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The map label for this gene is bcr [H]

Identifier: 121611142

GI number: 121611142

Start: 4657528

End: 4658925

Strand: Direct

Name: bcr [H]

Synonym: Veis_4226

Alternate gene names: 121611142

Gene position: 4657528-4658925 (Clockwise)

Preceding gene: 121611141

Following gene: 121611143

Centisome position: 83.67

GC content: 69.31

Gene sequence:

>1398_bases
ATGGCCAGCGCAGACCGACAGATGATCGGTGACGCGACACTCGCATTCCGGAACCCGCCATGGCCTGCCGGGGCGGAACC
CGCCGCCGATAGCGCCCTTCTTGCCTTCTGGTACGCTCGGGCCGATATGCCAGCCCCTTGCGCCAGCGCGACATCTGCGG
CCGCCCCCATCTGCCTGCATCCGGGCTTGGCCATCCTGGTGCTGGCCCTGCTGCTGAGCATACAGCCGGTCACGACCGAC
CTGTACCTGCCTGCCCTGCCCGCCATCACCAACAGCCTGGCCGCGCCGATGGCCGCCGCGCAACTCACGCTGAGCGGCCT
GTTGCTGGCGTTTGGCTGCTCGCAGATGGTCTGGGGGCCGCTGTCCGACCGCTTTGGCCGCCGCCCCATCCTGCTGACGG
GTCTGTGCATCTACACCCTGGCCTCGCTGGGCAGCGCGTTCGCATCCACCATTGCCCAACTGGTGCTGTGGCGCATTGCG
CAGGGCGCAGCAATGGGGGCCGCCGTGATGTGCGCGCGGGCCATCGTGCGCGACCTGTATACCCCGCTGGAAGGCGCGCG
CGCCATGTCCAAGGCCCTCACCGGGCTGGGCATCGTGGCCTGCGTCTGCGCGCCGCTGGGCGGGCTGCTGAGCCAATGGC
TGGGCTGGCGCGCAGCGCTGCTGGTGCTCACCGGCTATGCCCTGGTCACGCTGGCCCTGGTGGCATGGCGCTTGCCCGAG
ACCCTGGTGCAGCGCAACCCGCAGGCGTTGCAGCCGCGCACGCTATTGAGGACCTGGCAGCAGGTGTTGCGCAATCCGAC
CTTCTGGGCCTTCTCGCTGTTGACCACGACCTCCTACGGCGGCCTGTTCACCTTTCTCGCGGCGTCGCCGTTTGTCTACA
TCGATGTGCTGGGACTCACGCGCACGCAGTACGGCTGGGTCATGGCCTCGGCCTGCCTGGCTTATTTTGCCGGCACCCTG
CTGTGCCGCCGCCTGCTGAGCCGCCATGGGCTGCTGCGCACGGTGGCGATTGCCGGTGGCCTGAGCGTCAGCGGCGGCAC
GCTGCTGGCGCTGGTGGCGCTGCTGGGCTGGCACCAGCCCTGGGCGCTGCTGCTGCCGTTCTACCTGTTCATGCTCGGCC
ATGGCATGCACCAGCCCTGCGGGCAAAGCGGCGCCGTGGGGCCGTTCCCGAAGGCGGCCGGCGTGGCCTCTGCGCTCAGC
GGCTGCATGACGATGCTGGCGGCCTTTGCCATGGGCGGGTGGCTGGGCCTGCGGTTGGACGGCAGCATCTGGCCGCTGGT
CAACGGCATCTGGTTCTGGTCGGTATTGCTGGCACTGGTCGCGTGGACACTGGTGCAAAAGTTCGGTGACAGCACGGCCG
CAGGCACCTCGGCCGGAGACCGCAGTGGCCGCGCCTGA

Upstream 100 bases:

>100_bases
GTCGTAGGCTGCGCGCAGCCATCGGAGCGCAGCGCAAGGCGCAGCGCGCAGCCCATACCGGGCTGTATTGGCAAGCGATG
CAACGCCGCGCTACGCTTCG

Downstream 100 bases:

>100_bases
CAGCCGGCTCCCGAACATCGCCCTGGCAGGCCCCACGGCGGCGGGCAAGACGGCGGCGGCGCTGGCGCTGGCGGCGGCGC
TGGGCCGGCGCCAAGCGGTG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MASADRQMIGDATLAFRNPPWPAGAEPAADSALLAFWYARADMPAPCASATSAAAPICLHPGLAILVLALLLSIQPVTTD
LYLPALPAITNSLAAPMAAAQLTLSGLLLAFGCSQMVWGPLSDRFGRRPILLTGLCIYTLASLGSAFASTIAQLVLWRIA
QGAAMGAAVMCARAIVRDLYTPLEGARAMSKALTGLGIVACVCAPLGGLLSQWLGWRAALLVLTGYALVTLALVAWRLPE
TLVQRNPQALQPRTLLRTWQQVLRNPTFWAFSLLTTTSYGGLFTFLAASPFVYIDVLGLTRTQYGWVMASACLAYFAGTL
LCRRLLSRHGLLRTVAIAGGLSVSGGTLLALVALLGWHQPWALLLPFYLFMLGHGMHQPCGQSGAVGPFPKAAGVASALS
GCMTMLAAFAMGGWLGLRLDGSIWPLVNGIWFWSVLLALVAWTLVQKFGDSTAAGTSAGDRSGRA

Sequences:

>Translated_465_residues
MASADRQMIGDATLAFRNPPWPAGAEPAADSALLAFWYARADMPAPCASATSAAAPICLHPGLAILVLALLLSIQPVTTD
LYLPALPAITNSLAAPMAAAQLTLSGLLLAFGCSQMVWGPLSDRFGRRPILLTGLCIYTLASLGSAFASTIAQLVLWRIA
QGAAMGAAVMCARAIVRDLYTPLEGARAMSKALTGLGIVACVCAPLGGLLSQWLGWRAALLVLTGYALVTLALVAWRLPE
TLVQRNPQALQPRTLLRTWQQVLRNPTFWAFSLLTTTSYGGLFTFLAASPFVYIDVLGLTRTQYGWVMASACLAYFAGTL
LCRRLLSRHGLLRTVAIAGGLSVSGGTLLALVALLGWHQPWALLLPFYLFMLGHGMHQPCGQSGAVGPFPKAAGVASALS
GCMTMLAAFAMGGWLGLRLDGSIWPLVNGIWFWSVLLALVAWTLVQKFGDSTAAGTSAGDRSGRA
>Mature_464_residues
ASADRQMIGDATLAFRNPPWPAGAEPAADSALLAFWYARADMPAPCASATSAAAPICLHPGLAILVLALLLSIQPVTTDL
YLPALPAITNSLAAPMAAAQLTLSGLLLAFGCSQMVWGPLSDRFGRRPILLTGLCIYTLASLGSAFASTIAQLVLWRIAQ
GAAMGAAVMCARAIVRDLYTPLEGARAMSKALTGLGIVACVCAPLGGLLSQWLGWRAALLVLTGYALVTLALVAWRLPET
LVQRNPQALQPRTLLRTWQQVLRNPTFWAFSLLTTTSYGGLFTFLAASPFVYIDVLGLTRTQYGWVMASACLAYFAGTLL
CRRLLSRHGLLRTVAIAGGLSVSGGTLLALVALLGWHQPWALLLPFYLFMLGHGMHQPCGQSGAVGPFPKAAGVASALSG
CMTMLAAFAMGGWLGLRLDGSIWPLVNGIWFWSVLLALVAWTLVQKFGDSTAAGTSAGDRSGRA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=374, Percent_Identity=29.9465240641711, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI48994889, Length=354, Percent_Identity=29.0960451977401, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082312, Length=325, Percent_Identity=34.1538461538462, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1790794, Length=251, Percent_Identity=27.4900398406374, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790146, Length=240, Percent_Identity=27.9166666666667, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787065, Length=279, Percent_Identity=26.8817204301075, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=310, Percent_Identity=21.2903225806452, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49001; Mature: 48870

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00092 N6_MTASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASADRQMIGDATLAFRNPPWPAGAEPAADSALLAFWYARADMPAPCASATSAAAPICLH
CCCCCHHHHCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHC
PGLAILVLALLLSIQPVTTDLYLPALPAITNSLAAPMAAAQLTLSGLLLAFGCSQMVWGP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSDRFGRRPILLTGLCIYTLASLGSAFASTIAQLVLWRIAQGAAMGAAVMCARAIVRDLY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPLEGARAMSKALTGLGIVACVCAPLGGLLSQWLGWRAALLVLTGYALVTLALVAWRLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TLVQRNPQALQPRTLLRTWQQVLRNPTFWAFSLLTTTSYGGLFTFLAASPFVYIDVLGLT
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEEHHHH
RTQYGWVMASACLAYFAGTLLCRRLLSRHGLLRTVAIAGGLSVSGGTLLALVALLGWHQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
WALLLPFYLFMLGHGMHQPCGQSGAVGPFPKAAGVASALSGCMTMLAAFAMGGWLGLRLD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GSIWPLVNGIWFWSVLLALVAWTLVQKFGDSTAAGTSAGDRSGRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASADRQMIGDATLAFRNPPWPAGAEPAADSALLAFWYARADMPAPCASATSAAAPICLH
CCCCHHHHCCHHEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHC
PGLAILVLALLLSIQPVTTDLYLPALPAITNSLAAPMAAAQLTLSGLLLAFGCSQMVWGP
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSDRFGRRPILLTGLCIYTLASLGSAFASTIAQLVLWRIAQGAAMGAAVMCARAIVRDLY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TPLEGARAMSKALTGLGIVACVCAPLGGLLSQWLGWRAALLVLTGYALVTLALVAWRLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
TLVQRNPQALQPRTLLRTWQQVLRNPTFWAFSLLTTTSYGGLFTFLAASPFVYIDVLGLT
HHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCEEEEEEHHHH
RTQYGWVMASACLAYFAGTLLCRRLLSRHGLLRTVAIAGGLSVSGGTLLALVALLGWHQP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
WALLLPFYLFMLGHGMHQPCGQSGAVGPFPKAAGVASALSGCMTMLAAFAMGGWLGLRLD
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GSIWPLVNGIWFWSVLLALVAWTLVQKFGDSTAAGTSAGDRSGRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]