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The map label for this gene is tig

Identifier: 121638345

GI number: 121638345

Start: 2729061

End: 2730461

Strand: Reverse

Name: tig

Synonym: BCG_2482c

Alternate gene names: 121638345

Gene position: 2730461-2729061 (Counterclockwise)

Preceding gene: 121638347

Following gene: 121638344

Centisome position: 62.42

GC content: 65.02

Gene sequence:

>1401_bases
GTGAAGAGCACCGTCGAGCAGTTGAGCCCCACCCGGGTTCGTATCAACGTGGAGGTGCCATTCGCCGAGCTTGAGCCGGA
TTTCCAGCGGGCCTACAAAGAGCTGGCCAAACAGGTGCGGCTGCCCGGCTTCCGGCCCGGGAAGGCGCCGGCCAAACTAC
TCGAAGCCCGCATCGGCCGGGAGGCCATGCTGGATCAAATCGTCAACGATGCGCTGCCCAGCCGGTACGGACAGGCGGTG
GCCGAGTCGGATGTCCAACCGCTCGGCCGGCCCAACATCGAGGTGACCAAGAAGGAGTACGGCCAGGACCTGCAATTCAC
CGCCGAGGTCGACATCCGCCCGAAGATCAGTCTCCCGGACCTGAGCGCGCTGACGGTCTCGGTGGATCCGATCGAAATCG
GTGAGGACGACGTCGACGCCGAACTGCAGTCGTTACGTACCCGGTTCGGCACCCTGACCGCGGTGGACCGGCCGGTGGCC
GTCGGCGACGTCGTCTCGATCGACTTGTCTGCCACGGTCGACGGAGAGGACATACCGAACGCAGCCGCTGAGGGACTCTC
CCACGAGGTCGGCTCCGGCCGGCTCATCGCAGGTCTCGACGACGCGGTTGTTGGTCTGTCCGCCGACGAGTCCCGGGTCT
TCACCGCCAAGCTGGCAGCCGGCGAGCACGCCGGGCAGGAAGCTCAGGTTACCGTCACGGTCAGGTCGGTTAAGGAGCGC
GAACTACCAGAGCCCGACGACGAATTCGCGCAGTTAGCCAGCGAGTTCGACAGCATCGACGAATTGCGGGCCAGCCTCAG
CGACCAGGTGCGCCAGGCCAAGCGCGCCCAGCAGGCCGAGCAGATTCGAAACGCCACCATCGATGCGCTACTCGAACAGG
TCGACGTGCCGTTGCCGGAGTCGTATGTGCAGGCCCAATTCGACAGCGTGCTGCACAGCGCGCTCAGCGGTCTTAATCAC
GACGAAGCCCGGTTCAATGAGTTGCTCGTCGAGCAAGGCTCGTCACGCGCGGCGTTCGATGCCGAGGCGCGCACCGCCTC
AGAAAAGGACGTCAAGAGGCAGCTGTTGCTAGACGCCCTGGCCGATGAGCTGCAGGTCCAAGTTGGCCAGGATGATCTGA
CCGAACGACTGGTGACGACGTCTCGGCAATACGGCATCGAGCCGCAGCAGCTGTTCGGCTACCTCCAAGAGCGCAACCAG
CTGCCGACCATGTTCGCTGACGTGCGGCGCGAGCTGGCGATCAGGGCCGCAGTGGAGGCGGCGACGGTCACCGACAGTGA
CGGAAACACGATCGATACCAGTGAGTTCTTCGGCAAGCGTGTGTCGGCCGGTGAGGCTGAGGAGGCCGAACCGGCAGACG
AGGGTGCCGCGCGGGCGGCGTCCGACGAAGCGACAACGTGA

Upstream 100 bases:

>100_bases
AGCTTGGTAGCGCATCCGCTTTGGGAGCGGAAGGCCGCAGGTTCAAATCCTGTCACCCCGACCAGCCGACCGCCGTTATC
GACTACCAAGGAGCACACCC

Downstream 100 bases:

>100_bases
CCGTATGACGCTGTAAGCGAACGCGCCGGTTTCAGGGGGTGCGTGACCGCGAAACAGCCAGCCTGGTTGGTTAGTGTCGG
AGCATAGAAGAACTCGAGGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGREAMLDQIVNDALPSRYGQAV
AESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISLPDLSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVA
VGDVVSIDLSATVDGEDIPNAAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPESYVQAQFDSVLHSALSGLNH
DEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDALADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQ
LPTMFADVRRELAIRAAVEAATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT

Sequences:

>Translated_466_residues
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGREAMLDQIVNDALPSRYGQAV
AESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISLPDLSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVA
VGDVVSIDLSATVDGEDIPNAAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPESYVQAQFDSVLHSALSGLNH
DEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDALADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQ
LPTMFADVRRELAIRAAVEAATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT
>Mature_466_residues
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGREAMLDQIVNDALPSRYGQAV
AESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISLPDLSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVA
VGDVVSIDLSATVDGEDIPNAAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPESYVQAQFDSVLHSALSGLNH
DEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDALADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQ
LPTMFADVRRELAIRAAVEAATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=333, Percent_Identity=25.8258258258258, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_MYCBO (Q7TYJ1)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856136.1
- ProteinModelPortal:   Q7TYJ1
- SMR:   Q7TYJ1
- EnsemblBacteria:   EBMYCT00000016517
- GeneID:   1094228
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2489c
- GeneTree:   EBGT00050000015814
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   MBOV233413:MB2489C-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 50633; Mature: 50633

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGR
CCCCHHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCH
EAMLDQIVNDALPSRYGQAVAESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISLPD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHCCCCCEEEEEECCCCCCCCCC
LSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVAVGDVVSIDLSATVDGEDIPN
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCEECCEEEEEEEEEECCCCCCH
AAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
HHHHHHHHCCCCCCEEECCCCHHEECCCCCCEEEEEEECCCCCCCCCCEEEEEEEHHHHH
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SYVQAQFDSVLHSALSGLNHDEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
ADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQLPTMFADVRRELAIRAAVEA
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT
HEEECCCCCEECCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSTVEQLSPTRVRINVEVPFAELEPDFQRAYKELAKQVRLPGFRPGKAPAKLLEARIGR
CCCCHHHCCCCEEEEEEECCHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCH
EAMLDQIVNDALPSRYGQAVAESDVQPLGRPNIEVTKKEYGQDLQFTAEVDIRPKISLPD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECHHHCCCCCEEEEEECCCCCCCCCC
LSALTVSVDPIEIGEDDVDAELQSLRTRFGTLTAVDRPVAVGDVVSIDLSATVDGEDIPN
CCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCEECCEEEEEEEEEECCCCCCH
AAAEGLSHEVGSGRLIAGLDDAVVGLSADESRVFTAKLAAGEHAGQEAQVTVTVRSVKER
HHHHHHHHCCCCCCEEECCCCHHEECCCCCCEEEEEEECCCCCCCCCCEEEEEEEHHHHH
ELPEPDDEFAQLASEFDSIDELRASLSDQVRQAKRAQQAEQIRNATIDALLEQVDVPLPE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SYVQAQFDSVLHSALSGLNHDEARFNELLVEQGSSRAAFDAEARTASEKDVKRQLLLDAL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHH
ADELQVQVGQDDLTERLVTTSRQYGIEPQQLFGYLQERNQLPTMFADVRRELAIRAAVEA
HHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ATVTDSDGNTIDTSEFFGKRVSAGEAEEAEPADEGAARAASDEATT
HEEECCCCCEECCHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972