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The map label for this gene is paaF [C]

Identifier: 120610199

GI number: 120610199

Start: 1678974

End: 1679768

Strand: Direct

Name: paaF [C]

Synonym: Aave_1514

Alternate gene names: 120610199

Gene position: 1678974-1679768 (Clockwise)

Preceding gene: 120610198

Following gene: 120610200

Centisome position: 31.37

GC content: 73.33

Gene sequence:

>795_bases
ATGGCGGGTGGTGAGGTCGGGGTGGTCTGGCCGCAAGGGGGTGCGCAGGAAGGGCGTGGCATCGTGCGTGTCACGTTGCG
GCATCCGGGGCGGCTCAATGCCATGTCGCGGGCGATGTGGCGGCAGTTGCGGGAGGTGTTCGAGGGCATCCAGGCCCGCG
AGGACGCGCGCTGCGTGCTCATCGAGGGGGAGGGCGATGCCTTCTGCGCGGGGGGCGATATCTCCGAATACCCGGCGTTC
CGGTTCGATCCCGGGAGCCTGCGCGACTTCCACGAGCACGATGTGTGGGGCGGGCTGTCGGCGATGCTGGCGTGCGACGT
GCCGGTCGTCGCCGTGATCCGTGGTGCCTGCATGGGCGCCGGGATGGAGATCGCGGCCTGCTGCGACGTGCGGTTCGCGG
CGGTATCCGCGCGCTTCGGCGCACCGATCGCGCGGCTGGGGTTCCCGATGGCGCCGCGCGAGGCCGCGCTGGTGGCGCAG
GCCGTGGGCGATGGCCTGGCCCGCCGCATGCTGCTGGAGGCCGCCACGTTCGGCGCGGGGCCGCTGGCCGCGCAGGGTTT
CCTGGCGGCCGTGGTGGCGGACGACGGACTGGCCGCGCAGGCGTGGGCGAGCGCGGAGCGCATCGCGGCGCTGGCACCCG
AGGCGGCGCGGCTCAACAAGCAGACGCTGCGCGCGTGCCGGCAGGCTGCCGGCGGCGCTCCGCAGGCCGTTTCCGATCCG
TATGCCTACGCGGCCGGCGCGGAGCACCGCGAAGGCATCTCCGCTTTCCTGGAAAAGCGGACACCTCGGTTCTGA

Upstream 100 bases:

>100_bases
GCGGCCTCTCGATCGGGTGGCATTGCGTGGATTCCGGCATTTGTCGGCGTGGCGATGGTCGGGTGAGCAGATGAACTTTT
GTTGAACAGGGGGCCGTGGA

Downstream 100 bases:

>100_bases
GTCACGCATTCCTTCATCTTCTTCCGGCATCCATCCGAGTTCGCCGTTTCCCAGTTTTCATTCGTCCATCCATTTGCTCA
TCACCATGTCTTCCCAACCG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAGGEVGVVWPQGGAQEGRGIVRVTLRHPGRLNAMSRAMWRQLREVFEGIQAREDARCVLIEGEGDAFCAGGDISEYPAF
RFDPGSLRDFHEHDVWGGLSAMLACDVPVVAVIRGACMGAGMEIAACCDVRFAAVSARFGAPIARLGFPMAPREAALVAQ
AVGDGLARRMLLEAATFGAGPLAAQGFLAAVVADDGLAAQAWASAERIAALAPEAARLNKQTLRACRQAAGGAPQAVSDP
YAYAAGAEHREGISAFLEKRTPRF

Sequences:

>Translated_264_residues
MAGGEVGVVWPQGGAQEGRGIVRVTLRHPGRLNAMSRAMWRQLREVFEGIQAREDARCVLIEGEGDAFCAGGDISEYPAF
RFDPGSLRDFHEHDVWGGLSAMLACDVPVVAVIRGACMGAGMEIAACCDVRFAAVSARFGAPIARLGFPMAPREAALVAQ
AVGDGLARRMLLEAATFGAGPLAAQGFLAAVVADDGLAAQAWASAERIAALAPEAARLNKQTLRACRQAAGGAPQAVSDP
YAYAAGAEHREGISAFLEKRTPRF
>Mature_263_residues
AGGEVGVVWPQGGAQEGRGIVRVTLRHPGRLNAMSRAMWRQLREVFEGIQAREDARCVLIEGEGDAFCAGGDISEYPAFR
FDPGSLRDFHEHDVWGGLSAMLACDVPVVAVIRGACMGAGMEIAACCDVRFAAVSARFGAPIARLGFPMAPREAALVAQA
VGDGLARRMLLEAATFGAGPLAAQGFLAAVVADDGLAAQAWASAERIAALAPEAARLNKQTLRACRQAAGGAPQAVSDPY
AYAAGAEHREGISAFLEKRTPRF

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=29.1828793774319, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194097323, Length=259, Percent_Identity=24.7104247104247, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=209, Percent_Identity=22.0095693779904, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260275230, Length=209, Percent_Identity=22.0095693779904, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260274832, Length=209, Percent_Identity=22.0095693779904, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI70995211, Length=139, Percent_Identity=28.7769784172662, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787659, Length=246, Percent_Identity=31.3008130081301, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=28.9156626506024, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788597, Length=256, Percent_Identity=29.6875, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082183, Length=245, Percent_Identity=25.7142857142857, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=259, Percent_Identity=26.6409266409266, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=27.6018099547511, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=28.4046692607004, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=25.2, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=221, Percent_Identity=24.8868778280543, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=218, Percent_Identity=22.4770642201835, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=23.3644859813084, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19920382, Length=214, Percent_Identity=26.1682242990654, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=24.9027237354086, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=24.9027237354086, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653139, Length=269, Percent_Identity=27.5092936802974, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27679; Mature: 27548

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGGEVGVVWPQGGAQEGRGIVRVTLRHPGRLNAMSRAMWRQLREVFEGIQAREDARCVL
CCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
IEGEGDAFCAGGDISEYPAFRFDPGSLRDFHEHDVWGGLSAMLACDVPVVAVIRGACMGA
EECCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GMEIAACCDVRFAAVSARFGAPIARLGFPMAPREAALVAQAVGDGLARRMLLEAATFGAG
CCEEEHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLAAQGFLAAVVADDGLAAQAWASAERIAALAPEAARLNKQTLRACRQAAGGAPQAVSDP
CHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCCC
YAYAAGAEHREGISAFLEKRTPRF
HHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AGGEVGVVWPQGGAQEGRGIVRVTLRHPGRLNAMSRAMWRQLREVFEGIQAREDARCVL
CCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
IEGEGDAFCAGGDISEYPAFRFDPGSLRDFHEHDVWGGLSAMLACDVPVVAVIRGACMGA
EECCCCEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
GMEIAACCDVRFAAVSARFGAPIARLGFPMAPREAALVAQAVGDGLARRMLLEAATFGAG
CCEEEHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLAAQGFLAAVVADDGLAAQAWASAERIAALAPEAARLNKQTLRACRQAAGGAPQAVSDP
CHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCCCCCCCCCC
YAYAAGAEHREGISAFLEKRTPRF
HHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA