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The map label for this gene is gacS [H]

Identifier: 121997909

GI number: 121997909

Start: 1213741

End: 1215054

Strand: Reverse

Name: gacS [H]

Synonym: Hhal_1118

Alternate gene names: 121997909

Gene position: 1215054-1213741 (Counterclockwise)

Preceding gene: 121997910

Following gene: 121997908

Centisome position: 45.36

GC content: 68.8

Gene sequence:

>1314_bases
ATGGCATCTTCCCGGATATCCCCGGACGAGGCGCATCGCCTCGAGGCCCTGGCGCGCTACCAGGGAGGAGCGGATGGGCC
CGAGGTGGCCTTTGAGCGCCTTGTCGGCCTGTGTGCGCGGATCTTTGATGTGCCCATCGCCTTCATCTCGCTGATCGAGC
GCGAGCGGCAATGGTTCAAGGCAGCCCGCGGGCTGGGGATCGAGGAGACGCCGCGCGAGATCGCCTTTTGCAACTGCGTG
ATCCAGACCGGGGACCCGCTGGTGGTCACCGATACCCATGCGGATCCGCGTTTCGCCGACCACCCGCTGGTTACTGGGCC
CCCGCACCTGCGTTTCTACGCCGGGGTGCCGTTGGTGGTTGAGGGTGGGGCGCGCATCGGCACCCTCTGCCTCGGCGACC
GCCAGGCCCGCACCCTGTCCGACGCCGAGCTGGCGACCCTCCAGGAGCTGGCGGAGGTGGTGGTCGACGAGTTCGAGCTG
CGCCGGGCCAACCAGCGCCTCGATGAGGAGCGGGCGCGAGCCGAACGGTCCGCGATCGAGCTGCGCCGTATCAACCTCGA
GCTGCAGCAGGCCCGCGAGGAGGCCGAGCGCGGTAACCGGGCCAAGAGCCAGTTCCTGTCGAGCATGAGTCACGAGTTGC
GGACACCGCTTAACGCGGTGCTCGGCTTTGCCCAGATCCTCGAGCAGGATCCCCGCGAGCCGCTTTCCGAGCGCCAGGCC
GGTTACGTCGATCAGATCCTCGGGGGTGGCCGTCACCTGCTGTCGCTGATCGATGACATCCTCGATCTTTCCCGCGTCGA
GGCCGGTCGGGTGGAACTCAAGCCGGAGTGGGTGGCGCCGGGCCTGCTGGTCGACCAGTGTGCCGAGCTGCTCGGTCCGA
TGGCCACCGACGCCGGGGTGCACCTGTCCGTGTACGTGGCCGAGGCCGTTCCGGCATCCATCTGGGTGGATCCACGGCGC
ATCCGCCAGGTGCTGTTCAACCTCGCCAGCAATGCCGTCAAGTACAACCGGCCGGACGGGCGGGTGGAGGTCGAGGTCGA
GGCCCCGGCGCCCGACCGGATCCGCTTCCGGGTGCGGGACACCGGGATCGGTATCCGCGAGGAGCGCCAGCGCGAGGTCT
TCTACCCCTTCAACCGCCTGGGTCTGGAGTCCTCGGAGGTGGAGGGGACCGGCATCGGTCTGGTGGTGACCCGCCGCCTG
GTGGAGCAGATGGGCGGCGAGATCGGTTTCTCCAGCGCGCCCGGCGTGGGGTCGGTCTTCTGGGTCGATCTGCCCGGCGT
GGTGACCGCGGACGAGGTCTCGAGCGATGGATAG

Upstream 100 bases:

>100_bases
GGCCGCGCTGCTCGGCCTGCGGGCGTGGCTCGATGACTTGCGGTACCCTTGAGCCAGCGCTGGTTTTTGAGCATCCCGTT
GCACGCGAGGTCGGCTGACC

Downstream 100 bases:

>100_bases
ACCCAGCTACGAGGGCAGCGGCCTGGTCAACCTGATGGCGTCCCTGGGCCGTGCCTTCGGGGCGGCGTCCAGCCACTATC
CGGCCCTGGATCCTGAGCCG

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MASSRISPDEAHRLEALARYQGGADGPEVAFERLVGLCARIFDVPIAFISLIERERQWFKAARGLGIEETPREIAFCNCV
IQTGDPLVVTDTHADPRFADHPLVTGPPHLRFYAGVPLVVEGGARIGTLCLGDRQARTLSDAELATLQELAEVVVDEFEL
RRANQRLDEERARAERSAIELRRINLELQQAREEAERGNRAKSQFLSSMSHELRTPLNAVLGFAQILEQDPREPLSERQA
GYVDQILGGGRHLLSLIDDILDLSRVEAGRVELKPEWVAPGLLVDQCAELLGPMATDAGVHLSVYVAEAVPASIWVDPRR
IRQVLFNLASNAVKYNRPDGRVEVEVEAPAPDRIRFRVRDTGIGIREERQREVFYPFNRLGLESSEVEGTGIGLVVTRRL
VEQMGGEIGFSSAPGVGSVFWVDLPGVVTADEVSSDG

Sequences:

>Translated_437_residues
MASSRISPDEAHRLEALARYQGGADGPEVAFERLVGLCARIFDVPIAFISLIERERQWFKAARGLGIEETPREIAFCNCV
IQTGDPLVVTDTHADPRFADHPLVTGPPHLRFYAGVPLVVEGGARIGTLCLGDRQARTLSDAELATLQELAEVVVDEFEL
RRANQRLDEERARAERSAIELRRINLELQQAREEAERGNRAKSQFLSSMSHELRTPLNAVLGFAQILEQDPREPLSERQA
GYVDQILGGGRHLLSLIDDILDLSRVEAGRVELKPEWVAPGLLVDQCAELLGPMATDAGVHLSVYVAEAVPASIWVDPRR
IRQVLFNLASNAVKYNRPDGRVEVEVEAPAPDRIRFRVRDTGIGIREERQREVFYPFNRLGLESSEVEGTGIGLVVTRRL
VEQMGGEIGFSSAPGVGSVFWVDLPGVVTADEVSSDG
>Mature_436_residues
ASSRISPDEAHRLEALARYQGGADGPEVAFERLVGLCARIFDVPIAFISLIERERQWFKAARGLGIEETPREIAFCNCVI
QTGDPLVVTDTHADPRFADHPLVTGPPHLRFYAGVPLVVEGGARIGTLCLGDRQARTLSDAELATLQELAEVVVDEFELR
RANQRLDEERARAERSAIELRRINLELQQAREEAERGNRAKSQFLSSMSHELRTPLNAVLGFAQILEQDPREPLSERQAG
YVDQILGGGRHLLSLIDDILDLSRVEAGRVELKPEWVAPGLLVDQCAELLGPMATDAGVHLSVYVAEAVPASIWVDPRRI
RQVLFNLASNAVKYNRPDGRVEVEVEAPAPDRIRFRVRDTGIGIREERQREVFYPFNRLGLESSEVEGTGIGLVVTRRLV
EQMGGEIGFSSAPGVGSVFWVDLPGVVTADEVSSDG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=245, Percent_Identity=37.1428571428571, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=35.6060606060606, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788713, Length=250, Percent_Identity=30, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI145693157, Length=265, Percent_Identity=32.4528301886792, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994928, Length=357, Percent_Identity=28.2913165266106, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1786912, Length=393, Percent_Identity=27.4809160305344, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790436, Length=244, Percent_Identity=27.0491803278689, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082128, Length=231, Percent_Identity=29.4372294372294, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=334, Percent_Identity=24.5508982035928, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788393, Length=248, Percent_Identity=23.3870967741935, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=27.9661016949153, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790861, Length=229, Percent_Identity=25.3275109170306, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788279, Length=226, Percent_Identity=25.6637168141593, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48234; Mature: 48103

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRISPDEAHRLEALARYQGGADGPEVAFERLVGLCARIFDVPIAFISLIERERQWFK
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARGLGIEETPREIAFCNCVIQTGDPLVVTDTHADPRFADHPLVTGPPHLRFYAGVPLVV
HHHCCCCCCCCHHHEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEE
EGGARIGTLCLGDRQARTLSDAELATLQELAEVVVDEFELRRANQRLDEERARAERSAIE
ECCCEEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRINLELQQAREEAERGNRAKSQFLSSMSHELRTPLNAVLGFAQILEQDPREPLSERQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHC
GYVDQILGGGRHLLSLIDDILDLSRVEAGRVELKPEWVAPGLLVDQCAELLGPMATDAGV
CCHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
HLSVYVAEAVPASIWVDPRRIRQVLFNLASNAVKYNRPDGRVEVEVEAPAPDRIRFRVRD
EEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCEEEEEEC
TGIGIREERQREVFYPFNRLGLESSEVEGTGIGLVVTRRLVEQMGGEIGFSSAPGVGSVF
CCCCCHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
WVDLPGVVTADEVSSDG
EEECCCEEECCCCCCCC
>Mature Secondary Structure 
ASSRISPDEAHRLEALARYQGGADGPEVAFERLVGLCARIFDVPIAFISLIERERQWFK
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARGLGIEETPREIAFCNCVIQTGDPLVVTDTHADPRFADHPLVTGPPHLRFYAGVPLVV
HHHCCCCCCCCHHHEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEE
EGGARIGTLCLGDRQARTLSDAELATLQELAEVVVDEFELRRANQRLDEERARAERSAIE
ECCCEEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRRINLELQQAREEAERGNRAKSQFLSSMSHELRTPLNAVLGFAQILEQDPREPLSERQA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHC
GYVDQILGGGRHLLSLIDDILDLSRVEAGRVELKPEWVAPGLLVDQCAELLGPMATDAGV
CCHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
HLSVYVAEAVPASIWVDPRRIRQVLFNLASNAVKYNRPDGRVEVEVEAPAPDRIRFRVRD
EEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCEEEEEEC
TGIGIREERQREVFYPFNRLGLESSEVEGTGIGLVVTRRLVEQMGGEIGFSSAPGVGSVF
CCCCCHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
WVDLPGVVTADEVSSDG
EEECCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]