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The map label for this gene is mdeA [H]

Identifier: 120611749

GI number: 120611749

Start: 3407146

End: 3408432

Strand: Direct

Name: mdeA [H]

Synonym: Aave_3089

Alternate gene names: 120611749

Gene position: 3407146-3408432 (Clockwise)

Preceding gene: 120611747

Following gene: 120611752

Centisome position: 63.65

GC content: 69.7

Gene sequence:

>1287_bases
ATGAAGATCGAGACCCTCGCCGTCCACGCCGGACAGGCACCCGACCCCACCACCCGCGCCGTGGCGGTGCCCATCTACCA
GACGGCGGCCTACGCCTTCGACAGCGCCCGGCACGGCGCGGACCTGTTCGACCTGAAGGTGCCGGGCAACATCTACACCC
GCATCATGAATCCCACCAACGACGTGCTCGAAAAGCGCGTCGCGGCGCTCGAAGGCGGCATCGCCGCGCTGGCGCTGGCC
TCGGGCATGGCGGCCATCACCTACGCGATCCAGACGATCGCCGAGGCCGGCGACAACATCGTCTCGGCCAGCACGCTCTA
TGGCGGCACCTACAACCTGTTCGCGCACACGCTGCCGCGCCAGGGCATCATCACCCGCTTCGCGGACCCGCGCGACCCGG
CCAGCTTCGCGCGGCACATCGACGGCCGCACCAAGGCCGTGTTCGTCGAATCCATCGGCAACCCGCTGGGCAACGTGACC
GACATCGCCGCGCTGGCACGGGTGGCCCATGACCACGGCGTGCCGCTGATCGTGGACAACACCGTGCCCAGCCCCTACCT
GCTGCGGCCCATCGAGCACGGAGCGGACATCGTCGTCCACTCGCTCACCAAGTACCTGGGCGGCCATGGCAACAGCATCG
GCGGCGCCATCGTGGACAGCGGCAAATTCCCTTGGGGCGAGCACAAGGAGCGTTTCGCCTGCCTCAACACCCCCGACCCC
AGCTACCACGGCGTGGTGTACACCGAAGCCCTGGGCGAGGCGGCCTTCATCGGCCGCGCGCGCGTCGTCCCGCTGCGCAA
CACCGGCGCCACGCTCTCCCCGCACAACGCCTTCCTGATCCTGCAGGGCATCGAAACCCTGGCCCTGCGCATGGACCGCA
TCTGCGACAACACCCTGGCTGTCGCGCAGTACCTGCAGCAGCACCCCAAGGTGGCACAGGTGCGTTATGCCGCCCTGCCC
GGCCACCCCGACCATGCGATCACGCAGCGCCTGCTCGGCGGCAGGGCCTCCGGCATCCTCTCCTTCGAGCTGCGGTCCTC
GCAGGGCGAGGATCCCCGGGCGGCCGGCGCGCGCTTCCTCGATGCCTTGCAGCTCTTCACGCGGCTCGTGAACATCGGCG
ACGCGCGCTCGCTGGCCACGCACCCCGCTTCCACCACCCACCGCCAGTTGGGGCCGGAAGAACTGCAGCAGGCCGGCGTG
GCCGAGGGCATGGTGCGCCTGTCGGTGGGCATCGAGCACATCGACGACCTCACCGCCGACCTCGCCCAGGCACTGGACGC
CGTGTGA

Upstream 100 bases:

>100_bases
TAGCGATCGGTTCGTTCCGCGTGGCGGTGCGACCCGACACAATGGGCCGCACGCACCGGGCGATGCCGGCGCGCCACTGC
TTCCGAGGAGACAGCCCAGC

Downstream 100 bases:

>100_bases
CGCGATGCCGCGCGGATCAGGCGCTCTCCAGGTCGCGCGCGAGGCGCAGGCCGGAGAACTGCCACCGCGCGGTGGCCGGG
AAGAAATTCCGGTAGCTGGC

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKIETLAVHAGQAPDPTTRAVAVPIYQTAAYAFDSARHGADLFDLKVPGNIYTRIMNPTNDVLEKRVAALEGGIAALALA
SGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPRQGIITRFADPRDPASFARHIDGRTKAVFVESIGNPLGNVT
DIAALARVAHDHGVPLIVDNTVPSPYLLRPIEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGEHKERFACLNTPDP
SYHGVVYTEALGEAAFIGRARVVPLRNTGATLSPHNAFLILQGIETLALRMDRICDNTLAVAQYLQQHPKVAQVRYAALP
GHPDHAITQRLLGGRASGILSFELRSSQGEDPRAAGARFLDALQLFTRLVNIGDARSLATHPASTTHRQLGPEELQQAGV
AEGMVRLSVGIEHIDDLTADLAQALDAV

Sequences:

>Translated_428_residues
MKIETLAVHAGQAPDPTTRAVAVPIYQTAAYAFDSARHGADLFDLKVPGNIYTRIMNPTNDVLEKRVAALEGGIAALALA
SGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPRQGIITRFADPRDPASFARHIDGRTKAVFVESIGNPLGNVT
DIAALARVAHDHGVPLIVDNTVPSPYLLRPIEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGEHKERFACLNTPDP
SYHGVVYTEALGEAAFIGRARVVPLRNTGATLSPHNAFLILQGIETLALRMDRICDNTLAVAQYLQQHPKVAQVRYAALP
GHPDHAITQRLLGGRASGILSFELRSSQGEDPRAAGARFLDALQLFTRLVNIGDARSLATHPASTTHRQLGPEELQQAGV
AEGMVRLSVGIEHIDDLTADLAQALDAV
>Mature_428_residues
MKIETLAVHAGQAPDPTTRAVAVPIYQTAAYAFDSARHGADLFDLKVPGNIYTRIMNPTNDVLEKRVAALEGGIAALALA
SGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPRQGIITRFADPRDPASFARHIDGRTKAVFVESIGNPLGNVT
DIAALARVAHDHGVPLIVDNTVPSPYLLRPIEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGEHKERFACLNTPDP
SYHGVVYTEALGEAAFIGRARVVPLRNTGATLSPHNAFLILQGIETLALRMDRICDNTLAVAQYLQQHPKVAQVRYAALP
GHPDHAITQRLLGGRASGILSFELRSSQGEDPRAAGARFLDALQLFTRLVNIGDARSLATHPASTTHRQLGPEELQQAGV
AEGMVRLSVGIEHIDDLTADLAQALDAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=430, Percent_Identity=34.6511627906977, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI299473759, Length=430, Percent_Identity=31.3953488372093, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI25453487, Length=429, Percent_Identity=30.7692307692308, Blast_Score=143, Evalue=4e-34,
Organism=Escherichia coli, GI1790375, Length=426, Percent_Identity=33.0985915492958, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=27.5294117647059, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=30.622009569378, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17538055, Length=426, Percent_Identity=35.6807511737089, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=430, Percent_Identity=34.8837209302326, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=47.0046082949309, Blast_Score=368, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319307, Length=434, Percent_Identity=31.3364055299539, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6321254, Length=446, Percent_Identity=26.2331838565022, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=336, Percent_Identity=27.6785714285714, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=435, Percent_Identity=24.5977011494253, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922554, Length=428, Percent_Identity=34.5794392523364, Blast_Score=212, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45598; Mature: 45598

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIETLAVHAGQAPDPTTRAVAVPIYQTAAYAFDSARHGADLFDLKVPGNIYTRIMNPTN
CCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHEEEECCCHH
DVLEKRVAALEGGIAALALASGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCHHHHHHHHCCC
QGIITRFADPRDPASFARHIDGRTKAVFVESIGNPLGNVTDIAALARVAHDHGVPLIVDN
CCCHHHCCCCCCHHHHHHHCCCCCCEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECC
TVPSPYLLRPIEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGEHKERFACLNTPDP
CCCCCCEECCHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCC
SYHGVVYTEALGEAAFIGRARVVPLRNTGATLSPHNAFLILQGIETLALRMDRICDNTLA
CCCCEEEHHHHCCHHHCCCEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
VAQYLQQHPKVAQVRYAALPGHPDHAITQRLLGGRASGILSFELRSSQGEDPRAAGARFL
HHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
DALQLFTRLVNIGDARSLATHPASTTHRQLGPEELQQAGVAEGMVRLSVGIEHIDDLTAD
HHHHHHHHHHCCCCCHHHCCCCCCCHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LAQALDAV
HHHHHHCC
>Mature Secondary Structure
MKIETLAVHAGQAPDPTTRAVAVPIYQTAAYAFDSARHGADLFDLKVPGNIYTRIMNPTN
CCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHEEEECCCHH
DVLEKRVAALEGGIAALALASGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHCCCCHHHHHHHHCCC
QGIITRFADPRDPASFARHIDGRTKAVFVESIGNPLGNVTDIAALARVAHDHGVPLIVDN
CCCHHHCCCCCCHHHHHHHCCCCCCEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCEEECC
TVPSPYLLRPIEHGADIVVHSLTKYLGGHGNSIGGAIVDSGKFPWGEHKERFACLNTPDP
CCCCCCEECCHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCC
SYHGVVYTEALGEAAFIGRARVVPLRNTGATLSPHNAFLILQGIETLALRMDRICDNTLA
CCCCEEEHHHHCCHHHCCCEEEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHH
VAQYLQQHPKVAQVRYAALPGHPDHAITQRLLGGRASGILSFELRSSQGEDPRAAGARFL
HHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
DALQLFTRLVNIGDARSLATHPASTTHRQLGPEELQQAGVAEGMVRLSVGIEHIDDLTAD
HHHHHHHHHHCCCCCHHHCCCCCCCHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LAQALDAV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]