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The map label for this gene is mdh

Identifier: 121997621

GI number: 121997621

Start: 912176

End: 913156

Strand: Reverse

Name: mdh

Synonym: Hhal_0830

Alternate gene names: 121997621

Gene position: 913156-912176 (Counterclockwise)

Preceding gene: 121997622

Following gene: 121997620

Centisome position: 34.09

GC content: 69.83

Gene sequence:

>981_bases
ATGAAAGCACCCGTGCGAGTCGCTGTCACCGGGGCGGCCGGCCAGATCGGCTACAGCCTGCTGTTCCGCATCGCCGCCGG
CGAGATGCTGGGCAAGGATCAACCGGTGACCCTGCAACTGCTCGAGATCCCGCAGGCCCAGGAGGCGCTGCAGGGAACGG
TGATGGAGCTTGAGGACTGCGCCTTCCCGCTGGTCGACGGCATCATCGCCACGGACAGCGCCGAAGAGGCCTTCCGAGAC
GCCGACTACGTTCTGCTCGTCGGCGCCAAGCCCCGTGGCCCGGGCATGGAGCGCAAGGACCTGCTCGAGGCCAATGCGGC
GATCTTCTCGGCCCAGGGCCAGGCGCTCAACGCCGTGGCCAAGCGCGACGTCAAGGTCCTGGCGGTGGGCAACCCGGCCA
ACACCAACGCCCTGATCACCCAGCGCAACGCGCCGGATCTGGACCCGCGGAACTTCACCGCCATGACCCGGCTCGACCAC
AACCGCGCCCTGGCGCAGCTCTCCAACAAGGTCGGCTGCCACTCCACCGAGATCCGCGGCCTGGCCGTGTGGGGCAACCA
CTCCGCCACCCAGTACCCGGACATCAGCCACTGCACCATCCAGGGCCGCCCGGCCGCCGACCAGGTCGAGCACGCCTGGG
TGAAGGACACCTTCATCCCCACCGTGCAGCAGCGCGGCGCGGCCATCATCAAGGCCCGCGGCGCCTCCAGCGCGGCGTCG
GCGGCCAGTGCCGCCATCGACCACATGCGCGACTGGGCCCTGGGCACCCCCGAGGGCGAGTGGACCAGCATGGCCGTGCC
GTCGGACGGCAGCTACGGCATCGAGGCGGGGCTGATCTACTCCTTCCCGGTGACCTGCCGGGGCGGTGACTACAAGATCG
TGTCGGGGCTGTCGGTGGACGACTTCAGCCGCGCACGCATGGACCAAACCGCCGCCGAGCTCGCCGAAGAGCGTGACGCG
GTGGCGCATCTGCTGCCCTGA

Upstream 100 bases:

>100_bases
TGCGTTGGCGGCCGCGTGGCGAGCCTCTGCTACAATCGGCGCCCCGGCCCGGGTCGTGAATCGGGCCCCGGCGGGCATCA
CAACGGACGAGGAGAGAGCC

Downstream 100 bases:

>100_bases
GCGGCAGCGGCGGACAACGCCCGGGGAGGGCCCGCATGGAACAGAACCAGGAGTCCTGGCAGGTCGCCGTGATGCCCGGC
GATGGCATCGGCCCGGAGGT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVTLQLLEIPQAQEALQGTVMELEDCAFPLVDGIIATDSAEEAFRD
ADYVLLVGAKPRGPGMERKDLLEANAAIFSAQGQALNAVAKRDVKVLAVGNPANTNALITQRNAPDLDPRNFTAMTRLDH
NRALAQLSNKVGCHSTEIRGLAVWGNHSATQYPDISHCTIQGRPAADQVEHAWVKDTFIPTVQQRGAAIIKARGASSAAS
AASAAIDHMRDWALGTPEGEWTSMAVPSDGSYGIEAGLIYSFPVTCRGGDYKIVSGLSVDDFSRARMDQTAAELAEERDA
VAHLLP

Sequences:

>Translated_326_residues
MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVTLQLLEIPQAQEALQGTVMELEDCAFPLVDGIIATDSAEEAFRD
ADYVLLVGAKPRGPGMERKDLLEANAAIFSAQGQALNAVAKRDVKVLAVGNPANTNALITQRNAPDLDPRNFTAMTRLDH
NRALAQLSNKVGCHSTEIRGLAVWGNHSATQYPDISHCTIQGRPAADQVEHAWVKDTFIPTVQQRGAAIIKARGASSAAS
AASAAIDHMRDWALGTPEGEWTSMAVPSDGSYGIEAGLIYSFPVTCRGGDYKIVSGLSVDDFSRARMDQTAAELAEERDA
VAHLLP
>Mature_326_residues
MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVTLQLLEIPQAQEALQGTVMELEDCAFPLVDGIIATDSAEEAFRD
ADYVLLVGAKPRGPGMERKDLLEANAAIFSAQGQALNAVAKRDVKVLAVGNPANTNALITQRNAPDLDPRNFTAMTRLDH
NRALAQLSNKVGCHSTEIRGLAVWGNHSATQYPDISHCTIQGRPAADQVEHAWVKDTFIPTVQQRGAAIIKARGASSAAS
AASAAIDHMRDWALGTPEGEWTSMAVPSDGSYGIEAGLIYSFPVTCRGGDYKIVSGLSVDDFSRARMDQTAAELAEERDA
VAHLLP

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=52.2658610271903, Blast_Score=358, Evalue=3e-99,
Organism=Homo sapiens, GI89886456, Length=329, Percent_Identity=24.0121580547112, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17561064, Length=325, Percent_Identity=53.8461538461538, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=51.9083969465649, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=52.9411764705882, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=50.3030303030303, Blast_Score=324, Evalue=4e-89,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_HALHL (A1WV94)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002408.1
- ProteinModelPortal:   A1WV94
- SMR:   A1WV94
- STRING:   A1WV94
- GeneID:   4711073
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0830
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   PQGRAIN
- ProtClustDB:   PRK05442
- BioCyc:   HHAL349124:HHAL_0830-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34689; Mature: 34689

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVTLQLLEIPQAQEALQGTVMELEDC
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
AFPLVDGIIATDSAEEAFRDADYVLLVGAKPRGPGMERKDLLEANAAIFSAQGQALNAVA
HHHHHCCEEECCCHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHH
KRDVKVLAVGNPANTNALITQRNAPDLDPRNFTAMTRLDHNRALAQLSNKVGCHSTEIRG
HCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCE
LAVWGNHSATQYPDISHCTIQGRPAADQVEHAWVKDTFIPTVQQRGAAIIKARGASSAAS
EEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHCCCEEEEECCCCCHHH
AASAAIDHMRDWALGTPEGEWTSMAVPSDGSYGIEAGLIYSFPVTCRGGDYKIVSGLSVD
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEECCEEEECCCEEEEECCCCC
DFSRARMDQTAAELAEERDAVAHLLP
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAPVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVTLQLLEIPQAQEALQGTVMELEDC
CCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
AFPLVDGIIATDSAEEAFRDADYVLLVGAKPRGPGMERKDLLEANAAIFSAQGQALNAVA
HHHHHCCEEECCCHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHH
KRDVKVLAVGNPANTNALITQRNAPDLDPRNFTAMTRLDHNRALAQLSNKVGCHSTEIRG
HCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCE
LAVWGNHSATQYPDISHCTIQGRPAADQVEHAWVKDTFIPTVQQRGAAIIKARGASSAAS
EEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCHHHHHCCCEEEEECCCCCHHH
AASAAIDHMRDWALGTPEGEWTSMAVPSDGSYGIEAGLIYSFPVTCRGGDYKIVSGLSVD
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEEECCEEEECCCEEEEECCCCC
DFSRARMDQTAAELAEERDAVAHLLP
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA