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The map label for this gene is tgs4

Identifier: 121638973

GI number: 121638973

Start: 3408826

End: 3410250

Strand: Direct

Name: tgs4

Synonym: BCG_3113

Alternate gene names: 121638973

Gene position: 3408826-3410250 (Clockwise)

Preceding gene: 121638972

Following gene: 121638974

Centisome position: 77.92

GC content: 65.89

Gene sequence:

>1425_bases
ATGACCCGCATCAATCCGATCGATCTGTCCTTCCTGCTGCTGGAGCGGGCCAACCGGCCCAACCACATGGCCGCCTACAC
GATCTTCGAAAAGCCGAAAGGACAGAAATCGTCGTTCGGGCCGCGCCTGTTCGATGCCTACCGGCACAGCCAGGCGGCCA
AGCCCTTCAATCACAAGCTGAAATGGCTGGGCACAGATGTTGCGGCGTGGGAAACCGTCGAGCCCGACATGGGCTATCAC
ATTCGACACCTCGCCCTGCCCGCACCGGGTTCCATGCAGCAGTTCCACGAAACGGTCTCGTTCCTCAACACCGGCCTGCT
CGATAGGGGCCACCCGATGTGGGAGTGCTACATCATCGACGGCATCGAGCGCGGCCGGATCGCGATCCTGCTCAAGGTGC
ACCACGCGCTCATCGACGGTGAAGGCGGCCTGCGCGCGATGCGCAACTTCCTCTCCGATTCACCGGACGACACGACGCTG
GCCGGTCCCTGGATGTCGGCGCAGGGCGCCGACCGGCCACGGCGCACCCCCGCCACGGTGTCGCGCAGGGCGCAACTGCA
AGGACAACTGCAAGGAATGATCAAGGGGCTGACCAAGCTGCCGAGCGGCCTGTTCGGCGTCAGCGCGGACGCGGCGGACC
TTGGTGCGCAGGCACTGAGCCTCAAGGCGCGCAAGGCGTCCCTGCCCTTCACGGCGCGACGCACTCTGTTCAACAACACG
GCGAAATCGGCGGCGCGCGCGTACGGGAACGTCGAGTTGCCGCTCGCCGACGTCAAGGCCCTGGCCAAGGCGACCGGCAC
CTCGGTCAACGACGTGGTGATGACGGTCATCGACGACGCGCTGCACCACTACCTCGCCGAACACCAGGCGTCCACCGACC
GGCCGCTGGTGGCGTTCATGCCGATGTCGCTGCGTGAGAAGTCGGGCGAGGGCGGTGGCAACCGGGTGAGCGCCGAACTG
GTCCCGATGGGTGCACCCAAGGCGAGTCCCGTTGAGCGCCTTAAGGAAATCAACGCGGCGACCACACGCGCGAAGGACAA
AGGGCGCGGCATGCAAACGACGTCCCGCCAGGCCTACGCGCTGCTACTGCTCGGCAGCCTGACGGTGGCGGACGCCCTGC
CCCTGCTCGGCAAGTTGCCGAGCGCGAATGTGGTGATATCAAACATGAAGGGGCCCACCGAGCAGCTCTACCTTGCCGGT
GCGCCGCTGGTGGCGTTCAGTGGCCTGCCCATCGTGCCGCCGGGCGCCGGGCTTAACGTCACCTTCGCCAGCATCAACAC
CGCGCTGTGCATCGCCATCGGCGCGGCACCGGAAGCCGTGCACGAACCCTCCCGGCTGGCCGAACTCATGCAACGGGCAT
TCACCGAGCTCCAAACCGAAGCCGGCACAACGAGTCCCACAACATCGAAGTCGAGAACCCCATGA

Upstream 100 bases:

>100_bases
CGGTTCGGAACACCTGGGAATTGGTCGGCGGCTTCCGCGCCTCGCACGAGGAGCTGCTCGCGGCGGCCCGTGCCCAAGCC
ACGCCCAAGGAGATGGCCAC

Downstream 100 bases:

>100_bases
AGAACATTGGCTGGATGCTCAGACAACGCGCGACCGTCTCGCCGCGGCTGCAAGCCTACGTCGAGCCGTCCACCGACGTC
CGGATGACCTACGCGCAGAT

Product: hypothetical protein

Products: NA

Alternate protein names: TGS4; Probable triacylglycerol synthase tgs4

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTRINPIDLSFLLLERANRPNHMAAYTIFEKPKGQKSSFGPRLFDAYRHSQAAKPFNHKLKWLGTDVAAWETVEPDMGYH
IRHLALPAPGSMQQFHETVSFLNTGLLDRGHPMWECYIIDGIERGRIAILLKVHHALIDGEGGLRAMRNFLSDSPDDTTL
AGPWMSAQGADRPRRTPATVSRRAQLQGQLQGMIKGLTKLPSGLFGVSADAADLGAQALSLKARKASLPFTARRTLFNNT
AKSAARAYGNVELPLADVKALAKATGTSVNDVVMTVIDDALHHYLAEHQASTDRPLVAFMPMSLREKSGEGGGNRVSAEL
VPMGAPKASPVERLKEINAATTRAKDKGRGMQTTSRQAYALLLLGSLTVADALPLLGKLPSANVVISNMKGPTEQLYLAG
APLVAFSGLPIVPPGAGLNVTFASINTALCIAIGAAPEAVHEPSRLAELMQRAFTELQTEAGTTSPTTSKSRTP

Sequences:

>Translated_474_residues
MTRINPIDLSFLLLERANRPNHMAAYTIFEKPKGQKSSFGPRLFDAYRHSQAAKPFNHKLKWLGTDVAAWETVEPDMGYH
IRHLALPAPGSMQQFHETVSFLNTGLLDRGHPMWECYIIDGIERGRIAILLKVHHALIDGEGGLRAMRNFLSDSPDDTTL
AGPWMSAQGADRPRRTPATVSRRAQLQGQLQGMIKGLTKLPSGLFGVSADAADLGAQALSLKARKASLPFTARRTLFNNT
AKSAARAYGNVELPLADVKALAKATGTSVNDVVMTVIDDALHHYLAEHQASTDRPLVAFMPMSLREKSGEGGGNRVSAEL
VPMGAPKASPVERLKEINAATTRAKDKGRGMQTTSRQAYALLLLGSLTVADALPLLGKLPSANVVISNMKGPTEQLYLAG
APLVAFSGLPIVPPGAGLNVTFASINTALCIAIGAAPEAVHEPSRLAELMQRAFTELQTEAGTTSPTTSKSRTP
>Mature_473_residues
TRINPIDLSFLLLERANRPNHMAAYTIFEKPKGQKSSFGPRLFDAYRHSQAAKPFNHKLKWLGTDVAAWETVEPDMGYHI
RHLALPAPGSMQQFHETVSFLNTGLLDRGHPMWECYIIDGIERGRIAILLKVHHALIDGEGGLRAMRNFLSDSPDDTTLA
GPWMSAQGADRPRRTPATVSRRAQLQGQLQGMIKGLTKLPSGLFGVSADAADLGAQALSLKARKASLPFTARRTLFNNTA
KSAARAYGNVELPLADVKALAKATGTSVNDVVMTVIDDALHHYLAEHQASTDRPLVAFMPMSLREKSGEGGGNRVSAELV
PMGAPKASPVERLKEINAATTRAKDKGRGMQTTSRQAYALLLLGSLTVADALPLLGKLPSANVVISNMKGPTEQLYLAGA
PLVAFSGLPIVPPGAGLNVTFASINTALCIAIGAAPEAVHEPSRLAELMQRAFTELQTEAGTTSPTTSKSRTP

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGS4_MYCTU (P67208)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   D70853
- RefSeq:   NP_217604.1
- RefSeq:   NP_337695.1
- ProteinModelPortal:   P67208
- EnsemblBacteria:   EBMYCT00000003403
- EnsemblBacteria:   EBMYCT00000069262
- GeneID:   888669
- GeneID:   926669
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3173
- KEGG:   mtu:Rv3088
- TIGR:   MT3173
- TubercuList:   Rv3088
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG569259
- OMA:   AYANMEL
- ProtClustDB:   CLSK792273
- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 50887; Mature: 50756

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRINPIDLSFLLLERANRPNHMAAYTIFEKPKGQKSSFGPRLFDAYRHSQAAKPFNHKL
CCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHCCH
KWLGTDVAAWETVEPDMGYHIRHLALPAPGSMQQFHETVSFLNTGLLDRGHPMWECYIID
HEECCCHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GIERGRIAILLKVHHALIDGEGGLRAMRNFLSDSPDDTTLAGPWMSAQGADRPRRTPATV
CCCCCCEEEEEEEHHHHHCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCHHH
SRRAQLQGQLQGMIKGLTKLPSGLFGVSADAADLGAQALSLKARKASLPFTARRTLFNNT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHHHHHHHHCCCCHHHHHHHHHHH
AKSAARAYGNVELPLADVKALAKATGTSVNDVVMTVIDDALHHYLAEHQASTDRPLVAFM
HHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PMSLREKSGEGGGNRVSAELVPMGAPKASPVERLKEINAATTRAKDKGRGMQTTSRQAYA
CCHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHEE
LLLLGSLTVADALPLLGKLPSANVVISNMKGPTEQLYLAGAPLVAFSGLPIVPPGAGLNV
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHEEECCCCHHHCCCCCCCCCCCCCEE
TFASINTALCIAIGAAPEAVHEPSRLAELMQRAFTELQTEAGTTSPTTSKSRTP
EEEECCEEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRINPIDLSFLLLERANRPNHMAAYTIFEKPKGQKSSFGPRLFDAYRHSQAAKPFNHKL
CCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHCCH
KWLGTDVAAWETVEPDMGYHIRHLALPAPGSMQQFHETVSFLNTGLLDRGHPMWECYIID
HEECCCHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GIERGRIAILLKVHHALIDGEGGLRAMRNFLSDSPDDTTLAGPWMSAQGADRPRRTPATV
CCCCCCEEEEEEEHHHHHCCCCCHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCHHH
SRRAQLQGQLQGMIKGLTKLPSGLFGVSADAADLGAQALSLKARKASLPFTARRTLFNNT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHHHHHHHHCCCCHHHHHHHHHHH
AKSAARAYGNVELPLADVKALAKATGTSVNDVVMTVIDDALHHYLAEHQASTDRPLVAFM
HHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PMSLREKSGEGGGNRVSAELVPMGAPKASPVERLKEINAATTRAKDKGRGMQTTSRQAYA
CCHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCCHHHEE
LLLLGSLTVADALPLLGKLPSANVVISNMKGPTEQLYLAGAPLVAFSGLPIVPPGAGLNV
HHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHEEECCCCHHHCCCCCCCCCCCCCEE
TFASINTALCIAIGAAPEAVHEPSRLAELMQRAFTELQTEAGTTSPTTSKSRTP
EEEECCEEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036