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The map label for this gene is leuC [H]

Identifier: 121611561

GI number: 121611561

Start: 5144831

End: 5146237

Strand: Direct

Name: leuC [H]

Synonym: Veis_4653

Alternate gene names: 121611561

Gene position: 5144831-5146237 (Clockwise)

Preceding gene: 121611560

Following gene: 121611562

Centisome position: 92.42

GC content: 63.54

Gene sequence:

>1407_bases
ATGAACCAGCGCACCTTGTTCGACAAGATCTGGGACGAGCACGTGGTGGATGTGGGGGCGGACGGAACAACCTTGCTCTA
CGTGGATCGTCATTTTCTGCACGAAGTGACGAGTCCGCAGGCGTTCCGCGGCCTGCAGCTAGCGGGCCGCAGGCCATGGC
GCAGCGATGCCAACCTTGCCATGCCGGACCACAACGTTCCCACGAAGGATCGCGCTGCGCATCTCGACGATGCCCTTGCG
CGTGCGCAGATCGAAGCACTGGATCGGAACTGCGCCGAATTCGCAGTGCCGTTGTTCCGGTTGTCCGATCCCAGGCAGGG
CATCGTGCACGTGTCCGGACCCGAACAAGGCGCGTCATTGCCGGGGAGCGTGATCGTTTGCGGCGACAGCCATACCAGCA
CGCATGGCGCGCTGGCCACCATCGCCCACGGCATCGGAACCTCCGAAGTGGAGCATGTGCTGGCGACGCAGACCCTGCTG
GCACGCAAGCCAGCCCGCATGCGGGTCAACTTCCACGGTGTTGCGGGAGCGGGTGTCAGCGCCAAGGACATGGCGCTGGC
CATGATCGCGAAGGTCGGCGCCGCTGGCGCGCAAGGCTACGCCATCGAGTACGCCGGAGAAGCCGTTCGCTGCTTGTCTG
TAGAAGCCCGAATGACACTGTGCAACATGTCAATCGAGGCTGGTGCCCGGTGTGGCATGGTCGCTGTCGACGACAAGACA
ATCGAGTATCTGCGAGGGCGTCCGATGGCGCCTCGTGGCGAAAGCTGGGATCTGGCCGTGCGCACCTGGCGTGCCTTGGT
CTCGGACCATGACGCGCAGTTCGATGTCGAGATCGAGATCGACGTGGGCATGCTTGGCCCTCTCGTGACCTGGGGGACAT
CGCCGGACATGGTGATCTCCGTCGACGAAAAAATCCCTGACCCCGCGGCGGAGAAGGACGGTACACGACGGGCGGGGATG
GAGCGGGCATTGCGCTACATGGACCTTGCCCCAGGCACGCCGATCACCGATGTTCGCCTGGACAGAATATTCATCGGCTC
GTGCACGAACTCCCGCCTGGAAGACTTGCGGGCCGCTGCCGAGGTTATCCGGGGAAAGCACGTTGCGGCCTCGATCCGCC
AGGCGCTGGTGGTCCCAGGGTCGGGGCTCGTGAAGCAACAAGCCGAGAACGAGGGCCTGCACCAGCTCTTCATCGATGCA
GGCTTTGAATGGCGCGATGCCGGATGCTCGATGTGCGTGGGCATGAATGCAGATCACCTGCTGTCCGGAGAGCGCTGCGC
ATCGACCTCCAACCGAAATTTTGAGGATCGGCAGGGTGTCGGAGGTCGGACTCACCTGGTTAGTCCGGCGATGGCAGCCG
CGGCAGCGATTGCGGGCCACTTCGTCGACATCCGGCGATCGTCATGA

Upstream 100 bases:

>100_bases
TGCCTCGACTGGCTCGCAGAGCGAGGACTGCACGATTGATGCGTGAACCGGCCCTCGAACGGTCGAGGGCCGCCACCGTT
CTACAAAGAGCAGATTCAAG

Downstream 100 bases:

>100_bases
GTCCTGCTCCCGCGAATTCCGAAGCCATGTCAGTGGTCCTTCTGTCGTTTGCGAAGTGGTTCGCCTGGCGACATCGCTGA
CGTTTTTATTCGATTCCTTC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNQRTLFDKIWDEHVVDVGADGTTLLYVDRHFLHEVTSPQAFRGLQLAGRRPWRSDANLAMPDHNVPTKDRAAHLDDALA
RAQIEALDRNCAEFAVPLFRLSDPRQGIVHVSGPEQGASLPGSVIVCGDSHTSTHGALATIAHGIGTSEVEHVLATQTLL
ARKPARMRVNFHGVAGAGVSAKDMALAMIAKVGAAGAQGYAIEYAGEAVRCLSVEARMTLCNMSIEAGARCGMVAVDDKT
IEYLRGRPMAPRGESWDLAVRTWRALVSDHDAQFDVEIEIDVGMLGPLVTWGTSPDMVISVDEKIPDPAAEKDGTRRAGM
ERALRYMDLAPGTPITDVRLDRIFIGSCTNSRLEDLRAAAEVIRGKHVAASIRQALVVPGSGLVKQQAENEGLHQLFIDA
GFEWRDAGCSMCVGMNADHLLSGERCASTSNRNFEDRQGVGGRTHLVSPAMAAAAAIAGHFVDIRRSS

Sequences:

>Translated_468_residues
MNQRTLFDKIWDEHVVDVGADGTTLLYVDRHFLHEVTSPQAFRGLQLAGRRPWRSDANLAMPDHNVPTKDRAAHLDDALA
RAQIEALDRNCAEFAVPLFRLSDPRQGIVHVSGPEQGASLPGSVIVCGDSHTSTHGALATIAHGIGTSEVEHVLATQTLL
ARKPARMRVNFHGVAGAGVSAKDMALAMIAKVGAAGAQGYAIEYAGEAVRCLSVEARMTLCNMSIEAGARCGMVAVDDKT
IEYLRGRPMAPRGESWDLAVRTWRALVSDHDAQFDVEIEIDVGMLGPLVTWGTSPDMVISVDEKIPDPAAEKDGTRRAGM
ERALRYMDLAPGTPITDVRLDRIFIGSCTNSRLEDLRAAAEVIRGKHVAASIRQALVVPGSGLVKQQAENEGLHQLFIDA
GFEWRDAGCSMCVGMNADHLLSGERCASTSNRNFEDRQGVGGRTHLVSPAMAAAAAIAGHFVDIRRSS
>Mature_468_residues
MNQRTLFDKIWDEHVVDVGADGTTLLYVDRHFLHEVTSPQAFRGLQLAGRRPWRSDANLAMPDHNVPTKDRAAHLDDALA
RAQIEALDRNCAEFAVPLFRLSDPRQGIVHVSGPEQGASLPGSVIVCGDSHTSTHGALATIAHGIGTSEVEHVLATQTLL
ARKPARMRVNFHGVAGAGVSAKDMALAMIAKVGAAGAQGYAIEYAGEAVRCLSVEARMTLCNMSIEAGARCGMVAVDDKT
IEYLRGRPMAPRGESWDLAVRTWRALVSDHDAQFDVEIEIDVGMLGPLVTWGTSPDMVISVDEKIPDPAAEKDGTRRAGM
ERALRYMDLAPGTPITDVRLDRIFIGSCTNSRLEDLRAAAEVIRGKHVAASIRQALVVPGSGLVKQQAENEGLHQLFIDA
GFEWRDAGCSMCVGMNADHLLSGERCASTSNRNFEDRQGVGGRTHLVSPAMAAAAAIAGHFVDIRRSS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=375, Percent_Identity=28.5333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI8659555, Length=435, Percent_Identity=24.8275862068966, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=57.2354211663067, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI87081781, Length=324, Percent_Identity=25.3086419753086, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=23.8888888888889, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=421, Percent_Identity=27.3159144893112, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564738, Length=421, Percent_Identity=27.3159144893112, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=27.8688524590164, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=459, Percent_Identity=25.9259259259259, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=55.863539445629, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6323335, Length=364, Percent_Identity=29.3956043956044, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320440, Length=354, Percent_Identity=26.5536723163842, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=366, Percent_Identity=27.3224043715847, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571643, Length=416, Percent_Identity=26.9230769230769, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161076999, Length=416, Percent_Identity=26.2019230769231, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281365315, Length=416, Percent_Identity=26.2019230769231, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17864292, Length=416, Percent_Identity=26.2019230769231, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50378; Mature: 50378

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRTLFDKIWDEHVVDVGADGTTLLYVDRHFLHEVTSPQAFRGLQLAGRRPWRSDANLA
CCCCHHHHHHHHHHEEECCCCCCEEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCCCC
MPDHNVPTKDRAAHLDDALARAQIEALDRNCAEFAVPLFRLSDPRQGIVHVSGPEQGASL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
PGSVIVCGDSHTSTHGALATIAHGIGTSEVEHVLATQTLLARKPARMRVNFHGVAGAGVS
CCCEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
AKDMALAMIAKVGAAGAQGYAIEYAGEAVRCLSVEARMTLCNMSIEAGARCGMVAVDDKT
HHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHEEEECCHHCCCCCCEEEECCHH
IEYLRGRPMAPRGESWDLAVRTWRALVSDHDAQFDVEIEIDVGMLGPLVTWGTSPDMVIS
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEECCCCCCEEEE
VDEKIPDPAAEKDGTRRAGMERALRYMDLAPGTPITDVRLDRIFIGSCTNSRLEDLRAAA
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHH
EVIRGKHVAASIRQALVVPGSGLVKQQAENEGLHQLFIDAGFEWRDAGCSMCVGMNADHL
HHHCCHHHHHHHHHHEECCCCCHHHHHHHCCCCCEEHHHCCCCCCCCCCCEEECCCHHHH
LSGERCASTSNRNFEDRQGVGGRTHLVSPAMAAAAAIAGHFVDIRRSS
CCCCHHCCCCCCCCHHHCCCCCCCEECCHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure
MNQRTLFDKIWDEHVVDVGADGTTLLYVDRHFLHEVTSPQAFRGLQLAGRRPWRSDANLA
CCCCHHHHHHHHHHEEECCCCCCEEEEECHHHHHHCCCCHHHCCHHHCCCCCCCCCCCCC
MPDHNVPTKDRAAHLDDALARAQIEALDRNCAEFAVPLFRLSDPRQGIVHVSGPEQGASL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
PGSVIVCGDSHTSTHGALATIAHGIGTSEVEHVLATQTLLARKPARMRVNFHGVAGAGVS
CCCEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCC
AKDMALAMIAKVGAAGAQGYAIEYAGEAVRCLSVEARMTLCNMSIEAGARCGMVAVDDKT
HHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHEEEECCHHCCCCCCEEEECCHH
IEYLRGRPMAPRGESWDLAVRTWRALVSDHDAQFDVEIEIDVGMLGPLVTWGTSPDMVIS
HHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEECCCCCCEEEE
VDEKIPDPAAEKDGTRRAGMERALRYMDLAPGTPITDVRLDRIFIGSCTNSRLEDLRAAA
ECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHH
EVIRGKHVAASIRQALVVPGSGLVKQQAENEGLHQLFIDAGFEWRDAGCSMCVGMNADHL
HHHCCHHHHHHHHHHEECCCCCHHHHHHHCCCCCEEHHHCCCCCCCCCCCEEECCCHHHH
LSGERCASTSNRNFEDRQGVGGRTHLVSPAMAAAAAIAGHFVDIRRSS
CCCCHHCCCCCCCCHHHCCCCCCCEECCHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA