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The map label for this gene is ddl

Identifier: 121611481

GI number: 121611481

Start: 5062984

End: 5063979

Strand: Direct

Name: ddl

Synonym: Veis_4572

Alternate gene names: 121611481

Gene position: 5062984-5063979 (Clockwise)

Preceding gene: 121611480

Following gene: 121611482

Centisome position: 90.95

GC content: 70.78

Gene sequence:

>996_bases
ATGAGCCTTTTTGATCCCCATCTGGACGGAGCGTCGCTGGGCAAGGTGGCGGTGCTGATGGGCGGGGTCTCGGCCGAGCG
CGAGGTTTCGCTGCTGTCCGGCGCCGGCGTGCTGCGGGCGCTGCGCGCGCGCGCGGTCGATGCCCATGCCTTCGACACCG
CGCAGGGCGACCTGGGCGCCCTCAAACGCGAGGGCTACGCGCGCTGCTTCATCGCGCTGCATGGCCGCCATGGCGAAGAC
GGCACGGTGCAGGGCGCGCTGGAACTGCTGGGCATTGCCTACACCGGCTCCGGCGTGATGGCATCGAGCATGGCGCTGGA
CAAGACCATGAGCAAACGCATCTGGCGCAGCGAGGGCTTGCCCACGCCGGACTGGCGGCTGGTCACCAGCGGCGCCGAAG
CCGGGCAGGCCCTGCAGACGCTCGGCGCGCCGATGATCGTCAAGCCCGCGCGCGAAGGCTCGACCATCGGCCTGAGCAAG
GTGCACCAGGCGCAGCAATGCGCGTCGGCCTACCTGCTGGCCGCGCGCTACGACCCCGAGGTGCTGTGCGAGCAATTCAT
CGCCGGTGACGAGCTCACCTGCACGGTGCTCGATCAGGGCCGGCGCGCCAGCGCGCAGGCCCTGCCGCTGATCCGCATCG
TCGCGCCCGATGGCAACTACGACTACCAGCACAAGTACTTCAGCGACGCCACCCGCTACCACTGCCCGAGCGGCCTGCCC
GAGGCGCAAGAGCGCGCCATCGGGCGCCTGGCCGAGCAGGCTTTCAGCGCGCTGGGCTGCCGGGGCTGGGCGCGCGCCGA
CATCATGCTGCGCGCCAGCGACCAGCAGCCCTTTCTGCTGGAGATCAACACCGCGCCGGGCATGACGGACCACTCGCTGG
TGCCGATGTCGGCGCGCGCGGCGGGCATCAGCTACGAAGACCTGTGCCTGCGCTTGCTGGCCATGGCCACGCTGGATACG
CCGCCAGGCGCCTTGTCGGGAGCGGCCCGCGCATGA

Upstream 100 bases:

>100_bases
TGCTGCTGTGCATGGGCGCAGGCTCGATCGGCGCCGTGCCTGGCAAACTACTCGAAATGCTACGAAACGATGAGCACACC
GCAATGCAAAGGACTGCGCC

Downstream 100 bases:

>100_bases
CCATGACCGAGCCCCTGGCGCAACCGCTGGATGTCAAGTTGATGAACCTGACCGCCTCGGTCCTGTACCTGGGCTGCGCC
CTGTGCGTGCTGGCAGCGCT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSLFDPHLDGASLGKVAVLMGGVSAEREVSLLSGAGVLRALRARAVDAHAFDTAQGDLGALKREGYARCFIALHGRHGED
GTVQGALELLGIAYTGSGVMASSMALDKTMSKRIWRSEGLPTPDWRLVTSGAEAGQALQTLGAPMIVKPAREGSTIGLSK
VHQAQQCASAYLLAARYDPEVLCEQFIAGDELTCTVLDQGRRASAQALPLIRIVAPDGNYDYQHKYFSDATRYHCPSGLP
EAQERAIGRLAEQAFSALGCRGWARADIMLRASDQQPFLLEINTAPGMTDHSLVPMSARAAGISYEDLCLRLLAMATLDT
PPGALSGAARA

Sequences:

>Translated_331_residues
MSLFDPHLDGASLGKVAVLMGGVSAEREVSLLSGAGVLRALRARAVDAHAFDTAQGDLGALKREGYARCFIALHGRHGED
GTVQGALELLGIAYTGSGVMASSMALDKTMSKRIWRSEGLPTPDWRLVTSGAEAGQALQTLGAPMIVKPAREGSTIGLSK
VHQAQQCASAYLLAARYDPEVLCEQFIAGDELTCTVLDQGRRASAQALPLIRIVAPDGNYDYQHKYFSDATRYHCPSGLP
EAQERAIGRLAEQAFSALGCRGWARADIMLRASDQQPFLLEINTAPGMTDHSLVPMSARAAGISYEDLCLRLLAMATLDT
PPGALSGAARA
>Mature_330_residues
SLFDPHLDGASLGKVAVLMGGVSAEREVSLLSGAGVLRALRARAVDAHAFDTAQGDLGALKREGYARCFIALHGRHGEDG
TVQGALELLGIAYTGSGVMASSMALDKTMSKRIWRSEGLPTPDWRLVTSGAEAGQALQTLGAPMIVKPAREGSTIGLSKV
HQAQQCASAYLLAARYDPEVLCEQFIAGDELTCTVLDQGRRASAQALPLIRIVAPDGNYDYQHKYFSDATRYHCPSGLPE
AQERAIGRLAEQAFSALGCRGWARADIMLRASDQQPFLLEINTAPGMTDHSLVPMSARAAGISYEDLCLRLLAMATLDTP
PGALSGAARA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=49.1909385113269, Blast_Score=288, Evalue=2e-79,
Organism=Escherichia coli, GI1786579, Length=254, Percent_Identity=36.2204724409449, Blast_Score=148, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_VEREI (A1WRL3)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999288.1
- ProteinModelPortal:   A1WRL3
- SMR:   A1WRL3
- STRING:   A1WRL3
- GeneID:   4691354
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4572
- NMPDR:   fig|391735.5.peg.4192
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   A1WRL3
- ProtClustDB:   PRK01372
- BioCyc:   VEIS391735:VEIS_4572-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35110; Mature: 34979

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDPHLDGASLGKVAVLMGGVSAEREVSLLSGAGVLRALRARAVDAHAFDTAQGDLGA
CCCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHH
LKREGYARCFIALHGRHGEDGTVQGALELLGIAYTGSGVMASSMALDKTMSKRIWRSEGL
HHHCCCEEEEEEEECCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
PTPDWRLVTSGAEAGQALQTLGAPMIVKPAREGSTIGLSKVHQAQQCASAYLLAARYDPE
CCCCCEEEECCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
VLCEQFIAGDELTCTVLDQGRRASAQALPLIRIVAPDGNYDYQHKYFSDATRYHCPSGLP
HHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCHHCCCCCCCC
EAQERAIGRLAEQAFSALGCRGWARADIMLRASDQQPFLLEINTAPGMTDHSLVPMSARA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHH
AGISYEDLCLRLLAMATLDTPPGALSGAARA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLFDPHLDGASLGKVAVLMGGVSAEREVSLLSGAGVLRALRARAVDAHAFDTAQGDLGA
CCCCCCCCCCCCCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHH
LKREGYARCFIALHGRHGEDGTVQGALELLGIAYTGSGVMASSMALDKTMSKRIWRSEGL
HHHCCCEEEEEEEECCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
PTPDWRLVTSGAEAGQALQTLGAPMIVKPAREGSTIGLSKVHQAQQCASAYLLAARYDPE
CCCCCEEEECCCHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHH
VLCEQFIAGDELTCTVLDQGRRASAQALPLIRIVAPDGNYDYQHKYFSDATRYHCPSGLP
HHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCHHCCCCCCCC
EAQERAIGRLAEQAFSALGCRGWARADIMLRASDQQPFLLEINTAPGMTDHSLVPMSARA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCHHH
AGISYEDLCLRLLAMATLDTPPGALSGAARA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA