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The map label for this gene is merA [H]

Identifier: 121636720

GI number: 121636720

Start: 919518

End: 920417

Strand: Reverse

Name: merA [H]

Synonym: BCG_0848c

Alternate gene names: 121636720

Gene position: 920417-919518 (Counterclockwise)

Preceding gene: 121636722

Following gene: 121636719

Centisome position: 21.04

GC content: 68.78

Gene sequence:

>900_bases
ATGACCGCGGCCCAACAGGACCAGGCGCCAATGGCAACACCCGGCTGCCGTGAGGGTGAAACGTATGACGTCGTCGTGCT
CGGCGCGGGACCCGTTGGACAGAACGTCGCCGATCGTGCCCGCGCGGGGGGCCTGCGTGTCGCGGTGGTGGAGCGCGAAC
TCGTCGGGGGTGAATGCTCCTATTGGGCCTGTGTGCCCAGCAAAGCCTTGCTGCGTCCGGTCATCGCGATCTCTGACGCC
CGACGGGTCGACGGCGCGCGCGAAGCAGTCGACGGCTCGATCAACACAGCCGGCGTCTTTGGCCGCCGCAACCGCTATGT
GGCCCACTGGGACGACACCGGCCAGGCCGACTGGGTGAGTGGAATCGGCGCGACGCTGATACGCGGTGACGGGCGATTGG
ACGGTCCGCGCCGCGTCGTCGTCACCAAGTCGAGCGGCGAAAGCGTGGCGCTGACCGCCCGGCATGCCGTTGTCATCTGC
ACCGGAAGCCGGCCAGCACTCCCCGACCTTCCTGGCATCACCGAAGCCCGGCCATGGACCAATCGCCAAGCCACCGACAA
CAGTACGGTCCCCGACCGGCTTGCGATCGTCGGCGCCGGCGGCGTCGGTGTGGAGATGGCGACCGCCTGGCAGGGACTGG
GCGCCTCGGTGACCCTGCTGGCTCGGGGATCTGGCCTGCTGCCCCGAATGGAACCGTTTGTGGGGGAACTCATCGGTCGC
GGACTGGCCGACGCCGGCGTTGACGTGCGCGTGGGAGTATCGGTACGCGCGCTGGGCCGCCCCAACCCACTGGCCCAGTG
GTCCTCGAGCTGGACGACGGTACCGAGCTGCGGGTCGACGAGGTACTCTTCGCCACCGGCCGAGCACCGCGAACCGACGA
CATCGGCTTGGAGACAATAG

Upstream 100 bases:

>100_bases
TCGAGAGCTTTTGCGTCTGGTGGGCGATAGGCCGGCACGGCTCACCGGCGCTAGGCGCGCGTAGCGTCGCTGGCAGAGTC
CGACGAAAGGATCTTTGATT

Downstream 100 bases:

>100_bases
GACTGACGCCGGGCAGCTGGCTGGACGTCGATGACACCTGCCGAGTGCGGGCTGTTGACGACGGCTGGCTCTATGCCGCC
GGCGACGTCAACCATCGCGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECSYWACVPSKALLRPVIAISDA
RRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVSGIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVIC
TGSRPALPDLPGITEARPWTNRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
GLADAGVDVRVGVSVRALGRPNPLAQWSSSWTTVPSCGSTRYSSPPAEHREPTTSAWRQ

Sequences:

>Translated_299_residues
MTAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECSYWACVPSKALLRPVIAISDA
RRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVSGIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVIC
TGSRPALPDLPGITEARPWTNRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
GLADAGVDVRVGVSVRALGRPNPLAQWSSSWTTVPSCGSTRYSSPPAEHREPTTSAWRQ
>Mature_298_residues
TAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECSYWACVPSKALLRPVIAISDAR
RVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVSGIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVICT
GSRPALPDLPGITEARPWTNRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGRG
LADAGVDVRVGVSVRALGRPNPLAQWSSSWTTVPSCGSTRYSSPPAEHREPTTSAWRQ

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=202, Percent_Identity=30.6930693069307, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI50301238, Length=244, Percent_Identity=27.0491803278689, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786307, Length=217, Percent_Identity=30.8755760368664, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87082354, Length=240, Percent_Identity=27.5, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789915, Length=204, Percent_Identity=28.921568627451, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081717, Length=260, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32565766, Length=201, Percent_Identity=30.3482587064677, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358499, Length=249, Percent_Identity=28.5140562248996, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 31296; Mature: 31164

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECS
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCEEEEEEEHHHCCCCCC
YWACVPSKALLRPVIAISDARRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVS
EEEECCCHHHHHHHHHHCCCHHCCCHHHHHCCCCCCEEECCCCCCEEEECCCCCCCHHHH
GIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVICTGSRPALPDLPGITEARPWT
CCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCC
NRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
CCCCCCCCCCCCEEEEEECCCCCEEHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHC
GLADAGVDVRVGVSVRALGRPNPLAQWSSSWTTVPSCGSTRYSSPPAEHREPTTSAWRQ
CCHHCCCEEEECEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHCC
>Mature Secondary Structure 
TAAQQDQAPMATPGCREGETYDVVVLGAGPVGQNVADRARAGGLRVAVVERELVGGECS
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHCCCEEEEEEEHHHCCCCCC
YWACVPSKALLRPVIAISDARRVDGAREAVDGSINTAGVFGRRNRYVAHWDDTGQADWVS
EEEECCCHHHHHHHHHHCCCHHCCCHHHHHCCCCCCEEECCCCCCEEEECCCCCCCHHHH
GIGATLIRGDGRLDGPRRVVVTKSSGESVALTARHAVVICTGSRPALPDLPGITEARPWT
CCCEEEEECCCCCCCCEEEEEECCCCCEEEEEECEEEEEEECCCCCCCCCCCCCCCCCCC
NRQATDNSTVPDRLAIVGAGGVGVEMATAWQGLGASVTLLARGSGLLPRMEPFVGELIGR
CCCCCCCCCCCCEEEEEECCCCCEEHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHC
GLADAGVDVRVGVSVRALGRPNPLAQWSSSWTTVPSCGSTRYSSPPAEHREPTTSAWRQ
CCHHCCCEEEECEEEEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3037534 [H]