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The map label for this gene is rluB [H]

Identifier: 121997382

GI number: 121997382

Start: 658219

End: 659055

Strand: Reverse

Name: rluB [H]

Synonym: Hhal_0581

Alternate gene names: 121997382

Gene position: 659055-658219 (Counterclockwise)

Preceding gene: 121997383

Following gene: 121997374

Centisome position: 24.61

GC content: 71.09

Gene sequence:

>837_bases
GTGACGAGTGACGGTGGGAATCAACCCGCAGGGGAGAAACTGCAGAAGGTGCTGGCGCGTGCCGGGCTCGGCTCGCGCCG
CGAGATGGAGGCGGCCATCCAGGCCGGACGCGTGCGCATCGAGGGGCGTACGGCCAAGCTCGGCGACCGCGCACGGCCCC
GCGATCACGTGGAGCTGGACGGCCGCACGGTGCAGCGGGTCCACCGCGAGCCGCCGCGGCGGGTCCTGCTCTATAACAAG
CCCGAGGGCGAGGTGACCACCCGCTCCGATCCGGAAGGTCGGTCGACGGTCTTTGAGCGGCTGCCGCGCACGCCCGGGCG
GTGGATCGCCGTCGGGCGGCTGGATATCAACAGCCAGGGCCTGCTCCTGTTCACCAATGATGGCGAGTTGGCCAACGCCC
TGATGCACCCCTCCACCGGTGTCGAGCGCGAGTACGCCTGCCGGATCCACGGTGAAGTCACCGAGGAGATGGTCCATCGG
CTGGTGGACGGCGTCGAGCTGGAGGACGGTCCCGCCCACTTCGAGGTCGTGGAGTCGGGGATGCCGGTGGACGAACCCCA
GGAGGGCACCAACGCCTGGTACCACGTCATTGTCACCGAGGGCCGCCGCCGGGAGGTGCGCCGGCTATGGGAGTCGCAGG
GGGTCACGGTCAGCCGCCTGATCCGCGTGCGCTACGGGCCCATGGTGATGCCACGGGATCTGCGCCGCGGCCAGTTGCGC
GAGCTGGATGAGGCCGGCAAGCGCGAGCTCTATGAGCACGTCGGTCTGACCCCGCCGGAGCCGGTCAGGCGGCCGGCCCC
GCAGCGTCGCCGGCGGCGTGGGCGCAAGCGGCGTTGA

Upstream 100 bases:

>100_bases
CCGGACCAGGCGCATCTGGAGCTGGATCTGCGTCTGCCCGACGAGGGCGACGAAAGCGACGAGGATCCGGCAACCAGCGA
TGAACAACAGGGGGCGACCC

Downstream 100 bases:

>100_bases
GCGGGCAGTCCGGGCAGCGCGGCGTGGGGCGACAGGTGTCCTTACCCAGAGCGACGATCTGGGCGTGCAGCTCGTTGAAG
GCCGCGGTGTTCGGCCCCAG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTSDGGNQPAGEKLQKVLARAGLGSRREMEAAIQAGRVRIEGRTAKLGDRARPRDHVELDGRTVQRVHREPPRRVLLYNK
PEGEVTTRSDPEGRSTVFERLPRTPGRWIAVGRLDINSQGLLLFTNDGELANALMHPSTGVEREYACRIHGEVTEEMVHR
LVDGVELEDGPAHFEVVESGMPVDEPQEGTNAWYHVIVTEGRRREVRRLWESQGVTVSRLIRVRYGPMVMPRDLRRGQLR
ELDEAGKRELYEHVGLTPPEPVRRPAPQRRRRRGRKRR

Sequences:

>Translated_278_residues
MTSDGGNQPAGEKLQKVLARAGLGSRREMEAAIQAGRVRIEGRTAKLGDRARPRDHVELDGRTVQRVHREPPRRVLLYNK
PEGEVTTRSDPEGRSTVFERLPRTPGRWIAVGRLDINSQGLLLFTNDGELANALMHPSTGVEREYACRIHGEVTEEMVHR
LVDGVELEDGPAHFEVVESGMPVDEPQEGTNAWYHVIVTEGRRREVRRLWESQGVTVSRLIRVRYGPMVMPRDLRRGQLR
ELDEAGKRELYEHVGLTPPEPVRRPAPQRRRRRGRKRR
>Mature_277_residues
TSDGGNQPAGEKLQKVLARAGLGSRREMEAAIQAGRVRIEGRTAKLGDRARPRDHVELDGRTVQRVHREPPRRVLLYNKP
EGEVTTRSDPEGRSTVFERLPRTPGRWIAVGRLDINSQGLLLFTNDGELANALMHPSTGVEREYACRIHGEVTEEMVHRL
VDGVELEDGPAHFEVVESGMPVDEPQEGTNAWYHVIVTEGRRREVRRLWESQGVTVSRLIRVRYGPMVMPRDLRRGQLRE
LDEAGKRELYEHVGLTPPEPVRRPAPQRRRRRGRKRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=266, Percent_Identity=57.1428571428571, Blast_Score=263, Evalue=8e-72,
Organism=Escherichia coli, GI1790453, Length=213, Percent_Identity=30.5164319248826, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=33.160621761658, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=27.6150627615063, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 31751; Mature: 31619

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDGGNQPAGEKLQKVLARAGLGSRREMEAAIQAGRVRIEGRTAKLGDRARPRDHVELD
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCEEEC
GRTVQRVHREPPRRVLLYNKPEGEVTTRSDPEGRSTVFERLPRTPGRWIAVGRLDINSQG
CHHHHHHHCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCC
LLLFTNDGELANALMHPSTGVEREYACRIHGEVTEEMVHRLVDGVELEDGPAHFEVVESG
EEEEECCCHHHHHHHCCCCCCCCHHEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
MPVDEPQEGTNAWYHVIVTEGRRREVRRLWESQGVTVSRLIRVRYGPMVMPRDLRRGQLR
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHCCHHH
ELDEAGKRELYEHVGLTPPEPVRRPAPQRRRRRGRKRR
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHCCCCCC
>Mature Secondary Structure 
TSDGGNQPAGEKLQKVLARAGLGSRREMEAAIQAGRVRIEGRTAKLGDRARPRDHVELD
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCEEEC
GRTVQRVHREPPRRVLLYNKPEGEVTTRSDPEGRSTVFERLPRTPGRWIAVGRLDINSQG
CHHHHHHHCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCC
LLLFTNDGELANALMHPSTGVEREYACRIHGEVTEEMVHRLVDGVELEDGPAHFEVVESG
EEEEECCCHHHHHHHCCCCCCCCHHEEEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
MPVDEPQEGTNAWYHVIVTEGRRREVRRLWESQGVTVSRLIRVRYGPMVMPRDLRRGQLR
CCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHCCHHH
ELDEAGKRELYEHVGLTPPEPVRRPAPQRRRRRGRKRR
HHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]