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The map label for this gene is cspA [H]

Identifier: 121997901

GI number: 121997901

Start: 1208538

End: 1208747

Strand: Reverse

Name: cspA [H]

Synonym: Hhal_1110

Alternate gene names: 121997901

Gene position: 1208747-1208538 (Counterclockwise)

Preceding gene: 121997905

Following gene: 121997900

Centisome position: 45.13

GC content: 63.33

Gene sequence:

>210_bases
ATGTCAGAGCGTCAACAAGGTACGGTTAAGTGGTTCGACAACGCCAAGGGCTACGGCTTCATCAGCCGCGAAGGGGGTGA
AGACGTGTTCGTCCACTTCCGTGCCATCCGGGGCGACGGTTTCCGCTCCCTGGACGAGGGTCAAGCGGTCGAGTTCAGCG
TGACCCGCAGCCCGAAGGGCCTGCAGGCCGAGGACGTCGCCGCGGTCTAA

Upstream 100 bases:

>100_bases
CTTTGGCTTTGTGCCAATATATGGGGTGCGACCGCCAGTAAGACCTGACCCTGGTCCCGTGGAGGTCGCGTCACGATGAT
TAATACAGAGGTTGTTTGAG

Downstream 100 bases:

>100_bases
CCCGCTGCCAGAGCGGTAGTAAGCTGCAAGTGAGAGAAAGGCCCGCCCCCGGCGGGCCTTTCTCGTTGCGGGGGTGGTGT
TCGAGCGTGTGTGCCCCCGC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MSERQQGTVKWFDNAKGYGFISREGGEDVFVHFRAIRGDGFRSLDEGQAVEFSVTRSPKGLQAEDVAAV

Sequences:

>Translated_69_residues
MSERQQGTVKWFDNAKGYGFISREGGEDVFVHFRAIRGDGFRSLDEGQAVEFSVTRSPKGLQAEDVAAV
>Mature_68_residues
SERQQGTVKWFDNAKGYGFISREGGEDVFVHFRAIRGDGFRSLDEGQAVEFSVTRSPKGLQAEDVAAV

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=66, Percent_Identity=53.030303030303, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1786841, Length=67, Percent_Identity=52.2388059701493, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1789979, Length=70, Percent_Identity=50, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787834, Length=70, Percent_Identity=48.5714285714286, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI1788126, Length=67, Percent_Identity=50.7462686567164, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1787839, Length=67, Percent_Identity=52.2388059701493, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=47.1428571428571, Blast_Score=70, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24658520, Length=63, Percent_Identity=47.6190476190476, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7638; Mature: 7507

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERQQGTVKWFDNAKGYGFISREGGEDVFVHFRAIRGDGFRSLDEGQAVEFSVTRSPKG
CCCCCCCCEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCC
LQAEDVAAV
CCCCCEECC
>Mature Secondary Structure 
SERQQGTVKWFDNAKGYGFISREGGEDVFVHFRAIRGDGFRSLDEGQAVEFSVTRSPKG
CCCCCCCEEEECCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCC
LQAEDVAAV
CCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]