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The map label for this gene is stp [H]

Identifier: 121638220

GI number: 121638220

Start: 2583846

End: 2585417

Strand: Reverse

Name: stp [H]

Synonym: BCG_2355c

Alternate gene names: 121638220

Gene position: 2585417-2583846 (Counterclockwise)

Preceding gene: 121638224

Following gene: 121638216

Centisome position: 59.1

GC content: 65.33

Gene sequence:

>1572_bases
ATGAACCGCACGCAGCTCCTCACGCTCATCGCCACTGGCCTGGGCCTGTTCATGATCTTTCTCGACGCACTGATCGTGAA
CGTGGCGCTGCCCGACATCCAGCGCAGTTTCGCGGTGGGGGAGGACGGCCTGCAGTGGGTGGTGGCGTCCTACAGCCTCG
GAATGGCGGTCTTCATCATGTCGGCGGCGACGCTTGCCGACCTCTACGGTCGGCGCCGCTGGTACTTGATAGGTGTTTCG
TTGTTCACGCTGGGATCTATCGCCTGCGGGTTGGCGCCCTCAATCGCGGTGCTCACCACCGCGCGGGGCGCGCAGGGCCT
AGGTGCGGCGGCCGTCAGCGTAACCTCGCTCGCCCTGGTCAGCGCGGCCTTTCCGGAGGCGAAGGAGAAGGCGCGCGCCA
TCGGGATCTGGACCGCCATCGCGAGCATCGGTACGACGACGGGCCCGACACTGGGCGGTCTGCTGGTGGACCAGTGGGGG
TGGCGCAGCATCTTCTATGTCAACCTGCCCATGGGCGCACTCGTCCTCTTCCTGACTTTGTGCTACGTCGAGGAGTCGTG
TAACGAGCGGGCACGCCGCTTCGATCTGTCCGGGCAGCTGCTGTTCATCGTTGCCGTGGGTGCCCTGGTGTATGCGGTCA
TCGAAGGCCCCCAGATCGGCTGGACGTCTGTCCAGACCATCGTGATGCTCTGGACAGCCGCCGTCGGCTGCGCCCTGTTC
GTCTGGCTTGAGCGCAGATCATCGAATCCGATGATGGATCTGACCCTGTTCCGCGACACCTCATACGCCCTGGCCATCGC
GACCATCTGCACGGTGTTCTTCGCCGTCTATGGGATGCTGCTGCTCACCACCCAGTTCCTGCAGAACGTGCGCGGCTATA
CCCCGAGCGTGACCGGCCTGATGATCCTTCCGTTCAGTGCGGCCGTGGCCATCGTGTCGCCACTTGTTGGGCACCTGGTT
GGCCGGATCGGAGCACGCGTTCCCATCCTGGCGGGGCTGTGCATGTTGATGCTGGGTCTGCTCATGCTCATCTTCAGCGA
GCACCGGAGCTCGGCCCTGGTCCTGGTCGGGCTGGGCTTGTGCGGGAGCGGCGTCGCGTTGTGCCTGACGCCGATCACGA
CCGTCGCCATGACCGCGGTCCCCGCCGAGCGTGCGGGCATGGCGTCCGGCATCATGAGTGCTCAGCGGGCGATCGGCTCG
ACGATCGGGTTCGCGGTTTTGGGCTCCGTCCTTGCCGCCTGGCTTTCGGCCACACTCGAGCCCCATCTGGAGCGCGCGGT
GCCCGACCCGGTCCAGCGCCACGTCCTTGCCGAGATCATTATCGACAGTGCCAATCCGCGAGCGCATGTGGGCGGAATCG
TCCCCCGGCGGCACATTGAGCATCGCGACCCCGTCGCGATTGCGGAGGAGGATTTCATCGAAGGCATTCGGGTGGCGTTG
CTCGTCGCCACTGCAACACTGGCTGTCGTGTTTCTCGCCGGCTGGCGATGGTTTCCGCGTGACGTGCAAACCGCGGGCAG
CGACGCTAAGCGAGAGGCTGCCTACAGCGATGACCGTCGAGTGCGCGGTTAG

Upstream 100 bases:

>100_bases
CCATTCGGCTCGAATCGACCGAAGCGGCTGACGCCGTCGCTGGCGCAGCCTAGTGTCTGGTCACAGATCGCCACCGAAAT
CCATGTAGATCGGGGCCCGC

Downstream 100 bases:

>100_bases
TCATATGCCGGGAGCCACATGGTGTCGACTATGGCCTGCCTGACGTCGTCGTGCGTCCTGGAAGCCACCCCGTCTTGGAC
GGCGGCCCGATAGACAGCTT

Product: putative integral membrane transport protein

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLYGRRRWYLIGVS
LFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWG
WRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLV
GRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGS
TIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
LVATATLAVVFLAGWRWFPRDVQTAGSDAKREAAYSDDRRVRG

Sequences:

>Translated_523_residues
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLYGRRRWYLIGVS
LFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWG
WRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLV
GRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGS
TIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
LVATATLAVVFLAGWRWFPRDVQTAGSDAKREAAYSDDRRVRG
>Mature_523_residues
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLYGRRRWYLIGVS
LFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWG
WRSIFYVNLPMGALVLFLTLCYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGLMILPFSAAVAIVSPLVGHLV
GRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGS
TIGFAVLGSVLAAWLSATLEPHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
LVATATLAVVFLAGWRWFPRDVQTAGSDAKREAAYSDDRRVRG

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=403, Percent_Identity=28.5359801488834, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=28.641975308642, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1788710, Length=401, Percent_Identity=29.6758104738155, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=29.5121951219512, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=28.4653465346535, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790146, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082231, Length=175, Percent_Identity=29.7142857142857, Blast_Score=65, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=474, Percent_Identity=23.2067510548523, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324954, Length=393, Percent_Identity=20.8651399491094, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=150, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=26.797385620915, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55690; Mature: 55690

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAATLADLYGRRRWYLIGVSLFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALV
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHH
SAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWGWRSIFYVNLPMGALVLFLTL
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHH
CYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGL
HHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
MILPFSAAVAIVSPLVGHLVGRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGL
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
CGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGSTIGFAVLGSVLAAWLSATLE
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHCCCCCCCEECHHHHHHHHHHHH
LVATATLAVVFLAGWRWFPRDVQTAGSDAKREAAYSDDRRVRG
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNRTQLLTLIATGLGLFMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
SAATLADLYGRRRWYLIGVSLFTLGSIACGLAPSIAVLTTARGAQGLGAAAVSVTSLALV
HHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCHHHHEECCCCCCCCHHHHHHHHHHHH
SAAFPEAKEKARAIGIWTAIASIGTTTGPTLGGLLVDQWGWRSIFYVNLPMGALVLFLTL
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEECCHHHHHHHHHH
CYVEESCNERARRFDLSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALF
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VWLERRSSNPMMDLTLFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTGL
HHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
MILPFSAAVAIVSPLVGHLVGRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGL
EEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
CGSGVALCLTPITTVAMTAVPAERAGMASGIMSAQRAIGSTIGFAVLGSVLAAWLSATLE
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PHLERAVPDPVQRHVLAEIIIDSANPRAHVGGIVPRRHIEHRDPVAIAEEDFIEGIRVAL
HHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCHHHCCCCCCCEECHHHHHHHHHHHH
LVATATLAVVFLAGWRWFPRDVQTAGSDAKREAAYSDDRRVRG
HHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]