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The map label for this gene is cbiF [H]

Identifier: 121998133

GI number: 121998133

Start: 1460203

End: 1460982

Strand: Reverse

Name: cbiF [H]

Synonym: Hhal_1351

Alternate gene names: 121998133

Gene position: 1460982-1460203 (Counterclockwise)

Preceding gene: 121998134

Following gene: 121998132

Centisome position: 54.55

GC content: 72.18

Gene sequence:

>780_bases
ATGAAGGGGGAGGTCTGGTTTGTGGGGGCCGGTCCCGGGGCGGCCGATCTAATCACGGTTCGCGGGCGGGATCGGGTGGC
TGCGGCGGACGCCGTGCTCTACGCCGGTTCGCTGGTGCCGGCCGAGGTGCTCGACTGGGCGCCGGCGCACTGCGAGGTCG
CCGACTCCAAAGGGATGACCTTGGAACAGATCCAGGACTGGCTGCTCGAGCGCGCCCGCGCCGGGGGCTGTGTGGTCCGC
CTTCAACCCGGTGATCCGGGGCTATACGGGGCCTTCGTGGAGATCGTGCGCCCGCTGGACGCCGCGGGGATCCCAGTCCA
TCTGGTTCCCGGGGTCACGTCGGCCATGGCCGCGGCGGCGGCGGCCGGCGAGAGCCTGACCCTGCCGGAGGGCACGCAGA
CGGTGATCTTCACCCGGGTGGAGGGGCGCACCCCGATGCCGGCGGGGGAACAGCTTGCCGATCTGGCGCGGCACGGCAGC
ACCCTGTGTGTCTACCTGTCGATCACCCTCCTGGAACGCCTGGCCGATGCCCTGCGCGGTGGCGGCTGGGACGACGACGC
CCCGGTGGTGGTGGTCCACCGCGCCTCCTGGCCCGGGGTCGAACAGGTCCTGCGTACCTGCATCCGCGACCTGGCCGCGG
ACTGCCGCGCGGCGGGGCTCAACAGCCAGACCATGATCCTGGTCGGTCCGACGCTCGGGGCGCGGGAGGCGGCACCGGCG
GCGCGCTCACGGCTTTACGACCCCGGTTTCGGCCACCGTTTCCGCCGCCGCGAAAATTGA

Upstream 100 bases:

>100_bases
CGCCGACTCTGCGTGCCGCCTACGACGTGGACGTTCACGTCGTCGAGCATCAGCGGGCACCGTTTGTGGCCGGTTACCAG
TTGCAAGAGAGGGGACGCGG

Downstream 100 bases:

>100_bases
GGAACGACATGCATGACTGACTGTATCCTGTTGATTGGGCATGGCTCGCGCCAGGAGGACGGCAACGAGGAGATCCGCCG
GTTCGCCGACCTGCTGGCCG

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKGEVWFVGAGPGAADLITVRGRDRVAAADAVLYAGSLVPAEVLDWAPAHCEVADSKGMTLEQIQDWLLERARAGGCVVR
LQPGDPGLYGAFVEIVRPLDAAGIPVHLVPGVTSAMAAAAAAGESLTLPEGTQTVIFTRVEGRTPMPAGEQLADLARHGS
TLCVYLSITLLERLADALRGGGWDDDAPVVVVHRASWPGVEQVLRTCIRDLAADCRAAGLNSQTMILVGPTLGAREAAPA
ARSRLYDPGFGHRFRRREN

Sequences:

>Translated_259_residues
MKGEVWFVGAGPGAADLITVRGRDRVAAADAVLYAGSLVPAEVLDWAPAHCEVADSKGMTLEQIQDWLLERARAGGCVVR
LQPGDPGLYGAFVEIVRPLDAAGIPVHLVPGVTSAMAAAAAAGESLTLPEGTQTVIFTRVEGRTPMPAGEQLADLARHGS
TLCVYLSITLLERLADALRGGGWDDDAPVVVVHRASWPGVEQVLRTCIRDLAADCRAAGLNSQTMILVGPTLGAREAAPA
ARSRLYDPGFGHRFRRREN
>Mature_259_residues
MKGEVWFVGAGPGAADLITVRGRDRVAAADAVLYAGSLVPAEVLDWAPAHCEVADSKGMTLEQIQDWLLERARAGGCVVR
LQPGDPGLYGAFVEIVRPLDAAGIPVHLVPGVTSAMAAAAAAGESLTLPEGTQTVIFTRVEGRTPMPAGEQLADLARHGS
TLCVYLSITLLERLADALRGGGWDDDAPVVVVHRASWPGVEQVLRTCIRDLAADCRAAGLNSQTMILVGPTLGAREAAPA
ARSRLYDPGFGHRFRRREN

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=248, Percent_Identity=29.4354838709677, Blast_Score=87, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6322922, Length=261, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27350; Mature: 27350

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEVWFVGAGPGAADLITVRGRDRVAAADAVLYAGSLVPAEVLDWAPAHCEVADSKGMT
CCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHCCCCCCEEECCCCCCC
LEQIQDWLLERARAGGCVVRLQPGDPGLYGAFVEIVRPLDAAGIPVHLVPGVTSAMAAAA
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHH
AAGESLTLPEGTQTVIFTRVEGRTPMPAGEQLADLARHGSTLCVYLSITLLERLADALRG
HCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHC
GGWDDDAPVVVVHRASWPGVEQVLRTCIRDLAADCRAAGLNSQTMILVGPTLGAREAAPA
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCH
ARSRLYDPGFGHRFRRREN
HHHHCCCCCCCCCHHCCCC
>Mature Secondary Structure
MKGEVWFVGAGPGAADLITVRGRDRVAAADAVLYAGSLVPAEVLDWAPAHCEVADSKGMT
CCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHCCCCCCEEECCCCCCC
LEQIQDWLLERARAGGCVVRLQPGDPGLYGAFVEIVRPLDAAGIPVHLVPGVTSAMAAAA
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHH
AAGESLTLPEGTQTVIFTRVEGRTPMPAGEQLADLARHGSTLCVYLSITLLERLADALRG
HCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHC
GGWDDDAPVVVVHRASWPGVEQVLRTCIRDLAADCRAAGLNSQTMILVGPTLGAREAAPA
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCH
ARSRLYDPGFGHRFRRREN
HHHHCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]