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The map label for this gene is folC [H]

Identifier: 121638330

GI number: 121638330

Start: 2711305

End: 2712768

Strand: Reverse

Name: folC [H]

Synonym: BCG_2467c

Alternate gene names: 121638330

Gene position: 2712768-2711305 (Counterclockwise)

Preceding gene: 121638331

Following gene: 121638329

Centisome position: 62.01

GC content: 67.76

Gene sequence:

>1464_bases
ATGAATTCGACGAATTCCGGCCCGCCTGACTCGGGATCGGCCACCGGCGTCGTGCCCACTCCGGACGAGATCGCGTCCCT
GCTGCAGGTTGAGCATCTACTCGACCAACGCTGGCCGGAGACCCGCATCGATCCGAGCCTGACCCGGATCAGCGCGTTGA
TGGACCTGCTGGGCTCGCCCCAACGCAGCTATCCGTCGATCCATATCGCGGGCACCAACGGCAAGACCTCGGTGGCGCGC
ATGGTCGACGCGCTGGTCACCGCGCTGCACCGGCGCACCGGCCGAACCACCAGCCCACACCTGCAGTCACCGGTGGAACG
CATTTCGATCGACGGCAAGCCGATCAGCCCGGCGCAGTATGTGGCGACCTACCGGGAGATCGAGCCGTTGGTGGCGCTGA
TCGACCAGCAGTCGCAGGCTTCTGCGGGTAAGGGTGGCCCGGCGATGAGCAAGTTCGAGGTGCTCACCGCGATGGCGTTC
GCGGCCTTTGCGGACGCGCCCGTCGACGTGGCAGTGGTCGAGGTGGGCATGGGCGGACGTTGGGACGCCACCAACGTGAT
CAACGCACCGGTCGCCGTCATCACCCCGATCAGCATTGATCACGTCGACTATCTCGGTGCCGATATCGCCGGGATCGCCG
GGGAGAAGGCGGGCATCATCACTCGGGCCCCCGACGGTTCGCCGGACACCGTCGCGGTCATCGGGCGTCAGGTCCCGAAG
GTCATGGAGGTGCTGCTGGCCGAATCGGTGCGCGCCGACGCGTCGGTGGCCCGGGAGGATTCCGAATTCGCGGTGCTACG
GCGACAGATCGCGGTCGGCGGTCAGGTACTGCAACTGCAGGGCCTCGGCGGGGTTTACTCCGACATCTACTTGCCGCTGC
ACGGTGAACACCAGGCGCACAACGCGGTGCTCGCCCTCGCTTCCGTCGAGGCCTTTTTCGGTGCCGGTGCGCAGCGTCAG
CTCGACGGCGACGCCGTCCGGGCCGGCTTTGCCGCCGTCACCAGTCCCGGCCGGTTGGAGCGCATGCGCAGCGCACCCAC
GGTGTTCATCGACGCCGCGCACAATCCGGCCGGGGCGAGTGCTCTGGCACAAACGCTGGCGCATGAGTTCGACTTCCGAT
TTCTGGTCGGGGTGCTCAGCGTGCTGGGCGACAAGGACGTGGACGGCATCCTGGCCGCACTGGAGCCGGTGTTCGATTCC
GTCGTCGTGACCCACAACGGGTCGCCGCGGGCGCTGGATGTCGAGGCCCTGGCGCTGGCGGCCGGCGAGCGGTTCGGACC
CGACCGGGTGCGCACCGCCGAGAACCTGCGCGATGCTATCGACGTTGCCACCTCACTGGTCGACGACGCCGCCGCCGACC
CGGATGTGGCCGGGGACGCATTCTCGAGAACCGGGATCGTCATCACCGGCTCGGTTGTCACCGCAGGGGCGGCTCGGACC
TTGTTCGGTCGTGATCCGCAATGA

Upstream 100 bases:

>100_bases
TGGCCAAAGCGCCCGACGCCGTCATTGCCAAGATCCGGGACCGCCAGCGCGTGGCGCAGCAGGAAACCGAGCGCATCACC
ACCCGGTTGGCTGCGCTGCA

Downstream 100 bases:

>100_bases
CCGACCGGTCGCGTGAGCCGGCTGACCCGTGGAAGGGATTCAGCGCGGTGATGGCGGCGACGCTGATCCTCGAGGCGATC
GTGGTGCTGCTGGCAATACC

Product: putative folylpolyglutamate synthase protein folC

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MNSTNSGPPDSGSATGVVPTPDEIASLLQVEHLLDQRWPETRIDPSLTRISALMDLLGSPQRSYPSIHIAGTNGKTSVAR
MVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAF
AAFADAPVDVAVVEVGMGGRWDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPDGSPDTVAVIGRQVPK
VMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAGAQRQ
LDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDS
VVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGDAFSRTGIVITGSVVTAGAART
LFGRDPQ

Sequences:

>Translated_487_residues
MNSTNSGPPDSGSATGVVPTPDEIASLLQVEHLLDQRWPETRIDPSLTRISALMDLLGSPQRSYPSIHIAGTNGKTSVAR
MVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAF
AAFADAPVDVAVVEVGMGGRWDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPDGSPDTVAVIGRQVPK
VMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAGAQRQ
LDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDS
VVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGDAFSRTGIVITGSVVTAGAART
LFGRDPQ
>Mature_487_residues
MNSTNSGPPDSGSATGVVPTPDEIASLLQVEHLLDQRWPETRIDPSLTRISALMDLLGSPQRSYPSIHIAGTNGKTSVAR
MVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQYVATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAF
AAFADAPVDVAVVEVGMGGRWDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPDGSPDTVAVIGRQVPK
VMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAHNAVLALASVEAFFGAGAQRQ
LDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGASALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDS
VVVTHNGSPRALDVEALALAAGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGDAFSRTGIVITGSVVTAGAART
LFGRDPQ

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=376, Percent_Identity=29.2553191489362, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI66932990, Length=376, Percent_Identity=29.2553191489362, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI1788654, Length=388, Percent_Identity=29.8969072164948, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=454, Percent_Identity=24.0088105726872, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71984923, Length=454, Percent_Identity=24.0088105726872, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17553150, Length=460, Percent_Identity=24.3478260869565, Blast_Score=101, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=374, Percent_Identity=32.8877005347594, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=189, Percent_Identity=33.8624338624339, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322718, Length=193, Percent_Identity=27.4611398963731, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641571, Length=188, Percent_Identity=33.5106382978723, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24581568, Length=160, Percent_Identity=33.125, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 50780; Mature: 50780

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTNSGPPDSGSATGVVPTPDEIASLLQVEHLLDQRWPETRIDPSLTRISALMDLLGSP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
QRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQY
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHEECCCCCCCHHHH
VATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAFAAFADAPVDVAVVEVGMGGR
HHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
WDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPDGSPDTVAVIGRQVPK
CCCCCCCCCCEEEEECCCCCHHHHHCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHH
VMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHEEEECCCCCCHH
NAVLALASVEAFFGAGAQRQLDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAS
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCCCHH
ALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDSVVVTHNGSPRALDVEALALA
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHHH
AGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGDAFSRTGIVITGSVVTAGAART
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCHHH
LFGRDPQ
CCCCCCC
>Mature Secondary Structure
MNSTNSGPPDSGSATGVVPTPDEIASLLQVEHLLDQRWPETRIDPSLTRISALMDLLGSP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
QRSYPSIHIAGTNGKTSVARMVDALVTALHRRTGRTTSPHLQSPVERISIDGKPISPAQY
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCHHHHEECCCCCCCHHHH
VATYREIEPLVALIDQQSQASAGKGGPAMSKFEVLTAMAFAAFADAPVDVAVVEVGMGGR
HHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
WDATNVINAPVAVITPISIDHVDYLGADIAGIAGEKAGIITRAPDGSPDTVAVIGRQVPK
CCCCCCCCCCEEEEECCCCCHHHHHCCCHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHH
VMEVLLAESVRADASVAREDSEFAVLRRQIAVGGQVLQLQGLGGVYSDIYLPLHGEHQAH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHEEEECCCCCCHH
NAVLALASVEAFFGAGAQRQLDGDAVRAGFAAVTSPGRLERMRSAPTVFIDAAHNPAGAS
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCEEEEECCCCCCCHH
ALAQTLAHEFDFRFLVGVLSVLGDKDVDGILAALEPVFDSVVVTHNGSPRALDVEALALA
HHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHHHH
AGERFGPDRVRTAENLRDAIDVATSLVDDAAADPDVAGDAFSRTGIVITGSVVTAGAART
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCHHH
LFGRDPQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]