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The map label for this gene is slt [H]

Identifier: 121997926

GI number: 121997926

Start: 1233295

End: 1235328

Strand: Reverse

Name: slt [H]

Synonym: Hhal_1135

Alternate gene names: 121997926

Gene position: 1235328-1233295 (Counterclockwise)

Preceding gene: 121997929

Following gene: 121997925

Centisome position: 46.12

GC content: 70.65

Gene sequence:

>2034_bases
ATGGTCCCTTCCGGCGGTTATCGTGGCTTCGTCCTGGTCGCACTGTTCCTGGTCCTCGGCCTCTCCGCAGGGGTCGCCGG
TCCGGATCGGGGCTGTCCGGACGCCGACCACGGTGAACGCTTCCACGAGGCCCTGGAGGCGGCCCGGGACGATGATTGGT
CCCGGGTAGCGGAGCTGGAGCCCTGCCTGGGTGATCCGCACCCGATGGCTGCTTACCTGGAGTTCCACCGCCTGCGCAGC
GCCCTGCCCGAGCTCGATCCCCGGCGGGTGGTGGATTATCAACAGCGCCATGCCGATACGCCGCTGGCCGATGTCCTCAA
GCGCGTCGCCGTGCGCCGTTACGCCGAGGCCGGGGATGACGCGGCGGTGCGTCGGGTCACCGACGAGCCGCCGTCCCGCC
TGGCGTTGCGTTGCCACTGGTGGCAGGCCCATCTGGAGACGCGCCGGGGCGAGGCCCTGGCCTTTGCCCGGAGTGCCTGG
CATGCCGGGGTGTCGCGGCCGGCAGCCTGCGACGGCCTGTTCGAGGCGGCCCGCGAGGCTGGCGTCATCGATGATCGGGC
CGTCTGGCAGCGCATGCGCCTGGCCTTCCGCAATGACCAGCCGGGACTGCTGCGTTATTTGGAAGGTCTGCTCGGCGGCG
AGCGTTGGTTGGCTGCCGCCGAGTGGCTGTACCGTCTTGAGCAGCAGCCGACGCGGGTCGGTGACGTGGATGAGGGGTTG
CCACCGCGCCTGCGCCGTCAGGTGACCGCCGACGGGCTCTACCGGCTGGCCCGGGAAGACACGGTGGCGGCCCGCGAGTA
CCTGGAACGGATCGGGGAGGATCTGCCGGATCTACCGGCCGGCGAGCGCCGGGAGGTGTTCGAGCGCGTCGCCTGGTACT
CGATCATCCGCGGCGTCCCCGAGAACCGGGGGTGGGTCGACCGCTGGCTGGCCGAGCACGGCGGCGAGGAACTCCTGGAG
CAGCGCATGCGCCGCGCGGTGATCGAGGAGCACTGGACCGACCTGGTCTTCTGGTCGGCGCGCCTGTCGGAGGGGTCCGT
CCGCACCGCCCGTTGGCAGTACTGGCTCGGGCGGGCCCAACTCGAGTTGGGCGATGCGGCACGGGCTCGGGAGCATTGGC
GAGAGGCGGCCGGGCAGCGCTCCTTCTGGGGGTTCCTGGCCGCCGAGCACATCGGTCAAGCCTATGCGCTACAGGAAGTG
AGCTCGGAGCCGGATCCGCCCGCTCCGGATCCGGCGTTGCTGCGGGTGCGCCTGCTCTACCAGGTCGGGGAGTTGCGTCT
GGCCCGGGACGAATGGGGCCGCCTGTTGCAGGATCGCGGTGCGGCCGAGCAGCAAGCCCTGGCGGCCCACGCCTACAGGC
AGGGCTGGTATGAGTTGGCCATAGCGGCTGCCAATCGGGCCGGAGCGGTCGAGGCCCTGCAGTGGCGCTTCCCCCACGCC
TACCGCGACGCCTTCCAGCGGGTGGCGGCGGATACCGGCGTCGAATCGTGCCTGTTGATGGCAGTGGCTCGACGCGAATC
GGCCTTCCATCGTGATGCCGTCTCTCCGGCCGGGGCCCGCGGACTGATGCAGCTGCTGCCAGGAACCGCGCGGCAGTTGG
CCGAGGAGGGTGGCCGGCCAGGGCCGGATGTGGGCGGACTATTCGATCCCGAGCTCAATGTTCGCCTGGGCGCCGAGTAC
CTGCAGAAGCTGCTGGAGCGGTTCGATGGCAACCGGCTCAAGGCCCTGGCGGCCTACAATGCCGGCCCGAGCCGGGTAGC
CGGTTGGCTGGAGCAGGAAGGGCGGCCGCTGGACGTGTGGATCGAATCGATCCCCTACCATGAGACCCGCCGCTATGTGC
AGGCGGTGCTGGCCTACCGGACCATCCTGGCCCATCGTGGCGGGCTGGAGCAGGTGGCGTCAGTGGTCTCCCGCGACGAG
GTGGCCCAGGTGTACGGTGAGGCCGTCGACGGTCGCGAACCGGCGGCGGTGCGCCTGAGCCGGAACCCGATGGGTGAAGC
GATGCCCCCGGGGGATGGCCGCCGGGAAGACTGA

Upstream 100 bases:

>100_bases
CCTGCGTGCGGCGGCCTGTGCAGTTGACCTGCGGGGGTGCCGGCGCTAGCGTTCGGGGAGGCTTGGCTATCACGCGCACC
CGCGCCGGAGTCCCGAGAGC

Downstream 100 bases:

>100_bases
GTTGCCCCCTGTGCCCCTGTTACCCTGATCCGGGTTGGCCCGGCGCGGCTTGGCCGCGCCGGGGCGTATCCGTGTGTGGA
GTCGCACCAGCGTTAGGGAA

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MVPSGGYRGFVLVALFLVLGLSAGVAGPDRGCPDADHGERFHEALEAARDDDWSRVAELEPCLGDPHPMAAYLEFHRLRS
ALPELDPRRVVDYQQRHADTPLADVLKRVAVRRYAEAGDDAAVRRVTDEPPSRLALRCHWWQAHLETRRGEALAFARSAW
HAGVSRPAACDGLFEAAREAGVIDDRAVWQRMRLAFRNDQPGLLRYLEGLLGGERWLAAAEWLYRLEQQPTRVGDVDEGL
PPRLRRQVTADGLYRLAREDTVAAREYLERIGEDLPDLPAGERREVFERVAWYSIIRGVPENRGWVDRWLAEHGGEELLE
QRMRRAVIEEHWTDLVFWSARLSEGSVRTARWQYWLGRAQLELGDAARAREHWREAAGQRSFWGFLAAEHIGQAYALQEV
SSEPDPPAPDPALLRVRLLYQVGELRLARDEWGRLLQDRGAAEQQALAAHAYRQGWYELAIAAANRAGAVEALQWRFPHA
YRDAFQRVAADTGVESCLLMAVARRESAFHRDAVSPAGARGLMQLLPGTARQLAEEGGRPGPDVGGLFDPELNVRLGAEY
LQKLLERFDGNRLKALAAYNAGPSRVAGWLEQEGRPLDVWIESIPYHETRRYVQAVLAYRTILAHRGGLEQVASVVSRDE
VAQVYGEAVDGREPAAVRLSRNPMGEAMPPGDGRRED

Sequences:

>Translated_677_residues
MVPSGGYRGFVLVALFLVLGLSAGVAGPDRGCPDADHGERFHEALEAARDDDWSRVAELEPCLGDPHPMAAYLEFHRLRS
ALPELDPRRVVDYQQRHADTPLADVLKRVAVRRYAEAGDDAAVRRVTDEPPSRLALRCHWWQAHLETRRGEALAFARSAW
HAGVSRPAACDGLFEAAREAGVIDDRAVWQRMRLAFRNDQPGLLRYLEGLLGGERWLAAAEWLYRLEQQPTRVGDVDEGL
PPRLRRQVTADGLYRLAREDTVAAREYLERIGEDLPDLPAGERREVFERVAWYSIIRGVPENRGWVDRWLAEHGGEELLE
QRMRRAVIEEHWTDLVFWSARLSEGSVRTARWQYWLGRAQLELGDAARAREHWREAAGQRSFWGFLAAEHIGQAYALQEV
SSEPDPPAPDPALLRVRLLYQVGELRLARDEWGRLLQDRGAAEQQALAAHAYRQGWYELAIAAANRAGAVEALQWRFPHA
YRDAFQRVAADTGVESCLLMAVARRESAFHRDAVSPAGARGLMQLLPGTARQLAEEGGRPGPDVGGLFDPELNVRLGAEY
LQKLLERFDGNRLKALAAYNAGPSRVAGWLEQEGRPLDVWIESIPYHETRRYVQAVLAYRTILAHRGGLEQVASVVSRDE
VAQVYGEAVDGREPAAVRLSRNPMGEAMPPGDGRRED
>Mature_677_residues
MVPSGGYRGFVLVALFLVLGLSAGVAGPDRGCPDADHGERFHEALEAARDDDWSRVAELEPCLGDPHPMAAYLEFHRLRS
ALPELDPRRVVDYQQRHADTPLADVLKRVAVRRYAEAGDDAAVRRVTDEPPSRLALRCHWWQAHLETRRGEALAFARSAW
HAGVSRPAACDGLFEAAREAGVIDDRAVWQRMRLAFRNDQPGLLRYLEGLLGGERWLAAAEWLYRLEQQPTRVGDVDEGL
PPRLRRQVTADGLYRLAREDTVAAREYLERIGEDLPDLPAGERREVFERVAWYSIIRGVPENRGWVDRWLAEHGGEELLE
QRMRRAVIEEHWTDLVFWSARLSEGSVRTARWQYWLGRAQLELGDAARAREHWREAAGQRSFWGFLAAEHIGQAYALQEV
SSEPDPPAPDPALLRVRLLYQVGELRLARDEWGRLLQDRGAAEQQALAAHAYRQGWYELAIAAANRAGAVEALQWRFPHA
YRDAFQRVAADTGVESCLLMAVARRESAFHRDAVSPAGARGLMQLLPGTARQLAEEGGRPGPDVGGLFDPELNVRLGAEY
LQKLLERFDGNRLKALAAYNAGPSRVAGWLEQEGRPLDVWIESIPYHETRRYVQAVLAYRTILAHRGGLEQVASVVSRDE
VAQVYGEAVDGREPAAVRLSRNPMGEAMPPGDGRRED

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=576, Percent_Identity=31.5972222222222, Blast_Score=245, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76064; Mature: 76064

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPSGGYRGFVLVALFLVLGLSAGVAGPDRGCPDADHGERFHEALEAARDDDWSRVAELE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
PCLGDPHPMAAYLEFHRLRSALPELDPRRVVDYQQRHADTPLADVLKRVAVRRYAEAGDD
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
AAVRRVTDEPPSRLALRCHWWQAHLETRRGEALAFARSAWHAGVSRPAACDGLFEAAREA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GVIDDRAVWQRMRLAFRNDQPGLLRYLEGLLGGERWLAAAEWLYRLEQQPTRVGDVDEGL
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PPRLRRQVTADGLYRLAREDTVAAREYLERIGEDLPDLPAGERREVFERVAWYSIIRGVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ENRGWVDRWLAEHGGEELLEQRMRRAVIEEHWTDLVFWSARLSEGSVRTARWQYWLGRAQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LELGDAARAREHWREAAGQRSFWGFLAAEHIGQAYALQEVSSEPDPPAPDPALLRVRLLY
HHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QVGELRLARDEWGRLLQDRGAAEQQALAAHAYRQGWYELAIAAANRAGAVEALQWRFPHA
HHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
YRDAFQRVAADTGVESCLLMAVARRESAFHRDAVSPAGARGLMQLLPGTARQLAEEGGRP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCC
GPDVGGLFDPELNVRLGAEYLQKLLERFDGNRLKALAAYNAGPSRVAGWLEQEGRPLDVW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHEECCCCHHHHHHHHHHCCCCHHHH
IESIPYHETRRYVQAVLAYRTILAHRGGLEQVASVVSRDEVAQVYGEAVDGREPAAVRLS
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
RNPMGEAMPPGDGRRED
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVPSGGYRGFVLVALFLVLGLSAGVAGPDRGCPDADHGERFHEALEAARDDDWSRVAELE
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHC
PCLGDPHPMAAYLEFHRLRSALPELDPRRVVDYQQRHADTPLADVLKRVAVRRYAEAGDD
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
AAVRRVTDEPPSRLALRCHWWQAHLETRRGEALAFARSAWHAGVSRPAACDGLFEAAREA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
GVIDDRAVWQRMRLAFRNDQPGLLRYLEGLLGGERWLAAAEWLYRLEQQPTRVGDVDEGL
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PPRLRRQVTADGLYRLAREDTVAAREYLERIGEDLPDLPAGERREVFERVAWYSIIRGVP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
ENRGWVDRWLAEHGGEELLEQRMRRAVIEEHWTDLVFWSARLSEGSVRTARWQYWLGRAQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LELGDAARAREHWREAAGQRSFWGFLAAEHIGQAYALQEVSSEPDPPAPDPALLRVRLLY
HHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QVGELRLARDEWGRLLQDRGAAEQQALAAHAYRQGWYELAIAAANRAGAVEALQWRFPHA
HHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHH
YRDAFQRVAADTGVESCLLMAVARRESAFHRDAVSPAGARGLMQLLPGTARQLAEEGGRP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCC
GPDVGGLFDPELNVRLGAEYLQKLLERFDGNRLKALAAYNAGPSRVAGWLEQEGRPLDVW
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHEECCCCHHHHHHHHHHCCCCHHHH
IESIPYHETRRYVQAVLAYRTILAHRGGLEQVASVVSRDEVAQVYGEAVDGREPAAVRLS
HHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
RNPMGEAMPPGDGRRED
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]