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The map label for this gene is eda [H]

Identifier: 120611456

GI number: 120611456

Start: 3064982

End: 3065635

Strand: Reverse

Name: eda [H]

Synonym: Aave_2792

Alternate gene names: 120611456

Gene position: 3065635-3064982 (Counterclockwise)

Preceding gene: 120611457

Following gene: 120611453

Centisome position: 57.27

GC content: 73.09

Gene sequence:

>654_bases
ATGGCTGTGAACGAACGCAATGCTTTGACCGCGCAGGACGTGATGCGCGACGCGCCGGTGATCCCGGTCATCGTGCTGAC
CGACGTGGCGCACGCCGTGCCCATGGCGCGCGCTCTGGTGGCCGGCGGCATCCGCATGCTGGAAGTGACGCTGCGCACGC
CCCAGGCCCTGGCCTGCATGGAAGCCATCGCCAAGGACGTGCCCGAGGCCGTGGTCGGCGCCGGTACCGTGCGCAGCGCG
GCCGATGCGCAGGCCGCCGCCCGGGCGGGCGCGCGTTTCGCCGTGAGCCCGGGCTTCACGCCCGCCGTGGGCCGCGCCTG
CCGCGATCTCGGCCTGCCGCTGCTGCCCGGCGTGGCCACCGGCAGCGAGATCATGATGGCGCAGGAAGAGGGCTTCAACG
CCCTGAAGTTCTTCCCCGCCGTTCAGGCCGGCGGCCTCGCCATGCTCAAGGCGTGGCAGGGCCCCTTCGGCGACGTGGTG
TTCTGCCCCACGGGCGGCATCCAGCCGGGCAATGCGGCGGATTTCCTGGCGCTGGGCAACGTGGCCTGCGTGGGCGGCTC
GTGGCTGGTGCCCGGCGACGCGCTGGCATCTGGCGACTGGGCGCGCATCACCGAACTGGCCCGGGCCGCCACGGCATTGC
GTCCGGCACGCTGA

Upstream 100 bases:

>100_bases
AGCCCGAGGAACTGGCCCGTGCCGACGGCCACGGCTGGGGCCGCGAACTGTTTGCCGGATTCCGGCGCAATGCGCTCAGC
GCGGAAGAGGGAGCCTGCAC

Downstream 100 bases:

>100_bases
CGGCAGGAAAGGAAATATCGCCAACCTGCGGGGTTGGCGATACTGGCGCCATGAAAACCATCGACGAGATGCTGAACCTC
GAGCTGCTTACCCCCGAGCA

Product: 2-keto-3-deoxy-phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MAVNERNALTAQDVMRDAPVIPVIVLTDVAHAVPMARALVAGGIRMLEVTLRTPQALACMEAIAKDVPEAVVGAGTVRSA
ADAQAAARAGARFAVSPGFTPAVGRACRDLGLPLLPGVATGSEIMMAQEEGFNALKFFPAVQAGGLAMLKAWQGPFGDVV
FCPTGGIQPGNAADFLALGNVACVGGSWLVPGDALASGDWARITELARAATALRPAR

Sequences:

>Translated_217_residues
MAVNERNALTAQDVMRDAPVIPVIVLTDVAHAVPMARALVAGGIRMLEVTLRTPQALACMEAIAKDVPEAVVGAGTVRSA
ADAQAAARAGARFAVSPGFTPAVGRACRDLGLPLLPGVATGSEIMMAQEEGFNALKFFPAVQAGGLAMLKAWQGPFGDVV
FCPTGGIQPGNAADFLALGNVACVGGSWLVPGDALASGDWARITELARAATALRPAR
>Mature_216_residues
AVNERNALTAQDVMRDAPVIPVIVLTDVAHAVPMARALVAGGIRMLEVTLRTPQALACMEAIAKDVPEAVVGAGTVRSAA
DAQAAARAGARFAVSPGFTPAVGRACRDLGLPLLPGVATGSEIMMAQEEGFNALKFFPAVQAGGLAMLKAWQGPFGDVVF
CPTGGIQPGNAADFLALGNVACVGGSWLVPGDALASGDWARITELARAATALRPAR

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=182, Percent_Identity=55.4945054945055, Blast_Score=203, Evalue=8e-54,
Organism=Escherichia coli, GI48994954, Length=144, Percent_Identity=32.6388888888889, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22113; Mature: 21982

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNERNALTAQDVMRDAPVIPVIVLTDVAHAVPMARALVAGGIRMLEVTLRTPQALACM
CCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHH
EAIAKDVPEAVVGAGTVRSAADAQAAARAGARFAVSPGFTPAVGRACRDLGLPLLPGVAT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCC
GSEIMMAQEEGFNALKFFPAVQAGGLAMLKAWQGPFGDVVFCPTGGIQPGNAADFLALGN
CCCEEEEHHCCCCHHHHCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCC
VACVGGSWLVPGDALASGDWARITELARAATALRPAR
EEEECCCEECCCHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVNERNALTAQDVMRDAPVIPVIVLTDVAHAVPMARALVAGGIRMLEVTLRTPQALACM
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHH
EAIAKDVPEAVVGAGTVRSAADAQAAARAGARFAVSPGFTPAVGRACRDLGLPLLPGVAT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCC
GSEIMMAQEEGFNALKFFPAVQAGGLAMLKAWQGPFGDVVFCPTGGIQPGNAADFLALGN
CCCEEEEHHCCCCHHHHCCCCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCC
VACVGGSWLVPGDALASGDWARITELARAATALRPAR
EEEECCCEECCCHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]