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The map label for this gene is hisF

Identifier: 121611292

GI number: 121611292

Start: 4828553

End: 4829353

Strand: Direct

Name: hisF

Synonym: Veis_4378

Alternate gene names: 121611292

Gene position: 4828553-4829353 (Clockwise)

Preceding gene: 121611291

Following gene: 121611293

Centisome position: 86.74

GC content: 69.54

Gene sequence:

>801_bases
TTGCTGACCAAACGCATCATTGCCTGTCTGGACGTGACCGGTGGCCGTGTGGTCAAGGGCGTCAACTTCGTCGGACTGCG
CGACGCCGGGGATCCGGTCGCGGTCGCTGCGCGCTACAACGCCCAGGGGGCCGACGAGCTTGCCTTTTTGGACATCACCG
CCACCAGCGACGAGCGCGACCTGATCCTGCCCATCATCGAGGCCGTGGCCAGCCAGGTGTTCATCCCGCTGACCGTCGGC
GGCGGTGTGCGCACGCAGGCGGATGTGCGCCGCCTGCTCAACGCGGGCGCGGACAAGGTCAGCTTCAACTCGGCCGCGAT
TGCCGACCCGCAGGTGATCGATACCGCATCCGCCAAGTACGGCGCGCAGTGCATCGTGGTCGCCATCGACGCGCGGCGCC
GCTCGGCCGAGGAGTCCCGGCGCATCACCGCCGGGGGCCGGGCCATCGGCCCGGGCTGGGATGTCTACAGCCATGGCGGG
CGCAGGAACACCGGGCTCGACGCGCTGGCCTGGGCCGTCGAGATGCAGCGGCGTGGCGCCGGCGAGATCCTGCTCACCAG
CATGGACCGCGACGGCACCAGAATCGGCTTTGATCTGGAGCTGACACGCGCCGTCAGCGACGCCGTGGGCCTGCCGGTGA
TCGCCTCGGGTGGCGTGGGCACACTCGATCATCTGGCCGACGGCATACAGACCGGCGGCGCCGACGCCGTGCTGGCCGCC
AGCATCTTCCATTACGGTGAATACACGGTGGGCCAGGCCAAGCAGCATCTGGCCGGACGCGGCATTGCGGTGCGGCGCTG
A

Upstream 100 bases:

>100_bases
GAGCGGCTGCCGCAGGCTGCCCTCGCGTCGAAATCTGCCGCAGGCAGACCCCCTGCCCCGATGCCGCCTGCGGCTGCATC
GATCCAACCGGTGACCTGGC

Downstream 100 bases:

>100_bases
TGCGGCGCAATGGCCGCCCCGGGCTAGTGTCGCGTCACGGATCAGATGTCGTAGGCTGCGCGCAGCCATCCGAGCGCAGC
GCAAGGCGCAGCGCGCAGCC

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLTKRIIACLDVTGGRVVKGVNFVGLRDAGDPVAVAARYNAQGADELAFLDITATSDERDLILPIIEAVASQVFIPLTVG
GGVRTQADVRRLLNAGADKVSFNSAAIADPQVIDTASAKYGAQCIVVAIDARRRSAEESRRITAGGRAIGPGWDVYSHGG
RRNTGLDALAWAVEMQRRGAGEILLTSMDRDGTRIGFDLELTRAVSDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAA
SIFHYGEYTVGQAKQHLAGRGIAVRR

Sequences:

>Translated_266_residues
MLTKRIIACLDVTGGRVVKGVNFVGLRDAGDPVAVAARYNAQGADELAFLDITATSDERDLILPIIEAVASQVFIPLTVG
GGVRTQADVRRLLNAGADKVSFNSAAIADPQVIDTASAKYGAQCIVVAIDARRRSAEESRRITAGGRAIGPGWDVYSHGG
RRNTGLDALAWAVEMQRRGAGEILLTSMDRDGTRIGFDLELTRAVSDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAA
SIFHYGEYTVGQAKQHLAGRGIAVRR
>Mature_266_residues
MLTKRIIACLDVTGGRVVKGVNFVGLRDAGDPVAVAARYNAQGADELAFLDITATSDERDLILPIIEAVASQVFIPLTVG
GGVRTQADVRRLLNAGADKVSFNSAAIADPQVIDTASAKYGAQCIVVAIDARRRSAEESRRITAGGRAIGPGWDVYSHGG
RRNTGLDALAWAVEMQRRGAGEILLTSMDRDGTRIGFDLELTRAVSDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAA
SIFHYGEYTVGQAKQHLAGRGIAVRR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=269, Percent_Identity=42.0074349442379, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI87082028, Length=254, Percent_Identity=29.1338582677165, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=35.4632587859425, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_VEREI (A1WR24)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999099.1
- ProteinModelPortal:   A1WR24
- SMR:   A1WR24
- STRING:   A1WR24
- GeneID:   4690312
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4378
- NMPDR:   fig|391735.5.peg.4009
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A1WR24
- BioCyc:   VEIS391735:VEIS_4378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27805; Mature: 27805

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIACLDVTGGRVVKGVNFVGLRDAGDPVAVAARYNAQGADELAFLDITATSDERD
CCHHHEEEEEECCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRTQADVRRLLNAGADKVSFNSAAIADPQVIDTASAKY
HHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCEEECCCCCC
GAQCIVVAIDARRRSAEESRRITAGGRAIGPGWDVYSHGGRRNTGLDALAWAVEMQRRGA
CCEEEEEEEECCCCCHHHHCEEECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCC
GEILLTSMDRDGTRIGFDLELTRAVSDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAA
CEEEEEECCCCCCEEEEEEEHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHH
SIFHYGEYTVGQAKQHLAGRGIAVRR
HHHHCCCCCHHHHHHHHCCCCEEECC
>Mature Secondary Structure
MLTKRIIACLDVTGGRVVKGVNFVGLRDAGDPVAVAARYNAQGADELAFLDITATSDERD
CCHHHEEEEEECCCCEEEECEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRTQADVRRLLNAGADKVSFNSAAIADPQVIDTASAKY
HHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCEEECCCCCC
GAQCIVVAIDARRRSAEESRRITAGGRAIGPGWDVYSHGGRRNTGLDALAWAVEMQRRGA
CCEEEEEEEECCCCCHHHHCEEECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCC
GEILLTSMDRDGTRIGFDLELTRAVSDAVGLPVIASGGVGTLDHLADGIQTGGADAVLAA
CEEEEEECCCCCCEEEEEEEHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHH
SIFHYGEYTVGQAKQHLAGRGIAVRR
HHHHCCCCCHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA