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The map label for this gene is miaB

Identifier: 121997684

GI number: 121997684

Start: 969895

End: 971319

Strand: Reverse

Name: miaB

Synonym: Hhal_0893

Alternate gene names: 121997684

Gene position: 971319-969895 (Counterclockwise)

Preceding gene: 121997685

Following gene: 121997683

Centisome position: 36.26

GC content: 70.6

Gene sequence:

>1425_bases
ATGTCCAGAAACCAGTCCCGTGAAGCGAGGCCAAACCGCGAGGTGGCGCAGACGGTGAGCGAGCGCGTCTACGTAGAGAC
CCAAGGCTGCCAGATGAACGATTACGACGCCGAGCGGCTCGTCGACGTCCTGGTCAGCCAGGCCGGCGCGCGCCGGGTCG
ATCGCCCCGAGGAGGCCGACCTGCTGCTGCTCAACACCTGCTCGGTGCGGGAGAAGGCGCAGGAGAAGGTCTTCTCGCAG
CTCGGTCGCTGGCGGCGCTACAAGCAGGACAACCCCACCGTGCTGATCGGCGTTGGTGGCTGCGTGGCCAGCCAGGAGGG
GGCCGAGATCCTGCGCCGCGCCCCCTTCGTCGACCTGGTGTTCGGGCCGCAGACCCTGCATCGCCTGCCGCAGATGCTGG
CGCGCCGGCGCTCCGGGGCCAGCGCCCAGGTCGACGTCGACTTCCCCGAGATCGAGAAGTTCGACCACCTGCCGCAGCCG
CGGGCCGAGGGGCCCACCGCCTACGTCTCGGTGATGGAGGGCTGCAGCAAGTACTGCTCCTTCTGCGTGGTGCCGTACAC
CCGCGGCGACGAGATCAGCCGGCCGGTGGCCGACGTCCTCGCCGAGGTGCGTAGCCTGGCCGAGCAGGGGGTGCGCGAGG
TCAACCTGCTGGGGCAGAACGTCAACGCCTACGCCGGCGCCCTCGACGACGGCGAGCGCGCCGACCTGGGGCTGCTCATC
GAGGCCGTGGCGCGCATCCCGGGGATCGACCGCATCCGCTTCACTACCTCGCACCCGGCGGAGTTCCACAGTGGGCTCAT
CGACGCCTACCGCGATGTGCCCGAGCTGGCCGACTTCCTGCACCTGCCGGTGCAGAGCGGCTCGGATCTGATCCTCAAGC
TGATGAAACGCGGCCACGACATCGCCGCCTACGAGGCGCTGATCGATCAGATTCGCGCCGTTCGCCCCGGGCTGGTGTTG
GCGACGGACCTGATCGTCGGCTTCCCCGGCGAGACCGAGGCCGAGTTCGAGGAGACCCTGGCCATGGTTGACCGCGTCGG
CTTCGATGGCGGCGCCTTCAGCTTCGTCTATAGCCCGCGGCCGGGGACACCGGCGGCGGAGCTGCACGACGGCGTCCCCG
AGGCCGACAAGCGCGCCTGGCTGCAGCGGCTGCAGGCGCGGCTGCACGAGCAGCAGAGTGCCGCCGCCCGGGCGCTGCTC
GGCACCAGGCAGTCGGTGCTGATCGATGGCCCGTCGCGGCGCGACCCGCGCGAGCTGAGCGGGCGCACCTCGGGCAACCG
GGTGGTGAACTTCCCCGGCGATCCGTCCTGGATCGGCCGCTTTGCCGAAGTCGAGATCACCGACGCGCGCACCCACTCGC
TCCGGGGCCGCGTCGTCTCCGTGGAGGGCCAAGCCATGCACGCAGCAGTCGGCGCAGGCGGGTAA

Upstream 100 bases:

>100_bases
CGGGGCTGCAGCGGCGGCGGTGTGGTACGCCAGCGTCGTGCTGTTGACCCCGCTGGCCGGATGATCGAGCCGTTGGGCGT
GTTGCAGTGCCAAAGCTAGA

Downstream 100 bases:

>100_bases
CGGCGTGAGCGACCGGCCGTACGCCATCGATCTCGAACTGACCCCAGCCGACAACGAGCGACTGGCGCGCTTGTGCGGGC
AGTTCGACGAGAACCTCCGT

Product: tRNA-i(6)A37 thiotransferase enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSRNQSREARPNREVAQTVSERVYVETQGCQMNDYDAERLVDVLVSQAGARRVDRPEEADLLLLNTCSVREKAQEKVFSQ
LGRWRRYKQDNPTVLIGVGGCVASQEGAEILRRAPFVDLVFGPQTLHRLPQMLARRRSGASAQVDVDFPEIEKFDHLPQP
RAEGPTAYVSVMEGCSKYCSFCVVPYTRGDEISRPVADVLAEVRSLAEQGVREVNLLGQNVNAYAGALDDGERADLGLLI
EAVARIPGIDRIRFTTSHPAEFHSGLIDAYRDVPELADFLHLPVQSGSDLILKLMKRGHDIAAYEALIDQIRAVRPGLVL
ATDLIVGFPGETEAEFEETLAMVDRVGFDGGAFSFVYSPRPGTPAAELHDGVPEADKRAWLQRLQARLHEQQSAAARALL
GTRQSVLIDGPSRRDPRELSGRTSGNRVVNFPGDPSWIGRFAEVEITDARTHSLRGRVVSVEGQAMHAAVGAGG

Sequences:

>Translated_474_residues
MSRNQSREARPNREVAQTVSERVYVETQGCQMNDYDAERLVDVLVSQAGARRVDRPEEADLLLLNTCSVREKAQEKVFSQ
LGRWRRYKQDNPTVLIGVGGCVASQEGAEILRRAPFVDLVFGPQTLHRLPQMLARRRSGASAQVDVDFPEIEKFDHLPQP
RAEGPTAYVSVMEGCSKYCSFCVVPYTRGDEISRPVADVLAEVRSLAEQGVREVNLLGQNVNAYAGALDDGERADLGLLI
EAVARIPGIDRIRFTTSHPAEFHSGLIDAYRDVPELADFLHLPVQSGSDLILKLMKRGHDIAAYEALIDQIRAVRPGLVL
ATDLIVGFPGETEAEFEETLAMVDRVGFDGGAFSFVYSPRPGTPAAELHDGVPEADKRAWLQRLQARLHEQQSAAARALL
GTRQSVLIDGPSRRDPRELSGRTSGNRVVNFPGDPSWIGRFAEVEITDARTHSLRGRVVSVEGQAMHAAVGAGG
>Mature_473_residues
SRNQSREARPNREVAQTVSERVYVETQGCQMNDYDAERLVDVLVSQAGARRVDRPEEADLLLLNTCSVREKAQEKVFSQL
GRWRRYKQDNPTVLIGVGGCVASQEGAEILRRAPFVDLVFGPQTLHRLPQMLARRRSGASAQVDVDFPEIEKFDHLPQPR
AEGPTAYVSVMEGCSKYCSFCVVPYTRGDEISRPVADVLAEVRSLAEQGVREVNLLGQNVNAYAGALDDGERADLGLLIE
AVARIPGIDRIRFTTSHPAEFHSGLIDAYRDVPELADFLHLPVQSGSDLILKLMKRGHDIAAYEALIDQIRAVRPGLVLA
TDLIVGFPGETEAEFEETLAMVDRVGFDGGAFSFVYSPRPGTPAAELHDGVPEADKRAWLQRLQARLHEQQSAAARALLG
TRQSVLIDGPSRRDPRELSGRTSGNRVVNFPGDPSWIGRFAEVEITDARTHSLRGRVVSVEGQAMHAAVGAGG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=36.8200836820084, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=36.8200836820084, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI93277076, Length=473, Percent_Identity=26.4270613107822, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=57.6233183856502, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI1787057, Length=435, Percent_Identity=29.6551724137931, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=34.8484848484849, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=316, Percent_Identity=29.1139240506329, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=34.9473684210526, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI19922432, Length=477, Percent_Identity=28.3018867924528, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_HALHL (A1WVF7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002471.1
- STRING:   A1WVF7
- GeneID:   4710109
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0893
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   A1WVF7
- BioCyc:   HHAL349124:HHAL_0893-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52067; Mature: 51936

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNQSREARPNREVAQTVSERVYVETQGCQMNDYDAERLVDVLVSQAGARRVDRPEEAD
CCCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
LLLLNTCSVREKAQEKVFSQLGRWRRYKQDNPTVLIGVGGCVASQEGAEILRRAPFVDLV
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCHHHHHHHHHCCCEEEE
FGPQTLHRLPQMLARRRSGASAQVDVDFPEIEKFDHLPQPRAEGPTAYVSVMEGCSKYCS
ECHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
FCVVPYTRGDEISRPVADVLAEVRSLAEQGVREVNLLGQNVNAYAGALDDGERADLGLLI
EEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHH
EAVARIPGIDRIRFTTSHPAEFHSGLIDAYRDVPELADFLHLPVQSGSDLILKLMKRGHD
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
IAAYEALIDQIRAVRPGLVLATDLIVGFPGETEAEFEETLAMVDRVGFDGGAFSFVYSPR
HHHHHHHHHHHHHHCCCCEEEHHHHEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
PGTPAAELHDGVPEADKRAWLQRLQARLHEQQSAAARALLGTRQSVLIDGPSRRDPRELS
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHC
GRTSGNRVVNFPGDPSWIGRFAEVEITDARTHSLRGRVVSVEGQAMHAAVGAGG
CCCCCCEEEECCCCHHHHCCEEEEEEECCHHHHCCCCEEEECCCCCEECCCCCC
>Mature Secondary Structure 
SRNQSREARPNREVAQTVSERVYVETQGCQMNDYDAERLVDVLVSQAGARRVDRPEEAD
CCCCCCCCCCCHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
LLLLNTCSVREKAQEKVFSQLGRWRRYKQDNPTVLIGVGGCVASQEGAEILRRAPFVDLV
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHCCHHHHHHHHHCCCEEEE
FGPQTLHRLPQMLARRRSGASAQVDVDFPEIEKFDHLPQPRAEGPTAYVSVMEGCSKYCS
ECHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
FCVVPYTRGDEISRPVADVLAEVRSLAEQGVREVNLLGQNVNAYAGALDDGERADLGLLI
EEEEECCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHHH
EAVARIPGIDRIRFTTSHPAEFHSGLIDAYRDVPELADFLHLPVQSGSDLILKLMKRGHD
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
IAAYEALIDQIRAVRPGLVLATDLIVGFPGETEAEFEETLAMVDRVGFDGGAFSFVYSPR
HHHHHHHHHHHHHHCCCCEEEHHHHEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
PGTPAAELHDGVPEADKRAWLQRLQARLHEQQSAAARALLGTRQSVLIDGPSRRDPRELS
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHC
GRTSGNRVVNFPGDPSWIGRFAEVEITDARTHSLRGRVVSVEGQAMHAAVGAGG
CCCCCCEEEECCCCHHHHCCEEEEEEECCHHHHCCCCEEEECCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA