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The map label for this gene is pleD [H]

Identifier: 120611687

GI number: 120611687

Start: 3333607

End: 3334389

Strand: Direct

Name: pleD [H]

Synonym: Aave_3024

Alternate gene names: 120611687

Gene position: 3333607-3334389 (Clockwise)

Preceding gene: 120611686

Following gene: 120611688

Centisome position: 62.28

GC content: 67.82

Gene sequence:

>783_bases
TTGTTGCATTTTGTGTCTGACTCCGCGACCCCCCCACTTGTGGACCTGCGCGACCTGCGCCTGACGACGGCGCACGCCCT
GGTCGCGCAGGCGCTGGGCTCCGAAGCCGCCATCCCCAGGGAATCGCCCCGGCATTACCTGCAGGGCCTGATCGACGGCC
TCTGCGAGCTTTCGTTGAAGGACCCGCTCACGGGCCTGGCGAACCGGCGGCATTTCCGGACCGTGCTCGAGCGCGAGATC
GACCGCGTCACGCGCGCGGGCGAGGCTGCGCTGCTGCTGATGCTCGACATCGACAACTTCAAGCGGGTCAACGACACCCA
CGGCCACATGGCCGGCGACATCGTCCTGCAGTCGATCGCGCGCACCCTGAGCCGCTGCGTCCGGCCCATGGACACCCTGT
CGCGCTACGGCGGCGAGGAATTCGCCGTGGTCCTGCCCGCCTGCCAGGCCGGTTTCGGCAAGGTGGTGGCAGAGCGCATC
CGCCGCGCGGTGGAGCGCACCCCCGTCCGCATCTCGCCGACGGTCGAACTCAACGTCACCGTCAGCATCGGCGGCGCGTT
CGCGCTCCAGTGGATCCGCAGCACCACGCTGCTGTGGACGGAGCGCGCGGACAGCCAGCTCTACAAGGCCAAGCTGGCTG
GACGCAACCGGGTCAGCATCGAAGAACAGCCGGACAGCACGGTGAGCGCCGAAGAGAAGAGTTTGCTTTTCGGCCCGCTG
TACACTCCCTCGGGTTGGGGCGACGTTCCCCCTCCCTTGGATTCCACAGGCAGCGCCTATTGA

Upstream 100 bases:

>100_bases
TGCTCTACCGCACCGACGCCGAAGCCCTGCTGGCATTGCAACGTTTACCTTTGTAGTTACCTATGGCGGGGCAGCCGCTG
ATACACTTCACGGACATTGT

Downstream 100 bases:

>100_bases
AAGCAAAGAGATGACCGACGCGCTGTCACCCCGCACCACGTCCGCTGCGCCCCAGGCGCCAGCCGGTGCCCGCATCATTG
CCATCACCAGCGGCAAGGGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLHFVSDSATPPLVDLRDLRLTTAHALVAQALGSEAAIPRESPRHYLQGLIDGLCELSLKDPLTGLANRRHFRTVLEREI
DRVTRAGEAALLLMLDIDNFKRVNDTHGHMAGDIVLQSIARTLSRCVRPMDTLSRYGGEEFAVVLPACQAGFGKVVAERI
RRAVERTPVRISPTVELNVTVSIGGAFALQWIRSTTLLWTERADSQLYKAKLAGRNRVSIEEQPDSTVSAEEKSLLFGPL
YTPSGWGDVPPPLDSTGSAY

Sequences:

>Translated_260_residues
MLHFVSDSATPPLVDLRDLRLTTAHALVAQALGSEAAIPRESPRHYLQGLIDGLCELSLKDPLTGLANRRHFRTVLEREI
DRVTRAGEAALLLMLDIDNFKRVNDTHGHMAGDIVLQSIARTLSRCVRPMDTLSRYGGEEFAVVLPACQAGFGKVVAERI
RRAVERTPVRISPTVELNVTVSIGGAFALQWIRSTTLLWTERADSQLYKAKLAGRNRVSIEEQPDSTVSAEEKSLLFGPL
YTPSGWGDVPPPLDSTGSAY
>Mature_260_residues
MLHFVSDSATPPLVDLRDLRLTTAHALVAQALGSEAAIPRESPRHYLQGLIDGLCELSLKDPLTGLANRRHFRTVLEREI
DRVTRAGEAALLLMLDIDNFKRVNDTHGHMAGDIVLQSIARTLSRCVRPMDTLSRYGGEEFAVVLPACQAGFGKVVAERI
RRAVERTPVRISPTVELNVTVSIGGAFALQWIRSTTLLWTERADSQLYKAKLAGRNRVSIEEQPDSTVSAEEKSLLFGPL
YTPSGWGDVPPPLDSTGSAY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=37.1951219512195, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=38.125, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=35.9281437125749, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87081881, Length=159, Percent_Identity=40.8805031446541, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=35.7142857142857, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787816, Length=172, Percent_Identity=37.7906976744186, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787802, Length=136, Percent_Identity=37.5, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082007, Length=134, Percent_Identity=36.5671641791045, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=30.8988764044944, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=35.7142857142857, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=27.3291925465839, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787541, Length=164, Percent_Identity=29.8780487804878, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 28554; Mature: 28554

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHFVSDSATPPLVDLRDLRLTTAHALVAQALGSEAAIPRESPRHYLQGLIDGLCELSLK
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DPLTGLANRRHFRTVLEREIDRVTRAGEAALLLMLDIDNFKRVNDTHGHMAGDIVLQSIA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHCCCCCCHHHHHHHHHHHH
RTLSRCVRPMDTLSRYGGEEFAVVLPACQAGFGKVVAERIRRAVERTPVRISPTVELNVT
HHHHHHHCHHHHHHHCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHCCEEECCEEEEEEE
VSIGGAFALQWIRSTTLLWTERADSQLYKAKLAGRNRVSIEEQPDSTVSAEEKSLLFGPL
EEECHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECC
YTPSGWGDVPPPLDSTGSAY
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLHFVSDSATPPLVDLRDLRLTTAHALVAQALGSEAAIPRESPRHYLQGLIDGLCELSLK
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCC
DPLTGLANRRHFRTVLEREIDRVTRAGEAALLLMLDIDNFKRVNDTHGHMAGDIVLQSIA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHCCCCCCHHHHHHHHHHHH
RTLSRCVRPMDTLSRYGGEEFAVVLPACQAGFGKVVAERIRRAVERTPVRISPTVELNVT
HHHHHHHCHHHHHHHCCCCCEEEEECHHHCCHHHHHHHHHHHHHHHCCEEECCEEEEEEE
VSIGGAFALQWIRSTTLLWTERADSQLYKAKLAGRNRVSIEEQPDSTVSAEEKSLLFGPL
EEECHHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECC
YTPSGWGDVPPPLDSTGSAY
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA