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The map label for this gene is amaB [H]

Identifier: 120613382

GI number: 120613382

Start: 5302709

End: 5303968

Strand: Direct

Name: amaB [H]

Synonym: Aave_4755

Alternate gene names: 120613382

Gene position: 5302709-5303968 (Clockwise)

Preceding gene: 120613381

Following gene: 120613383

Centisome position: 99.06

GC content: 73.1

Gene sequence:

>1260_bases
GTGGACATAACAATCTCTCCCCCCGCGCTGCAGTACCGCGTGAACGGCGCGCGGCTGTGGCAGTCGCTGATGGACCTGGC
GCGCATCGGCGCCACGCCGAAGGGCGGCGTGTGCCGGCTGGCGCTCACCGCGCTGGACGGCGAGGGCCGCGACCTGTTCG
TGCGCTGGGCGCGCGAGGCCGGCTGCAGCGTGCGCATCGACGCCGTCGGCAACATCTTCGCGCGCCGCGCCGGGCGCGAC
GACGCCCTGCCGCCCGTGATGACCGGCAGCCACATCGACACCCAGCCCACCGGCGGCAAGTTCGATGGCAACTACGGCGT
GCTGGCCGGCCTGGAGGTGGTGCGCAGCCTGAACGATGCGGGCATCGAGACCGAGGCCCCCATCGAGGTCGCGGTGTGGA
CCAACGAGGAAGGCTCGCGCTTCGTGCCCGTGATGATGGGCTCGGGCGTGTTCGCCGGCGCCTTCACCCTGGAGCATGCG
CTCGCGCAGCGCGACGGCCACGGCACCAGCGTGGCGGAGGCGCTGGCCGCCATCGGCTATGCCGGCCAGGCGGTGTCCAC
GCCCGCGGTGGGCGCGTACTTCGAGGCGCACATCGAGCAGGGGCCCGTGCTGGAAACCAACGGCTGCGTGATCGGCGTGG
TGCGGGGCGCCCTCGGGCAGCGCTGGTACGACGTGACCGTGCAGGGCATGGAAGCCCACGCGGGCCCCACGCCCATGGAC
CTGCGCCGCGACGCGCTGCTGGCCGCCGGCAGCCTGGTGGAAGAGGTGAACCGCATCGCCCTGGCGCACGCGCCGCACGC
GCGCGGCACGGTGGGCGTGCTGGAGGTGTTTCCGAGTTCCCGCAACGTGATCCCCGGCAGCGTGCGCATGACCGTGGACC
TGCGCGCCGCCGACGACGCGGTGCTGCTGCAGATGGACGCCGCCCTGCGCGAAGCCTGCGAGCGCATCGGCGCCGCCCGC
CGCACGCCGGTGGCGGTGGAGCAGGTGGTGTACTTCCCCCCGCAGCCCTTCACCCCCGAGCTGGTCGAAGGTGTGCGCGC
CGACGCGGCTGCGCTGGGCTACAGCGCCATGGACGTGGTGAGCGGCGCCGGGCACGACGCCGTATACCTGGCGCGCCTGG
CCCCCACGGCCATGGTCTTCGTGCCGTGCGCGGACGGCATCAGCCACAACGAGATCGAGGACGCCGAACCCGAACACCTG
GAAGCCGGCTGCAACGTGCTGCTGCACGCCATGCTGCGCAGCGCGGGGGTGGCCGCATGA

Upstream 100 bases:

>100_bases
AGGCCTTCCTGGCCGACGAAGCGACCAAGGACCGATACCTGGGCGTGGGCCTCGCCTAGAGTCGGCTCTCCGCCCCCCAA
CCCCTTCAGGATCACCGACC

Downstream 100 bases:

>100_bases
CGATGAAGTTCCTGATCGCGCGCCTCAACCACGAGACCAACACCTTCTCGCCGGTGCCCACGCCGCTGGAAGCCTTCTCG
CCCCGCTACGGGCAGGAGGC

Product: amidase

Products: NA

Alternate protein names: L-carbamoylase [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDITISPPALQYRVNGARLWQSLMDLARIGATPKGGVCRLALTALDGEGRDLFVRWAREAGCSVRIDAVGNIFARRAGRD
DALPPVMTGSHIDTQPTGGKFDGNYGVLAGLEVVRSLNDAGIETEAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLEHA
LAQRDGHGTSVAEALAAIGYAGQAVSTPAVGAYFEAHIEQGPVLETNGCVIGVVRGALGQRWYDVTVQGMEAHAGPTPMD
LRRDALLAAGSLVEEVNRIALAHAPHARGTVGVLEVFPSSRNVIPGSVRMTVDLRAADDAVLLQMDAALREACERIGAAR
RTPVAVEQVVYFPPQPFTPELVEGVRADAAALGYSAMDVVSGAGHDAVYLARLAPTAMVFVPCADGISHNEIEDAEPEHL
EAGCNVLLHAMLRSAGVAA

Sequences:

>Translated_419_residues
MDITISPPALQYRVNGARLWQSLMDLARIGATPKGGVCRLALTALDGEGRDLFVRWAREAGCSVRIDAVGNIFARRAGRD
DALPPVMTGSHIDTQPTGGKFDGNYGVLAGLEVVRSLNDAGIETEAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLEHA
LAQRDGHGTSVAEALAAIGYAGQAVSTPAVGAYFEAHIEQGPVLETNGCVIGVVRGALGQRWYDVTVQGMEAHAGPTPMD
LRRDALLAAGSLVEEVNRIALAHAPHARGTVGVLEVFPSSRNVIPGSVRMTVDLRAADDAVLLQMDAALREACERIGAAR
RTPVAVEQVVYFPPQPFTPELVEGVRADAAALGYSAMDVVSGAGHDAVYLARLAPTAMVFVPCADGISHNEIEDAEPEHL
EAGCNVLLHAMLRSAGVAA
>Mature_419_residues
MDITISPPALQYRVNGARLWQSLMDLARIGATPKGGVCRLALTALDGEGRDLFVRWAREAGCSVRIDAVGNIFARRAGRD
DALPPVMTGSHIDTQPTGGKFDGNYGVLAGLEVVRSLNDAGIETEAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLEHA
LAQRDGHGTSVAEALAAIGYAGQAVSTPAVGAYFEAHIEQGPVLETNGCVIGVVRGALGQRWYDVTVQGMEAHAGPTPMD
LRRDALLAAGSLVEEVNRIALAHAPHARGTVGVLEVFPSSRNVIPGSVRMTVDLRAADDAVLLQMDAALREACERIGAAR
RTPVAVEQVVYFPPQPFTPELVEGVRADAAALGYSAMDVVSGAGHDAVYLARLAPTAMVFVPCADGISHNEIEDAEPEHL
EAGCNVLLHAMLRSAGVAA

Specific function: Involved In The Anaerobic Utilization Of Allantoin. Converts Allantoate To (S)-Ureidoglycolate And Ammonia. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI1786726, Length=396, Percent_Identity=34.5959595959596, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010158
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.87 [H]

Molecular weight: Translated: 44013; Mature: 44013

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDITISPPALQYRVNGARLWQSLMDLARIGATPKGGVCRLALTALDGEGRDLFVRWAREA
CCEEECCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEHHHC
GCSVRIDAVGNIFARRAGRDDALPPVMTGSHIDTQPTGGKFDGNYGVLAGLEVVRSLNDA
CCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GIETEAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLEHALAQRDGHGTSVAEALAAIGY
CCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
AGQAVSTPAVGAYFEAHIEQGPVLETNGCVIGVVRGALGQRWYDVTVQGMEAHAGPTPMD
CCCCCCCCCHHHHHHHHCCCCCEEECCCEEEEEEHHHHCCEEEEEEEEEEECCCCCCCHH
LRRDALLAAGSLVEEVNRIALAHAPHARGTVGVLEVFPSSRNVIPGSVRMTVDLRAADDA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCE
VLLQMDAALREACERIGAARRTPVAVEQVVYFPPQPFTPELVEGVRADAAALGYSAMDVV
EEEEHHHHHHHHHHHHCCCCCCCCEEEHEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHH
SGAGHDAVYLARLAPTAMVFVPCADGISHNEIEDAEPEHLEAGCNVLLHAMLRSAGVAA
CCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDITISPPALQYRVNGARLWQSLMDLARIGATPKGGVCRLALTALDGEGRDLFVRWAREA
CCEEECCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEHHHC
GCSVRIDAVGNIFARRAGRDDALPPVMTGSHIDTQPTGGKFDGNYGVLAGLEVVRSLNDA
CCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GIETEAPIEVAVWTNEEGSRFVPVMMGSGVFAGAFTLEHALAQRDGHGTSVAEALAAIGY
CCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
AGQAVSTPAVGAYFEAHIEQGPVLETNGCVIGVVRGALGQRWYDVTVQGMEAHAGPTPMD
CCCCCCCCCHHHHHHHHCCCCCEEECCCEEEEEEHHHHCCEEEEEEEEEEECCCCCCCHH
LRRDALLAAGSLVEEVNRIALAHAPHARGTVGVLEVFPSSRNVIPGSVRMTVDLRAADDA
HHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEEECCCE
VLLQMDAALREACERIGAARRTPVAVEQVVYFPPQPFTPELVEGVRADAAALGYSAMDVV
EEEEHHHHHHHHHHHHCCCCCCCCEEEHEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHH
SGAGHDAVYLARLAPTAMVFVPCADGISHNEIEDAEPEHLEAGCNVLLHAMLRSAGVAA
CCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9023955; 8285691 [H]