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The map label for this gene is nrdB

Identifier: 121636145

GI number: 121636145

Start: 306615

End: 307559

Strand: Direct

Name: nrdB

Synonym: BCG_0270

Alternate gene names: NA

Gene position: 306615-307559 (Clockwise)

Preceding gene: 121636144

Following gene: 121636150

Centisome position: 7.01

GC content: 64.87

Gene sequence:

>945_bases
ATGACACGCACACGTTCGGGCTCGCTCGCCGCGGGCGGACTCAACTGGGCGAGCCTGCCACTGAAGCTGTTCGCCGGGGG
CAACGCAAAGTTCTGGGATCCGGCCGACATCGACTTCACGCGCGACCGGGCGGACTGGGAGAAGTTGTCGGACGACGAAC
GTGACTACGCCACCCGATTGTGCACCCAGTTCATTGCCGGCGAGGAGGCGGTGACCGAGGACATCCAGCCGTTCATGTCC
GCGATGCGGGCCGAGGGACGGCTGGCCGACGAGATGTATCTGACGCAGTTCGCGTTCGAGGAAGCCAAACACACCCAGGT
GTTTCGCATGTGGCTGGATGCCGTCGGAATCAGCGAAGACTTGCATCGCTATCTCGACGACTTGCCCGCCTACCGCCAAA
TCTTCTACGCGGAGTTGCCGGAGTGCCTCAACGCATTGTCGGCCGATCCCTCACCGGCCGCCCAGGTCCGGGCGTCGGTC
ACCTACAACCACATCGTGGAAGGCATGCTGGCGCTCACGGGCTACTACGCCTGGCACAAGATCTGTGTGGAACGCGCAAT
CCTTCCCGGCATGCAGGAGCTGGTCCGGCGCATCGGTGACGACGAGCGACGCCACATGGCTTGGGGCACCTTCACCTGTC
GGCGCCACGTCGCCGCCGACGACGCCAATTGGACGGTGTTCGAAACACGGATGAACGAGCTCATCCCGCTGGCGCTGCGC
CTCATCGAGGAGGGCTTTGCGCTGTACGGCGACCAGCCCCCATTCGACCTGTCCAAGGACGATTTCCTGCAATACTCGAC
CGACAAGGGAATGCGCCGGTTCGGCACCATCAGCAACGCCCGCGGCCGGCCGGTCGCCGAAATCGACGTCGACTACTCGC
CCGCGCAGCTGGAGGACACCTTCGCCGACGAGGACCGGCGCACCCTGGCAGCGGCCTCGGCCTAG

Upstream 100 bases:

>100_bases
CCGCGCTGGCCGTGCGCAGGCTTGTCGCCGTGCTGGCGGCGGCCTTCGATGCCCAGCACCCCGCGACCAACTCAGCCCAG
ACCCGATCGGAGGAGATCAC

Downstream 100 bases:

>100_bases
GCCTGGCGAGCAGACGCAAAATCGCCCAATTTCGTGCCGAATTGGGCGATTTTGCGTCTGCTCGCCAGGGGAACGCTAGG
CGATCCAGACGGTCTTGATG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: ribonucleoside diphosphate; thioredoxin

Alternate protein names: Ribonucleotide reductase R2 subunit homolog; Ribonucleotide reductase small subunit homolog

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTRTRSGSLAAGGLNWASLPLKLFAGGNAKFWDPADIDFTRDRADWEKLSDDERDYATRLCTQFIAGEEAVTEDIQPFMS
AMRAEGRLADEMYLTQFAFEEAKHTQVFRMWLDAVGISEDLHRYLDDLPAYRQIFYAELPECLNALSADPSPAAQVRASV
TYNHIVEGMLALTGYYAWHKICVERAILPGMQELVRRIGDDERRHMAWGTFTCRRHVAADDANWTVFETRMNELIPLALR
LIEEGFALYGDQPPFDLSKDDFLQYSTDKGMRRFGTISNARGRPVAEIDVDYSPAQLEDTFADEDRRTLAAASA

Sequences:

>Translated_314_residues
MTRTRSGSLAAGGLNWASLPLKLFAGGNAKFWDPADIDFTRDRADWEKLSDDERDYATRLCTQFIAGEEAVTEDIQPFMS
AMRAEGRLADEMYLTQFAFEEAKHTQVFRMWLDAVGISEDLHRYLDDLPAYRQIFYAELPECLNALSADPSPAAQVRASV
TYNHIVEGMLALTGYYAWHKICVERAILPGMQELVRRIGDDERRHMAWGTFTCRRHVAADDANWTVFETRMNELIPLALR
LIEEGFALYGDQPPFDLSKDDFLQYSTDKGMRRFGTISNARGRPVAEIDVDYSPAQLEDTFADEDRRTLAAASA
>Mature_313_residues
TRTRSGSLAAGGLNWASLPLKLFAGGNAKFWDPADIDFTRDRADWEKLSDDERDYATRLCTQFIAGEEAVTEDIQPFMSA
MRAEGRLADEMYLTQFAFEEAKHTQVFRMWLDAVGISEDLHRYLDDLPAYRQIFYAELPECLNALSADPSPAAQVRASVT
YNHIVEGMLALTGYYAWHKICVERAILPGMQELVRRIGDDERRHMAWGTFTCRRHVAADDANWTVFETRMNELIPLALRL
IEEGFALYGDQPPFDLSKDDFLQYSTDKGMRRFGTISNARGRPVAEIDVDYSPAQLEDTFADEDRRTLAAASA

Specific function: Probable oxidase that might be involved in lipid metabolism

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family. R2-like ligand binding oxidase subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIR2H_MYCBO (Q7U2I1)

Other databases:

- EMBL:   BX248334
- RefSeq:   NP_853903.1
- ProteinModelPortal:   Q7U2I1
- EnsemblBacteria:   EBMYCT00000015687
- GeneID:   1090987
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0238
- GeneTree:   EBGT00050000016101
- HOGENOM:   HBG362860
- OMA:   CAEFIAG
- ProtClustDB:   PRK08326
- BioCyc:   MBOV233413:MB0238-MONOMER
- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358
- Gene3D:   G3DSA:1.10.620.20
- PANTHER:   PTHR23409

Pfam domain/function: PF00268 Ribonuc_red_sm; SSF47240 Ferritin/RR_like

EC number: 1.17.4.1

Molecular weight: Translated: 35634; Mature: 35503

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTRSGSLAAGGLNWASLPLKLFAGGNAKFWDPADIDFTRDRADWEKLSDDERDYATRL
CCCCCCCCEEECCCCHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHH
CTQFIAGEEAVTEDIQPFMSAMRAEGRLADEMYLTQFAFEEAKHTQVFRMWLDAVGISED
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LHRYLDDLPAYRQIFYAELPECLNALSADPSPAAQVRASVTYNHIVEGMLALTGYYAWHK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICVERAILPGMQELVRRIGDDERRHMAWGTFTCRRHVAADDANWTVFETRMNELIPLALR
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
LIEEGFALYGDQPPFDLSKDDFLQYSTDKGMRRFGTISNARGRPVAEIDVDYSPAQLEDT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
FADEDRRTLAAASA
HCCCHHHHHHHCCC
>Mature Secondary Structure 
TRTRSGSLAAGGLNWASLPLKLFAGGNAKFWDPADIDFTRDRADWEKLSDDERDYATRL
CCCCCCCEEECCCCHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHH
CTQFIAGEEAVTEDIQPFMSAMRAEGRLADEMYLTQFAFEEAKHTQVFRMWLDAVGISED
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LHRYLDDLPAYRQIFYAELPECLNALSADPSPAAQVRASVTYNHIVEGMLALTGYYAWHK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICVERAILPGMQELVRRIGDDERRHMAWGTFTCRRHVAADDANWTVFETRMNELIPLALR
HHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHH
LIEEGFALYGDQPPFDLSKDDFLQYSTDKGMRRFGTISNARGRPVAEIDVDYSPAQLEDT
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHH
FADEDRRTLAAASA
HCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2'-deoxyribonucleoside diphosphate; thioredoxin disulfide; H2O

Specific reaction: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972