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The map label for this gene is moaA

Identifier: 121636792

GI number: 121636792

Start: 996716

End: 997798

Strand: Reverse

Name: moaA

Synonym: BCG_0921c

Alternate gene names: 121636792

Gene position: 997798-996716 (Counterclockwise)

Preceding gene: 121636793

Following gene: 121636791

Centisome position: 22.81

GC content: 69.71

Gene sequence:

>1083_bases
ATGACACTGACCGCGCTGGGCATGCCGGCGCTGCGCAGCCGCACCAACGGCATTGCCGACCCGCGGGTCGTACCCACCAC
TGGCCCTTTGGTGGACACGTTCGGCCGGGTCGCCAACGATCTGCGGGTGTCGCTAACCGACCGCTGCAACCTGCGGTGCA
GCTACTGCATGCCTGAGCGAGGGCTGAGGTGGCTGCCCGGCGAGCAACTGCTGCGGCCCGACGAACTGGCCAGGCTGATA
CACATCGCGGTAACTCGGCTCGGCGTCACCAGCGTGCGGTTCACCGGCGGCGAGCCGCTATTGGCCCACCACCTCGACGA
GGTGGTCGCAGCGACAGCTAGGCTGCGGCCGCGCCCGGAGATCTCGCTGACCACCAACGGGGTGGGACTGGCGCGGCGGG
CGGGCGCCCTGGCCGAAGCGGGCCTGGACCGGGTCAACGTCTCGCTGGATAGCATCGACCGCGCCCACTTCGCTGCTATC
ACCCGTCGGGACCGGCTTGCGCATGTGCTGGCCGGCTTGGCCGCCGCCAAGGCAGCCGGGTTGACACCCGTCAAGGTGAA
CGCCGTCCTCGACCCCACGACCGGCCGCGAGGATGTCGTCGACCTGCTGAGGTTCTGCCTAGAGCGCGGTTATCAATTGC
GAGTCATCGAGCAGATGCCGCTGGACGCGGGGCATAGCTGGCGGCGGAACATCGCACTGAGCGCCGACGACGTGCTGGCG
GCGCTGCGGCCCCACTTCCGGCTACGGCCCGACCCGGCACCGCGCGGTTCGGCCCCGGCCGAACTCTGGCTGGTCGACGC
CGGACCGAACACGCCACGCGGACGGTTCGGTGTCATCGCCTCGGTGTCGCACGCCTTCTGTTCGACGTGCGACCGCACCC
GGCTGACCGCCGATGGCCAGATCCGCAGCTGTCTGTTCTCCACCGAGGAGACCGACCTGCGCCGCCTACTGCGCGGCGGT
GCTGACGACGACGCGATCGAGGCGGCATGGCGTGCCGCGATGTGGAGCAAACCCGCCGGCCACGGCATCAACGCCCCCGA
TTTCATTCAGCCCGACCGCCCGATGAGCGCGATCGGTGGCTAA

Upstream 100 bases:

>100_bases
TCATCGGCATTCCGCTAGCACTGGCCAACTTGAAACTGATCCCGGTGTCGCTGGTGCCGCTGGGCAAGGACATCGTCGGG
GTCAACTCACAGGTGCCCAC

Downstream 100 bases:

>100_bases
CCGGTGACGCAGGTGTCCGACGAATCCGCCGGAATTCAGGTGACTGTCCGCTACTTCGCGGCCGCCCGAGCGGCCGCTGG
TGCCGGGTCGGAGAAAGTCA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTLTALGMPALRSRTNGIADPRVVPTTGPLVDTFGRVANDLRVSLTDRCNLRCSYCMPERGLRWLPGEQLLRPDELARLI
HIAVTRLGVTSVRFTGGEPLLAHHLDEVVAATARLRPRPEISLTTNGVGLARRAGALAEAGLDRVNVSLDSIDRAHFAAI
TRRDRLAHVLAGLAAAKAAGLTPVKVNAVLDPTTGREDVVDLLRFCLERGYQLRVIEQMPLDAGHSWRRNIALSADDVLA
ALRPHFRLRPDPAPRGSAPAELWLVDAGPNTPRGRFGVIASVSHAFCSTCDRTRLTADGQIRSCLFSTEETDLRRLLRGG
ADDDAIEAAWRAAMWSKPAGHGINAPDFIQPDRPMSAIGG

Sequences:

>Translated_360_residues
MTLTALGMPALRSRTNGIADPRVVPTTGPLVDTFGRVANDLRVSLTDRCNLRCSYCMPERGLRWLPGEQLLRPDELARLI
HIAVTRLGVTSVRFTGGEPLLAHHLDEVVAATARLRPRPEISLTTNGVGLARRAGALAEAGLDRVNVSLDSIDRAHFAAI
TRRDRLAHVLAGLAAAKAAGLTPVKVNAVLDPTTGREDVVDLLRFCLERGYQLRVIEQMPLDAGHSWRRNIALSADDVLA
ALRPHFRLRPDPAPRGSAPAELWLVDAGPNTPRGRFGVIASVSHAFCSTCDRTRLTADGQIRSCLFSTEETDLRRLLRGG
ADDDAIEAAWRAAMWSKPAGHGINAPDFIQPDRPMSAIGG
>Mature_359_residues
TLTALGMPALRSRTNGIADPRVVPTTGPLVDTFGRVANDLRVSLTDRCNLRCSYCMPERGLRWLPGEQLLRPDELARLIH
IAVTRLGVTSVRFTGGEPLLAHHLDEVVAATARLRPRPEISLTTNGVGLARRAGALAEAGLDRVNVSLDSIDRAHFAAIT
RRDRLAHVLAGLAAAKAAGLTPVKVNAVLDPTTGREDVVDLLRFCLERGYQLRVIEQMPLDAGHSWRRNIALSADDVLAA
LRPHFRLRPDPAPRGSAPAELWLVDAGPNTPRGRFGVIASVSHAFCSTCDRTRLTADGQIRSCLFSTEETDLRRLLRGGA
DDDAIEAAWRAAMWSKPAGHGINAPDFIQPDRPMSAIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=336, Percent_Identity=35.4166666666667, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI22027536, Length=336, Percent_Identity=35.4166666666667, Blast_Score=199, Evalue=4e-51,
Organism=Escherichia coli, GI1786999, Length=338, Percent_Identity=34.6153846153846, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17561210, Length=337, Percent_Identity=34.7181008902077, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI32566329, Length=337, Percent_Identity=34.4213649851632, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI32566331, Length=319, Percent_Identity=33.5423197492163, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI28574488, Length=342, Percent_Identity=37.4269005847953, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI28574490, Length=338, Percent_Identity=37.2781065088757, Blast_Score=198, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA2_MYCBO (P65385)

Other databases:

- EMBL:   BX248336
- RefSeq:   NP_854550.1
- ProteinModelPortal:   P65385
- SMR:   P65385
- EnsemblBacteria:   EBMYCT00000015022
- GeneID:   1092771
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0893c
- GeneTree:   EBGT00050000014611
- HOGENOM:   HBG529885
- OMA:   FFRAMSR
- ProtClustDB:   PRK00164
- BioCyc:   MBOV233413:MB0893C-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 38986; Mature: 38855

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 42-42 BINDING 55-55 BINDING 93-93 BINDING 97-97 BINDING 124-124 BINDING 148-148 BINDING 185-185 BINDING 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTALGMPALRSRTNGIADPRVVPTTGPLVDTFGRVANDLRVSLTDRCNLRCSYCMPER
CEEECCCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHEEEEECCCCCEEEECCCCC
GLRWLPGEQLLRPDELARLIHIAVTRLGVTSVRFTGGEPLLAHHLDEVVAATARLRPRPE
CCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
ISLTTNGVGLARRAGALAEAGLDRVNVSLDSIDRAHFAAITRRDRLAHVLAGLAAAKAAG
EEEEECCCCHHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTPVKVNAVLDPTTGREDVVDLLRFCLERGYQLRVIEQMPLDAGHSWRRNIALSADDVLA
CCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCEEECCHHHHHH
ALRPHFRLRPDPAPRGSAPAELWLVDAGPNTPRGRFGVIASVSHAFCSTCDRTRLTADGQ
HHCCCCEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCH
IRSCLFSTEETDLRRLLRGGADDDAIEAAWRAAMWSKPAGHGINAPDFIQPDRPMSAIGG
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
TLTALGMPALRSRTNGIADPRVVPTTGPLVDTFGRVANDLRVSLTDRCNLRCSYCMPER
EEECCCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHEEEEECCCCCEEEECCCCC
GLRWLPGEQLLRPDELARLIHIAVTRLGVTSVRFTGGEPLLAHHLDEVVAATARLRPRPE
CCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
ISLTTNGVGLARRAGALAEAGLDRVNVSLDSIDRAHFAAITRRDRLAHVLAGLAAAKAAG
EEEEECCCCHHHHHHHHHHHCCHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LTPVKVNAVLDPTTGREDVVDLLRFCLERGYQLRVIEQMPLDAGHSWRRNIALSADDVLA
CCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHCEEECCHHHHHH
ALRPHFRLRPDPAPRGSAPAELWLVDAGPNTPRGRFGVIASVSHAFCSTCDRTRLTADGQ
HHCCCCEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCH
IRSCLFSTEETDLRRLLRGGADDDAIEAAWRAAMWSKPAGHGINAPDFIQPDRPMSAIGG
HHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972