Click here to switch to the map view.

The map label for this gene is ycdT [H]

Identifier: 120611590

GI number: 120611590

Start: 3216518

End: 3217270

Strand: Reverse

Name: ycdT [H]

Synonym: Aave_2926

Alternate gene names: 120611590

Gene position: 3217270-3216518 (Counterclockwise)

Preceding gene: 120611591

Following gene: 120611589

Centisome position: 60.1

GC content: 69.72

Gene sequence:

>753_bases
ATGGACGCCATGGCCACGCCACCCGATCTCTTCGAATCCGAGAATCGCGCACTGCTGTCCGCCCGCGCCGCTTATGAGGC
ATGCCATACACCCGGGCACGGCGAGGGATGCCGGCAGGCGCTGGGGGACCTGCTCGCCGCCTATGAGCGGCTCCTGCGCG
AAACACGGCGCCTCGTGCGCCGCAGCGACCGGGCCGAACTGGAGATGAACCAGCTCAACCAGCAGCTGACGGAGCTGGCG
GAGCAATTGCACTACCGCGCCACCCACGATCCGCTGACCGGCGTGCTCAACCGGGCGGCCATCATCGAGCAGGTCAACGG
CCGGTTCGAGACCGGCAGCGTCGCGCTCATCGTGCTGGACATCGACCATTTCAAGAAGGTGAACGACAGCTTCGGCCATC
CCGTCGGCGACAGCGTCATCCGCGGCGTGGTGGATCGGCTCAAGTCCGTGGTGCCCGGTTCGGCGCAGATCGGCCGGGTC
GGCGGGGAGGAGTTCACGGTGCTGCTGCCCGAGGAGGACGCGATGGCCTGCGAACGCGTGGCCGAGGCCGCCCGCCGGGC
CATCGAGGGCTTCGATTTCGGCCTGCCCGACGGCAGCCGCGTGACCGCCAGCTTCGGAGTGAGCTGGCTGCCGGGCGGCT
GTGTGTTCGACGATGCCTACGGGCTGGCCGACGAGGCGCTCTACGGTGCCAAGCGCGCGGGTCGCAATCGCGTGGTGCGC
GCGGCCCCGGCGGCGCACGCGCCGAATCCATGA

Upstream 100 bases:

>100_bases
CGATGAGGAGGACGACACCATCCTCGAATTCGGCCAGGAATTGAGCGACGATTTTCCCGAGCTCGAATTCACCTGCCACC
CGGTAATGCCGTCCTGACCG

Downstream 100 bases:

>100_bases
GCATCCAGGTCGGGATGGACGGCCCCGTCGCCGAGCCCGAATGCCAGCCCGCCGCCGCGCCGTGCGCGGACCCGGCGTGG
CAGGTACTGGTGGTGGACGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MDAMATPPDLFESENRALLSARAAYEACHTPGHGEGCRQALGDLLAAYERLLRETRRLVRRSDRAELEMNQLNQQLTELA
EQLHYRATHDPLTGVLNRAAIIEQVNGRFETGSVALIVLDIDHFKKVNDSFGHPVGDSVIRGVVDRLKSVVPGSAQIGRV
GGEEFTVLLPEEDAMACERVAEAARRAIEGFDFGLPDGSRVTASFGVSWLPGGCVFDDAYGLADEALYGAKRAGRNRVVR
AAPAAHAPNP

Sequences:

>Translated_250_residues
MDAMATPPDLFESENRALLSARAAYEACHTPGHGEGCRQALGDLLAAYERLLRETRRLVRRSDRAELEMNQLNQQLTELA
EQLHYRATHDPLTGVLNRAAIIEQVNGRFETGSVALIVLDIDHFKKVNDSFGHPVGDSVIRGVVDRLKSVVPGSAQIGRV
GGEEFTVLLPEEDAMACERVAEAARRAIEGFDFGLPDGSRVTASFGVSWLPGGCVFDDAYGLADEALYGAKRAGRNRVVR
AAPAAHAPNP
>Mature_250_residues
MDAMATPPDLFESENRALLSARAAYEACHTPGHGEGCRQALGDLLAAYERLLRETRRLVRRSDRAELEMNQLNQQLTELA
EQLHYRATHDPLTGVLNRAAIIEQVNGRFETGSVALIVLDIDHFKKVNDSFGHPVGDSVIRGVVDRLKSVVPGSAQIGRV
GGEEFTVLLPEEDAMACERVAEAARRAIEGFDFGLPDGSRVTASFGVSWLPGGCVFDDAYGLADEALYGAKRAGRNRVVR
AAPAAHAPNP

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=39.8773006134969, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87082007, Length=208, Percent_Identity=33.1730769230769, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693134, Length=212, Percent_Identity=31.1320754716981, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=36.6863905325444, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=38.121546961326, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788381, Length=194, Percent_Identity=33.5051546391753, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787802, Length=170, Percent_Identity=31.7647058823529, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87081977, Length=183, Percent_Identity=31.1475409836066, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788956, Length=179, Percent_Identity=30.7262569832402, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=32.7160493827161, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=32.1428571428571, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=29.5180722891566, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=30.379746835443, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=25.4901960784314, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 27103; Mature: 27103

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAMATPPDLFESENRALLSARAAYEACHTPGHGEGCRQALGDLLAAYERLLRETRRLVR
CCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RSDRAELEMNQLNQQLTELAEQLHYRATHDPLTGVLNRAAIIEQVNGRFETGSVALIVLD
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
IDHFKKVNDSFGHPVGDSVIRGVVDRLKSVVPGSAQIGRVGGEEFTVLLPEEDAMACERV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
AEAARRAIEGFDFGLPDGSRVTASFGVSWLPGGCVFDDAYGLADEALYGAKRAGRNRVVR
HHHHHHHHHHCCCCCCCCCEEEHHCCCCCCCCCCCCCCHHCHHHHHHHHHHHHCCCCEEE
AAPAAHAPNP
ECCCCCCCCC
>Mature Secondary Structure
MDAMATPPDLFESENRALLSARAAYEACHTPGHGEGCRQALGDLLAAYERLLRETRRLVR
CCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RSDRAELEMNQLNQQLTELAEQLHYRATHDPLTGVLNRAAIIEQVNGRFETGSVALIVLD
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
IDHFKKVNDSFGHPVGDSVIRGVVDRLKSVVPGSAQIGRVGGEEFTVLLPEEDAMACERV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
AEAARRAIEGFDFGLPDGSRVTASFGVSWLPGGCVFDDAYGLADEALYGAKRAGRNRVVR
HHHHHHHHHHCCCCCCCCCEEEHHCCCCCCCCCCCCCCHHCHHHHHHHHHHHHCCCCEEE
AAPAAHAPNP
ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]