BacMap

Definition	Verminephrobacter eiseniae EF01-2 chromosome, complete genome.
Accession	NC_008786
Length	5,566,749

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The map label for this gene is braF [H]

Identifier: 121611557

GI number: 121611557

Start: 5141323

End: 5142105

Strand: Direct

Name: braF [H]

Synonym: Veis_4649

Alternate gene names: 121611557

Gene position: 5141323-5142105 (Clockwise)

Preceding gene: 121611556

Following gene: 121611558

Centisome position: 92.36

GC content: 61.05

Gene sequence:

>783_bases
TTGACCGCTTCAATTCTTCTGGAAACTTTCGGCATGTCGCGGCGCTTCGGTGGGCTGCATGCCGTGAGCGATCTCGATCT
CAAGGTTCACGTCGGTGAGATCGTTGGTGTGATCGGGCCCAACGGGGCCGGCAAGTCGACCACTTTCAATATGCTCGCCG
GCGCCTTGCCACCTTCGGCGGGAGCGATTCATTTCGAGGGCCGGCGCATGGATGGTCTGCCTTCACATCGAGTGGCTGCG
CTTGGTATTGCCCGCACCTTCCAGCACAACCGGCCATTTGCCGGCATGTCGCTGACCGAGAACGTCATGGCGGGCATGCA
TCTGCATCTGCACAAGCCGCTTTGGCGTGAGATTGTCTTCTCGCACCAGGCGGCATGCGCCGAGAGAGAGGCCGCGGTCC
GGGCCGATGGACTCCTTGAATTTGTCGGGCTGGGGGATTTCAAGAAGGCGGACGTGAGCACACTGTCGTTCGGTCAGGGT
CGGCTGCTTGAAGTCGCACGCTGCATGGCGGCGAAGCCGAAATTGCTTCTGCTCGACGAGCCGGCTGCAGGCCTGACGCA
TGACGAATGCGATCGCCTCTGCGAGATTATTCGCAGCGTGGCGAAGCAGGGCGTCGCAGTACTCCTGATCGAGCACGACA
TGCGCTTGGTGATGGGGCTGACGGAACGCATCGTCGTCTTGAACTTCGGTCGAAAGATTGCCGATGGGACACCGGCCGAG
ATACGCGTCGATGCAGGCGTAGCCGCCGCATATCTTGGCGACAACGCCGAAGGGAGCGCTTGA

Upstream 100 bases:

>100_bases
ACGCCCTGATCTTTGGCATCGTCGTGGTCTTGATCATGTTGCTGGAGCCGCGTGGTTTGGTCGGCGTTTGGCTGCGTGCT
CGCTCGGCCAGGAGTTCCTC

Downstream 100 bases:

>100_bases
TGCTTGATGTTCATGATCTGTGCGGGGGCTACGGGCAGGCTCCGGTCTTGCACAACGTGAAGATGCGACTGGGCCGTGGG
GAGACCGTGGCGCTGCTCGG

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTASILLETFGMSRRFGGLHAVSDLDLKVHVGEIVGVIGPNGAGKSTTFNMLAGALPPSAGAIHFEGRRMDGLPSHRVAA
LGIARTFQHNRPFAGMSLTENVMAGMHLHLHKPLWREIVFSHQAACAEREAAVRADGLLEFVGLGDFKKADVSTLSFGQG
RLLEVARCMAAKPKLLLLDEPAAGLTHDECDRLCEIIRSVAKQGVAVLLIEHDMRLVMGLTERIVVLNFGRKIADGTPAE
IRVDAGVAAAYLGDNAEGSA

Sequences:

>Translated_260_residues
MTASILLETFGMSRRFGGLHAVSDLDLKVHVGEIVGVIGPNGAGKSTTFNMLAGALPPSAGAIHFEGRRMDGLPSHRVAA
LGIARTFQHNRPFAGMSLTENVMAGMHLHLHKPLWREIVFSHQAACAEREAAVRADGLLEFVGLGDFKKADVSTLSFGQG
RLLEVARCMAAKPKLLLLDEPAAGLTHDECDRLCEIIRSVAKQGVAVLLIEHDMRLVMGLTERIVVLNFGRKIADGTPAE
IRVDAGVAAAYLGDNAEGSA
>Mature_259_residues
TASILLETFGMSRRFGGLHAVSDLDLKVHVGEIVGVIGPNGAGKSTTFNMLAGALPPSAGAIHFEGRRMDGLPSHRVAAL
GIARTFQHNRPFAGMSLTENVMAGMHLHLHKPLWREIVFSHQAACAEREAAVRADGLLEFVGLGDFKKADVSTLSFGQGR
LLEVARCMAAKPKLLLLDEPAAGLTHDECDRLCEIIRSVAKQGVAVLLIEHDMRLVMGLTERIVVLNFGRKIADGTPAEI
RVDAGVAAAYLGDNAEGSA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=221, Percent_Identity=26.2443438914027, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI27881501, Length=230, Percent_Identity=25.2173913043478, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI30795238, Length=230, Percent_Identity=25.2173913043478, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI21536376, Length=212, Percent_Identity=28.3018867924528, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI153792144, Length=211, Percent_Identity=26.5402843601896, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI45446740, Length=218, Percent_Identity=27.9816513761468, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI47078218, Length=218, Percent_Identity=27.9816513761468, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI116734710, Length=210, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI31657092, Length=219, Percent_Identity=25.5707762557078, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI27477115, Length=212, Percent_Identity=28.3018867924528, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI150417984, Length=233, Percent_Identity=27.4678111587983, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=250, Percent_Identity=43.2, Blast_Score=210, Evalue=8e-56,
Organism=Escherichia coli, GI1789593, Length=245, Percent_Identity=34.2857142857143, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI87081791, Length=237, Percent_Identity=32.4894514767932, Blast_Score=120, Evalue=6e-29,
Organism=Escherichia coli, GI1790525, Length=234, Percent_Identity=30.3418803418803, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788210, Length=228, Percent_Identity=26.7543859649123, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789991, Length=230, Percent_Identity=29.5652173913043, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081709, Length=258, Percent_Identity=27.5193798449612, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788472, Length=243, Percent_Identity=28.3950617283951, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787370, Length=241, Percent_Identity=26.9709543568465, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786563, Length=218, Percent_Identity=31.651376146789, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788897, Length=209, Percent_Identity=29.1866028708134, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI48995001, Length=225, Percent_Identity=27.5555555555556, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87082268, Length=250, Percent_Identity=25.2, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1790190, Length=244, Percent_Identity=26.2295081967213, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787029, Length=234, Percent_Identity=27.3504273504274, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790467, Length=242, Percent_Identity=30.1652892561983, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786398, Length=226, Percent_Identity=28.3185840707965, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787792, Length=237, Percent_Identity=24.8945147679325, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786345, Length=231, Percent_Identity=31.6017316017316, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788225, Length=237, Percent_Identity=25.7383966244726, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48994943, Length=264, Percent_Identity=27.2727272727273, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787712, Length=226, Percent_Identity=26.9911504424779, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=29.4605809128631, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI87081782, Length=203, Percent_Identity=31.5270935960591, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788761, Length=242, Percent_Identity=26.0330578512397, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789672, Length=240, Percent_Identity=23.3333333333333, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994997, Length=242, Percent_Identity=26.0330578512397, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=27.4261603375527, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786319, Length=236, Percent_Identity=24.5762711864407, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=23.3050847457627, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87081834, Length=233, Percent_Identity=27.8969957081545, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790739, Length=235, Percent_Identity=27.2340425531915, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788002, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786803, Length=230, Percent_Identity=25.6521739130435, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=236, Percent_Identity=27.1186440677966, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI145693107, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082267, Length=229, Percent_Identity=28.82096069869, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789873, Length=217, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788506, Length=226, Percent_Identity=26.9911504424779, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI48994883, Length=235, Percent_Identity=27.2340425531915, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789891, Length=222, Percent_Identity=28.3783783783784, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789032, Length=245, Percent_Identity=26.9387755102041, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787759, Length=239, Percent_Identity=26.7782426778243, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787500, Length=227, Percent_Identity=24.6696035242291, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788644, Length=248, Percent_Identity=25.4032258064516, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788053, Length=209, Percent_Identity=29.1866028708134, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789586, Length=219, Percent_Identity=26.4840182648402, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787105, Length=223, Percent_Identity=24.2152466367713, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786703, Length=224, Percent_Identity=27.2321428571429, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=237, Percent_Identity=27.0042194092827, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=234, Percent_Identity=29.0598290598291, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=234, Percent_Identity=29.0598290598291, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=234, Percent_Identity=29.0598290598291, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=218, Percent_Identity=27.5229357798165, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=240, Percent_Identity=30.4166666666667, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567265, Length=212, Percent_Identity=31.6037735849057, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648314, Length=239, Percent_Identity=28.8702928870293, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573573, Length=232, Percent_Identity=30.1724137931034, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28573571, Length=232, Percent_Identity=30.1724137931034, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28571209, Length=237, Percent_Identity=24.8945147679325, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221512771, Length=232, Percent_Identity=28.0172413793103, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24666092, Length=221, Percent_Identity=27.6018099547511, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574533, Length=212, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24643674, Length=233, Percent_Identity=27.4678111587983, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28574150, Length=226, Percent_Identity=28.3185840707965, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007328, Length=226, Percent_Identity=28.3185840707965, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161076682, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI161076684, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581617, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581615, Length=200, Percent_Identity=27, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27737; Mature: 27605

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASILLETFGMSRRFGGLHAVSDLDLKVHVGEIVGVIGPNGAGKSTTFNMLAGALPPSA
CCHHHHHHHHCCHHHCCCEEEECCCEEEEEECCEEEEECCCCCCCCHHHHHHHHCCCCCC
GAIHFEGRRMDGLPSHRVAALGIARTFQHNRPFAGMSLTENVMAGMHLHLHKPLWREIVF
CEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHEECCHHHHHHHH
SHQAACAEREAAVRADGLLEFVGLGDFKKADVSTLSFGQGRLLEVARCMAAKPKLLLLDE
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCEEEEEC
PAAGLTHDECDRLCEIIRSVAKQGVAVLLIEHDMRLVMGLTERIVVLNFGRKIADGTPAE
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHEEEEEECCCHHCCCCCCE
IRVDAGVAAAYLGDNAEGSA
EEECCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TASILLETFGMSRRFGGLHAVSDLDLKVHVGEIVGVIGPNGAGKSTTFNMLAGALPPSA
CHHHHHHHHCCHHHCCCEEEECCCEEEEEECCEEEEECCCCCCCCHHHHHHHHCCCCCC
GAIHFEGRRMDGLPSHRVAALGIARTFQHNRPFAGMSLTENVMAGMHLHLHKPLWREIVF
CEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCHHHEECCHHHHHHHH
SHQAACAEREAAVRADGLLEFVGLGDFKKADVSTLSFGQGRLLEVARCMAAKPKLLLLDE
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCEEEEEC
PAAGLTHDECDRLCEIIRSVAKQGVAVLLIEHDMRLVMGLTERIVVLNFGRKIADGTPAE
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHHEEEEEECCCHHCCCCCCE
IRVDAGVAAAYLGDNAEGSA
EEECCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]