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The map label for this gene is nei [C]

Identifier: 121638347

GI number: 121638347

Start: 2732029

End: 2732835

Strand: Reverse

Name: nei [C]

Synonym: BCG_2484c

Alternate gene names: 121638347

Gene position: 2732835-2732029 (Counterclockwise)

Preceding gene: 121638348

Following gene: 121638345

Centisome position: 62.47

GC content: 66.79

Gene sequence:

>807_bases
GTGCCTGAGGGGCATACGCTGCATCGGCTGGCCCGGCTGCACCAGCGCCGGTTCGCCGGTGCGCCGGTCTCGGTGTCCAG
CCCCCAGGGCCGGTTCGCCGATTCGGCTTCCGCGCTCAACGGTCGGGTGTTGCGGCGGGCCAGTGCCTGGGGCAAACACC
TGTTCCATCACTACGTCGGCGGCCCCGTGGTGCACGTGCATCTCGGGCTCTATGGCACCTTCACCGAATGGGCACGCCCC
ACAGATGGGTGGCTACCCGAACCCGCGGGGCAGGTGCGGATGCGGATGGTCGGGGCAGAATTCGGCACCGACTTGCGCGG
CCCGACGGTGTGTGAGTCGATCGACGACGGCGAGGTTGCGGACGTGGTTGCCAGGCTGGGACCCGACCCGTTGCGTAGCG
ACGCCAACCCGTCGTCGGCATGGTCTCGGATCACCAAGTCTCGCAGGCCCATAGGCGCGCTCTTGATGGACCAAACGGTG
ATCGCCGGTGTTGGCAACGTCTACCGTAATGAGTTGCTTTTTCGGCACCGGATCGACCCACAGCGGCCCGGCCGCGGGAT
CGGTGAGCCGGAATTCGACGCGGCCTGGAACGATCTGGTGTCGTTGATGAAGGTGGGCTTGCGCCGCGGCAAGATCATCG
TGGTGCGCCCCGAACATGACCATGGTCTGCCGTCATATCTGCCCGACCGGCCCCGCACCTATGTGTACCGCCGAGCCGGC
GAGCCGTGTCGGGTGTGTGGGGGAGTGATCCGCACGGCACTGCTCGAGGGCCGCAATGTTTTCTGGTGCCCGGTCTGTCA
GACCTGA

Upstream 100 bases:

>100_bases
GGTCGAAAGCGCAACGCCACCAACGGCGTATCGACATTCTCGCCGAGTACGAACGGACCCACGAAGCGCCGCCGGTGCCC
GGCGCTCCGGCCTGACTCCC

Downstream 100 bases:

>100_bases
CCGCCTGGTGGCTAGCCGTCTAGGAACGGTTTACCCCGAGCGCGGCGTAGACCTCGTTGGACAGGACGACGCTGCGCGGA
TCCGCGTTGGCCTTGGTGGC

Product: putative DNA glycosylase

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase Rv2464c/MT2539; Putative AP lyase Rv2464c/MT2539

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPEGHTLHRLARLHQRRFAGAPVSVSSPQGRFADSASALNGRVLRRASAWGKHLFHHYVGGPVVHVHLGLYGTFTEWARP
TDGWLPEPAGQVRMRMVGAEFGTDLRGPTVCESIDDGEVADVVARLGPDPLRSDANPSSAWSRITKSRRPIGALLMDQTV
IAGVGNVYRNELLFRHRIDPQRPGRGIGEPEFDAAWNDLVSLMKVGLRRGKIIVVRPEHDHGLPSYLPDRPRTYVYRRAG
EPCRVCGGVIRTALLEGRNVFWCPVCQT

Sequences:

>Translated_268_residues
MPEGHTLHRLARLHQRRFAGAPVSVSSPQGRFADSASALNGRVLRRASAWGKHLFHHYVGGPVVHVHLGLYGTFTEWARP
TDGWLPEPAGQVRMRMVGAEFGTDLRGPTVCESIDDGEVADVVARLGPDPLRSDANPSSAWSRITKSRRPIGALLMDQTV
IAGVGNVYRNELLFRHRIDPQRPGRGIGEPEFDAAWNDLVSLMKVGLRRGKIIVVRPEHDHGLPSYLPDRPRTYVYRRAG
EPCRVCGGVIRTALLEGRNVFWCPVCQT
>Mature_267_residues
PEGHTLHRLARLHQRRFAGAPVSVSSPQGRFADSASALNGRVLRRASAWGKHLFHHYVGGPVVHVHLGLYGTFTEWARPT
DGWLPEPAGQVRMRMVGAEFGTDLRGPTVCESIDDGEVADVVARLGPDPLRSDANPSSAWSRITKSRRPIGALLMDQTVI
AGVGNVYRNELLFRHRIDPQRPGRGIGEPEFDAAWNDLVSLMKVGLRRGKIIVVRPEHDHGLPSYLPDRPRTYVYRRAGE
PCRVCGGVIRTALLEGRNVFWCPVCQT

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1786932, Length=276, Percent_Identity=26.0869565217391, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790066, Length=147, Percent_Identity=31.2925170068027, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2464_MYCTU (P64158)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   G70865
- RefSeq:   NP_216980.1
- RefSeq:   NP_337025.1
- ProteinModelPortal:   P64158
- SMR:   P64158
- EnsemblBacteria:   EBMYCT00000002822
- EnsemblBacteria:   EBMYCT00000069873
- GeneID:   888500
- GeneID:   925782
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2539
- KEGG:   mtu:Rv2464c
- TIGR:   MT2539
- TubercuList:   Rv2464c
- GeneTree:   EBGT00050000016359
- HOGENOM:   HBG690070
- OMA:   EVYVYRR
- ProtClustDB:   CLSK791864
- BRENDA:   4.2.99.18
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =4.2.99.18

Molecular weight: Translated: 29714; Mature: 29583

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 52-52 ACT_SITE 258-258 BINDING 125-125 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGHTLHRLARLHQRRFAGAPVSVSSPQGRFADSASALNGRVLRRASAWGKHLFHHYVG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GPVVHVHLGLYGTFTEWARPTDGWLPEPAGQVRMRMVGAEFGTDLRGPTVCESIDDGEVA
CCEEEEEECEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHH
DVVARLGPDPLRSDANPSSAWSRITKSRRPIGALLMDQTVIAGVGNVYRNELLFRHRIDP
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QRPGRGIGEPEFDAAWNDLVSLMKVGLRRGKIIVVRPEHDHGLPSYLPDRPRTYVYRRAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCC
EPCRVCGGVIRTALLEGRNVFWCPVCQT
CHHHHHHHHHHHHHHCCCCEEEECCCCC
>Mature Secondary Structure 
PEGHTLHRLARLHQRRFAGAPVSVSSPQGRFADSASALNGRVLRRASAWGKHLFHHYVG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
GPVVHVHLGLYGTFTEWARPTDGWLPEPAGQVRMRMVGAEFGTDLRGPTVCESIDDGEVA
CCEEEEEECEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHH
DVVARLGPDPLRSDANPSSAWSRITKSRRPIGALLMDQTVIAGVGNVYRNELLFRHRIDP
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QRPGRGIGEPEFDAAWNDLVSLMKVGLRRGKIIVVRPEHDHGLPSYLPDRPRTYVYRRAG
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCC
EPCRVCGGVIRTALLEGRNVFWCPVCQT
CHHHHHHHHHHHHHHCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036