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The map label for this gene is dapF

Identifier: 120609493

GI number: 120609493

Start: 860960

End: 861838

Strand: Direct

Name: dapF

Synonym: Aave_0799

Alternate gene names: 120609493

Gene position: 860960-861838 (Clockwise)

Preceding gene: 120609491

Following gene: 120609494

Centisome position: 16.08

GC content: 72.81

Gene sequence:

>879_bases
ATGCAGATCCGCTTCACCAAAATGCAGGGCGCCGGCAACGATTTCGTCGTGCTCGACGAAACGCAGGGGCGCCTGGGCCT
CACGCCCGCGCAGTACCGCTACCTCGCCGACCGCCACTTCGGGGTGGGGGCCGACCAGATCCTCACCGTGCGTCCGTCGC
CGGCCGAGGGCATCGATTTCGAATACGTCATCCACAACGCCGACGGCGGCGAGGTCGAGCAGTGCGGCAACGGCGCGCGC
TGCTTCGCCCGCTACGTGCGCGACAAGGGCCTCACCGACCGCGACACCATCCGCGTGCAGACCCTGGCCGGCGTGATCGC
CCCGCGCCTGACGCCGGACGGCCGCGTCACCGTGGACATGGGCCGCCCGCGCTTCGACCCCGCGGAGGTGCCTTTCGACG
CCTCCGGCCTCGCACCCGCGGCCCGTGGGGCGGGGCAGACGTGGCCCCTGGCCCTCGGAGCCGGCGCCGATGCGCAGGTG
GTCGATGTGGCGGTGGTGTCCATGGGCAACCCGCATGCCGTGCAGCTGGTCGGCGACGTGGACGCGGCGCCGGTGGCGCA
CACCGGCCCGCTCATCGAGGGCCACGCGCGCTTCCCGCAGCGCGTGAACGCAGGCTACCTGCAGATCGAGGACCGGGCCC
GCGTGCGGCTGCGCGTGTATGAACGCGGTGCCGGCGAAACCCTGGCCTGCGGCACGGGCGCCTGCGCGGCCGTGGCCGCG
GGCATCCGCCTGGGCCTGCTGGACGCGCGCGTCGATGTCCGCACGCGCGGCGGCCTTCTCACCATCGCCTGGAGCGGCGA
CCCGTCCGAGTCCGTCTTCATGACCGGCCCGGCCACCACCGTCTTCGAAGGCCAGATCGACATTCCCGACGCACCATGA

Upstream 100 bases:

>100_bases
GGATGGGCGCAGGGGATGGAGGGCGCCATCATCGCGCGCCCGGCCGGCCCCTGCCGGCGAGGGGCCATGCCGCGCCTGCC
GGGCAACGATAATCCTGCGG

Downstream 100 bases:

>100_bases
CCCATCCGCATACCCCCGCCCAGGCCGTGCCTCCCATCACGGAAGAAGACATCGCCGAATTCCTCGCCAATACCCCGGGC
TTCTTCGAGCGCCATGCCGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MQIRFTKMQGAGNDFVVLDETQGRLGLTPAQYRYLADRHFGVGADQILTVRPSPAEGIDFEYVIHNADGGEVEQCGNGAR
CFARYVRDKGLTDRDTIRVQTLAGVIAPRLTPDGRVTVDMGRPRFDPAEVPFDASGLAPAARGAGQTWPLALGAGADAQV
VDVAVVSMGNPHAVQLVGDVDAAPVAHTGPLIEGHARFPQRVNAGYLQIEDRARVRLRVYERGAGETLACGTGACAAVAA
GIRLGLLDARVDVRTRGGLLTIAWSGDPSESVFMTGPATTVFEGQIDIPDAP

Sequences:

>Translated_292_residues
MQIRFTKMQGAGNDFVVLDETQGRLGLTPAQYRYLADRHFGVGADQILTVRPSPAEGIDFEYVIHNADGGEVEQCGNGAR
CFARYVRDKGLTDRDTIRVQTLAGVIAPRLTPDGRVTVDMGRPRFDPAEVPFDASGLAPAARGAGQTWPLALGAGADAQV
VDVAVVSMGNPHAVQLVGDVDAAPVAHTGPLIEGHARFPQRVNAGYLQIEDRARVRLRVYERGAGETLACGTGACAAVAA
GIRLGLLDARVDVRTRGGLLTIAWSGDPSESVFMTGPATTVFEGQIDIPDAP
>Mature_292_residues
MQIRFTKMQGAGNDFVVLDETQGRLGLTPAQYRYLADRHFGVGADQILTVRPSPAEGIDFEYVIHNADGGEVEQCGNGAR
CFARYVRDKGLTDRDTIRVQTLAGVIAPRLTPDGRVTVDMGRPRFDPAEVPFDASGLAPAARGAGQTWPLALGAGADAQV
VDVAVVSMGNPHAVQLVGDVDAAPVAHTGPLIEGHARFPQRVNAGYLQIEDRARVRLRVYERGAGETLACGTGACAAVAA
GIRLGLLDARVDVRTRGGLLTIAWSGDPSESVFMTGPATTVFEGQIDIPDAP

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=286, Percent_Identity=51.0489510489511, Blast_Score=274, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ACIAC (A1TKA9)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969171.1
- ProteinModelPortal:   A1TKA9
- SMR:   A1TKA9
- STRING:   A1TKA9
- GeneID:   4669737
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_0799
- NMPDR:   fig|397945.5.peg.691
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   A1TKA9
- ProtClustDB:   PRK00450
- BioCyc:   AAVE397945:AAVE_0799-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30871; Mature: 30871

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRFTKMQGAGNDFVVLDETQGRLGLTPAQYRYLADRHFGVGADQILTVRPSPAEGIDF
CEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHEEHHCCCCCCCCCEEEECCCCCCCCCE
EYVIHNADGGEVEQCGNGARCFARYVRDKGLTDRDTIRVQTLAGVIAPRLTPDGRVTVDM
EEEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCCCCEEEEEC
GRPRFDPAEVPFDASGLAPAARGAGQTWPLALGAGADAQVVDVAVVSMGNPHAVQLVGDV
CCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEEEECC
DAAPVAHTGPLIEGHARFPQRVNAGYLQIEDRARVRLRVYERGAGETLACGTGACAAVAA
CCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHH
GIRLGLLDARVDVRTRGGLLTIAWSGDPSESVFMTGPATTVFEGQIDIPDAP
HHEEEEEEEEEEEEECCCEEEEEECCCCCCCEEEECCCCEEEECEEECCCCC
>Mature Secondary Structure
MQIRFTKMQGAGNDFVVLDETQGRLGLTPAQYRYLADRHFGVGADQILTVRPSPAEGIDF
CEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHEEHHCCCCCCCCCEEEECCCCCCCCCE
EYVIHNADGGEVEQCGNGARCFARYVRDKGLTDRDTIRVQTLAGVIAPRLTPDGRVTVDM
EEEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHCCCCCCCEEEEEC
GRPRFDPAEVPFDASGLAPAARGAGQTWPLALGAGADAQVVDVAVVSMGNPHAVQLVGDV
CCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEEEEECC
DAAPVAHTGPLIEGHARFPQRVNAGYLQIEDRARVRLRVYERGAGETLACGTGACAAVAA
CCCCCCCCCCEEECCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHH
GIRLGLLDARVDVRTRGGLLTIAWSGDPSESVFMTGPATTVFEGQIDIPDAP
HHEEEEEEEEEEEEECCCEEEEEECCCCCCCEEEECCCCEEEECEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA