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The map label for this gene is fadE22 [H]

Identifier: 121638946

GI number: 121638946

Start: 3377748

End: 3379913

Strand: Reverse

Name: fadE22 [H]

Synonym: BCG_3086c

Alternate gene names: 121638946

Gene position: 3379913-3377748 (Counterclockwise)

Preceding gene: 121638949

Following gene: 121638945

Centisome position: 77.26

GC content: 66.07

Gene sequence:

>2166_bases
ATGGGTATTGCATTGACCGACGACCATCGCGAGCTCTCCGGGGTGGCTCGCGCGTTCTTGACTTCGCAGAAGGTGCGCTG
GGCGGCGCGTGCATCACTGGACGCGGCGGGGGACGCCCGCCCGCCGTTCTGGCAGAACCTCGCCGAGCTGGGCTGGCTCG
GCCTGCATATCGACGAGCGACACGGTGGCTCTGGCTATGGCCTGTCCGAGCTTGTGGTGGTGATCGAAGAGCTCGGTCGT
GCGGTGGCACCGGGGCTGTTTGTGCCGACCGTGATCGCCTCAGCGGTGGTCGCCAAAGAAGGTACTGATGACCAACGGGC
ACGGCTGTTGCCGGCGCTGATTGACGGAACCCTGACGGCGGGTGTGGGACTGGATAGTCAGGTGCAGGTTACCGACGGTG
TTGCCGACGGTGAGGCGGGAATCGTGTTGGGCGCCGGGCTTGCCGAGCTGCTGTTGGTTGCCGCCGGTGACGACGTGCTG
GTGTTGGAACGCGGCCGCAAGGGCGTCTCGGTTGATGTGCCGGAAAACTTTGATCCGACCCGGCGGAGTGGCCGCGTGCG
CCTGGACAACGTGCGCGTCACGACCGACGACATCCTGCTTGGTGCGTATGAATCGGCTTTGGCCCGCGCGCGCACATTGC
TGGCCGCCGAGGCCGTCGGTGGGGCGGCCGACTGCGTGGACAGCGCCGTGGCCTATGCCAAGGTGCGACAGCAATTCGGC
CGTACCATCGCCACGTTTCAAGCGGTGAAGCATCACTGCGCGAACATGCTGGTGGCCGCCGAGTCGGCGATCGCCGCGGT
CTGGGATGCCGCGCGTGCGGCAGCAGAGGATGAGGAGCAGTTTCGGCTGGCCGCCGCGGTCGCTGCGGCCCTGGCGTTTC
CGGCCTATGCACGCAATGCCGAGCTCAACATCCAGGTGCACGGCGGTATTGGCTTTACCTGGGAGCATGACGCGCATCTG
CATCTGCGCCGGGCGTTGGTGACCGTGGGATTGTTCGGCGGTGATGCGCCCGTCCGAGACGTTTTCGAGCGCACCGCGGC
TGGCGTTACCCGGGCGATCAGCTTGGACCTGCCGGCACAGGCCGAGGAGCTGCGCGCCCGCATCCGTTCGGACGCCGCTG
AAATCGCTGCTCTGGAAAAGGATGCACAGCGCGACAAGCTGATCGAGACGGGCTATGTGATGCCGCATTGGCCCAGGCCG
TGGGGTCGTGCCGCGGGCGCGGTGGAGCAGTTGGTGATCGAGGAAGAGTTCAGCGCGGCGGGCATCGAGCGCCCGGATTA
CTCGATCACCGGGTGGGTGATCTTGACGCTGATTCAGCACGGAACGCCTTGGCAGATTGAAAGATTCGTCGAGAAAGCGC
TGCGCCAGCAGGAGATATGGTGCCAACTGTTCTCCGAACCTGACGCGGGGTCTGACGCGGCCTCGGTCAAGACCCGCGCA
ACTCGGGTGGAGGGCGGCTGGGAGATCAACGGGCAAAAGGTGTGGACCTGCGGAGCGCAGTACTGCGCGCGTGGCCTGGC
TACCGTGCGTACCGATCCGGATGCCCCCAAACACGCTGGCATCACCACGGTGATCATCGACATGTTGGCCCCCGGTGTCG
AGGTGCGGCCGCTGCGGCAGATCACCGGCGACTCGGAATTCAACGAGGTGTTCTTCAACGATGTCTTCGTCCCCGATGAG
GACGTTGTCGGGGCGCCGAACTCCGGGTGGACGGTGGCGCGGGCAACGCTGGGCAACGAGCGGGTCAGCATCGGCGGCAG
TGGCTCGTACTACGAAGCAATGGCGGCGAAGCTGGTGCAATTGGTCCAGCGGCGGTCAGATGCGTTTGCGGGCGCCCCAA
TTCGAGTCGGCGCTTTCCTCGCAGAGGATCACGCACTGCGGCTGCTGAACCTGCGCCGTGCCGCTCGCAGCGTCGAAGGA
GCCGGCCCTGGTCCGGAGGGCAACATCACCAAGCTCAAAGTGGCAGAGCACATGATCGAGGGCGCCGCCATCGCGGCCGC
GCTATGGGGGCCCGAGATTGCGTTGCTGGACGGCCCCGGCAGGGTGATTGGCCGAACGGTGATGGGCGCCCGTGGCATGG
CGATCGCCGGCGGCACGTCGGAGGTGACCCGCAATCAGATTGCCGAGCGGATCCTGGGCATGCCGCGTGATCCCCTGATT
AGCTAG

Upstream 100 bases:

>100_bases
GAGCAAGCCGTGTCTCGCGGCCGCGACAGACAATTAGTATACTCTTTACCAATACCGCCTGGGGGCTATGTGGGTCCGGT
AGTGCCGCTAGGAGGCCTTG

Downstream 100 bases:

>100_bases
GGCCCAGCTCGCCCGGTTGCGCGAGCAGACGCAGAATCGCCTCAAAATCTTGGATTTTGGGCGATTCTGCGTCTGCTCGC
GCCCTTGACTCAGCCAATAC

Product: putative acyl-CoA dehydrogenase fadE22

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MGIALTDDHRELSGVARAFLTSQKVRWAARASLDAAGDARPPFWQNLAELGWLGLHIDERHGGSGYGLSELVVVIEELGR
AVAPGLFVPTVIASAVVAKEGTDDQRARLLPALIDGTLTAGVGLDSQVQVTDGVADGEAGIVLGAGLAELLLVAAGDDVL
VLERGRKGVSVDVPENFDPTRRSGRVRLDNVRVTTDDILLGAYESALARARTLLAAEAVGGAADCVDSAVAYAKVRQQFG
RTIATFQAVKHHCANMLVAAESAIAAVWDAARAAAEDEEQFRLAAAVAAALAFPAYARNAELNIQVHGGIGFTWEHDAHL
HLRRALVTVGLFGGDAPVRDVFERTAAGVTRAISLDLPAQAEELRARIRSDAAEIAALEKDAQRDKLIETGYVMPHWPRP
WGRAAGAVEQLVIEEEFSAAGIERPDYSITGWVILTLIQHGTPWQIERFVEKALRQQEIWCQLFSEPDAGSDAASVKTRA
TRVEGGWEINGQKVWTCGAQYCARGLATVRTDPDAPKHAGITTVIIDMLAPGVEVRPLRQITGDSEFNEVFFNDVFVPDE
DVVGAPNSGWTVARATLGNERVSIGGSGSYYEAMAAKLVQLVQRRSDAFAGAPIRVGAFLAEDHALRLLNLRRAARSVEG
AGPGPEGNITKLKVAEHMIEGAAIAAALWGPEIALLDGPGRVIGRTVMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
S

Sequences:

>Translated_721_residues
MGIALTDDHRELSGVARAFLTSQKVRWAARASLDAAGDARPPFWQNLAELGWLGLHIDERHGGSGYGLSELVVVIEELGR
AVAPGLFVPTVIASAVVAKEGTDDQRARLLPALIDGTLTAGVGLDSQVQVTDGVADGEAGIVLGAGLAELLLVAAGDDVL
VLERGRKGVSVDVPENFDPTRRSGRVRLDNVRVTTDDILLGAYESALARARTLLAAEAVGGAADCVDSAVAYAKVRQQFG
RTIATFQAVKHHCANMLVAAESAIAAVWDAARAAAEDEEQFRLAAAVAAALAFPAYARNAELNIQVHGGIGFTWEHDAHL
HLRRALVTVGLFGGDAPVRDVFERTAAGVTRAISLDLPAQAEELRARIRSDAAEIAALEKDAQRDKLIETGYVMPHWPRP
WGRAAGAVEQLVIEEEFSAAGIERPDYSITGWVILTLIQHGTPWQIERFVEKALRQQEIWCQLFSEPDAGSDAASVKTRA
TRVEGGWEINGQKVWTCGAQYCARGLATVRTDPDAPKHAGITTVIIDMLAPGVEVRPLRQITGDSEFNEVFFNDVFVPDE
DVVGAPNSGWTVARATLGNERVSIGGSGSYYEAMAAKLVQLVQRRSDAFAGAPIRVGAFLAEDHALRLLNLRRAARSVEG
AGPGPEGNITKLKVAEHMIEGAAIAAALWGPEIALLDGPGRVIGRTVMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
S
>Mature_720_residues
GIALTDDHRELSGVARAFLTSQKVRWAARASLDAAGDARPPFWQNLAELGWLGLHIDERHGGSGYGLSELVVVIEELGRA
VAPGLFVPTVIASAVVAKEGTDDQRARLLPALIDGTLTAGVGLDSQVQVTDGVADGEAGIVLGAGLAELLLVAAGDDVLV
LERGRKGVSVDVPENFDPTRRSGRVRLDNVRVTTDDILLGAYESALARARTLLAAEAVGGAADCVDSAVAYAKVRQQFGR
TIATFQAVKHHCANMLVAAESAIAAVWDAARAAAEDEEQFRLAAAVAAALAFPAYARNAELNIQVHGGIGFTWEHDAHLH
LRRALVTVGLFGGDAPVRDVFERTAAGVTRAISLDLPAQAEELRARIRSDAAEIAALEKDAQRDKLIETGYVMPHWPRPW
GRAAGAVEQLVIEEEFSAAGIERPDYSITGWVILTLIQHGTPWQIERFVEKALRQQEIWCQLFSEPDAGSDAASVKTRAT
RVEGGWEINGQKVWTCGAQYCARGLATVRTDPDAPKHAGITTVIIDMLAPGVEVRPLRQITGDSEFNEVFFNDVFVPDED
VVGAPNSGWTVARATLGNERVSIGGSGSYYEAMAAKLVQLVQRRSDAFAGAPIRVGAFLAEDHALRLLNLRRAARSVEGA
GPGPEGNITKLKVAEHMIEGAAIAAALWGPEIALLDGPGRVIGRTVMGARGMAIAGGTSEVTRNQIAERILGMPRDPLIS

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=356, Percent_Identity=26.685393258427, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI226958414, Length=320, Percent_Identity=26.875, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI226958412, Length=320, Percent_Identity=26.875, Blast_Score=80, Evalue=7e-15,
Organism=Homo sapiens, GI4501857, Length=362, Percent_Identity=23.2044198895028, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI187960098, Length=310, Percent_Identity=26.4516129032258, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI4557231, Length=310, Percent_Identity=26.4516129032258, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI4557233, Length=292, Percent_Identity=29.4520547945205, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1786223, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081958, Length=244, Percent_Identity=28.2786885245902, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17506239, Length=357, Percent_Identity=27.1708683473389, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17508101, Length=313, Percent_Identity=27.4760383386581, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990804, Length=315, Percent_Identity=26.3492063492064, Blast_Score=90, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17570075, Length=403, Percent_Identity=24.5657568238213, Blast_Score=83, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32563615, Length=231, Percent_Identity=27.7056277056277, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538396, Length=372, Percent_Identity=23.9247311827957, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17569725, Length=404, Percent_Identity=24.2574257425743, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17534899, Length=286, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666513, Length=352, Percent_Identity=28.4090909090909, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355753, Length=388, Percent_Identity=26.8041237113402, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24660351, Length=387, Percent_Identity=24.2894056847545, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363737, Length=161, Percent_Identity=31.055900621118, Blast_Score=73, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24646207, Length=283, Percent_Identity=26.8551236749117, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356377, Length=352, Percent_Identity=26.1363636363636, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 76759; Mature: 76628

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00482 DIHYDROOROTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIALTDDHRELSGVARAFLTSQKVRWAARASLDAAGDARPPFWQNLAELGWLGLHIDER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCEEEEEEECC
HGGSGYGLSELVVVIEELGRAVAPGLFVPTVIASAVVAKEGTDDQRARLLPALIDGTLTA
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHC
GVGLDSQVQVTDGVADGEAGIVLGAGLAELLLVAAGDDVLVLERGRKGVSVDVPENFDPT
CCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCC
RRSGRVRLDNVRVTTDDILLGAYESALARARTLLAAEAVGGAADCVDSAVAYAKVRQQFG
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RTIATFQAVKHHCANMLVAAESAIAAVWDAARAAAEDEEQFRLAAAVAAALAFPAYARNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
ELNIQVHGGIGFTWEHDAHLHLRRALVTVGLFGGDAPVRDVFERTAAGVTRAISLDLPAQ
EEEEEEECCEEEEECCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHEEEEEECCCCC
AEELRARIRSDAAEIAALEKDAQRDKLIETGYVMPHWPRPWGRAAGAVEQLVIEEEFSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GIERPDYSITGWVILTLIQHGTPWQIERFVEKALRQQEIWCQLFSEPDAGSDAASVKTRA
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TRVEGGWEINGQKVWTCGAQYCARGLATVRTDPDAPKHAGITTVIIDMLAPGVEVRPLRQ
EEECCCEEECCCEEEECCHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
ITGDSEFNEVFFNDVFVPDEDVVGAPNSGWTVARATLGNERVSIGGSGSYYEAMAAKLVQ
HCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
LVQRRSDAFAGAPIRVGAFLAEDHALRLLNLRRAARSVEGAGPGPEGNITKLKVAEHMIE
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHH
GAAIAAALWGPEIALLDGPGRVIGRTVMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
HHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCC
S
C
>Mature Secondary Structure 
GIALTDDHRELSGVARAFLTSQKVRWAARASLDAAGDARPPFWQNLAELGWLGLHIDER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCEEEEEEECC
HGGSGYGLSELVVVIEELGRAVAPGLFVPTVIASAVVAKEGTDDQRARLLPALIDGTLTA
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHC
GVGLDSQVQVTDGVADGEAGIVLGAGLAELLLVAAGDDVLVLERGRKGVSVDVPENFDPT
CCCCCCCEEEECCCCCCCCCEEEECCHHHHHHHCCCCCEEEEECCCCCCEEECCCCCCCC
RRSGRVRLDNVRVTTDDILLGAYESALARARTLLAAEAVGGAADCVDSAVAYAKVRQQFG
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RTIATFQAVKHHCANMLVAAESAIAAVWDAARAAAEDEEQFRLAAAVAAALAFPAYARNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
ELNIQVHGGIGFTWEHDAHLHLRRALVTVGLFGGDAPVRDVFERTAAGVTRAISLDLPAQ
EEEEEEECCEEEEECCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHEEEEEECCCCC
AEELRARIRSDAAEIAALEKDAQRDKLIETGYVMPHWPRPWGRAAGAVEQLVIEEEFSAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GIERPDYSITGWVILTLIQHGTPWQIERFVEKALRQQEIWCQLFSEPDAGSDAASVKTRA
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
TRVEGGWEINGQKVWTCGAQYCARGLATVRTDPDAPKHAGITTVIIDMLAPGVEVRPLRQ
EEECCCEEECCCEEEECCHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
ITGDSEFNEVFFNDVFVPDEDVVGAPNSGWTVARATLGNERVSIGGSGSYYEAMAAKLVQ
HCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHH
LVQRRSDAFAGAPIRVGAFLAEDHALRLLNLRRAARSVEGAGPGPEGNITKLKVAEHMIE
HHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHH
GAAIAAALWGPEIALLDGPGRVIGRTVMGARGMAIAGGTSEVTRNQIAERILGMPRDPLI
HHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]