Click here to switch to the map view.

The map label for this gene is cysA2 [H]

Identifier: 121636739

GI number: 121636739

Start: 938907

End: 939740

Strand: Reverse

Name: cysA2 [H]

Synonym: BCG_0867c

Alternate gene names: 121636739

Gene position: 939740-938907 (Counterclockwise)

Preceding gene: 121636740

Following gene: 121636738

Centisome position: 21.48

GC content: 61.75

Gene sequence:

>834_bases
ATGGCACGCTGCGATGTCCTGGTCTCCGCCGACTGGGCTGAGAGCAATCTGCACGCGCCGAAGGTCGTTTTCGTCGAAGT
GGACGAGGACACCAGTGCATATGACCGTGACCATATTGCCGGCGCGATCAAGTTGGACTGGCGCACCGACCTGCAGGATC
CGGTCAAACGTGACTTCGTCGACGCCCAGCAATTCTCCAAGCTGCTGTCCGAGCGTGGCATCGCCAACGAGGACACGGTG
ATCCTGTACGGCGGCAACAACAATTGGTTCGCCGCCTACGCGTACTGGTATTTCAAGCTCTACGGCCATGAGAAGGTCAA
GTTGCTCGACGGCGGCCGCAAGAAGTGGGAGCTCGACGGACGCCCGCTGTCCAGCGACCCGGTCAGCCGGCCGGTGACCT
CCTACACCGCCTCCCCGCCGGATAACACGATTCGGGCATTCCGCGACGAGGTCCTGGCGGCCATCAACGTCAAGAACCTC
ATCGACGTGCGCTCTCCCGACGAGTTCTCCGGCAAGATCCTGGCCCCCGCGCACCTGCCGCAGGAACAAAGCCAGCGGCC
CGGACACATTCCTGGTGCCATCAACGTGCCGTGGAGCAGGGCCGCCAACGAGGACGGCACCTTCAAGTCCGATGAGGAGT
TGGCCAAGCTTTACGCCGACGCCGGCCTAGACAACAGCAAGGAAACGATTGCCTACTGCCGAATCGGGGAACGGTCCTCG
CACACCTGGTTCGTGTTGCGGGAATTACTCGGACACCAAAACGTCAAGAACTACGACGGCAGTTGGACAGAATACGGCTC
CCTGGTGGGCGCCCCGATCGAGTTGGGAAGCTGA

Upstream 100 bases:

>100_bases
TGCAACAGACGTCCCAAGGAGCAGTCAAGCAGTAACACTCCCCGTCCCGCCTAACCAGTTGACCACGCTGGTCACCGAAC
TATGCGAAAGGATTCCCGCC

Downstream 100 bases:

>100_bases
TATGTGCTCTGGACCCAAGCAAGGACTGACATTGCCGGCCAGCGTCGACCTGGAAAAAGAAACGGTGATCACCGGCCGCG
TAGTGGACGGTGACGGCCAG

Product: putative thiosulfate sulfurtransferase cysA2

Products: NA

Alternate protein names: Rhodanese-like protein 2 [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFVDAQQFSKLLSERGIANEDTV
ILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDGRPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNL
IDVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HTWFVLRELLGHQNVKNYDGSWTEYGSLVGAPIELGS

Sequences:

>Translated_277_residues
MARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFVDAQQFSKLLSERGIANEDTV
ILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDGRPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNL
IDVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HTWFVLRELLGHQNVKNYDGSWTEYGSLVGAPIELGS
>Mature_276_residues
ARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFVDAQQFSKLLSERGIANEDTVI
LYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDGRPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNLI
DVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSSH
TWFVLRELLGHQNVKNYDGSWTEYGSLVGAPIELGS

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=243, Percent_Identity=30.8641975308642, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI194473668, Length=280, Percent_Identity=27.8571428571429, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI61835204, Length=280, Percent_Identity=27.8571428571429, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI194473681, Length=280, Percent_Identity=27.8571428571429, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=28.5714285714286, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081967, Length=279, Percent_Identity=26.5232974910394, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=261, Percent_Identity=28.735632183908, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71997283, Length=248, Percent_Identity=29.4354838709677, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115534702, Length=249, Percent_Identity=29.718875502008, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543836, Length=220, Percent_Identity=26.8181818181818, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17543838, Length=220, Percent_Identity=26.8181818181818, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17559150, Length=271, Percent_Identity=25.830258302583, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001128
- InterPro:   IPR017972
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 31015; Mature: 30884

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFV
CCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHC
DAQQFSKLLSERGIANEDTVILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDG
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEECC
RPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNLIDVRSPDEFSGKILAPAHLP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHEECCCCCCCCCEEECCCCCC
QEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
HTWFVLRELLGHQNVKNYDGSWTEYGSLVGAPIELGS
CHHHHHHHHHCCCCCCCCCCCHHHHCCEECCCEECCC
>Mature Secondary Structure 
ARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFV
CCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHC
DAQQFSKLLSERGIANEDTVILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDG
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEECC
RPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNLIDVRSPDEFSGKILAPAHLP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHEECCCCCCCCCEEECCCCCC
QEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
HTWFVLRELLGHQNVKNYDGSWTEYGSLVGAPIELGS
CHHHHHHHHHCCCCCCCCCCCHHHHCCEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]