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The map label for this gene is gltD [H]

Identifier: 121609353

GI number: 121609353

Start: 2713987

End: 2715465

Strand: Direct

Name: gltD [H]

Synonym: Veis_2396

Alternate gene names: 121609353

Gene position: 2713987-2715465 (Clockwise)

Preceding gene: 121609352

Following gene: 121609354

Centisome position: 48.75

GC content: 67.61

Gene sequence:

>1479_bases
ATGGGAAAGACCACCGGCTTCATGGAACACGGGCGCATCGAAGAAGGCCACCAGAGCGTTGCCGAGCGACTCAGGCATTA
CAAGGAATTCGTCATCGGCCTCGACGAGGACCAGGCCCGGGTGCAGGGCGCGCGCTGCATGGACTGCGGCACGCCGTTTT
GCAGCCATGGCTGCCCGGTGAACAACATCATTCCCGACTTCAACGACCTGATCTACCAGGCCGACTGGAAGGGCGCGCTC
GAAGTACTGCACAGCACCAACAACTTCCCCGAGTTCACCGGCCGCATTTGCCCCGCGCCCTGCGAGGCCGCCTGCGTGCT
GAACGTGAACGACAAGGCCGTGGGCATCAAGTCGATCGAGCACGCGATCATCGACCGCGCCTGGCAACAGGGCTGGGTGC
AGCCCCGCCCGGCCCGGCACCCCAGCGGCAAGAAGGTGGCCGTGGTCGGCTCCGGCCCGGCCGGCATGGCCGCAGCGCAG
CAACTGGCGCGCGCCGGCCACGGCGTCACGCTGTTCGAGAAGAATGACCGCGTGGGCGGTCTGCTGCGCTACGGCATCCC
GGACTTCAAGATGGAAAAATCGCACATCGACCGGCGCATCGCGCAGCTACAGGCCGAAGGCGTGGTCTTGCGCCCGGGGG
TGTTCGTGGGCTCGGCCAGCGAGGGCCTGGGCAAAGGCTCCAAGGTCGGCAACGCAGCCAAGGAAACCGTGACGCCCGAG
CAGTTGTACGCGCAGTTCGATGCCGTGCTGCTGACCGGCGGCGCCGAGCAGTCACGCGACCTGCCGGTGCCCGGCCGCGG
GTTGGACGGCATCCACTTCGCGATGGAATTCCTGCCCCAGCAAAACAAGGTCAATGCCGGCGACAAGCTCAAGGGGCAGT
TGCGCGCGGACGGCAAGCATGTGATCGTGATCGGCGGCGGCGACACCGGCAGCGACTGCGTGGGCACCAGCCGCCGCCAT
GGCGCTGCCAGCGTCACGCAGTTCGAGCTGCTGCCCCGGCCGCCAGAAGAAGAAAACCGCCCGCTGACCTGGCCCTACTG
GCCGATCAAGCTGCGCACCAGTTCCAGCCACGAAGAGGGCTGCGCGCGCGAGTTCGCCATCGCCACCCGGGAGTTCGTCG
GCGACAAAGGCCGTGTGACGGGCCTGAAAACCGTGCGCGTGCAGTTCAAGGACGGCAAACTGGTCGAAATCGCCGGCTCG
GAACAGACCCTCCCGGCCGACCTGGTGCTGCTGGCCATGGGTTTCGTCGGCCCGGTGGCGCCGGTGCTCGACGCCTTTGG
GGTGGAGCAGGATGCCCGTGGCAATGCCCGCGCCAGCACCGAACCCGACGGCGGCTACGCCACCAACGTGCCCAAGGTCT
TTGCCGCCGGCGACATGCGCCGGGGCCAGTCGCTGGTCGTCTGGGCCATCCGCGAGGGCCGGCAGGCGGCCCATGCGGTC
GATCGGTTTCTGATGGGGCATTCCGAATTGCCCCGCTGA

Upstream 100 bases:

>100_bases
TGAGGGCGCGCAACGAAGGCGTGCAGTCAGGGCGCGCGGCACAGGCGCGCGCGCAACCCATCGATCCATCAGTACATCGG
CACCAATGCAAGGACATCCC

Downstream 100 bases:

>100_bases
CCGGGATGGCGGCGGGATTTGTGGGTACCATCGACGGTTTCCTGCCGGCCGACCCCAGGCGGGGCTATCTGGCCGTGGCG
GCTCGCCGTCGGCATCAGGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MGKTTGFMEHGRIEEGHQSVAERLRHYKEFVIGLDEDQARVQGARCMDCGTPFCSHGCPVNNIIPDFNDLIYQADWKGAL
EVLHSTNNFPEFTGRICPAPCEAACVLNVNDKAVGIKSIEHAIIDRAWQQGWVQPRPARHPSGKKVAVVGSGPAGMAAAQ
QLARAGHGVTLFEKNDRVGGLLRYGIPDFKMEKSHIDRRIAQLQAEGVVLRPGVFVGSASEGLGKGSKVGNAAKETVTPE
QLYAQFDAVLLTGGAEQSRDLPVPGRGLDGIHFAMEFLPQQNKVNAGDKLKGQLRADGKHVIVIGGGDTGSDCVGTSRRH
GAASVTQFELLPRPPEEENRPLTWPYWPIKLRTSSSHEEGCAREFAIATREFVGDKGRVTGLKTVRVQFKDGKLVEIAGS
EQTLPADLVLLAMGFVGPVAPVLDAFGVEQDARGNARASTEPDGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAAHAV
DRFLMGHSELPR

Sequences:

>Translated_492_residues
MGKTTGFMEHGRIEEGHQSVAERLRHYKEFVIGLDEDQARVQGARCMDCGTPFCSHGCPVNNIIPDFNDLIYQADWKGAL
EVLHSTNNFPEFTGRICPAPCEAACVLNVNDKAVGIKSIEHAIIDRAWQQGWVQPRPARHPSGKKVAVVGSGPAGMAAAQ
QLARAGHGVTLFEKNDRVGGLLRYGIPDFKMEKSHIDRRIAQLQAEGVVLRPGVFVGSASEGLGKGSKVGNAAKETVTPE
QLYAQFDAVLLTGGAEQSRDLPVPGRGLDGIHFAMEFLPQQNKVNAGDKLKGQLRADGKHVIVIGGGDTGSDCVGTSRRH
GAASVTQFELLPRPPEEENRPLTWPYWPIKLRTSSSHEEGCAREFAIATREFVGDKGRVTGLKTVRVQFKDGKLVEIAGS
EQTLPADLVLLAMGFVGPVAPVLDAFGVEQDARGNARASTEPDGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAAHAV
DRFLMGHSELPR
>Mature_491_residues
GKTTGFMEHGRIEEGHQSVAERLRHYKEFVIGLDEDQARVQGARCMDCGTPFCSHGCPVNNIIPDFNDLIYQADWKGALE
VLHSTNNFPEFTGRICPAPCEAACVLNVNDKAVGIKSIEHAIIDRAWQQGWVQPRPARHPSGKKVAVVGSGPAGMAAAQQ
LARAGHGVTLFEKNDRVGGLLRYGIPDFKMEKSHIDRRIAQLQAEGVVLRPGVFVGSASEGLGKGSKVGNAAKETVTPEQ
LYAQFDAVLLTGGAEQSRDLPVPGRGLDGIHFAMEFLPQQNKVNAGDKLKGQLRADGKHVIVIGGGDTGSDCVGTSRRHG
AASVTQFELLPRPPEEENRPLTWPYWPIKLRTSSSHEEGCAREFAIATREFVGDKGRVTGLKTVRVQFKDGKLVEIAGSE
QTLPADLVLLAMGFVGPVAPVLDAFGVEQDARGNARASTEPDGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAAHAVD
RFLMGHSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=478, Percent_Identity=25.7322175732218, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789606, Length=490, Percent_Identity=34.8979591836735, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1788811, Length=487, Percent_Identity=33.4702258726899, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082180, Length=475, Percent_Identity=32.8421052631579, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788468, Length=453, Percent_Identity=29.5805739514349, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789243, Length=445, Percent_Identity=28.7640449438202, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=510, Percent_Identity=41.9607843137255, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71984108, Length=470, Percent_Identity=25.7446808510638, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320030, Length=505, Percent_Identity=46.1386138613861, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI28574881, Length=507, Percent_Identity=48.9151873767258, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665539, Length=507, Percent_Identity=48.9151873767258, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665547, Length=507, Percent_Identity=48.9151873767258, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665543, Length=507, Percent_Identity=48.9151873767258, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24640763, Length=477, Percent_Identity=24.1090146750524, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18858217, Length=477, Percent_Identity=24.1090146750524, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53128; Mature: 52997

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTGFMEHGRIEEGHQSVAERLRHYKEFVIGLDEDQARVQGARCMDCGTPFCSHGCPV
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCHHCCCCCC
NNIIPDFNDLIYQADWKGALEVLHSTNNFPEFTGRICPAPCEAACVLNVNDKAVGIKSIE
CCCCCCHHHHEEECCCHHHHHHHHCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
HAIIDRAWQQGWVQPRPARHPSGKKVAVVGSGPAGMAAAQQLARAGHGVTLFEKNDRVGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKMEKSHIDRRIAQLQAEGVVLRPGVFVGSASEGLGKGSKVGNAAKETVTPE
EEECCCCCCEECHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCCCCCCCHHHHCCCHH
QLYAQFDAVLLTGGAEQSRDLPVPGRGLDGIHFAMEFLPQQNKVNAGDKLKGQLRADGKH
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCE
VIVIGGGDTGSDCVGTSRRHGAASVTQFELLPRPPEEENRPLTWPYWPIKLRTSSSHEEG
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCEEEEEECCCCCCHH
CAREFAIATREFVGDKGRVTGLKTVRVQFKDGKLVEIAGSEQTLPADLVLLAMGFVGPVA
HHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
PVLDAFGVEQDARGNARASTEPDGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAAHAV
HHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEECCCHHHHHHH
DRFLMGHSELPR
HHHHHCCCCCCC
>Mature Secondary Structure 
GKTTGFMEHGRIEEGHQSVAERLRHYKEFVIGLDEDQARVQGARCMDCGTPFCSHGCPV
CCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCHHCCCCCC
NNIIPDFNDLIYQADWKGALEVLHSTNNFPEFTGRICPAPCEAACVLNVNDKAVGIKSIE
CCCCCCHHHHEEECCCHHHHHHHHCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
HAIIDRAWQQGWVQPRPARHPSGKKVAVVGSGPAGMAAAQQLARAGHGVTLFEKNDRVGG
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKMEKSHIDRRIAQLQAEGVVLRPGVFVGSASEGLGKGSKVGNAAKETVTPE
EEECCCCCCEECHHHHHHHHHHHHHCCEEECCCEEEECCCCCCCCCCCCCCHHHHCCCHH
QLYAQFDAVLLTGGAEQSRDLPVPGRGLDGIHFAMEFLPQQNKVNAGDKLKGQLRADGKH
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEECCCCE
VIVIGGGDTGSDCVGTSRRHGAASVTQFELLPRPPEEENRPLTWPYWPIKLRTSSSHEEG
EEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEECCCEEEEEECCCCCCHH
CAREFAIATREFVGDKGRVTGLKTVRVQFKDGKLVEIAGSEQTLPADLVLLAMGFVGPVA
HHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHH
PVLDAFGVEQDARGNARASTEPDGGYATNVPKVFAAGDMRRGQSLVVWAIREGRQAAHAV
HHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEECCCHHHHHHH
DRFLMGHSELPR
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]