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The map label for this gene is kshB [H]

Identifier: 121610974

GI number: 121610974

Start: 4451040

End: 4452191

Strand: Direct

Name: kshB [H]

Synonym: Veis_4058

Alternate gene names: 121610974

Gene position: 4451040-4452191 (Clockwise)

Preceding gene: 121610973

Following gene: 121610975

Centisome position: 79.96

GC content: 67.88

Gene sequence:

>1152_bases
ATGCCCACCGTTGCTTTGCCTGGTTCACCGGGTTCACCGGACCTTCCCGATTTCGCGGCCCCGGCCCCGCCGCCCTGGTC
CGCCGAGCAAGACCACAGCCTGCGCTGCGTGCATATCCGGCAAGAGACCCATGACGTCAAAACCTTCGTGCTGGCACCGC
GCGCGCCGCGCAGCTTTCGCTACCTGCCGGGCCAATTCATCACGCTGGAGCTGGACATCGCGGGCCGCAGGATCAACCGC
TGCTACACCCTGTCATCGACCCCGACCCGCCCCGACTTGGTCAGCATCACGGTCAAACGGGTGCCGGGCGGCCCGGTGTC
GAACTGGCTGCACGAGCAGTTGCGCGTGGGCATGGCGCTGGACGTGCTGGGCCCCGGCGGCGCGTTCAGTTGTTTGGCAG
CGCCAGCGCAGCGCTACCTGTTTCTATCGGGCGGCAGCGGCATCACGCCGCTGATGTCGATGACGCGGGCGCTGCACGAC
CTGGGCAGCGACGCCGACATCGTGTTCGTGCACTGCGCGCGCAGCCCCGCCGATGTGCTGTTTTCCGACGAGTTGGGCCT
GATGGCGCGCCACATGCCGCATCTGCGGCTGGCCACGGTGTGCGAGCAACAAGCGCCCGGCAGCGCCTATGCCGGCCATC
TGGGGCGCCTGGATGCGGCGCTGCTCGCGCGCATCGCCCCGGACTTGCAGCAGCGCGACATCTATACCTGCGGGCCGGCG
CCGTTCATGGCCGCGATCCATGCGTATCTGTCCGGCGCGGGCTTTCCGATGGCCCGCTACCGGCAAGAGAGCTTCGCGTT
CGAATCGTTGGCGCAGCCCGTGGCCACACCGATGCCAGCCGCCGGCGCCAGCACCGCGCCAGCCCACGCCAGCCCCCGAA
CCGGCGCCCCCGCCTACCAGGTGCGCTTGCAAAAAACCGGCCACCAATTCGATTGCCCAGCCGAGCAAACCCTGCTGCAG
GCGGCCATCGCGGCCGGACTGCGGCTGCCTTTTTCCTGCACCAGCGGCGCCTGCGGCACCTGCAAAAGCAAAAAAATCGC
GGGCCAGGTCCGCATCGAACATGCCGGCGGCATCCGGCAACGAGAAATCGATCAGGGCTGGATCCTGCCCTGCTGCAGCA
AGCCTTTGTCTGACATCGTGCTGGACCGATAG

Upstream 100 bases:

>100_bases
GAAGGCTTGGTCGAGAAGTTTTGCAACTGGTATGTGAACCGCCTGCGCGCCCTGGTCCGCTGAACGCCCGCCCAACACCC
GCTCCTGCCGCGAGCCACCC

Downstream 100 bases:

>100_bases
CGCAGCGATGGCATCTCGTTTACAGTGTTTACGGTTCATCGGGAGGTAAGAACCATTGTCGATGGCTCGATGGCCATCCC
GCAAATGGCCATGGCGCACA

Product: oxidoreductase FAD-binding subunit

Products: NA

Alternate protein names: Rieske-type oxygenase; RO [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPTVALPGSPGSPDLPDFAAPAPPPWSAEQDHSLRCVHIRQETHDVKTFVLAPRAPRSFRYLPGQFITLELDIAGRRINR
CYTLSSTPTRPDLVSITVKRVPGGPVSNWLHEQLRVGMALDVLGPGGAFSCLAAPAQRYLFLSGGSGITPLMSMTRALHD
LGSDADIVFVHCARSPADVLFSDELGLMARHMPHLRLATVCEQQAPGSAYAGHLGRLDAALLARIAPDLQQRDIYTCGPA
PFMAAIHAYLSGAGFPMARYRQESFAFESLAQPVATPMPAAGASTAPAHASPRTGAPAYQVRLQKTGHQFDCPAEQTLLQ
AAIAAGLRLPFSCTSGACGTCKSKKIAGQVRIEHAGGIRQREIDQGWILPCCSKPLSDIVLDR

Sequences:

>Translated_383_residues
MPTVALPGSPGSPDLPDFAAPAPPPWSAEQDHSLRCVHIRQETHDVKTFVLAPRAPRSFRYLPGQFITLELDIAGRRINR
CYTLSSTPTRPDLVSITVKRVPGGPVSNWLHEQLRVGMALDVLGPGGAFSCLAAPAQRYLFLSGGSGITPLMSMTRALHD
LGSDADIVFVHCARSPADVLFSDELGLMARHMPHLRLATVCEQQAPGSAYAGHLGRLDAALLARIAPDLQQRDIYTCGPA
PFMAAIHAYLSGAGFPMARYRQESFAFESLAQPVATPMPAAGASTAPAHASPRTGAPAYQVRLQKTGHQFDCPAEQTLLQ
AAIAAGLRLPFSCTSGACGTCKSKKIAGQVRIEHAGGIRQREIDQGWILPCCSKPLSDIVLDR
>Mature_382_residues
PTVALPGSPGSPDLPDFAAPAPPPWSAEQDHSLRCVHIRQETHDVKTFVLAPRAPRSFRYLPGQFITLELDIAGRRINRC
YTLSSTPTRPDLVSITVKRVPGGPVSNWLHEQLRVGMALDVLGPGGAFSCLAAPAQRYLFLSGGSGITPLMSMTRALHDL
GSDADIVFVHCARSPADVLFSDELGLMARHMPHLRLATVCEQQAPGSAYAGHLGRLDAALLARIAPDLQQRDIYTCGPAP
FMAAIHAYLSGAGFPMARYRQESFAFESLAQPVATPMPAAGASTAPAHASPRTGAPAYQVRLQKTGHQFDCPAEQTLLQA
AIAAGLRLPFSCTSGACGTCKSKKIAGQVRIEHAGGIRQREIDQGWILPCCSKPLSDIVLDR

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta-cleavage [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787098, Length=344, Percent_Identity=30.8139534883721, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787658, Length=361, Percent_Identity=26.8698060941828, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1788104, Length=351, Percent_Identity=26.2108262108262, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788903, Length=213, Percent_Identity=29.1079812206573, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40968; Mature: 40836

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVALPGSPGSPDLPDFAAPAPPPWSAEQDHSLRCVHIRQETHDVKTFVLAPRAPRSFR
CCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCE
YLPGQFITLELDIAGRRINRCYTLSSTPTRPDLVSITVKRVPGGPVSNWLHEQLRVGMAL
ECCCEEEEEEEEECCCEECEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCEEE
DVLGPGGAFSCLAAPAQRYLFLSGGSGITPLMSMTRALHDLGSDADIVFVHCARSPADVL
EECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEE
FSDELGLMARHMPHLRLATVCEQQAPGSAYAGHLGRLDAALLARIAPDLQQRDIYTCGPA
ECCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEECCCH
PFMAAIHAYLSGAGFPMARYRQESFAFESLAQPVATPMPAAGASTAPAHASPRTGAPAYQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
VRLQKTGHQFDCPAEQTLLQAAIAAGLRLPFSCTSGACGTCKSKKIAGQVRIEHAGGIRQ
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCH
REIDQGWILPCCSKPLSDIVLDR
HCCCCCCCCHHCCCCHHHHHCCC
>Mature Secondary Structure 
PTVALPGSPGSPDLPDFAAPAPPPWSAEQDHSLRCVHIRQETHDVKTFVLAPRAPRSFR
CCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCE
YLPGQFITLELDIAGRRINRCYTLSSTPTRPDLVSITVKRVPGGPVSNWLHEQLRVGMAL
ECCCEEEEEEEEECCCEECEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHCEEE
DVLGPGGAFSCLAAPAQRYLFLSGGSGITPLMSMTRALHDLGSDADIVFVHCARSPADVL
EECCCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEE
FSDELGLMARHMPHLRLATVCEQQAPGSAYAGHLGRLDAALLARIAPDLQQRDIYTCGPA
ECCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEECCCH
PFMAAIHAYLSGAGFPMARYRQESFAFESLAQPVATPMPAAGASTAPAHASPRTGAPAYQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
VRLQKTGHQFDCPAEQTLLQAAIAAGLRLPFSCTSGACGTCKSKKIAGQVRIEHAGGIRQ
EEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCH
REIDQGWILPCCSKPLSDIVLDR
HCCCCCCCCHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA