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The map label for this gene is prpB

Identifier: 121997863

GI number: 121997863

Start: 1164605

End: 1165510

Strand: Reverse

Name: prpB

Synonym: Hhal_1072

Alternate gene names: 121997863

Gene position: 1165510-1164605 (Counterclockwise)

Preceding gene: 121997864

Following gene: 121997862

Centisome position: 43.51

GC content: 68.54

Gene sequence:

>906_bases
ATGACAGAGCAGTCATCCGCCGGGCGGCGGTTCCGCGACGCGGTCGCGGACGAACGCCCGCTGCAAGTGGTGGGGGCCAT
CAATGCCTACTCGGCACTGCTTGCCGAGCGGGCCGGGTTCCGGGCGCTGTATGTCTCGGGGGCGGGGGTGGCCAATGCCT
CCTACGGTCTGCCGGATCTGGGGATCACTCAGCTGGCGGACGTCGTCGAGGATGTGCGGCGCATCACCGACGCGACCCGT
ACCCCGGTGCTGGTGGATATCGACACCGGCTTCGGCGGGGCCTTCAGTGTGGCCCGCACCGTCCGGGAGCTGACGCGAGC
CGGGGCGGCCGCGGTGCACATCGAGGATCAGGTCCAGGCCAAGCGCTGTGGGCACCGCCCGGGCAAGGAACTGGTCTCCA
CCGCCGAGATGGTCGACCGGGTGAAGGCGGCGGTGGATGCCCGTACGGACCCCGAGCTGGTGGTCATGGCGCGGACCGAT
GCCCGGGCCTCCGAGGGGCTCGAGGCGGCCGTGGAGCGCGCTGAGGCCTATGTGGAGGCCGGCGCCGATATGGTCTTCGC
CGAGGCCATGCACAGTCTGGACGATATCCGCGCCTTCACCGACCGCGTGCCGGTGCCGGTGCTGGCCAACATCACCGAGT
TCGGCAAGACCCCGTACTTTACGGTGGAGGAGTTGCGCGAGGCGGGTGCGGGCCTGGTCCTGTATCCGCTGTCGGCCTTC
CGTGCCATGAGCCGGGCCGCTGAAGCGGTCTACGGTGCCATCCGTGCTGACGGTACCCAGGAGCGGGTGCTCGATCGCAT
GCAAACGCGCGAGGAACTCTACGAGGTACTCGGTTATCACGACTACGAGCGCAAGCTCGATGAGCTCTTCGGGCGCGACG
GACGAACAGGAGGAGGACAGCGATGA

Upstream 100 bases:

>100_bases
CGGACCCTGGTCCGGGTCGGCGGCGATACGGTCGCCTGATCCCGATCGCCTGATCCGAGCGATCCGCTGAATCCGATCAC
CACACGGAGCGAACAGAAAC

Downstream 100 bases:

>100_bases
CGGGTAAGAAGACGGGTGCCGGGCTGCGCGGCCAATCCGCCGGGGAGACGGCGCTGTGTACGGTGGGGCAGGAGGGGACC
GGCCTGACCTATCGCGGCTA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTEQSSAGRRFRDAVADERPLQVVGAINAYSALLAERAGFRALYVSGAGVANASYGLPDLGITQLADVVEDVRRITDATR
TPVLVDIDTGFGGAFSVARTVRELTRAGAAAVHIEDQVQAKRCGHRPGKELVSTAEMVDRVKAAVDARTDPELVVMARTD
ARASEGLEAAVERAEAYVEAGADMVFAEAMHSLDDIRAFTDRVPVPVLANITEFGKTPYFTVEELREAGAGLVLYPLSAF
RAMSRAAEAVYGAIRADGTQERVLDRMQTREELYEVLGYHDYERKLDELFGRDGRTGGGQR

Sequences:

>Translated_301_residues
MTEQSSAGRRFRDAVADERPLQVVGAINAYSALLAERAGFRALYVSGAGVANASYGLPDLGITQLADVVEDVRRITDATR
TPVLVDIDTGFGGAFSVARTVRELTRAGAAAVHIEDQVQAKRCGHRPGKELVSTAEMVDRVKAAVDARTDPELVVMARTD
ARASEGLEAAVERAEAYVEAGADMVFAEAMHSLDDIRAFTDRVPVPVLANITEFGKTPYFTVEELREAGAGLVLYPLSAF
RAMSRAAEAVYGAIRADGTQERVLDRMQTREELYEVLGYHDYERKLDELFGRDGRTGGGQR
>Mature_300_residues
TEQSSAGRRFRDAVADERPLQVVGAINAYSALLAERAGFRALYVSGAGVANASYGLPDLGITQLADVVEDVRRITDATRT
PVLVDIDTGFGGAFSVARTVRELTRAGAAAVHIEDQVQAKRCGHRPGKELVSTAEMVDRVKAAVDARTDPELVVMARTDA
RASEGLEAAVERAEAYVEAGADMVFAEAMHSLDDIRAFTDRVPVPVLANITEFGKTPYFTVEELREAGAGLVLYPLSAFR
AMSRAAEAVYGAIRADGTQERVLDRMQTREELYEVLGYHDYERKLDELFGRDGRTGGGQR

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=288, Percent_Identity=63.5416666666667, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790445, Length=263, Percent_Identity=27.7566539923954, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=189, Percent_Identity=32.2751322751323, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561814, Length=189, Percent_Identity=32.2751322751323, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320908, Length=106, Percent_Identity=32.0754716981132, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32644; Mature: 32513

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQSSAGRRFRDAVADERPLQVVGAINAYSALLAERAGFRALYVSGAGVANASYGLPDL
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
GITQLADVVEDVRRITDATRTPVLVDIDTGFGGAFSVARTVRELTRAGAAAVHIEDQVQA
CHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHH
KRCGHRPGKELVSTAEMVDRVKAAVDARTDPELVVMARTDARASEGLEAAVERAEAYVEA
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
GADMVFAEAMHSLDDIRAFTDRVPVPVLANITEFGKTPYFTVEELREAGAGLVLYPLSAF
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHHHCCCCEEEHHHHHH
RAMSRAAEAVYGAIRADGTQERVLDRMQTREELYEVLGYHDYERKLDELFGRDGRTGGGQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
R
C
>Mature Secondary Structure 
TEQSSAGRRFRDAVADERPLQVVGAINAYSALLAERAGFRALYVSGAGVANASYGLPDL
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
GITQLADVVEDVRRITDATRTPVLVDIDTGFGGAFSVARTVRELTRAGAAAVHIEDQVQA
CHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHH
KRCGHRPGKELVSTAEMVDRVKAAVDARTDPELVVMARTDARASEGLEAAVERAEAYVEA
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
GADMVFAEAMHSLDDIRAFTDRVPVPVLANITEFGKTPYFTVEELREAGAGLVLYPLSAF
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEHHHHHHCCCCEEEHHHHHH
RAMSRAAEAVYGAIRADGTQERVLDRMQTREELYEVLGYHDYERKLDELFGRDGRTGGGQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]