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The map label for this gene is proA

Identifier: 121638309

GI number: 121638309

Start: 2689400

End: 2690647

Strand: Reverse

Name: proA

Synonym: BCG_2444c

Alternate gene names: 121638309

Gene position: 2690647-2689400 (Counterclockwise)

Preceding gene: 121638312

Following gene: 121638308

Centisome position: 61.51

GC content: 67.79

Gene sequence:

>1248_bases
ATGACCGTGCCAGCACCGTCGCAGCTCGACTTGCGTCAAGAGGTGCACGACGCCGCACGCCGCGCCCGGGTGGCCGCCCG
CCGGCTGGCATCGCTGCCGACGACTGTCAAAGACCGCGCGCTGCACGCGGCTGCCGACGAGCTACTGGCTCACCGCGACC
AGATCCTGGCGGCCAACGCCGAAGACCTGAACGCGGCGCGCGAGGCGGACACCCCGGCCGCCATGCTGGACCGGTTGTCC
TTGAACCCGCAACGAGTCGACGGTATCGCCGCCGGGTTGCGGCAAGTCGCGGGACTGCGCGATCCGGTCGGTGAAGTGCT
GCGTGGCTATACCCTGCCCAACGGGCTGCAGCTGCGCCAGCAGCGCGTCCCCCTGGGCGTGGTCGGCATGATCTACGAGG
GCCGCCCCAATGTCACCGTGGATGCCTTCGGGCTGACACTCAAGTCGGGTAACGCTGCATTGCTGCGCGGCAGCTCGTCG
GCCGCAAAGTCCAACGAGGCCCTGGTGGCGGTGTTACGCACCGCGCTGGTCGGCCTGGAGCTGCCGGCCGACGCGGTCCA
GCTGCTGTCGGCTGCCGACCGCGCCACCGTCACTCACCTGATTCAGGCCCGCGGCCTGGTCGATGTGGTGATTCCACGCG
GGGGAGCGGGCCTGATCGAGGCGGTCGTACGCGATGCCCAGGTGCCCACCATCGAGACCGGCGTCGGGAACTGCCATGTC
TACGTGCACCAAGCGGCCGACCTGGACGTGGCCGAGCGTATCTTGCTGAACTCCAAGACGCGGCGGCCCAGCGTCTGCAA
CGCCGCCGAGACGCTGCTGGTCGACGCAGCGATCGCCGAAACGGCGTTGCCTCGATTGCTGGCCGCCCTGCAGCACGCCG
GTGTCACCGTACATCTCGACCCGGACGAGGCCGACCTGCGCCGCGAATACCTGTCGCTGGACATCGCGGTGGCGGTGGTC
GACGGTGTCGACGCTGCCATCGCCCATATCAACGAATACGGCACCGGGCACACAGAAGCGATTGTGACCACCAATCTTGA
TGCGGCCCAACGCTTTACCGAACAGATCGATGCGGCCGCGGTGATGGTGAACGCATCAACGGCGTTCACCGACGGCGAGC
AATTCGGCTTCGGCGCCGAGATCGGCATCTCCACCCAGAAACTGCATGCCCGCGGACCGATGGGACTACCGGAATTGACG
TCGACCAAGTGGATCGCATGGGGAGCCGGCCACACCCGTCCGGCCTGA

Upstream 100 bases:

>100_bases
GTACGCCTGATCGCATAGCAACCACCCAGCCGGATCGCTGCGCGGCCCGTCCGGAGCGGGTCGTCTTACCTGGCCAGCGC
AAGCCCGTTACCCTGGGGCA

Downstream 100 bases:

>100_bases
CGCCGTTGGCTAGAGGAGACCCGATCGTGACCGTGCCCGCCCGGCCCACGCCGCTGTTCGCCGACATCGCCGACGTCTCG
CGGCGGCTGGCCGAGACCGG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTVPAPSQLDLRQEVHDAARRARVAARRLASLPTTVKDRALHAAADELLAHRDQILAANAEDLNAAREADTPAAMLDRLS
LNPQRVDGIAAGLRQVAGLRDPVGEVLRGYTLPNGLQLRQQRVPLGVVGMIYEGRPNVTVDAFGLTLKSGNAALLRGSSS
AAKSNEALVAVLRTALVGLELPADAVQLLSAADRATVTHLIQARGLVDVVIPRGGAGLIEAVVRDAQVPTIETGVGNCHV
YVHQAADLDVAERILLNSKTRRPSVCNAAETLLVDAAIAETALPRLLAALQHAGVTVHLDPDEADLRREYLSLDIAVAVV
DGVDAAIAHINEYGTGHTEAIVTTNLDAAQRFTEQIDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARGPMGLPELT
STKWIAWGAGHTRPA

Sequences:

>Translated_415_residues
MTVPAPSQLDLRQEVHDAARRARVAARRLASLPTTVKDRALHAAADELLAHRDQILAANAEDLNAAREADTPAAMLDRLS
LNPQRVDGIAAGLRQVAGLRDPVGEVLRGYTLPNGLQLRQQRVPLGVVGMIYEGRPNVTVDAFGLTLKSGNAALLRGSSS
AAKSNEALVAVLRTALVGLELPADAVQLLSAADRATVTHLIQARGLVDVVIPRGGAGLIEAVVRDAQVPTIETGVGNCHV
YVHQAADLDVAERILLNSKTRRPSVCNAAETLLVDAAIAETALPRLLAALQHAGVTVHLDPDEADLRREYLSLDIAVAVV
DGVDAAIAHINEYGTGHTEAIVTTNLDAAQRFTEQIDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARGPMGLPELT
STKWIAWGAGHTRPA
>Mature_414_residues
TVPAPSQLDLRQEVHDAARRARVAARRLASLPTTVKDRALHAAADELLAHRDQILAANAEDLNAAREADTPAAMLDRLSL
NPQRVDGIAAGLRQVAGLRDPVGEVLRGYTLPNGLQLRQQRVPLGVVGMIYEGRPNVTVDAFGLTLKSGNAALLRGSSSA
AKSNEALVAVLRTALVGLELPADAVQLLSAADRATVTHLIQARGLVDVVIPRGGAGLIEAVVRDAQVPTIETGVGNCHVY
VHQAADLDVAERILLNSKTRRPSVCNAAETLLVDAAIAETALPRLLAALQHAGVTVHLDPDEADLRREYLSLDIAVAVVD
GVDAAIAHINEYGTGHTEAIVTTNLDAAQRFTEQIDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARGPMGLPELTS
TKWIAWGAGHTRPA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=411, Percent_Identity=38.4428223844282, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI21361368, Length=411, Percent_Identity=38.4428223844282, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1786438, Length=405, Percent_Identity=47.6543209876543, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17569967, Length=413, Percent_Identity=37.2881355932203, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17569969, Length=413, Percent_Identity=37.2881355932203, Blast_Score=255, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324899, Length=427, Percent_Identity=33.9578454332553, Blast_Score=236, Evalue=3e-63,
Organism=Drosophila melanogaster, GI21357643, Length=409, Percent_Identity=37.4083129584352, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_MYCBO (P65789)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856100.1
- ProteinModelPortal:   P65789
- SMR:   P65789
- EnsemblBacteria:   EBMYCT00000015775
- GeneID:   1094264
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2451c
- GeneTree:   EBGT00050000015663
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   MBOV233413:MB2451C-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 43745; Mature: 43614

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPAPSQLDLRQEVHDAARRARVAARRLASLPTTVKDRALHAAADELLAHRDQILAANA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCC
EDLNAAREADTPAAMLDRLSLNPQRVDGIAAGLRQVAGLRDPVGEVLRGYTLPNGLQLRQ
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHH
QRVPLGVVGMIYEGRPNVTVDAFGLTLKSGNAALLRGSSSAAKSNEALVAVLRTALVGLE
HCCCEEEEEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
LPADAVQLLSAADRATVTHLIQARGLVDVVIPRGGAGLIEAVVRDAQVPTIETGVGNCHV
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEECCCCCEEE
YVHQAADLDVAERILLNSKTRRPSVCNAAETLLVDAAIAETALPRLLAALQHAGVTVHLD
EEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PDEADLRREYLSLDIAVAVVDGVDAAIAHINEYGTGHTEAIVTTNLDAAQRFTEQIDAAA
CCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEE
VMVNASTAFTDGEQFGFGAEIGISTQKLHARGPMGLPELTSTKWIAWGAGHTRPA
EEEECCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
TVPAPSQLDLRQEVHDAARRARVAARRLASLPTTVKDRALHAAADELLAHRDQILAANA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCC
EDLNAAREADTPAAMLDRLSLNPQRVDGIAAGLRQVAGLRDPVGEVLRGYTLPNGLQLRQ
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHH
QRVPLGVVGMIYEGRPNVTVDAFGLTLKSGNAALLRGSSSAAKSNEALVAVLRTALVGLE
HCCCEEEEEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
LPADAVQLLSAADRATVTHLIQARGLVDVVIPRGGAGLIEAVVRDAQVPTIETGVGNCHV
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCEEECCCCCEEE
YVHQAADLDVAERILLNSKTRRPSVCNAAETLLVDAAIAETALPRLLAALQHAGVTVHLD
EEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PDEADLRREYLSLDIAVAVVDGVDAAIAHINEYGTGHTEAIVTTNLDAAQRFTEQIDAAA
CCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHEE
VMVNASTAFTDGEQFGFGAEIGISTQKLHARGPMGLPELTSTKWIAWGAGHTRPA
EEEECCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972