Click here to switch to the map view.

The map label for this gene is hsdS [H]

Identifier: 121997944

GI number: 121997944

Start: 1256434

End: 1257723

Strand: Reverse

Name: hsdS [H]

Synonym: Hhal_1153

Alternate gene names: 121997944

Gene position: 1257723-1256434 (Counterclockwise)

Preceding gene: 121997945

Following gene: 121997943

Centisome position: 46.96

GC content: 55.35

Gene sequence:

>1290_bases
ATGAGTTTCCCGGCGTATCCCGAGTACAAGGATTCTGGGGTCGAGTGGCTGGGGGAGGTGCCGGAGCATTGGTCCGTCAG
TGCGCTGAAGCGTGTTGCGCGTCTCGAAAGTGGCGACGCGATAAGCAGCGATCACATCAGTGAAGAGGGGGAGTATGCCG
TTTACGGCGGGAATGGCATAAGGGGTTTTTCATCTGGATACACTCACGACGGTTTTTACCCTTTGATTGGGCGCCAAGGA
GCTCTTTGCGGTAACGTCAATTACGCGAAAGGAAGGTTCTGGGCATCTGAGCATGCGGTTGTTGTTTGGCCTGGAAGACA
AATTGACGGTTTTTGGCTCGGTGAGCTTCTTCGCTCAATGAATCTTAATCAATATGCGACATCGGCTGCGCAACCGGGTT
TGTCGGTTGAGACTATTGAAAATCTTTATGTTCCTGTTCCGCCGGATGAAGAGCAACAAAAGATAGCGGAGCTCCTCGAC
CACGAAACCGCCCGTATCGACGCCCTGATCGAGGAGCAGCAGCGCCTGATCGAGCTGCTCAAGGAGAAGCGCCAGGCGGT
GATCTCCCATGCCGTCACCAAAGGCCTCGACCCCGATGTGCCGATGAAGGACTCCGGCGTGGAGTGGTTGGGGGAAGTGC
CGGCGCATTGGGATGTCGTGAAGTTCGTCCGGTGTGCAAAAATTGCTGAGGGTCAGGTTGATCCAAAGCAGGAGCCATAT
AGGAGCATGATGCTTGTTGCTCCAAATCACATTGAGTCAGGGACTGGACGACTCATGGCTCGTGAGACTGCAGAAGAGCA
GGGGGCAGAGAGTGGCAAGTATTATTGCTATGCTGGCGACGTAATATACAGCAAGATTCGACCGTCATTGAGAAAAGCAT
GTGTAGCCTACGAAGATTGCCTATGCAGCGCTGATATGTATCCTCTCAGGGCGCAAAGTGGGGTGTATGGCGATTATCTG
CGCTGGACGATTCTGTCTGAATCGTTCTCGACGCTAGCTTTTCTGGAATCAGAGCGCGTGGCGATGCCGAAAGTCAATCG
GGAGTCGATTGAAGAGATTCGAATCCCTATGCCGCCACCGGAAGAGCAGCTACAGATATCCCGTACCCTCGAAAAAGAAA
CGGCCCGCATCGACGCGTTGATGGAGGAGGCTGAATCGGGTATCCAGTTGCTCCAAGAACGCCGCTCCGCCCTGATCTCC
GCCGCCGTCACCGGCAAGATCGACGTGCGTGACTGGGCGCCGCCGGCCGCTGCCGAACCGGAGCAGGAACGCGAAGGAGC
GGCGCTATGA

Upstream 100 bases:

>100_bases
TGGGCTTTCCGGACGGCTGGAGGCTGGATCTCTTCTGGGCGACGGCTCAAGGTGACTGGGGCGGCCACTCTTCCGGAAAA
CCAAGTGGAAAGGAGGATAC

Downstream 100 bases:

>100_bases
GCTACGGCCGCAGCATTCGCCTCTTCCTGGTGGACGACTCCCCCCATGGGTTGATGACCGCCGAGATCGTCAACTGGACC
GGGCACGTGCTCATGGGGCC

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.EcoDI; Type I restriction enzyme EcoDI specificity protein; S protein [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSFPAYPEYKDSGVEWLGEVPEHWSVSALKRVARLESGDAISSDHISEEGEYAVYGGNGIRGFSSGYTHDGFYPLIGRQG
ALCGNVNYAKGRFWASEHAVVVWPGRQIDGFWLGELLRSMNLNQYATSAAQPGLSVETIENLYVPVPPDEEQQKIAELLD
HETARIDALIEEQQRLIELLKEKRQAVISHAVTKGLDPDVPMKDSGVEWLGEVPAHWDVVKFVRCAKIAEGQVDPKQEPY
RSMMLVAPNHIESGTGRLMARETAEEQGAESGKYYCYAGDVIYSKIRPSLRKACVAYEDCLCSADMYPLRAQSGVYGDYL
RWTILSESFSTLAFLESERVAMPKVNRESIEEIRIPMPPPEEQLQISRTLEKETARIDALMEEAESGIQLLQERRSALIS
AAVTGKIDVRDWAPPAAAEPEQEREGAAL

Sequences:

>Translated_429_residues
MSFPAYPEYKDSGVEWLGEVPEHWSVSALKRVARLESGDAISSDHISEEGEYAVYGGNGIRGFSSGYTHDGFYPLIGRQG
ALCGNVNYAKGRFWASEHAVVVWPGRQIDGFWLGELLRSMNLNQYATSAAQPGLSVETIENLYVPVPPDEEQQKIAELLD
HETARIDALIEEQQRLIELLKEKRQAVISHAVTKGLDPDVPMKDSGVEWLGEVPAHWDVVKFVRCAKIAEGQVDPKQEPY
RSMMLVAPNHIESGTGRLMARETAEEQGAESGKYYCYAGDVIYSKIRPSLRKACVAYEDCLCSADMYPLRAQSGVYGDYL
RWTILSESFSTLAFLESERVAMPKVNRESIEEIRIPMPPPEEQLQISRTLEKETARIDALMEEAESGIQLLQERRSALIS
AAVTGKIDVRDWAPPAAAEPEQEREGAAL
>Mature_428_residues
SFPAYPEYKDSGVEWLGEVPEHWSVSALKRVARLESGDAISSDHISEEGEYAVYGGNGIRGFSSGYTHDGFYPLIGRQGA
LCGNVNYAKGRFWASEHAVVVWPGRQIDGFWLGELLRSMNLNQYATSAAQPGLSVETIENLYVPVPPDEEQQKIAELLDH
ETARIDALIEEQQRLIELLKEKRQAVISHAVTKGLDPDVPMKDSGVEWLGEVPAHWDVVKFVRCAKIAEGQVDPKQEPYR
SMMLVAPNHIESGTGRLMARETAEEQGAESGKYYCYAGDVIYSKIRPSLRKACVAYEDCLCSADMYPLRAQSGVYGDYLR
WTILSESFSTLAFLESERVAMPKVNRESIEEIRIPMPPPEEQLQISRTLEKETARIDALMEEAESGIQLLQERRSALISA
AVTGKIDVRDWAPPAAAEPEQEREGAAL

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 47812; Mature: 47681

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPAYPEYKDSGVEWLGEVPEHWSVSALKRVARLESGDAISSDHISEEGEYAVYGGNGI
CCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
RGFSSGYTHDGFYPLIGRQGALCGNVNYAKGRFWASEHAVVVWPGRQIDGFWLGELLRSM
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHC
NLNQYATSAAQPGLSVETIENLYVPVPPDEEQQKIAELLDHETARIDALIEEQQRLIELL
CCHHHHHHHCCCCCCHHHHHHCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KEKRQAVISHAVTKGLDPDVPMKDSGVEWLGEVPAHWDVVKFVRCAKIAEGQVDPKQEPY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
RSMMLVAPNHIESGTGRLMARETAEEQGAESGKYYCYAGDVIYSKIRPSLRKACVAYEDC
HCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHCHHHHHHHHHHHHH
LCSADMYPLRAQSGVYGDYLRWTILSESFSTLAFLESERVAMPKVNRESIEEIRIPMPPP
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCC
EEQLQISRTLEKETARIDALMEEAESGIQLLQERRSALISAAVTGKIDVRDWAPPAAAEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
EQEREGAAL
CHHHCCCCC
>Mature Secondary Structure 
SFPAYPEYKDSGVEWLGEVPEHWSVSALKRVARLESGDAISSDHISEEGEYAVYGGNGI
CCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
RGFSSGYTHDGFYPLIGRQGALCGNVNYAKGRFWASEHAVVVWPGRQIDGFWLGELLRSM
CCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHC
NLNQYATSAAQPGLSVETIENLYVPVPPDEEQQKIAELLDHETARIDALIEEQQRLIELL
CCHHHHHHHCCCCCCHHHHHHCEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KEKRQAVISHAVTKGLDPDVPMKDSGVEWLGEVPAHWDVVKFVRCAKIAEGQVDPKQEPY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHH
RSMMLVAPNHIESGTGRLMARETAEEQGAESGKYYCYAGDVIYSKIRPSLRKACVAYEDC
HCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECHHHHHHHCHHHHHHHHHHHHH
LCSADMYPLRAQSGVYGDYLRWTILSESFSTLAFLESERVAMPKVNRESIEEIRIPMPPP
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCC
EEQLQISRTLEKETARIDALMEEAESGIQLLQERRSALISAAVTGKIDVRDWAPPAAAEP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCC
EQEREGAAL
CHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321 [H]