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The map label for this gene is nuoF

Identifier: 121639033

GI number: 121639033

Start: 3468459

End: 3469796

Strand: Direct

Name: nuoF

Synonym: BCG_3173

Alternate gene names: 121639033

Gene position: 3468459-3469796 (Clockwise)

Preceding gene: 121639032

Following gene: 121639034

Centisome position: 79.29

GC content: 65.92

Gene sequence:

>1338_bases
ATGACCACGCAGGCCACCCCGTTGACCCCGGTGATCAGCCGCCACTGGGACGACCCGGAGTCGTGGACCCTGGCCACTTA
TCAACGCCACGATCGCTATCGGGGCTATCAGGCGTTGCAGAAAGCCCTGACGATGCCGCCCGACGACGTGATCAGCATCG
TCAAGGATTCCGGGTTACGCGGACGCGGCGGCGCGGGCTTTGCCACCGGGACCAAGTGGTCGTTCATCCCGCAGGGCGAC
ACCGGCGCCGCGGCCAAGCCGCACTACCTGGTGGTCAACGCCGACGAGTCCGAACCCGGTACGTGCAAAGACATTCCGTT
GATGCTGGCGACGCCACATGTGCTCATCGAAGGCGTCATCATCGCCGCCTACGCGATCCGCGCCCATCACGCGTTCGTCT
ACGTACGCGGTGAGGTGGTGCCGGTATTGCGCCGGCTGCACAACGCGGTGGCCGAGGCCTATGCCGCCGGCTTCCTAGGC
CGCAACATCGGAGGTTCCGGATTCGATCTGGAGCTGGTGGTACACGCCGGCGCGGGCGCCTACATCTGCGGCGAGGAGAC
CGCCCTGCTCGACTCGCTGGAAGGCCGGCGCGGCCAGCCGCGGCTGCGGCCCCCCTTCCCCGCGGTGGCCGGTCTGTATG
GCTGCCCGACCGTGATCAACAACGTCGAAACGATCGCCAGTGTCCCATCGATCATCCTGGGCGGCATCGACTGGTTCCGG
TCGATGGGCAGCGAGAAATCGCCTGGCTTCACCCTGTATTCGCTGTCCGGCCACGTCACCCGCCCCGGCCAGTACGAGGC
GCCGCTGGGCATTACGCTGCGCGAGTTGCTCGACTACGCAGGCGGGGTGCGCGCCGGGCACCGGCTGAAGTTCTGGACAC
CGGGCGGCTCGTCGACCCCGCTGCTCACCGACGAGCATCTGGATGTGCCGCTGGACTACGAGGGTGTGGGTGCGGCCGGC
TCGATGCTGGGGACCAAGGCGCTGGAGATCTTCGACGAGACCACCTGCGTGGTGCGCGCGGTGCGCCGCTGGACCGAGTT
CTACAAGCACGAATCGTGTGGGAAATGCACGCCGTGCCGGGAGGGCACCTTCTGGCTGGATAAGATCTACGAGCGGCTGG
AAACCGGCCGGGGTAGCCATGAAGACATTGACAAACTGTTGGACATTTCCGATTCCATCTTGGGAAAGTCGTTCTGCGCG
TTGGGCGACGGTGCCGCGAGTCCGGTGATGTCGTCGATCAAGCACTTCCGCGACGAGTACCTGGCCCACGTCGAAGGAGG
CGGTTGCCCATTCGACCCCCGAGACTCCATGCTCGTCGCGAACGGAGTGGACGCGTGA

Upstream 100 bases:

>100_bases
CCGACGAAGGCCAGGGCGGTCCCGGCGCGCCCACCCTGGCCGGGCTGCAGGTGGCAAGGAAGAACGACATGCAGGCGCCA
CCAACCCCCGGAGCGGACGA

Downstream 100 bases:

>100_bases
CCCAGGCGGCCGACACTGACATCCGGGTAGGCCAACCGGAGATGGTGACACTGACCATCGACGGCGTCGAAATCAGCGTC
CCCAAGGGCACGTTGGTGAT

Product: putative NADH dehydrogenase I (chain F) nuoF

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTQATPLTPVISRHWDDPESWTLATYQRHDRYRGYQALQKALTMPPDDVISIVKDSGLRGRGGAGFATGTKWSFIPQGD
TGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVIIAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLG
RNIGGSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVPSIILGGIDWFR
SMGSEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLDYAGGVRAGHRLKFWTPGGSSTPLLTDEHLDVPLDYEGVGAAG
SMLGTKALEIFDETTCVVRAVRRWTEFYKHESCGKCTPCREGTFWLDKIYERLETGRGSHEDIDKLLDISDSILGKSFCA
LGDGAASPVMSSIKHFRDEYLAHVEGGGCPFDPRDSMLVANGVDA

Sequences:

>Translated_445_residues
MTTQATPLTPVISRHWDDPESWTLATYQRHDRYRGYQALQKALTMPPDDVISIVKDSGLRGRGGAGFATGTKWSFIPQGD
TGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVIIAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLG
RNIGGSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVPSIILGGIDWFR
SMGSEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLDYAGGVRAGHRLKFWTPGGSSTPLLTDEHLDVPLDYEGVGAAG
SMLGTKALEIFDETTCVVRAVRRWTEFYKHESCGKCTPCREGTFWLDKIYERLETGRGSHEDIDKLLDISDSILGKSFCA
LGDGAASPVMSSIKHFRDEYLAHVEGGGCPFDPRDSMLVANGVDA
>Mature_444_residues
TTQATPLTPVISRHWDDPESWTLATYQRHDRYRGYQALQKALTMPPDDVISIVKDSGLRGRGGAGFATGTKWSFIPQGDT
GAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVIIAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLGR
NIGGSGFDLELVVHAGAGAYICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVPSIILGGIDWFRS
MGSEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLDYAGGVRAGHRLKFWTPGGSSTPLLTDEHLDVPLDYEGVGAAGS
MLGTKALEIFDETTCVVRAVRRWTEFYKHESCGKCTPCREGTFWLDKIYERLETGRGSHEDIDKLLDISDSILGKSFCAL
GDGAASPVMSSIKHFRDEYLAHVEGGGCPFDPRDSMLVANGVDA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family

Homologues:

Organism=Homo sapiens, GI20149568, Length=403, Percent_Identity=47.394540942928, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI260656005, Length=403, Percent_Identity=47.394540942928, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1788620, Length=424, Percent_Identity=47.6415094339623, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17531727, Length=403, Percent_Identity=46.4019851116625, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI19920794, Length=416, Percent_Identity=47.3557692307692, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45550452, Length=400, Percent_Identity=46.75, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24653819, Length=412, Percent_Identity=36.4077669902913, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24653817, Length=349, Percent_Identity=35.8166189111748, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOF_MYCBO (P65568)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_856819.1
- ProteinModelPortal:   P65568
- EnsemblBacteria:   EBMYCT00000018131
- GeneID:   1093500
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3174
- GeneTree:   EBGT00050000016197
- HOGENOM:   HBG669338
- OMA:   HFYMHES
- ProtClustDB:   CLSK872146
- BioCyc:   MBOV233413:MB3174-MONOMER
- BRENDA:   1.6.99.5
- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554
- SMART:   SM00928
- TIGRFAMs:   TIGR01959

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB

EC number: =1.6.99.5

Molecular weight: Translated: 48102; Mature: 47971

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00644 COMPLEX1_51K_1; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQATPLTPVISRHWDDPESWTLATYQRHDRYRGYQALQKALTMPPDDVISIVKDSGLR
CCCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
GRGGAGFATGTKWSFIPQGDTGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVI
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEHCCHHHHHHHHH
IAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLGRNIGGSGFDLELVVHAGAGA
HHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
YICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVPSIILGGIDWFR
EEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMGSEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLDYAGGVRAGHRLKFWTPGGSSTP
HCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
LLTDEHLDVPLDYEGVGAAGSMLGTKALEIFDETTCVVRAVRRWTEFYKHESCGKCTPCR
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGTFWLDKIYERLETGRGSHEDIDKLLDISDSILGKSFCALGDGAASPVMSSIKHFRDEY
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LAHVEGGGCPFDPRDSMLVANGVDA
HHHCCCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
TTQATPLTPVISRHWDDPESWTLATYQRHDRYRGYQALQKALTMPPDDVISIVKDSGLR
CCCCCCCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
GRGGAGFATGTKWSFIPQGDTGAAAKPHYLVVNADESEPGTCKDIPLMLATPHVLIEGVI
CCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCEEHCCHHHHHHHHH
IAAYAIRAHHAFVYVRGEVVPVLRRLHNAVAEAYAAGFLGRNIGGSGFDLELVVHAGAGA
HHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCE
YICGEETALLDSLEGRRGQPRLRPPFPAVAGLYGCPTVINNVETIASVPSIILGGIDWFR
EEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SMGSEKSPGFTLYSLSGHVTRPGQYEAPLGITLRELLDYAGGVRAGHRLKFWTPGGSSTP
HCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
LLTDEHLDVPLDYEGVGAAGSMLGTKALEIFDETTCVVRAVRRWTEFYKHESCGKCTPCR
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGTFWLDKIYERLETGRGSHEDIDKLLDISDSILGKSFCALGDGAASPVMSSIKHFRDEY
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LAHVEGGGCPFDPRDSMLVANGVDA
HHHCCCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972