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The map label for this gene is php [C]

Identifier: 121636142

GI number: 121636142

Start: 303013

End: 303993

Strand: Reverse

Name: php [C]

Synonym: BCG_0267c

Alternate gene names: 121636142

Gene position: 303993-303013 (Counterclockwise)

Preceding gene: 121636146

Following gene: 121636141

Centisome position: 6.95

GC content: 65.75

Gene sequence:

>981_bases
GTGCCAGAACTAAATACCGCTCGCGGACCCATCGACACCGCTGATCTCGGCGTCACGCTGATGCACGAGCACGTCTTCAT
CATGACCACCGAGATTGCGCAGAACTACCCGGAAGCCTGGGGCGACGAGGACAAGCGGGTGGCCGGCGCCATCGCCCGGC
TAGGCGAACTCAAGGCCCGCGGCGTGGACACCATCGTCGACCTCACGGTGATCGGGCTGGGCCGATACATCCCGCGCATC
GCCCGGGTGGCCGCGGCCACCGAGCTGAACATCGTCGTGGCCACCGGCTTGTACACCTACAACGACGTCCCGTTCTACTT
CCACTACCTCGGGCCGGGCGCACAGCTGGACGGCCCGGAGATCATGACCGACATGTTCGTCCGCGACATCGAGCACGGCA
TCGCCGACACCGGCATCAAGGCGGGAATCCTCAAGTGCGCCACCGACGAACCCGGCCTCACCCCTGGTGTCGAGCGGGTG
TTGCGCGCGGTCGCCCAAGCACACAAACGCACCGGGGCGCCGATCTCCACCCACACCCACGCCGGGCTGCGGCGCGGCCT
TGACCAGCAACGCATCTTCGCCGAGGAGGGGGTGGAACTGAGCCGGGTGGTTATCGGACACTGCGGCGACAGCACCGACG
TCGGCTACCTGGAAGAGCTCATCGCCGCCGGCTCCTACCTCGGGATGGACCGGTTCGGCGTCGACGTGATCTCACCGTTT
CAGGACCGGGTGAACATCGTGGCCCGAATGTGCGAGCGCGGCCATGCCGACAAGATGGTGCTATCACACGACGCCTGCTG
CTATTTCGACGCGCTTCCCGAGGAGCTGGTGCCGGTGGCGATGCCGAATTGGCATTACCTCCACATCCACAACGACGTCA
TCCCCGCACTGAAGCAGCACGGCGTCACCGACGAGCAGCTGCACACCATGCTCGTCGACAACCCGCGCCGCATCTTCGAG
CGGCAGGGCGGCTATCAGTGA

Upstream 100 bases:

>100_bases
CAGCATAAGAATTGGCTCCCGAGCGGTTCGGTCAACGACGAGGGTGACGCTACTGCACTGCGGCCCCGCAAGACACCGAT
TGCGGCCTAGCATCAGTGAC

Downstream 100 bases:

>100_bases
GACAGCCGCGCCGGGCGAATGCCATGGGCTTGGCATTGTGCATATATATCGGCTCCTTATTGATATATACTCCGATTCAT
GGCGAAACATCTCGTCGACA

Product: putative phosphotriesterase PHP

Products: dialkyl phosphate; aryl alcohol

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKARGVDTIVDLTVIGLGRYIPRI
ARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPEIMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERV
LRAVAQAHKRTGAPISTHTHAGLRRGLDQQRIFAEEGVELSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQHGVTDEQLHTMLVDNPRRIFE
RQGGYQ

Sequences:

>Translated_326_residues
MPELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKARGVDTIVDLTVIGLGRYIPRI
ARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPEIMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERV
LRAVAQAHKRTGAPISTHTHAGLRRGLDQQRIFAEEGVELSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQHGVTDEQLHTMLVDNPRRIFE
RQGGYQ
>Mature_325_residues
PELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKARGVDTIVDLTVIGLGRYIPRIA
RVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPEIMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERVL
RAVAQAHKRTGAPISTHTHAGLRRGLDQQRIFAEEGVELSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPFQ
DRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQHGVTDEQLHTMLVDNPRRIFER
QGGYQ

Specific function: Its Real Enzymatic Activity Is Not Yet Known. It Was Tested For General Esterase, Aminopeptidase, Sulfatase, Phosphatase, Carbonic Anhydrase, Phosphodiesterase, And Phosphotriesterase Activities With The Following Substrates: P- Nitrophenyl Acetate, L-Ala

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=352, Percent_Identity=24.4318181818182, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI47933341, Length=352, Percent_Identity=24.4318181818182, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789780, Length=305, Percent_Identity=30.4918032786885, Blast_Score=122, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645325, Length=360, Percent_Identity=23.3333333333333, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: 3.1.8.1

Molecular weight: Translated: 35900; Mature: 35769

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS01322 PHOSPHOTRIESTERASE_1 ; PS01323 PHOSPHOTRIESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKAR
CCCCCCCCCCCCHHHCCCEEECCEEEEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHC
GVDTIVDLTVIGLGRYIPRIARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECEEEECCCCEEEEECCCCCCCCCHH
IMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERVLRAVAQAHKRTGAPISTHTH
HHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
AGLRRGLDQQRIFAEEGVELSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
HHHHHCCCHHHHHHHCCCCEEHHEEECCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHH
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQH
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCHHHHCCCCCCCEEEEEECCHHHHHHHHC
GVTDEQLHTMLVDNPRRIFERQGGYQ
CCCHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure 
PELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKAR
CCCCCCCCCCCHHHCCCEEECCEEEEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHC
GVDTIVDLTVIGLGRYIPRIARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECEEEECCCCEEEEECCCCCCCCCHH
IMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERVLRAVAQAHKRTGAPISTHTH
HHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
AGLRRGLDQQRIFAEEGVELSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
HHHHHCCCHHHHHHHCCCCEEHHEEECCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHH
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQH
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCHHHHCCCCCCCEEEEEECCHHHHHHHHC
GVTDEQLHTMLVDNPRRIFERQGGYQ
CCCHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: aryl dialkyl phosphate; H2O

Specific reaction: An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]