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The map label for this gene is yitF [H]

Identifier: 121611410

GI number: 121611410

Start: 4979327

End: 4980430

Strand: Direct

Name: yitF [H]

Synonym: Veis_4499

Alternate gene names: 121611410

Gene position: 4979327-4980430 (Clockwise)

Preceding gene: 121611407

Following gene: 121611411

Centisome position: 89.45

GC content: 59.78

Gene sequence:

>1104_bases
ATGAAAATCAATCGTCTCGAGACATTCAGCAACAGGCACGTTGGCTTTGTGCGTGTCACCACCGATACCGGTCTGCAAGG
CTGGGGGCAGATGTCCACCTATAACGCGGATATCACGGCGCAGGTGTTTCATCGGCAAATCGCGCCCTGGGCTTTGGGCG
CGAATGCCCTGGATATTGAGCATCTGGTCAATATCATCCCGGAGCGTGAGCACAAATTCCCCTGTTCCTATTTATTTCGC
GCCTTGGCCGGTCTGGACACCGCGCTGTGGGACTTGCGCGGCAAGTTGGAGCAAAAGAGCGTCTGCGAACTGTTGGGCGG
CCAGCCGCGTGCCTTTCCGGTTTATGCGTCGAGCATGAAGCGCGACATCACGCCCGAGAATGAGGCTGCGCGGCTGTCCA
GACTGCGCGACGCACATGGTTTTGGCGCCTTCAAATTCCGCGTCGGCAAGGAGTGCGGCCATGACCAGGATGAATGGCCG
GGGCGCACCGAGGCGATTGTCCCGACCATGCGCCGCGCACTCGGTGATGGCGCGCGTTTGCTGGTCGATGGCAATAGCTG
CTACACACCGGCCAAGGCGATTGCCGTCGGGCGCATGCTGGAAGAGCACGGCGTCGTCCACTTCGAGGAGCCTTGCCCCT
ATTGGGAACATCGCTGGACCAAGGAGGTCAAGGAAAGCCTGAAACTCGACGTGACCGGCGGCGAGCAGGATTGCGACCTG
ACGTTGTGGCAATACGCGATCGATCAGCGGGTGGTGGACATCGTGCAGCCCGATGTTTTTTACATCGGCGGCGTAACGCG
CATGCTGAAGGTGGCGCGCATGGCGGAGCAGGCGGGCCTGAAGGTGACGCCGCATTCGGCCAATCACTCGCTGGTGACAC
TGGTGACGCTGCACATCATGGGTGCACTCAAGAATGCCGGGGATTATGTCGAATACTCGATCGAAGGCGCAGACTATTAC
CCATGGGAACAGGGGCTGTTCCGCAACTATTTGAACGTCAAGGACGGCAAGGTGCAGATTCCCTCCGAGCCGGGCTGGGG
CCTGGAAATCTGCCCGCAATGGCTGGAAAAATCCCGTCACGCCGTCAGTGAGGTGCAGGCATGA

Upstream 100 bases:

>100_bases
TAGCATCCCCGATGGCCCAATGGCACGGCGGCTCTCGGATGGCCACGCTGATTGCAACGGGCCGTCATGAGGAGCGATAC
CCGGCCGGAGTAAATACGTA

Downstream 100 bases:

>100_bases
ACCCCTCCCTCGACGCACTGATCACCGAATTTCGCAACACCGGCACACTCAGCGGCTTGCCGTGCGCGCAATTCATCGAC
GGCGCTTATCAGGCTGGCGC

Product: mandelate racemase/muconate lactonizing protein

Products: Glyceraldehyde 3-phosphate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKINRLETFSNRHVGFVRVTTDTGLQGWGQMSTYNADITAQVFHRQIAPWALGANALDIEHLVNIIPEREHKFPCSYLFR
ALAGLDTALWDLRGKLEQKSVCELLGGQPRAFPVYASSMKRDITPENEAARLSRLRDAHGFGAFKFRVGKECGHDQDEWP
GRTEAIVPTMRRALGDGARLLVDGNSCYTPAKAIAVGRMLEEHGVVHFEEPCPYWEHRWTKEVKESLKLDVTGGEQDCDL
TLWQYAIDQRVVDIVQPDVFYIGGVTRMLKVARMAEQAGLKVTPHSANHSLVTLVTLHIMGALKNAGDYVEYSIEGADYY
PWEQGLFRNYLNVKDGKVQIPSEPGWGLEICPQWLEKSRHAVSEVQA

Sequences:

>Translated_367_residues
MKINRLETFSNRHVGFVRVTTDTGLQGWGQMSTYNADITAQVFHRQIAPWALGANALDIEHLVNIIPEREHKFPCSYLFR
ALAGLDTALWDLRGKLEQKSVCELLGGQPRAFPVYASSMKRDITPENEAARLSRLRDAHGFGAFKFRVGKECGHDQDEWP
GRTEAIVPTMRRALGDGARLLVDGNSCYTPAKAIAVGRMLEEHGVVHFEEPCPYWEHRWTKEVKESLKLDVTGGEQDCDL
TLWQYAIDQRVVDIVQPDVFYIGGVTRMLKVARMAEQAGLKVTPHSANHSLVTLVTLHIMGALKNAGDYVEYSIEGADYY
PWEQGLFRNYLNVKDGKVQIPSEPGWGLEICPQWLEKSRHAVSEVQA
>Mature_367_residues
MKINRLETFSNRHVGFVRVTTDTGLQGWGQMSTYNADITAQVFHRQIAPWALGANALDIEHLVNIIPEREHKFPCSYLFR
ALAGLDTALWDLRGKLEQKSVCELLGGQPRAFPVYASSMKRDITPENEAARLSRLRDAHGFGAFKFRVGKECGHDQDEWP
GRTEAIVPTMRRALGDGARLLVDGNSCYTPAKAIAVGRMLEEHGVVHFEEPCPYWEHRWTKEVKESLKLDVTGGEQDCDL
TLWQYAIDQRVVDIVQPDVFYIGGVTRMLKVARMAEQAGLKVTPHSANHSLVTLVTLHIMGALKNAGDYVEYSIEGADYY
PWEQGLFRNYLNVKDGKVQIPSEPGWGLEICPQWLEKSRHAVSEVQA

Specific function: D-galactonate degradation; first step. D-galactonate degradation; third step. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=315, Percent_Identity=24.1269841269841, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787864, Length=383, Percent_Identity=21.4099216710183, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI226510960, Length=283, Percent_Identity=23.321554770318, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.1.2.21; 4.2.1.6 [C]

Molecular weight: Translated: 41349; Mature: 41349

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINRLETFSNRHVGFVRVTTDTGLQGWGQMSTYNADITAQVFHRQIAPWALGANALDIE
CCCCHHHHCCCCCEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHCCCHHCCCCCCCHH
HLVNIIPEREHKFPCSYLFRALAGLDTALWDLRGKLEQKSVCELLGGQPRAFPVYASSMK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEHHHHHHH
RDITPENEAARLSRLRDAHGFGAFKFRVGKECGHDQDEWPGRTEAIVPTMRRALGDGARL
CCCCCCHHHHHHHHHHHHCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
LVDGNSCYTPAKAIAVGRMLEEHGVVHFEEPCPYWEHRWTKEVKESLKLDVTGGEQDCDL
EECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCCH
TLWQYAIDQRVVDIVQPDVFYIGGVTRMLKVARMAEQAGLKVTPHSANHSLVTLVTLHIM
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHH
GALKNAGDYVEYSIEGADYYPWEQGLFRNYLNVKDGKVQIPSEPGWGLEICPQWLEKSRH
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
AVSEVQA
HHHHHCC
>Mature Secondary Structure
MKINRLETFSNRHVGFVRVTTDTGLQGWGQMSTYNADITAQVFHRQIAPWALGANALDIE
CCCCHHHHCCCCCEEEEEEEECCCCCCCCCCEECCCCHHHHHHHHHCCCHHCCCCCCCHH
HLVNIIPEREHKFPCSYLFRALAGLDTALWDLRGKLEQKSVCELLGGQPRAFPVYASSMK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEHHHHHHH
RDITPENEAARLSRLRDAHGFGAFKFRVGKECGHDQDEWPGRTEAIVPTMRRALGDGARL
CCCCCCHHHHHHHHHHHHCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
LVDGNSCYTPAKAIAVGRMLEEHGVVHFEEPCPYWEHRWTKEVKESLKLDVTGGEQDCDL
EECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHEEEECCCCCCCCH
TLWQYAIDQRVVDIVQPDVFYIGGVTRMLKVARMAEQAGLKVTPHSANHSLVTLVTLHIM
HHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHH
GALKNAGDYVEYSIEGADYYPWEQGLFRNYLNVKDGKVQIPSEPGWGLEICPQWLEKSRH
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
AVSEVQA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate [C]

Specific reaction: 2-Dehydro-3-deoxy-D-galactonate 6-phosphate <==> Glyceraldehyde 3-phosphate + Pyruvate [C]

General reaction: elimination of H2O C-O bond; Cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]