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The map label for this gene is fabI [H]

Identifier: 121610735

GI number: 121610735

Start: 4210855

End: 4211655

Strand: Direct

Name: fabI [H]

Synonym: Veis_3815

Alternate gene names: 121610735

Gene position: 4210855-4211655 (Clockwise)

Preceding gene: 121610729

Following gene: 121610737

Centisome position: 75.64

GC content: 68.04

Gene sequence:

>801_bases
ATGGGTTTTTTGACCGGCAAAAAGCTGCTCATCACGGGCCTGCTATCGAACCGCTCCATCGCCTACGGCATCGCCCGGGC
CTGCCGCGCGCAGGGCGCCGAGCTGGCTTTCAGCTATGTCGGCGAGCGCTTCAAGGCGCGCACCGGCGACTTTGCCGCCG
AGTTCGATTCCAGGCTGGTCTTTGACTGCGATGTCGCCAGCGATGAGCAGATCGGGCGCCTGTTTGCCGACCTGGCCCAG
ACCTGGCCGCGCTTCGATGGCTTGGTGCACAGCATCGGCTTTGCGCCGCGCGAGGCGCTGGCCGGCAACTTTCTCGCCGG
CCTGTCGCGCGAGGGCTTTCGCAGCGCGCACGACATCAGCGCCTACAGCTTCCCGGCCATGGCCAAGGCCGCCCTGCCCT
ACCTGAACGACCGGTCCGCGCTGCTGACGCTGTCGTACCTGGGCGCGCTGCGCGCCATCCCGAACTACAACACCATGGGC
CTGGCCAAGGCCAGTCTGGAAGCCTCGGTGCGCTACCTGGCCGAGGCCGTGGGCCGCACCGAAGACGGCCGCAGCATCCG
TGCCAACGGCATCAGCGCCGGCCCGATCAAGACCCTGGCCGCCAGCGGCATCCAGGACTTCGGCAAGCTGCTCGGCCGCG
TGGCCGGCGCCAGCCCGTTGCGGCGCAACGTGACCCTCGACGAGGTGGGCAATGTGGCCGCCTTTTTGCTGTCCGACCTG
GCCTCGGGCGTGACCGCAGAGATCGTCTATGTGGACGCCGGCTTTGGCCAGACCGCCGGCCTGAGTGTCGATCAGGTCTG
A

Upstream 100 bases:

>100_bases
GTATCCCCGCGCGCCAGGCAACCGTGGCCAACGACCGGCGCGCAGGGGCCCCTGCCGGGGCCGGCAACGCCAATGTCCCG
CATTCCAAAGGAGAACACCC

Downstream 100 bases:

>100_bases
GCCGCCTCATTCCATTGATGGAATCACTTGTTTTTCCAGCCCCTGCCCGCCACCGGGTTGGCGGCCCCGATCGGGCGCGG
CGGCAGGCCGGTGTGCTGCG

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGFLTGKKLLITGLLSNRSIAYGIARACRAQGAELAFSYVGERFKARTGDFAAEFDSRLVFDCDVASDEQIGRLFADLAQ
TWPRFDGLVHSIGFAPREALAGNFLAGLSREGFRSAHDISAYSFPAMAKAALPYLNDRSALLTLSYLGALRAIPNYNTMG
LAKASLEASVRYLAEAVGRTEDGRSIRANGISAGPIKTLAASGIQDFGKLLGRVAGASPLRRNVTLDEVGNVAAFLLSDL
ASGVTAEIVYVDAGFGQTAGLSVDQV

Sequences:

>Translated_266_residues
MGFLTGKKLLITGLLSNRSIAYGIARACRAQGAELAFSYVGERFKARTGDFAAEFDSRLVFDCDVASDEQIGRLFADLAQ
TWPRFDGLVHSIGFAPREALAGNFLAGLSREGFRSAHDISAYSFPAMAKAALPYLNDRSALLTLSYLGALRAIPNYNTMG
LAKASLEASVRYLAEAVGRTEDGRSIRANGISAGPIKTLAASGIQDFGKLLGRVAGASPLRRNVTLDEVGNVAAFLLSDL
ASGVTAEIVYVDAGFGQTAGLSVDQV
>Mature_265_residues
GFLTGKKLLITGLLSNRSIAYGIARACRAQGAELAFSYVGERFKARTGDFAAEFDSRLVFDCDVASDEQIGRLFADLAQT
WPRFDGLVHSIGFAPREALAGNFLAGLSREGFRSAHDISAYSFPAMAKAALPYLNDRSALLTLSYLGALRAIPNYNTMGL
AKASLEASVRYLAEAVGRTEDGRSIRANGISAGPIKTLAASGIQDFGKLLGRVAGASPLRRNVTLDEVGNVAAFLLSDLA
SGVTAEIVYVDAGFGQTAGLSVDQV

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=255, Percent_Identity=58.8235294117647, Blast_Score=310, Evalue=8e-86,
Organism=Escherichia coli, GI87082100, Length=272, Percent_Identity=27.9411764705882, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28110; Mature: 27979

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLTGKKLLITGLLSNRSIAYGIARACRAQGAELAFSYVGERFKARTGDFAAEFDSRLV
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCEEE
FDCDVASDEQIGRLFADLAQTWPRFDGLVHSIGFAPREALAGNFLAGLSREGFRSAHDIS
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCCHHHCC
AYSFPAMAKAALPYLNDRSALLTLSYLGALRAIPNYNTMGLAKASLEASVRYLAEAVGRT
HHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EDGRSIRANGISAGPIKTLAASGIQDFGKLLGRVAGASPLRRNVTLDEVGNVAAFLLSDL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHH
ASGVTAEIVYVDAGFGQTAGLSVDQV
HCCCEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
GFLTGKKLLITGLLSNRSIAYGIARACRAQGAELAFSYVGERFKARTGDFAAEFDSRLV
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCEEE
FDCDVASDEQIGRLFADLAQTWPRFDGLVHSIGFAPREALAGNFLAGLSREGFRSAHDIS
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHCCCHHHCC
AYSFPAMAKAALPYLNDRSALLTLSYLGALRAIPNYNTMGLAKASLEASVRYLAEAVGRT
HHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EDGRSIRANGISAGPIKTLAASGIQDFGKLLGRVAGASPLRRNVTLDEVGNVAAFLLSDL
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHH
ASGVTAEIVYVDAGFGQTAGLSVDQV
HCCCEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]