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The map label for this gene is yhcK [H]

Identifier: 120613358

GI number: 120613358

Start: 5274384

End: 5276102

Strand: Direct

Name: yhcK [H]

Synonym: Aave_4730

Alternate gene names: 120613358

Gene position: 5274384-5276102 (Clockwise)

Preceding gene: 120613356

Following gene: 120613365

Centisome position: 98.54

GC content: 74.46

Gene sequence:

>1719_bases
ATGTCCCTGAACCAATTCCTGCGCCTCATCGGCATCGTGCTGGCCACCGTGACGGCCCTGCTGGCCGCGCATGCCAGCTG
GGGGCAATGGCGCTCGTGGCGCGCGGCGGACGCCGGATCGCGCGCGCTCGCGGACCTCGGCCTGGGCATGGTGGCGACCG
AACGGGTGTCGCGCGAGCGCGGCCCGCTCGATGGAGCGCCCGATCCGCAGGCCGTCGCCGCCCTGGCGCGGGCGCGCGAA
AGCACCGACCAGGCCATGGCGCAGCTGCGCGCCATGCTCGCCGCCCGCACGCAGGTGCCGCACCTGGACGCACTGCTGGC
CGGCGCGGACGACGCCGCGCAGGCCCTCGCCCGTGCACGGGCGGTGGCCGACGGGGTCATCGCCCTCCCCCACGCCCAGC
GCACGCCCGAGGCCGTGCGCGCCAGCGTCTATGGACTGGCGGCCATCGTGCCGCGGCTCGCGCCGATCAACAGCGCACTG
ATCCAGACCGTTCGGACGGCACAGCCGTCCATGGACGGCGACACGCAGGTGCTGCGGATCACCGCGGAGCTGCGCGAGCA
TGCAGGCCTGCTCGGATCGCACTTCACCGCCGCGCTGGTGATGCAGCGTCCGTTCACGCCCGAAGAACGGCATGCGATCG
AGCAGACGCGCGGGCGCATCACGGCACTGCAGTTCCTGCTGGAGCAGCGCCTGGCCCGCCAGGATGCCCCGCCCGACATC
GCCCAGGCCTGGGAGGCCGTGGACAGCGGCTACTTCGGCCATGCGATGCGGGACATCGTCGCGCCCGTGCTGGCGGCCGG
CGAGCGGGACGGACGCTACGGCATCACCGCCGCCCAGTTCGCGGAGGGCTACGTGCCCGAACTGGGCAAGGTGGTGACAC
TGCGCGACCGGCTGATGGCGCGGCTGCAGGCCGACGCGCTCGCCGCCCGGGAGCGCGCCCTGCACAGCCTCATGGAGGTG
GCGGGCGCCTCGGCCCTGCTCTGGGCCATCCTCGCGGGCACGCTGGCCACGCTGCACGCCCGCGTGCTGCAGCCCCTGGC
CCAGACGACCCAGGCCCTGCAGGATCTGGCCCGCAACCGCCTGGACGCCCCGCTGCCCCGGCCCGCGGCCGACGACGAGA
TCGCCGCCGTGATCGGCGCCGTGCGTGCGCTGCAGGAGCACACGCAGGCCCGGCAGGTGCTCGAGCGCGAGCGCGACGGC
CTCATCGCGCAGCTGCGCGAGCAGTCGCTCACCGATTTCCTCACGGGGCTGCCCAATCGGCGCGCGTTTTTCGCGGCGGC
ACGCGAACGTATCGCGCAGGCACGGCAGCAGCGCTTCGACGTCGCCCTGATGCTGCTGGACGTGGACCTGTTCAAGAGCT
TCAACGACCGCGCGGGACACGCCGCCGGCGACGAGGCGCTGCGGGCCGTGGCACGCGCGGTGCGCCACACCCTGCGCCCG
GGCGACCTCGTGGCGCGGTACGGGGGCGAGGAGTTCGTGGTGCTGCTCGGCCCCTGCGCGGGCCGCGAGGGCGCGGCCTG
TGCCGAGCGCCTGCGCCACGCCATCGAGACGGCGCCGGTCGTGCTGCCGTCGGGGGAGCGCTTCCGGCTCACGGCCAGCG
TGGGCGTCGCCGTCTCCCAGCGCCACGGCATGGTGCTGGACCACCTGCTCTCGGAAGCGGACACGGCCCTCTACCGGGCC
AAGGACCGGGGCCGCAACCGGGTCGAGGAAGCCGCTTGA

Upstream 100 bases:

>100_bases
GGGACGACACCGTATGTAATAGAGTGCCGACAGTACATCACAATGCGAACAGTGCTCCGGCGGGCTGGCGGCCCCGGACC
GGTGGCGGCCACGTGCCTTC

Downstream 100 bases:

>100_bases
AAGACGGGAAAGACCCGCCGCGCCGGCCTGCGCCCCTCAGCGCTGCAGGGCGGCCGGAGGGCGCACGAAGTCGTCCAGCG
ACAGGCCCTTTTCCCGCAGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSLNQFLRLIGIVLATVTALLAAHASWGQWRSWRAADAGSRALADLGLGMVATERVSRERGPLDGAPDPQAVAALARARE
STDQAMAQLRAMLAARTQVPHLDALLAGADDAAQALARARAVADGVIALPHAQRTPEAVRASVYGLAAIVPRLAPINSAL
IQTVRTAQPSMDGDTQVLRITAELREHAGLLGSHFTAALVMQRPFTPEERHAIEQTRGRITALQFLLEQRLARQDAPPDI
AQAWEAVDSGYFGHAMRDIVAPVLAAGERDGRYGITAAQFAEGYVPELGKVVTLRDRLMARLQADALAARERALHSLMEV
AGASALLWAILAGTLATLHARVLQPLAQTTQALQDLARNRLDAPLPRPAADDEIAAVIGAVRALQEHTQARQVLERERDG
LIAQLREQSLTDFLTGLPNRRAFFAAARERIAQARQQRFDVALMLLDVDLFKSFNDRAGHAAGDEALRAVARAVRHTLRP
GDLVARYGGEEFVVLLGPCAGREGAACAERLRHAIETAPVVLPSGERFRLTASVGVAVSQRHGMVLDHLLSEADTALYRA
KDRGRNRVEEAA

Sequences:

>Translated_572_residues
MSLNQFLRLIGIVLATVTALLAAHASWGQWRSWRAADAGSRALADLGLGMVATERVSRERGPLDGAPDPQAVAALARARE
STDQAMAQLRAMLAARTQVPHLDALLAGADDAAQALARARAVADGVIALPHAQRTPEAVRASVYGLAAIVPRLAPINSAL
IQTVRTAQPSMDGDTQVLRITAELREHAGLLGSHFTAALVMQRPFTPEERHAIEQTRGRITALQFLLEQRLARQDAPPDI
AQAWEAVDSGYFGHAMRDIVAPVLAAGERDGRYGITAAQFAEGYVPELGKVVTLRDRLMARLQADALAARERALHSLMEV
AGASALLWAILAGTLATLHARVLQPLAQTTQALQDLARNRLDAPLPRPAADDEIAAVIGAVRALQEHTQARQVLERERDG
LIAQLREQSLTDFLTGLPNRRAFFAAARERIAQARQQRFDVALMLLDVDLFKSFNDRAGHAAGDEALRAVARAVRHTLRP
GDLVARYGGEEFVVLLGPCAGREGAACAERLRHAIETAPVVLPSGERFRLTASVGVAVSQRHGMVLDHLLSEADTALYRA
KDRGRNRVEEAA
>Mature_571_residues
SLNQFLRLIGIVLATVTALLAAHASWGQWRSWRAADAGSRALADLGLGMVATERVSRERGPLDGAPDPQAVAALARARES
TDQAMAQLRAMLAARTQVPHLDALLAGADDAAQALARARAVADGVIALPHAQRTPEAVRASVYGLAAIVPRLAPINSALI
QTVRTAQPSMDGDTQVLRITAELREHAGLLGSHFTAALVMQRPFTPEERHAIEQTRGRITALQFLLEQRLARQDAPPDIA
QAWEAVDSGYFGHAMRDIVAPVLAAGERDGRYGITAAQFAEGYVPELGKVVTLRDRLMARLQADALAARERALHSLMEVA
GASALLWAILAGTLATLHARVLQPLAQTTQALQDLARNRLDAPLPRPAADDEIAAVIGAVRALQEHTQARQVLERERDGL
IAQLREQSLTDFLTGLPNRRAFFAAARERIAQARQQRFDVALMLLDVDLFKSFNDRAGHAAGDEALRAVARAVRHTLRPG
DLVARYGGEEFVVLLGPCAGREGAACAERLRHAIETAPVVLPSGERFRLTASVGVAVSQRHGMVLDHLLSEADTALYRAK
DRGRNRVEEAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=40.5063291139241, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=41.2790697674419, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=36.6863905325444, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=39.766081871345, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=35.625, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=35.2601156069364, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788381, Length=191, Percent_Identity=34.0314136125654, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787816, Length=171, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=31.2883435582822, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081977, Length=172, Percent_Identity=31.9767441860465, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=36.094674556213, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081974, Length=111, Percent_Identity=29.7297297297297, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788956, Length=240, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 61685; Mature: 61554

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNQFLRLIGIVLATVTALLAAHASWGQWRSWRAADAGSRALADLGLGMVATERVSRER
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHC
GPLDGAPDPQAVAALARARESTDQAMAQLRAMLAARTQVPHLDALLAGADDAAQALARAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHH
AVADGVIALPHAQRTPEAVRASVYGLAAIVPRLAPINSALIQTVRTAQPSMDGDTQVLRI
HHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHH
TAELREHAGLLGSHFTAALVMQRPFTPEERHAIEQTRGRITALQFLLEQRLARQDAPPDI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AQAWEAVDSGYFGHAMRDIVAPVLAAGERDGRYGITAAQFAEGYVPELGKVVTLRDRLMA
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
RLQADALAARERALHSLMEVAGASALLWAILAGTLATLHARVLQPLAQTTQALQDLARNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDAPLPRPAADDEIAAVIGAVRALQEHTQARQVLERERDGLIAQLREQSLTDFLTGLPNR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
RAFFAAARERIAQARQQRFDVALMLLDVDLFKSFNDRAGHAAGDEALRAVARAVRHTLRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDLVARYGGEEFVVLLGPCAGREGAACAERLRHAIETAPVVLPSGERFRLTASVGVAVSQ
CHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEEHHHHHHHH
RHGMVLDHLLSEADTALYRAKDRGRNRVEEAA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLNQFLRLIGIVLATVTALLAAHASWGQWRSWRAADAGSRALADLGLGMVATERVSRER
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHHHC
GPLDGAPDPQAVAALARARESTDQAMAQLRAMLAARTQVPHLDALLAGADDAAQALARAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHH
AVADGVIALPHAQRTPEAVRASVYGLAAIVPRLAPINSALIQTVRTAQPSMDGDTQVLRI
HHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHH
TAELREHAGLLGSHFTAALVMQRPFTPEERHAIEQTRGRITALQFLLEQRLARQDAPPDI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AQAWEAVDSGYFGHAMRDIVAPVLAAGERDGRYGITAAQFAEGYVPELGKVVTLRDRLMA
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
RLQADALAARERALHSLMEVAGASALLWAILAGTLATLHARVLQPLAQTTQALQDLARNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDAPLPRPAADDEIAAVIGAVRALQEHTQARQVLERERDGLIAQLREQSLTDFLTGLPNR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCC
RAFFAAARERIAQARQQRFDVALMLLDVDLFKSFNDRAGHAAGDEALRAVARAVRHTLRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
GDLVARYGGEEFVVLLGPCAGREGAACAERLRHAIETAPVVLPSGERFRLTASVGVAVSQ
CHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEEHHHHHHHH
RHGMVLDHLLSEADTALYRAKDRGRNRVEEAA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]