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The map label for this gene is btuB [H]

Identifier: 121611677

GI number: 121611677

Start: 5267591

End: 5269465

Strand: Direct

Name: btuB [H]

Synonym: Veis_4773

Alternate gene names: 121611677

Gene position: 5267591-5269465 (Clockwise)

Preceding gene: 121611674

Following gene: 121611678

Centisome position: 94.63

GC content: 70.72

Gene sequence:

>1875_bases
ATGAATATCCGTTCCCTGCCTGCGTGCCTGCGCCCCTGTGCGCTGGCCGTTCTTGCCGTCGGCGCCGGCCAGATGCTCCA
TGCCCAAGGACTGCCCGACACACCAGGGCTTGCACCGGGGCTGGCCGCGCCCGCGCTGCAAGATACCGTGGTCAGCGCCA
CGCGCACCGAGCAGCCCTTGTCCGACCTGGTGGCCGACCTGTCCATCATCGAGCGCGAAACCATCGAGCGCAGCGGTTTG
ACCGGTGTGGCCGACCTGCTGGCGCGCCTGCCTGGCGTGGAGATGGCGCGCAACGGCGGAATCGGCGGTGCCGGCAGCGT
TTTCCTGCGCGCTGCCGACTCGCGCCACACGGCGGTTTTCATCGATGGCGTGCGGGTGGACTCGCAAACCACGGGCGGGG
CGCAATGGGAGCTGATCCCGCTGGCGCAGATCGACCGCATCGAAGTGCTGCGCGGCCCGGCCGCCGCTGTCTATGGCTCG
GACGCGATCGGCGGGGTGATACAGCTTTTCACCCGCAAAGGGGAAGGCCCGGCCAAGCCGTATGTGGGCCTGGGCCTGGG
CAACCTGGGCAGCCGCATGTTTGACACCGGCATCAGCGGCAGCGCCGGGCAGGACGGCGCGTTCGGCTACACGCTCGGGC
TGGCGCGCGACAGCAGCGCCGGCTTCGACGCCAAGACCGGCGTCGCCCACAACCCGGACCGCGACGACTACCGCAGCACC
TCCGGCCACGCCCGGCTGGGTTGGCGCATCGATGCCCGGCACCATGTCGATGCCACGCTGCTGGCCAACCGCATGGATGC
GGGCTATGACGAGTACAGCGCCGCCCCCCCCGTGGACGACCGCAGCCTGGGCCGCCTGCGCACGGCCGGGCTGACCTGGG
CGGCGCAATGGTCCGGGGCTTACGGCACGCGCCTGTCGGTCAGCGATGCGCTCAGCCGTTACGCGAGCGCGCCAACCCCC
TATCTGGCCGAGACCAGGCTGCGCGGCTACCTCTGGCAAAACGATTACCGCCGGGGCGCGCACCGCTTCAGCGCCGCGCT
GGAGCGCCGTGAAGACGCGCTGCAAAACGGCCCCATCGACCGCAGCCGCGCGCAGGATGCGCTGGCGCTGGGCTATGGCT
TGAGCACCGGGCGGCACACCGTGCAACTGAACCTGCGCCACGATGGCGACAGCGACTTCGGCAGCAGCAGCACCGGCAGC
GCCGCCTATGGCTACGCCCTGGACGCCCACTGGCGCGCCACCGCCTCGCTGGGCAGCGCCTTTCGGGCGCCCACCCTGTA
CCAGCGCTTTAGCGCCTACGGCCTGCCCACCTTGCAGCCCGAGCGCAGCCGCAACATCGAACTGGGTCTGCGCTACGGCC
AGGGCCGCAGCAGCTTCTCGCTGCTGGCCTACCGCAACCGGCTGCGCAACCTGATCGCGTTCGCCGAGGCGGGCCCCTGT
GCCTCGTCCCATGGCTGCTACGCCAACAGGACGCGCGCGCAATACCAGGGGCTGACCCTGGCCGGCGCCTTGCCGCTGTC
GGGCTGGCAACTGCACGGCGCGCTGGACTTGCAAAATCCGCGCGACCCGGATACTGGCCGGCAACTGGCGCGCCGGGCCA
GACGCCATGCCACGCTGGGCGCCGACACGCGCCTGGCCGACTGGAGCGTGGGCGCCGAGGTGCAGGCCTCGGGCCGGCGC
TTCGACAACGCCGCCAACACCAAGGTGCTGGGGGGCTATGCGCTGCTGAACCTGTACGCCAGCACCCGCATCGCCCGCGA
CTGCACGCTGCTGGCACGCATCGACAACCTGGCCGACAAAGACTACCCACTGGCCCGCAGCTACGCCACGCCCGGCCGCA
CGCTGTATGTCGGTGTGAAATGGGCCGCGCAATGA

Upstream 100 bases:

>100_bases
GCGCCCCGGTGCATTCGTGCGCCGGGTGTTCGGCAAGCCGTATCACCCAAATGCTGGCGGGGAAGCCGGCGCGGGTCGTT
TGTTGTCTGTGACGATTGCC

Downstream 100 bases:

>100_bases
AGATCACGACAGCGCCGCGCTGCCCGCCCAAAGCGCGCAGCCTGGCTGGGCGCGGCCCGGCGCGGCGGCCGATGCAAGTG
CGCGCGCTGCCCGCGCTGCC

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MNIRSLPACLRPCALAVLAVGAGQMLHAQGLPDTPGLAPGLAAPALQDTVVSATRTEQPLSDLVADLSIIERETIERSGL
TGVADLLARLPGVEMARNGGIGGAGSVFLRAADSRHTAVFIDGVRVDSQTTGGAQWELIPLAQIDRIEVLRGPAAAVYGS
DAIGGVIQLFTRKGEGPAKPYVGLGLGNLGSRMFDTGISGSAGQDGAFGYTLGLARDSSAGFDAKTGVAHNPDRDDYRST
SGHARLGWRIDARHHVDATLLANRMDAGYDEYSAAPPVDDRSLGRLRTAGLTWAAQWSGAYGTRLSVSDALSRYASAPTP
YLAETRLRGYLWQNDYRRGAHRFSAALERREDALQNGPIDRSRAQDALALGYGLSTGRHTVQLNLRHDGDSDFGSSSTGS
AAYGYALDAHWRATASLGSAFRAPTLYQRFSAYGLPTLQPERSRNIELGLRYGQGRSSFSLLAYRNRLRNLIAFAEAGPC
ASSHGCYANRTRAQYQGLTLAGALPLSGWQLHGALDLQNPRDPDTGRQLARRARRHATLGADTRLADWSVGAEVQASGRR
FDNAANTKVLGGYALLNLYASTRIARDCTLLARIDNLADKDYPLARSYATPGRTLYVGVKWAAQ

Sequences:

>Translated_624_residues
MNIRSLPACLRPCALAVLAVGAGQMLHAQGLPDTPGLAPGLAAPALQDTVVSATRTEQPLSDLVADLSIIERETIERSGL
TGVADLLARLPGVEMARNGGIGGAGSVFLRAADSRHTAVFIDGVRVDSQTTGGAQWELIPLAQIDRIEVLRGPAAAVYGS
DAIGGVIQLFTRKGEGPAKPYVGLGLGNLGSRMFDTGISGSAGQDGAFGYTLGLARDSSAGFDAKTGVAHNPDRDDYRST
SGHARLGWRIDARHHVDATLLANRMDAGYDEYSAAPPVDDRSLGRLRTAGLTWAAQWSGAYGTRLSVSDALSRYASAPTP
YLAETRLRGYLWQNDYRRGAHRFSAALERREDALQNGPIDRSRAQDALALGYGLSTGRHTVQLNLRHDGDSDFGSSSTGS
AAYGYALDAHWRATASLGSAFRAPTLYQRFSAYGLPTLQPERSRNIELGLRYGQGRSSFSLLAYRNRLRNLIAFAEAGPC
ASSHGCYANRTRAQYQGLTLAGALPLSGWQLHGALDLQNPRDPDTGRQLARRARRHATLGADTRLADWSVGAEVQASGRR
FDNAANTKVLGGYALLNLYASTRIARDCTLLARIDNLADKDYPLARSYATPGRTLYVGVKWAAQ
>Mature_624_residues
MNIRSLPACLRPCALAVLAVGAGQMLHAQGLPDTPGLAPGLAAPALQDTVVSATRTEQPLSDLVADLSIIERETIERSGL
TGVADLLARLPGVEMARNGGIGGAGSVFLRAADSRHTAVFIDGVRVDSQTTGGAQWELIPLAQIDRIEVLRGPAAAVYGS
DAIGGVIQLFTRKGEGPAKPYVGLGLGNLGSRMFDTGISGSAGQDGAFGYTLGLARDSSAGFDAKTGVAHNPDRDDYRST
SGHARLGWRIDARHHVDATLLANRMDAGYDEYSAAPPVDDRSLGRLRTAGLTWAAQWSGAYGTRLSVSDALSRYASAPTP
YLAETRLRGYLWQNDYRRGAHRFSAALERREDALQNGPIDRSRAQDALALGYGLSTGRHTVQLNLRHDGDSDFGSSSTGS
AAYGYALDAHWRATASLGSAFRAPTLYQRFSAYGLPTLQPERSRNIELGLRYGQGRSSFSLLAYRNRLRNLIAFAEAGPC
ASSHGCYANRTRAQYQGLTLAGALPLSGWQLHGALDLQNPRDPDTGRQLARRARRHATLGADTRLADWSVGAEVQASGRR
FDNAANTKVLGGYALLNLYASTRIARDCTLLARIDNLADKDYPLARSYATPGRTLYVGVKWAAQ

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=602, Percent_Identity=28.9036544850498, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788478, Length=655, Percent_Identity=24.5801526717557, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786798, Length=150, Percent_Identity=32.6666666666667, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787723, Length=251, Percent_Identity=26.2948207171315, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 66696; Mature: 66696

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRSLPACLRPCALAVLAVGAGQMLHAQGLPDTPGLAPGLAAPALQDTVVSATRTEQPL
CCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
SDLVADLSIIERETIERSGLTGVADLLARLPGVEMARNGGIGGAGSVFLRAADSRHTAVF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCCCCEEEE
IDGVRVDSQTTGGAQWELIPLAQIDRIEVLRGPAAAVYGSDAIGGVIQLFTRKGEGPAKP
EEEEEECCCCCCCCEEEEEECCCCCHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCC
YVGLGLGNLGSRMFDTGISGSAGQDGAFGYTLGLARDSSAGFDAKTGVAHNPDRDDYRST
EEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCC
SGHARLGWRIDARHHVDATLLANRMDAGYDEYSAAPPVDDRSLGRLRTAGLTWAAQWSGA
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCC
YGTRLSVSDALSRYASAPTPYLAETRLRGYLWQNDYRRGAHRFSAALERREDALQNGPID
CCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSRAQDALALGYGLSTGRHTVQLNLRHDGDSDFGSSSTGSAAYGYALDAHWRATASLGSA
HHHHHHHHHEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCEECEEEECCHHHHHHHHHH
FRAPTLYQRFSAYGLPTLQPERSRNIELGLRYGQGRSSFSLLAYRNRLRNLIAFAEAGPC
HCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
ASSHGCYANRTRAQYQGLTLAGALPLSGWQLHGALDLQNPRDPDTGRQLARRARRHATLG
CCCCCCCCCCCHHHHCCEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
ADTRLADWSVGAEVQASGRRFDNAANTKVLGGYALLNLYASTRIARDCTLLARIDNLADK
CCCEECCCCCCCEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DYPLARSYATPGRTLYVGVKWAAQ
CCCCHHCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MNIRSLPACLRPCALAVLAVGAGQMLHAQGLPDTPGLAPGLAAPALQDTVVSATRTEQPL
CCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
SDLVADLSIIERETIERSGLTGVADLLARLPGVEMARNGGIGGAGSVFLRAADSRHTAVF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCCCCEEEE
IDGVRVDSQTTGGAQWELIPLAQIDRIEVLRGPAAAVYGSDAIGGVIQLFTRKGEGPAKP
EEEEEECCCCCCCCEEEEEECCCCCHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCCC
YVGLGLGNLGSRMFDTGISGSAGQDGAFGYTLGLARDSSAGFDAKTGVAHNPDRDDYRST
EEECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHCCC
SGHARLGWRIDARHHVDATLLANRMDAGYDEYSAAPPVDDRSLGRLRTAGLTWAAQWSGA
CCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCEEEEECCCC
YGTRLSVSDALSRYASAPTPYLAETRLRGYLWQNDYRRGAHRFSAALERREDALQNGPID
CCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSRAQDALALGYGLSTGRHTVQLNLRHDGDSDFGSSSTGSAAYGYALDAHWRATASLGSA
HHHHHHHHHEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCEECEEEECCHHHHHHHHHH
FRAPTLYQRFSAYGLPTLQPERSRNIELGLRYGQGRSSFSLLAYRNRLRNLIAFAEAGPC
HCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
ASSHGCYANRTRAQYQGLTLAGALPLSGWQLHGALDLQNPRDPDTGRQLARRARRHATLG
CCCCCCCCCCCHHHHCCEEEECCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
ADTRLADWSVGAEVQASGRRFDNAANTKVLGGYALLNLYASTRIARDCTLLARIDNLADK
CCCEECCCCCCCEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DYPLARSYATPGRTLYVGVKWAAQ
CCCCHHCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA