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The map label for this gene is mbtI

Identifier: 121638265

GI number: 121638265

Start: 2643675

End: 2645027

Strand: Reverse

Name: mbtI

Synonym: BCG_2400c

Alternate gene names: 121638265

Gene position: 2645027-2643675 (Counterclockwise)

Preceding gene: 121638267

Following gene: 121638262

Centisome position: 60.46

GC content: 64.15

Gene sequence:

>1353_bases
GTGTCCGAGCTCAGCGTCGCGACAGGCGCCGTCAGCACCGCGTCGTCGTCCATCCCGATGCCCGCCGGTGTCAACCCCGC
CGACCTGGCAGCGGAGCTGGCGGCGGTGGTTACCGAGTCCGTCGACGAGGATTACCTGCTCTACGAGTGCGACGGCCAAT
GGGTCCTGGCCGCCGGTGTGCAGGCGATGGTGGAGCTAGACAGCGACGAACTGCGCGTCATCCGTGATGGCGTTACGCGG
CGACAGCAATGGTCGGGTCGCCCGGGAGCGGCCCTGGGCGAAGCCGTCGATCGGCTGTTGCTGGAAACCGATCAAGCTTT
TGGCTGGGTCGCCTTCGAATTCGGCGTGCACCGCTATGGGTTGCAGCAGCGGCTGGCGCCGCACACCCCACTGGCCCGGG
TGTTTTCGCCCCGAACCCGGATCATGGTGAGCGAAAAGGAGATTCGCCTGTTCGATGCTGGGATTCGCCACCGCGAGGCC
ATCGACCGATTACTCGCCACCGGGGTGCGAGAGGTGCCGCAGTCCCGCTCCGTCGACGTCTCCGACGATCCATCCGGCTT
CCGCCGTCGGGTGGCGGTAGCCGTCGATGAAATCGCTGCCGGCCGCTACCACAAGGTGATTCTGTCCCGTTGTGTCGAAG
TGCCTTTCGCGATCGACTTTCCGTTGACCTACCGGCTGGGGCGTCGGCACAACACCCCGGTGAGGTCGTTTTTGTTGCAG
TTGGGCGGAATCCGTGCTCTGGGTTACAGCCCCGAACTCGTCACGGCGGTGCGCGCCGACGGAGTGGTGATCACCGAGCC
GTTGGCCGGTACCCGCGCCTTGGGCCGTGGTCCCGCCATTGACCGACTGGCTCGTGATGACCTGGAATCAAACTCCAAAG
AAATTGTCGAGCACGCCATTTCAGTGCGCTCTTCGCTTGAGGAGATTACCGACATCGCCGAACCAGGGAGTGCTGCGGTC
ATCGATTTCATGACGGTGCGCGAGCGCGGCAGTGTGCAGCACCTCGGCTCCACCATCAGAGCACGGTTGGATCCATCGAG
CGACCGGATGGCCGCCCTGGAAGCCCTTTTTCCTGCTGTCACTGCATCCGGAATCCCGAAAGCAGCTGGCGTTGAGGCCA
TCTTTCGCCTCGATGAGTGCCCACGTGGGCTGTATTCCGGTGCGGTGGTGATGCTTTCGGCGGATGGCGGGCTAGACGCC
GCGCTGACGCTGCGGGCGGCATACCAGGTCGGCGGGCGGACTTGGCTGCGGGCCGGCGCCGGCATCATCGAAGAATCGGA
GCCAGAGCGCGAATTCGAGGAGACTTGCGAAAAGCTATCCACATTGACGCCTTATCTGGTTGCACGCCAGTAA

Upstream 100 bases:

>100_bases
TTGCTCCCGACGAGCATTTTCTTCCAGTCGAGCACGTTGGACATGCGACGACCCCGGGGGGCTGCGACCCATTAAGTAGG
TTAGGCTACATTTACTAGCT

Downstream 100 bases:

>100_bases
CGTTCAGAGGTCTCCACGTGCTGTTGTACTGCGGATGAACTGGGCAACATAGGTCATGGCTTTGGCCGCTTCGGGCGTCA
TCCGCGGCAGGGCCTGGAAC

Product: salicylate synthase MbtI

Products: NA

Alternate protein names: Mycobactin synthase protein I; Salicylate synthase mbtI

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTR
RQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREA
IDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQ
LGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAV
IDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDA
ALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ

Sequences:

>Translated_450_residues
MSELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTR
RQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREA
IDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQ
LGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAV
IDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDA
ALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ
>Mature_449_residues
SELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRR
QQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAI
DRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQL
GGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVI
DFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAA
LTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ

Specific function: Mediates the production of salicylate from chorismate via an isochorismate intermediate. Presents both isochorismate synthase and isochorismate-pyruvate lyase activities. Salycilate is the starter unit in the production of the virulence-conferring salicyl

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family. Salicylate synthase subfamily

Homologues:

Organism=Escherichia coli, GI1787518, Length=251, Percent_Identity=30.6772908366534, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788114, Length=246, Percent_Identity=28.8617886178862, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082077, Length=267, Percent_Identity=28.8389513108614, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1786809, Length=288, Percent_Identity=28.125, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=255, Percent_Identity=31.3725490196078, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): MBTI_MYCBO (Q7TYQ1)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856056.1
- HSSP:   Q9X9I8
- ProteinModelPortal:   Q7TYQ1
- SMR:   Q7TYQ1
- EnsemblBacteria:   EBMYCT00000018001
- GeneID:   1090601
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2407c
- GeneTree:   EBGT00050000015088
- HOGENOM:   HBG326929
- OMA:   TPEREFE
- ProtClustDB:   PRK07912
- BioCyc:   MBOV233413:MB2407C-MONOMER
- BRENDA:   5.4.4.2
- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR015890
- InterPro:   IPR019996
- Gene3D:   G3DSA:3.60.120.10
- PANTHER:   PTHR11236
- PRINTS:   PR00095
- TIGRFAMs:   TIGR03494

Pfam domain/function: PF00425 Chorismate_bind; SSF56322 TRPE_1_chor_bd

EC number: =5.4.4.2

Molecular weight: Translated: 48755; Mature: 48624

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: BINDING 271-271 BINDING 334-334 BINDING 385-385 BINDING 405-405 BINDING 438-438

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGV
CCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHHH
QAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYG
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
LQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDV
HHHHCCCCCCHHHHHCCCCEEEEECCCEEHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
SDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQ
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHHHH
LGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAI
HCCEEEECCCCHHHHHHCCCCEEEECCCCCCHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
SVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAV
HHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGA
HHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCC
GIIEESEPEREFEETCEKLSTLTPYLVARQ
CCCCCCCCHHHHHHHHHHHHHCCCHHEECC
>Mature Secondary Structure 
SELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGV
CCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHHH
QAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYG
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
LQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDV
HHHHCCCCCCHHHHHCCCCEEEEECCCEEHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
SDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQ
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCHHHHHCCCCCCHHHHHHHH
LGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAI
HCCEEEECCCCHHHHHHCCCCEEEECCCCCCHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
SVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAV
HHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGA
HHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHCC
GIIEESEPEREFEETCEKLSTLTPYLVARQ
CCCCCCCCHHHHHHHHHHHHHCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972