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The map label for this gene is nth [H]

Identifier: 121609421

GI number: 121609421

Start: 2800603

End: 2801241

Strand: Direct

Name: nth [H]

Synonym: Veis_2465

Alternate gene names: 121609421

Gene position: 2800603-2801241 (Clockwise)

Preceding gene: 121609418

Following gene: 121609430

Centisome position: 50.31

GC content: 66.98

Gene sequence:

>639_bases
ATGCAACGAGAATCCATAGCCCCCTTCTTCGCCGCGCTGCAAGCGGCCAACCCCACGCCCGGCACCGAGCTGGAATACAC
CAGCGTGTTCGAGCTATTGACCGCCGTGCTGCTATCGGCCCAGGCCACCGATGTCGGCGTGAACAAGGCCACGCGTCGGC
TGTTTGCGGTGGCGAACACGCCGCAGGCGATGCTGGACCTGGGCTTGGCCGGCCTGGAAAGCCATATCCGCACCATTGGC
CTGTACAAAAGCAAGGCGCGCCACCTGCTGCACAGTTGCCGCATCCTTGTCGAGCACCATGGCGGCGTGGTGCCGCGCAC
GCGCGAGGCTTTGCAGACCCTGCCCGGCGTGGGCCGCAAGACGGCCAATGTGGTGCTGAACGTGGCCTTCGGCGAGCCGA
CGATGGCGGTGGACAGGCATATCTTTCGCGTCAGCAACCGCACCGGCCTGGCCCCGGGCAAGAACCCGCTGGCGGTGGAG
CTGCAACTCCTGCAGCGCGTGCCGCAGACCTGCGCGGTCGACGCGCACCACTGGCTGATCCTGCTGGGCCGCTATGTGTG
CCAGGCCCGCAAACCCCGCTGCCAGCAATGCCTGGTGGCCGCCTACTGCGACTTCAGGGCCAACACGCTGGCGCAGTGA

Upstream 100 bases:

>100_bases
GCCGATCGACGGGGCCGGTGCAGCGGCTGGCCAGACCCGCCCTCAGCCACTACCATCGGCATGGCCCTGCGCCCCCAATA
CCCCATGCCCCCATGCCCCC

Downstream 100 bases:

>100_bases
CGGGCGGTGTGACGGCGCATCGTCACCGGGCCGGCATCGACGGGGCAGCGTCTCGGCACTTTGCCGGCCGGCCGGCTATT
GCGGCCTGGGGGCCAGATAC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQRESIAPFFAALQAANPTPGTELEYTSVFELLTAVLLSAQATDVGVNKATRRLFAVANTPQAMLDLGLAGLESHIRTIG
LYKSKARHLLHSCRILVEHHGGVVPRTREALQTLPGVGRKTANVVLNVAFGEPTMAVDRHIFRVSNRTGLAPGKNPLAVE
LQLLQRVPQTCAVDAHHWLILLGRYVCQARKPRCQQCLVAAYCDFRANTLAQ

Sequences:

>Translated_212_residues
MQRESIAPFFAALQAANPTPGTELEYTSVFELLTAVLLSAQATDVGVNKATRRLFAVANTPQAMLDLGLAGLESHIRTIG
LYKSKARHLLHSCRILVEHHGGVVPRTREALQTLPGVGRKTANVVLNVAFGEPTMAVDRHIFRVSNRTGLAPGKNPLAVE
LQLLQRVPQTCAVDAHHWLILLGRYVCQARKPRCQQCLVAAYCDFRANTLAQ
>Mature_212_residues
MQRESIAPFFAALQAANPTPGTELEYTSVFELLTAVLLSAQATDVGVNKATRRLFAVANTPQAMLDLGLAGLESHIRTIG
LYKSKARHLLHSCRILVEHHGGVVPRTREALQTLPGVGRKTANVVLNVAFGEPTMAVDRHIFRVSNRTGLAPGKNPLAVE
LQLLQRVPQTCAVDAHHWLILLGRYVCQARKPRCQQCLVAAYCDFRANTLAQ

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=193, Percent_Identity=31.0880829015544, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI115298650, Length=151, Percent_Identity=37.7483443708609, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI115298654, Length=151, Percent_Identity=37.7483443708609, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI115298652, Length=151, Percent_Identity=37.7483443708609, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI190358497, Length=151, Percent_Identity=37.7483443708609, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI6912520, Length=151, Percent_Identity=37.7483443708609, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI115298648, Length=151, Percent_Identity=37.7483443708609, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1787920, Length=194, Percent_Identity=62.8865979381443, Blast_Score=256, Evalue=6e-70,
Organism=Escherichia coli, GI1789331, Length=151, Percent_Identity=31.1258278145695, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=33.1428571428571, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.6666666666667, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23195; Mature: 23195

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRESIAPFFAALQAANPTPGTELEYTSVFELLTAVLLSAQATDVGVNKATRRLFAVANT
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
PQAMLDLGLAGLESHIRTIGLYKSKARHLLHSCRILVEHHGGVVPRTREALQTLPGVGRK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
TANVVLNVAFGEPTMAVDRHIFRVSNRTGLAPGKNPLAVELQLLQRVPQTCAVDAHHWLI
CEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHH
LLGRYVCQARKPRCQQCLVAAYCDFRANTLAQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRESIAPFFAALQAANPTPGTELEYTSVFELLTAVLLSAQATDVGVNKATRRLFAVANT
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
PQAMLDLGLAGLESHIRTIGLYKSKARHLLHSCRILVEHHGGVVPRTREALQTLPGVGRK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCC
TANVVLNVAFGEPTMAVDRHIFRVSNRTGLAPGKNPLAVELQLLQRVPQTCAVDAHHWLI
CEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCHHHHHH
LLGRYVCQARKPRCQQCLVAAYCDFRANTLAQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]