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The map label for this gene is otsA [H]

Identifier: 121997911

GI number: 121997911

Start: 1216041

End: 1217411

Strand: Reverse

Name: otsA [H]

Synonym: Hhal_1120

Alternate gene names: 121997911

Gene position: 1217411-1216041 (Counterclockwise)

Preceding gene: 121997912

Following gene: 121997910

Centisome position: 45.45

GC content: 66.3

Gene sequence:

>1371_bases
GTGAGTCGACTGGTAACCGTTTCCAATCGTGTGGCCTTGCCCAGCCAGCTGCAGGCCGCGCAGGGTGGTCTGGCCGTCGG
CCTGCGTTCGGCGCTGGAGGAGTCCGGTGGCATGTGGTTCGGTTGGGACGGCGGCGTGGACGAACGCATCGACGGGTTGC
GCCAGCCCCGGGTCCAGACCGCCAACGGGGTGCGCTACGCCACCCTGCGCCTGAGTCGCCTCGAGTACGATCGCTACTAC
CTGGGCTACGCCAACCAGGTGCTCTGGCCGCTGTTCCACTACCGCATGTCCTTCGTCCACTGCCGGCGCGAGCGCATCGA
GGGCTACTGGGAGGTCAACCGGCTGTTTGCCGAGCATCTGCCACCGTTGCTCGAGGGCGACGAGATCATCTGGGTCCACG
ACTACCACTTCATTCCTCTCGGGCAACTGTTGCGCGAGCAGGGTGTCGAGGCCCCCATTGGTTTCTTCCTGCACACCCCC
TTCCCGCCCTGGGACGTCTTCCGCGCCCTGCCCGGTCACGAGCCACTGCTCGAGGCGCTGTGCCGGTACGATCTGGTCGG
GTTCCAGACGCGCATCGACCGGGACAACTTCCTCGATTGCCTGACCCACTACCGCCCGCAGCTGCAGCGCCCACGGGCCG
AGGTGTTCCCCATCAGCATCGATGTCGATCAGGTGGCCCGGGAGGCGCAGCGGGGCTACAACTCCCAGCAGGGGCGGCGG
CTGCAGCAGAGCCTGCGCGACCGTCGGTTGATGATCGGCGTCGACCGGCTCGATTACAGCAAGGGCCTGCGCAACCGGTT
CGAGGCCTACGAGGCGCTGCTCGAGCAGCACAGTGAGCACCGCGGGGACGTGGTCTTCCTGCAGATCGCCCCGGTCTCCC
GTGGCGATGTACCCGAGTACGAGGAGATCCGCCAATACCTGGAGTACCTGGCTGGCCACATCAACGGTCGTTTCGCCGAG
TACGACTGGGTGCCGCTGCGTTACCTCAATCGCGGTTTCCACCGTTCGAATATCCTCGGCTTCCTGGCGCGTAGCGACGT
CGGGCTGATCACCCCCATGCGTGACGGCATGAATCTGGTGGCCAAGGAGTTTGTCGCCGCCCAGGATCCCGGCGATCCGG
GGGCGCTGGTGCTGTCGCGCTACGCTGGCGCTGCCGAAGAGCTCGATGGCGCGGTGCTGGTCAATCCCTACGACGTGGAT
CAGATGGTTGATGCCATGCACCAGGCGCTGACCATGCCGCTGGGGGAGCGGCGCGAGCGCTGGCAGCAGATGATGGACGC
GCTACGCCGACAGGACGTGCATCGCTGGCGGAAGGATTTCATCCAGGCCCTGCACGATGCCCACCGCGCACGGGGTTCGG
AGGCGCTGTGA

Upstream 100 bases:

>100_bases
CGTACACCGGAGAACTCTGGGGGAACTTCCCGCAGACCTACAGCATGGTCGGTCTGATCAACTCCGCAATGCGCCTGAGT
CGCAGTTGGGAGGAACCGCT

Downstream 100 bases:

>100_bases
CCCGTTACCTGCTGGCCGATATCGGCGGCACGCACACCCGCATCGCAACGGCCGGGCCGGGCGAGGCGCCGGGGACGGCG
ACGCGGTACCGCAATCGGGA

Product: Alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSRLVTVSNRVALPSQLQAAQGGLAVGLRSALEESGGMWFGWDGGVDERIDGLRQPRVQTANGVRYATLRLSRLEYDRYY
LGYANQVLWPLFHYRMSFVHCRRERIEGYWEVNRLFAEHLPPLLEGDEIIWVHDYHFIPLGQLLREQGVEAPIGFFLHTP
FPPWDVFRALPGHEPLLEALCRYDLVGFQTRIDRDNFLDCLTHYRPQLQRPRAEVFPISIDVDQVAREAQRGYNSQQGRR
LQQSLRDRRLMIGVDRLDYSKGLRNRFEAYEALLEQHSEHRGDVVFLQIAPVSRGDVPEYEEIRQYLEYLAGHINGRFAE
YDWVPLRYLNRGFHRSNILGFLARSDVGLITPMRDGMNLVAKEFVAAQDPGDPGALVLSRYAGAAEELDGAVLVNPYDVD
QMVDAMHQALTMPLGERRERWQQMMDALRRQDVHRWRKDFIQALHDAHRARGSEAL

Sequences:

>Translated_456_residues
MSRLVTVSNRVALPSQLQAAQGGLAVGLRSALEESGGMWFGWDGGVDERIDGLRQPRVQTANGVRYATLRLSRLEYDRYY
LGYANQVLWPLFHYRMSFVHCRRERIEGYWEVNRLFAEHLPPLLEGDEIIWVHDYHFIPLGQLLREQGVEAPIGFFLHTP
FPPWDVFRALPGHEPLLEALCRYDLVGFQTRIDRDNFLDCLTHYRPQLQRPRAEVFPISIDVDQVAREAQRGYNSQQGRR
LQQSLRDRRLMIGVDRLDYSKGLRNRFEAYEALLEQHSEHRGDVVFLQIAPVSRGDVPEYEEIRQYLEYLAGHINGRFAE
YDWVPLRYLNRGFHRSNILGFLARSDVGLITPMRDGMNLVAKEFVAAQDPGDPGALVLSRYAGAAEELDGAVLVNPYDVD
QMVDAMHQALTMPLGERRERWQQMMDALRRQDVHRWRKDFIQALHDAHRARGSEAL
>Mature_455_residues
SRLVTVSNRVALPSQLQAAQGGLAVGLRSALEESGGMWFGWDGGVDERIDGLRQPRVQTANGVRYATLRLSRLEYDRYYL
GYANQVLWPLFHYRMSFVHCRRERIEGYWEVNRLFAEHLPPLLEGDEIIWVHDYHFIPLGQLLREQGVEAPIGFFLHTPF
PPWDVFRALPGHEPLLEALCRYDLVGFQTRIDRDNFLDCLTHYRPQLQRPRAEVFPISIDVDQVAREAQRGYNSQQGRRL
QQSLRDRRLMIGVDRLDYSKGLRNRFEAYEALLEQHSEHRGDVVFLQIAPVSRGDVPEYEEIRQYLEYLAGHINGRFAEY
DWVPLRYLNRGFHRSNILGFLARSDVGLITPMRDGMNLVAKEFVAAQDPGDPGALVLSRYAGAAEELDGAVLVNPYDVDQ
MVDAMHQALTMPLGERRERWQQMMDALRRQDVHRWRKDFIQALHDAHRARGSEAL

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=455, Percent_Identity=48.1318681318681, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI115533558, Length=351, Percent_Identity=30.1994301994302, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI115533560, Length=351, Percent_Identity=30.1994301994302, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI71987012, Length=431, Percent_Identity=29.0023201856148, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319602, Length=471, Percent_Identity=32.484076433121, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6323917, Length=383, Percent_Identity=27.154046997389, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6323537, Length=386, Percent_Identity=28.4974093264249, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320279, Length=398, Percent_Identity=24.6231155778894, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19920676, Length=489, Percent_Identity=34.3558282208589, Blast_Score=265, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52836; Mature: 52705

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVTVSNRVALPSQLQAAQGGLAVGLRSALEESGGMWFGWDGGVDERIDGLRQPRVQT
CCCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCC
ANGVRYATLRLSRLEYDRYYLGYANQVLWPLFHYRMSFVHCRRERIEGYWEVNRLFAEHL
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PPLLEGDEIIWVHDYHFIPLGQLLREQGVEAPIGFFLHTPFPPWDVFRALPGHEPLLEAL
CCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCHHHHHH
CRYDLVGFQTRIDRDNFLDCLTHYRPQLQRPRAEVFPISIDVDQVAREAQRGYNSQQGRR
HHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCCCEEEEEECHHHHHHHHHHCCCCHHHHH
LQQSLRDRRLMIGVDRLDYSKGLRNRFEAYEALLEQHSEHRGDVVFLQIAPVSRGDVPEY
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCH
EEIRQYLEYLAGHINGRFAEYDWVPLRYLNRGFHRSNILGFLARSDVGLITPMRDGMNLV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEECCCHHHHHHH
AKEFVAAQDPGDPGALVLSRYAGAAEELDGAVLVNPYDVDQMVDAMHQALTMPLGERRER
HHHHHHCCCCCCCHHHHHHHHCCCHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHH
WQQMMDALRRQDVHRWRKDFIQALHDAHRARGSEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SRLVTVSNRVALPSQLQAAQGGLAVGLRSALEESGGMWFGWDGGVDERIDGLRQPRVQT
CCEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCC
ANGVRYATLRLSRLEYDRYYLGYANQVLWPLFHYRMSFVHCRRERIEGYWEVNRLFAEHL
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PPLLEGDEIIWVHDYHFIPLGQLLREQGVEAPIGFFLHTPFPPWDVFRALPGHEPLLEAL
CCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCCCCHHHHHH
CRYDLVGFQTRIDRDNFLDCLTHYRPQLQRPRAEVFPISIDVDQVAREAQRGYNSQQGRR
HHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCCCCEEEEEECHHHHHHHHHHCCCCHHHHH
LQQSLRDRRLMIGVDRLDYSKGLRNRFEAYEALLEQHSEHRGDVVFLQIAPVSRGDVPEY
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCH
EEIRQYLEYLAGHINGRFAEYDWVPLRYLNRGFHRSNILGFLARSDVGLITPMRDGMNLV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCEECCCHHHHHHH
AKEFVAAQDPGDPGALVLSRYAGAAEELDGAVLVNPYDVDQMVDAMHQALTMPLGERRER
HHHHHHCCCCCCCHHHHHHHHCCCHHHCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHH
WQQMMDALRRQDVHRWRKDFIQALHDAHRARGSEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA