100 bases:

>100_bases
GCGAAGCCGGCAATCTGAAGATCACCCATCAGGCTGACCTGGATCTGGCTGCTGCCGTGCTTACGGCGCAGCGGGCGGCC
ACGGAGAGGGAGCAAACCGC

Downstream 100 bases:

>100_bases
TCCGGGCCCACGGCGGACCCTTGGGCACGGCGCGCCTGCGCGCCTGCCCCGAGGACTTCCGCGTGGATGAGCAGCTGCCG
TTTCAGCCCTCCGGGGTGGG

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MIRIGQGFDVHRFAGKGEGFRLGGVAVPYHKRLEAHSDGDVLLHAITDGLLGACGMGDIGRHFPDDDPRWRDADSIGLLA
STLRLAAEAGWVPVNVDGTVIAQAPKLAPYIGAMRQATADAMELSPGAVNVKATTSERLGFTGRGEGIAAMAVVLVESAP
GRG

Sequences:

>Translated_163_residues
MIRIGQGFDVHRFAGKGEGFRLGGVAVPYHKRLEAHSDGDVLLHAITDGLLGACGMGDIGRHFPDDDPRWRDADSIGLLA
STLRLAAEAGWVPVNVDGTVIAQAPKLAPYIGAMRQATADAMELSPGAVNVKATTSERLGFTGRGEGIAAMAVVLVESAP
GRG
>Mature_163_residues
MIRIGQGFDVHRFAGKGEGFRLGGVAVPYHKRLEAHSDGDVLLHAITDGLLGACGMGDIGRHFPDDDPRWRDADSIGLLA
STLRLAAEAGWVPVNVDGTVIAQAPKLAPYIGAMRQATADAMELSPGAVNVKATTSERLGFTGRGEGIAAMAVVLVESAP
GRG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=55.6962025316456, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_HALHL (A1WWY9)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003003.1
- ProteinModelPortal:   A1WWY9
- SMR:   A1WWY9
- STRING:   A1WWY9
- GeneID:   4710721
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1434
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   A1WWY9
- BioCyc:   HHAL349124:HHAL_1434-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16972; Mature: 16972

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIGQGFDVHRFAGKGEGFRLGGVAVPYHKRLEAHSDGDVLLHAITDGLLGACGMGDIG
CEEECCCCCCCEECCCCCCEEECCEECCHHHHHCCCCCCCEEHEEHHHHHHCCCCCCCHH
RHFPDDDPRWRDADSIGLLASTLRLAAEAGWVPVNVDGTVIAQAPKLAPYIGAMRQATAD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHHHHH
AMELSPGAVNVKATTSERLGFTGRGEGIAAMAVVLVESAPGRG
HHCCCCCEEEEEECCHHHCCCCCCCCCHHHEEEEHEECCCCCC
>Mature Secondary Structure
MIRIGQGFDVHRFAGKGEGFRLGGVAVPYHKRLEAHSDGDVLLHAITDGLLGACGMGDIG
CEEECCCCCCCEECCCCCCEEECCEECCHHHHHCCCCCCCEEHEEHHHHHHCCCCCCCHH
RHFPDDDPRWRDADSIGLLASTLRLAAEAGWVPVNVDGTVIAQAPKLAPYIGAMRQATAD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCCHHHHHHHHHHHH
AMELSPGAVNVKATTSERLGFTGRGEGIAAMAVVLVESAPGRG
HHCCCCCEEEEEECCHHHCCCCCCCCCHHHEEEEHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA