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The map label for this gene is dus [H]

Identifier: 121636747

GI number: 121636747

Start: 946898

End: 948067

Strand: Reverse

Name: dus [H]

Synonym: BCG_0876c

Alternate gene names: 121636747

Gene position: 948067-946898 (Counterclockwise)

Preceding gene: 121636748

Following gene: 121636746

Centisome position: 21.67

GC content: 67.09

Gene sequence:

>1170_bases
ATGAGCAGGAGGCGGGCAATCCAACCCAGCCCGGCGTTGCGCATCGGCCCGATCGAGCTCGCCAGCCCGGTTGTGCTGGC
TCCGATGGCCGGTGTGACGAACGTCGCATTCCGGGCGCTGTGTCGTCAGCTGGAACAGTCGAAGGTCGGCACGGTCAGCG
GACTGTATGTCTGCGAGATGGTGACCGCACGTGCGCTCATCGAGCGGCACCCGGTCACCATGCACATGACAACGTTCTCC
GCGGACGAATCACCACGCTCGCTGCAGCTGTACACCGTCGATCCGGACACCACGTATGCGGCCGCTCGGATGATCGCTGG
CGAAGGGTTAGCCGATCACATCGACATGAATTTCGGCTGCCCAGTGCCCAAAGTCACCAAGCGCGGCTGCGGGGCGGCGC
TACCGTTCAAACGGCGGCTATTCGGTCAGATCGTGGCCGCGGCTGTGCGCGCCACCGAAGGCACCGATATACCGGTGACG
GTCAAGTTTCGCATTGGCATCGATGACGCCCACCACACACATCTGGATGCCGGCCGCATCGCTGAGGCCGAAGGAGCCGC
TGCGGTCGCGCTGCACGCCCGTACCGCGGCACAACGCTATTCCGGCACCGCCGACTGGGAACAGATCGCCCGGCTCAAGC
AGCATGTCCGGACGATTCCGGTGCTCGGCAACGGCGACATCTACGATGCCGGCGACGCACTGGCCATGATGTCCACCACC
GGCTGTGACGGCGTGGTCATCGGCCGTGGCTGCCTGGGCCGTCCCTGGCTATTTGCCGAGTTGTCCGCGGCTTTCACCGG
CAGCCCGGCCCCCACCCCACCCACGCTCGGCGAAGTCGCCGACATCATCCGCCGTCACGGCACCTTGCTGGCCGCACACT
TCGGCGAGGACAAGGGCATGCGCGATATCCGCAAGCACATCGCCTGGTATCTACATGGCTTCCCGGCGGGCTCCGCGCTC
CGGCGGGCGTTAGCAATGGTCAAGACGCTCGACGAGCTCGACTGCCTGCTGGACCGGTTGGACGGCACGGTACCGTTCCC
GGACTCCGCGACTGGCGCCCGGGGCCGGCAGGGTTCCCCCGCCCGGGTGGCCCTTCCCGACGGCTGGCTAACCGACCCCG
ACGACTGCCGAGTGCCGGAAGGGGCGGACGCCATGGGGTCGGGTGGCTGA

Upstream 100 bases:

>100_bases
GAGCCGTCGTTAGCCCGGCGACGATGCAGAGCGCGCAGCGCGATGAGCAGGAGGCGGGCAATCCAACCCAGCCCGGCGAC
GATGCAGAGCGCGCAGCGCG

Downstream 100 bases:

>100_bases
ATCGAGACCCTGTCGAAATCGCGTCTGGACCGATAAATCAGTACGATTTGGGCCTTGCTGCATTGTGCGGCGCCGACTCG
TGGCGGTGCTGCGCGAAGGT

Product: putative transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFS
ADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGCGAALPFKRRLFGQIVAAAVRATEGTDIPVT
VKFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSAL
RRALAMVKTLDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG

Sequences:

>Translated_389_residues
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFS
ADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGCGAALPFKRRLFGQIVAAAVRATEGTDIPVT
VKFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSAL
RRALAMVKTLDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG
>Mature_388_residues
SRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEMVTARALIERHPVTMHMTTFSA
DESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGCPVPKVTKRGCGAALPFKRRLFGQIVAAAVRATEGTDIPVTV
KFRIGIDDAHHTHLDAGRIAEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTTG
CDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGMRDIRKHIAWYLHGFPAGSALR
RALAMVKTLDELDCLLDRLDGTVPFPDSATGARGRQGSPARVALPDGWLTDPDDCRVPEGADAMGSGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=306, Percent_Identity=30.0653594771242, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239788462, Length=302, Percent_Identity=30.1324503311258, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI31742496, Length=235, Percent_Identity=30.6382978723404, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI40807366, Length=318, Percent_Identity=27.9874213836478, Blast_Score=86, Evalue=7e-17,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=32.8125, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=30.7692307692308, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=26.9230769230769, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=291, Percent_Identity=25.7731958762887, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI25144369, Length=233, Percent_Identity=30.9012875536481, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=323, Percent_Identity=23.8390092879257, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=240, Percent_Identity=26.25, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=293, Percent_Identity=27.3037542662116, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19921524, Length=238, Percent_Identity=29.4117647058824, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24580595, Length=273, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920448, Length=273, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 41401; Mature: 41270

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEM
CCCCCCCCCCCCEEECCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VTARALIERHPVTMHMTTFSADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGC
HHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCC
PVPKVTKRGCGAALPFKRRLFGQIVAAAVRATEGTDIPVTVKFRIGIDDAHHTHLDAGRI
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
AEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
CCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCEECCCCCEEEEECC
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGM
CCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCH
RDIRKHIAWYLHGFPAGSALRRALAMVKTLDELDCLLDRLDGTVPFPDSATGARGRQGSP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARVALPDGWLTDPDDCRVPEGADAMGSGG
CEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRRRAIQPSPALRIGPIELASPVVLAPMAGVTNVAFRALCRQLEQSKVGTVSGLYVCEM
CCCCCCCCCCCEEECCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VTARALIERHPVTMHMTTFSADESPRSLQLYTVDPDTTYAAARMIAGEGLADHIDMNFGC
HHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCCC
PVPKVTKRGCGAALPFKRRLFGQIVAAAVRATEGTDIPVTVKFRIGIDDAHHTHLDAGRI
CCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCC
AEAEGAAAVALHARTAAQRYSGTADWEQIARLKQHVRTIPVLGNGDIYDAGDALAMMSTT
CCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEECCCCEECCCCCEEEEECC
GCDGVVIGRGCLGRPWLFAELSAAFTGSPAPTPPTLGEVADIIRRHGTLLAAHFGEDKGM
CCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCH
RDIRKHIAWYLHGFPAGSALRRALAMVKTLDELDCLLDRLDGTVPFPDSATGARGRQGSP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ARVALPDGWLTDPDDCRVPEGADAMGSGG
CEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]