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The map label for this gene is hsaB [H]

Identifier: 121639487

GI number: 121639487

Start: 3987611

End: 3988174

Strand: Reverse

Name: hsaB [H]

Synonym: BCG_3632c

Alternate gene names: 121639487

Gene position: 3988174-3987611 (Counterclockwise)

Preceding gene: 121639488

Following gene: 121639486

Centisome position: 91.17

GC content: 63.83

Gene sequence:

>564_bases
ATGTCGGCTCAGATCGATCCACGCACGTTCCGCTCCGTGCTCGGTCAGTTCTGTACGGGGATCACTGTCATCACCACTGT
GCACGACGACGTGCCCGTCGGGTTTGCGTGCCAGTCGTTCGCGGCGCTGTCGTTGGAACCGCCGTTGGTGCTGTTCTGCC
CCACTAAGGTGTCGCGGTCCTGGCAGGCCATCGAGGCCAGCGGCCGGTTCTGCGTCAACGTACTGACCGAAAAGCAGAAA
GACGTCTCGGCGCGATTCGGCTCGAAGGAGCCGGACAAGTTCGCCGGTATCGATTGGCGCCCTTCGGAACTAGGCTCACC
GATCATCGAGGGATCGCTGGCCTACATTGACTGCACGGTGGCGTCGGTGCACGACGGCGGCGATCACTTCGTGGTGTTCG
GTGCCGTCGAGTCACTGTCAGAGGTCCCGGCGGTCAAGCCGCGGCCGCTGCTGTTCTATCGCGGCGACTACACCGGCATC
GAGCCGGAAAAGACCACGCCGGCGCACTGGCGCGACGACCTGGAGGCTTTCCTCACCACCACCACCCAGGACACCTGGCT
CTAG

Upstream 100 bases:

>100_bases
AAGTCGACGATCGGGACTGGATCGCCCGGGAGAGTACTGCGGTCAGCCTATGGGGTCACGATTTCACCGTAGGCGCTCGC
GGCTAGCTCGAAAGCAAACG

Downstream 100 bases:

>100_bases
TGGCGCGAGCAGACGCAAAATCGCCCATTTTGCGGGCGTGTTGGGCGATTTTGCGTCTGCTCGCCGTCGCCGGTAAGCAG
TCTGGTGAGCCACGTCCGGT

Product: putative oxidoreductase

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAIEASGRFCVNVLTEKQK
DVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGI
EPEKTTPAHWRDDLEAFLTTTTQDTWL

Sequences:

>Translated_187_residues
MSAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAIEASGRFCVNVLTEKQK
DVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGI
EPEKTTPAHWRDDLEAFLTTTTQDTWL
>Mature_186_residues
SAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRSWQAIEASGRFCVNVLTEKQKD
VSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTVASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGIE
PEKTTPAHWRDDLEAFLTTTTQDTWL

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=158, Percent_Identity=27.2151898734177, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 20527; Mature: 20396

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRS
CCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEEEECCCCEEEECCCCCCCC
WQAIEASGRFCVNVLTEKQKDVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTV
HHEECCCCCEEEEEHHHCHHHHHHHCCCCCCCHHCCCCCCHHHHCCHHHCCCEEEEEEEE
ASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGIEPEKTTPAHWRDDLEAFLTT
EEEECCCCEEEEEEEHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
TTQDTWL
CCCCCCC
>Mature Secondary Structure 
SAQIDPRTFRSVLGQFCTGITVITTVHDDVPVGFACQSFAALSLEPPLVLFCPTKVSRS
CCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHEEEECCCCEEEECCCCCCCC
WQAIEASGRFCVNVLTEKQKDVSARFGSKEPDKFAGIDWRPSELGSPIIEGSLAYIDCTV
HHEECCCCCEEEEEHHHCHHHHHHHCCCCCCCHHCCCCCCHHHHCCHHHCCCEEEEEEEE
ASVHDGGDHFVVFGAVESLSEVPAVKPRPLLFYRGDYTGIEPEKTTPAHWRDDLEAFLTT
EEEECCCCEEEEEEEHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHC
TTQDTWL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]