BacMap

Definition	Mycobacterium bovis BCG str. Pasteur 1173P2, complete genome.
Accession	NC_008769
Length	4,374,522

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The map label for this gene is yqhE [C]

Identifier: 121638853

GI number: 121638853

Start: 3280263

End: 3281111

Strand: Direct

Name: yqhE [C]

Synonym: BCG_2993

Alternate gene names: 121638853

Gene position: 3280263-3281111 (Clockwise)

Preceding gene: 121638852

Following gene: 121638861

Centisome position: 74.99

GC content: 63.84

Gene sequence:

>849_bases
GTGACTGGCGAGTCGGGCGCCGCCGCCGCACCCTCGATTACCCTCAACGACGAGCATACGATGCCGGTGCTTGGCCTCGG
CGTCGCGGAATTGTCGGACGACGAGACCGAACGTGCGGTGTCCGCGGCGCTGGAAATTGGCTGCCGGCTGATCGACACCG
CCTACGCCTATGGCAACGAGGCCGCGGTCGGCCGCGCAATTGCAGCCTCCGGCGTTGCCCGCGAAGAGCTGTTCGTCACC
ACCAAGCTAGCCACCCCCGACCAGGGTTTCACCCGTTCCCAGGAAGCATGTAGAGCCAGTTTGGACCGCCTCGGCCTCGA
CTACGTCGACCTTTACCTAATTCACTGGCCGGCCCCGCCGGTGGGCAAGTATGTGGACGCCTGGGGAGGCATGATTCAAT
CCCGCGGAGAGGGCCATGCCCGATCGATCGGCGTGTCCAACTTCACCGCGGAGCACATCGAAAACCTTATCGACCTCACA
TTCGTCACGCCGGCGGTCAACCAGATCGAGCTGCACCCGCTGCTCAACCAGGACGAACTGCGCAAAGCTAACGCCCAGCA
CACCGTCGTCACACAGTCCTACTGCCCCCTGGCACTCGGCAGGCTGCTGGACAACCCAACCGTCACATCAATCGCCAGCG
AATACGTCAAGACGCCCGCACAAGTGCTGCTGCGGTGGAACCTGCAATTGGGCAATGCGGTGGTCGTCCGCTCGGCCAGA
CCCGAGCGCATCGCCAGCAACTTCGACGTCTTCGACTTCGAGTTGGCGGCCGAACACATGGATGCATTGGGCGGGCTCAA
TGACGGCACCCGGGTGCGCGAGGATCCACTGACCTACGCCGGCACCTGA

Upstream 100 bases:

>100_bases
TTGCGAGCAACTTCGACGCGTTCGATTTCCCTCGCTCGATGACGGAACCCGGCTTGGTCCGAATCCGAAAACCTTCAATT
TCACAGGCAGGTGAGATGAC

Downstream 100 bases:

>100_bases
TACGCCGCCGACTGTGAACCGCGCGACGTCTCCTCGGCGTGTCACGTCGTGAGATTCACCGTCGGCGCGTGGACTAGCCC
GTCGGGCAGGTGGCCGCGGC

Product: putative oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNEAAVGRAIAASGVAREELFVT
TKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPPVGKYVDAWGGMIQSRGEGHARSIGVSNFTAEHIENLIDLT
FVTPAVNQIELHPLLNQDELRKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT

Sequences:

>Translated_282_residues
MTGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNEAAVGRAIAASGVAREELFVT
TKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPPVGKYVDAWGGMIQSRGEGHARSIGVSNFTAEHIENLIDLT
FVTPAVNQIELHPLLNQDELRKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT
>Mature_281_residues
TGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNEAAVGRAIAASGVAREELFVTT
KLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPPVGKYVDAWGGMIQSRGEGHARSIGVSNFTAEHIENLIDLTF
VTPAVNQIELHPLLNQDELRKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSARP
ERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=296, Percent_Identity=33.7837837837838, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI5174391, Length=296, Percent_Identity=33.7837837837838, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=36.7857142857143, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=36.5771812080537, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI310109920, Length=298, Percent_Identity=36.241610738255, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI45446745, Length=298, Percent_Identity=36.241610738255, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI4503285, Length=298, Percent_Identity=36.241610738255, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=35.7859531772575, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI93277124, Length=306, Percent_Identity=34.3137254901961, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI223468663, Length=296, Percent_Identity=33.7837837837838, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4502049, Length=300, Percent_Identity=36.6666666666667, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI24497583, Length=294, Percent_Identity=34.6938775510204, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5174695, Length=311, Percent_Identity=33.4405144694534, Blast_Score=147, Evalue=9e-36,
Organism=Homo sapiens, GI291291012, Length=274, Percent_Identity=32.8467153284672, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI300116273, Length=287, Percent_Identity=33.1010452961672, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI300116271, Length=291, Percent_Identity=32.9896907216495, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=34.1935483870968, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI207028673, Length=112, Percent_Identity=44.6428571428571, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=44.6428571428571, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082198, Length=271, Percent_Identity=47.9704797047971, Blast_Score=262, Evalue=1e-71,
Organism=Escherichia coli, GI1786400, Length=257, Percent_Identity=38.1322957198444, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788081, Length=272, Percent_Identity=30.1470588235294, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788070, Length=205, Percent_Identity=27.8048780487805, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71998625, Length=267, Percent_Identity=36.3295880149813, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537077, Length=294, Percent_Identity=34.6938775510204, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537075, Length=294, Percent_Identity=35.0340136054422, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17552492, Length=277, Percent_Identity=35.0180505415162, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=33.1103678929766, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17566692, Length=290, Percent_Identity=32.7586206896552, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17561298, Length=277, Percent_Identity=32.4909747292419, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17550248, Length=297, Percent_Identity=34.3434343434343, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17537079, Length=301, Percent_Identity=29.9003322259136, Blast_Score=137, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17538386, Length=280, Percent_Identity=31.0714285714286, Blast_Score=133, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17561300, Length=279, Percent_Identity=31.1827956989247, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17562292, Length=278, Percent_Identity=29.136690647482, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6320576, Length=274, Percent_Identity=38.6861313868613, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324694, Length=288, Percent_Identity=36.8055555555556, Blast_Score=165, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6322556, Length=258, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=34.6938775510204, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321896, Length=315, Percent_Identity=30.1587301587302, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320079, Length=284, Percent_Identity=30.6338028169014, Blast_Score=127, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24663317, Length=297, Percent_Identity=37.7104377104377, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=38.2758620689655, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI20129731, Length=291, Percent_Identity=38.1443298969072, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=36.3333333333333, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21356425, Length=307, Percent_Identity=35.5048859934853, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=34.375, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI45553081, Length=296, Percent_Identity=34.7972972972973, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24662789, Length=298, Percent_Identity=34.2281879194631, Blast_Score=161, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24662785, Length=288, Percent_Identity=35.0694444444444, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=35, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=31.9230769230769, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 30387; Mature: 30256

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNE
CCCCCCCCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AAVGRAIAASGVAREELFVTTKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPP
HHHHHHHHHCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VGKYVDAWGGMIQSRGEGHARSIGVSNFTAEHIENLIDLTFVTPAVNQIELHPLLNQDEL
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
RKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
HHCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHEEECEEECCEEEEECCC
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT
CHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGESGAAAAPSITLNDEHTMPVLGLGVAELSDDETERAVSAALEIGCRLIDTAYAYGNE
CCCCCCCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
AAVGRAIAASGVAREELFVTTKLATPDQGFTRSQEACRASLDRLGLDYVDLYLIHWPAPP
HHHHHHHHHCCCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCC
VGKYVDAWGGMIQSRGEGHARSIGVSNFTAEHIENLIDLTFVTPAVNQIELHPLLNQDEL
HHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHH
RKANAQHTVVTQSYCPLALGRLLDNPTVTSIASEYVKTPAQVLLRWNLQLGNAVVVRSAR
HHCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHEEECEEECCEEEEECCC
PERIASNFDVFDFELAAEHMDALGGLNDGTRVREDPLTYAGT
CHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]