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The map label for this gene is rlmB [H]

Identifier: 121997455

GI number: 121997455

Start: 738921

End: 739736

Strand: Reverse

Name: rlmB [H]

Synonym: Hhal_0658

Alternate gene names: 121997455

Gene position: 739736-738921 (Counterclockwise)

Preceding gene: 121997456

Following gene: 121997454

Centisome position: 27.62

GC content: 73.28

Gene sequence:

>816_bases
ATGGCGCAGAACAAGCGACGCCGGGGGCGGGGCGCCAGGCCGGCTCCCGCCGACCCCGCGGATCCCGCCGCGCTGGTCTA
CGGTCAGCACGCTGCGCGTGAGGCCGCCGCCTACGACCCGGCCGGCGTGGAGGCGGTCTGGGTGGAGCACGGCCGGCGGG
ACGCCCGGCTGGAGCGCCTGCTCGACAAACTCGGTCGTCAGGGCGTGACCGTCGAACGGGTCCATCGGCGGGTTCTCGAT
CAGATGGCCGAGGGCGGTAACCACCAGGGCATCGTCCTGCGCTACAGCGGGACCCCGGCCCGCGGCGAGGGCGAGCTGGC
GGACCTGGTGGACGCTGAGCCCGAACCGCTGCTGCTGGTCCTGGATCGGGTGCAGGATCCGCACAACCTCGGTGCGTGCA
TGCGCAGTGCCGCCGCGGCCGGTGCCCACGGGGTGGTGGCCCCCCGGGATCGGGCGGCAGCGCTGAGTCCGGCGGTTCAC
AAGACAGCTGCCGGGGCGGTGCAACGCATCCCGTTCTTCCAGGTGACGAATCTCGCCCGCACCCTGCAGCAGCTGCGCGA
CGCCGGGGTCCACGTGGTGGGCGCCGCCGGCGAGGCCGAGAAGAGCTGCTACGAGGTGGATCTGCGCGGGCCGCTGGCCC
TGGTCCTCGGCGGCGAGGGTGAGGGCCTGCGGCGGTTGACCCGGGAGCGCTGCGACGAACGGGTCGCGATCCCGATGCCG
GGGTCGATGGAGAGCCTCAATGTCTCCGTGGCGGCCGGCGTTCTGCTGTTCGAGGCGGTCCGCCAGCGCTTTCCACAGGG
TGGCGATCTCGGGTAG

Upstream 100 bases:

>100_bases
AAGCGCAGGATGGCGGCGCCAGCAAGGGGCGGCGCCGAAGCCGGCGCTCCGGTCGTCGGCGCAGCAGCAGTAGCGCCGGC
GGCGACAGCAGCTAACGACC

Downstream 100 bases:

>100_bases
ACTTGCCCGCTTAACCCAAGGTGCAACCTTGCCTCACATCCGTGGGGGTGGAGGCTGTTTGAGCCTGTGGCTCGAATAAC
CGTAAGGAGTAACGGATGCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAQNKRRRGRGARPAPADPADPAALVYGQHAAREAAAYDPAGVEAVWVEHGRRDARLERLLDKLGRQGVTVERVHRRVLD
QMAEGGNHQGIVLRYSGTPARGEGELADLVDAEPEPLLLVLDRVQDPHNLGACMRSAAAAGAHGVVAPRDRAAALSPAVH
KTAAGAVQRIPFFQVTNLARTLQQLRDAGVHVVGAAGEAEKSCYEVDLRGPLALVLGGEGEGLRRLTRERCDERVAIPMP
GSMESLNVSVAAGVLLFEAVRQRFPQGGDLG

Sequences:

>Translated_271_residues
MAQNKRRRGRGARPAPADPADPAALVYGQHAAREAAAYDPAGVEAVWVEHGRRDARLERLLDKLGRQGVTVERVHRRVLD
QMAEGGNHQGIVLRYSGTPARGEGELADLVDAEPEPLLLVLDRVQDPHNLGACMRSAAAAGAHGVVAPRDRAAALSPAVH
KTAAGAVQRIPFFQVTNLARTLQQLRDAGVHVVGAAGEAEKSCYEVDLRGPLALVLGGEGEGLRRLTRERCDERVAIPMP
GSMESLNVSVAAGVLLFEAVRQRFPQGGDLG
>Mature_270_residues
AQNKRRRGRGARPAPADPADPAALVYGQHAAREAAAYDPAGVEAVWVEHGRRDARLERLLDKLGRQGVTVERVHRRVLDQ
MAEGGNHQGIVLRYSGTPARGEGELADLVDAEPEPLLLVLDRVQDPHNLGACMRSAAAAGAHGVVAPRDRAAALSPAVHK
TAAGAVQRIPFFQVTNLARTLQQLRDAGVHVVGAAGEAEKSCYEVDLRGPLALVLGGEGEGLRRLTRERCDERVAIPMPG
SMESLNVSVAAGVLLFEAVRQRFPQGGDLG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=221, Percent_Identity=36.1990950226244, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1790623, Length=239, Percent_Identity=48.5355648535565, Blast_Score=237, Evalue=7e-64,
Organism=Escherichia coli, GI1788935, Length=148, Percent_Identity=39.1891891891892, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790083, Length=150, Percent_Identity=30, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=249, Percent_Identity=28.9156626506024, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324775, Length=284, Percent_Identity=28.169014084507, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28873; Mature: 28742

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQNKRRRGRGARPAPADPADPAALVYGQHAAREAAAYDPAGVEAVWVEHGRRDARLERL
CCCCCCCCCCCCCCCCCCCCCCHHEECCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
LDKLGRQGVTVERVHRRVLDQMAEGGNHQGIVLRYSGTPARGEGELADLVDAEPEPLLLV
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEE
LDRVQDPHNLGACMRSAAAAGAHGVVAPRDRAAALSPAVHKTAAGAVQRIPFFQVTNLAR
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHH
TLQQLRDAGVHVVGAAGEAEKSCYEVDLRGPLALVLGGEGEGLRRLTRERCDERVAIPMP
HHHHHHHCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCEEECCCC
GSMESLNVSVAAGVLLFEAVRQRFPQGGDLG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AQNKRRRGRGARPAPADPADPAALVYGQHAAREAAAYDPAGVEAVWVEHGRRDARLERL
CCCCCCCCCCCCCCCCCCCCCHHEECCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHH
LDKLGRQGVTVERVHRRVLDQMAEGGNHQGIVLRYSGTPARGEGELADLVDAEPEPLLLV
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEE
LDRVQDPHNLGACMRSAAAAGAHGVVAPRDRAAALSPAVHKTAAGAVQRIPFFQVTNLAR
EECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHCCHHHHHHHHH
TLQQLRDAGVHVVGAAGEAEKSCYEVDLRGPLALVLGGEGEGLRRLTRERCDERVAIPMP
HHHHHHHCCEEEEECCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCEEECCCC
GSMESLNVSVAAGVLLFEAVRQRFPQGGDLG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]