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The map label for this gene is ynfL [H]

Identifier: 120609418

GI number: 120609418

Start: 776423

End: 777367

Strand: Direct

Name: ynfL [H]

Synonym: Aave_0722

Alternate gene names: 120609418

Gene position: 776423-777367 (Clockwise)

Preceding gene: 120609417

Following gene: 120609419

Centisome position: 14.51

GC content: 61.27

Gene sequence:

>945_bases
ATGGAAATACGTCATCTGCGCTGCTTTCTAGCTGTTGCCGAAGAACTGCACTTCGCCCGCGCCGCCGAGCGGCTGCACAT
CGAGCAATCTCCGCTGTCGCGCACCATCAAGGAACTGGAAGAAGACCTGGGCGAACAGTTGTTCGTCCGCACCAGCCGTA
GCACGCGCCTGACACGGGCTGGCAAGCTGTTTCTGGAGCATGTGCCGCGCATCTTCACTGCCTTGCAGCAGGCACGCGAC
AGCGTGAAGGCGGCGGCCAACGGTTTCCACGGCCAATTGCGCATCGCCTTGTCCGAAGGCATCACACCCTCGCGCCTGTC
GTCCTTGCTGGCGCTTTGCCGACAAGAAGAACCCGAAGTCGAGATCCGTCTGTTCGAGGTCCCGCTGGCACAGCAGATCA
AGGGGCTGCATGATGACCTGTACGACGTGGGGTTTGCACAGTCCGATGAAGTGGGCGAAGGCATTGCAGCCCACGCGGTG
TGGAGTGATCCACTGATGGTGGCGGTGCCAGCGCGCCACCCGCTGCTCAAACACAAACGCATTCCCCTGGAAGAATTGAT
GCGTTACCCCCTCGTGCTGTGCGATCCGCTCGCGTGCGAAGGGCATGCGCGCCAGGTCGAGCGCGTGCTGCGCCGCTCGG
ACATGGAGCCGCTGATTGCCGAGCGCGTGGCCTCATGCGATTTGATGATGGCACTAGTGTCGGCGGGCTTTGCGCTGGGG
CTGACTGGCTCAGCACACTTAGCGGCTAGCCGCGAGTTGGGCGTAGTCGCACGGCCTTTGGAGGGTCGTTCCCCTGTGCT
GACGACCTACCTGCTACATCGAGAAGGTGAATCTTCGGAAGTGCTGTCTCGATTCATCGAAAGGGTGCACGCCATCGACT
TGCCAGAAGGAGCGAGGCTAGCACAACCACCTGAATCCAATCCTCCTGAGGACACCGAGCCATGA

Upstream 100 bases:

>100_bases
GATCGTAGCCGGCATATCACTACTCAAGCTCTCTTGAGTCAGGGTTGATGCCTTGCCACAAGGGCGCCCGGTCAGCGAAT
GAAGATGGAATGAGGGCTTC

Downstream 100 bases:

>100_bases
AGCGATCTATCCCTTTGTTACTGGTTGCTGCACTCGCGGCCTGCGGCCCGTCCGAGCCACCGAAGGAGCAAGCCAATGTG
CCTACCGTCGAAGAACTGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MEIRHLRCFLAVAEELHFARAAERLHIEQSPLSRTIKELEEDLGEQLFVRTSRSTRLTRAGKLFLEHVPRIFTALQQARD
SVKAAANGFHGQLRIALSEGITPSRLSSLLALCRQEEPEVEIRLFEVPLAQQIKGLHDDLYDVGFAQSDEVGEGIAAHAV
WSDPLMVAVPARHPLLKHKRIPLEELMRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
LTGSAHLAASRELGVVARPLEGRSPVLTTYLLHREGESSEVLSRFIERVHAIDLPEGARLAQPPESNPPEDTEP

Sequences:

>Translated_314_residues
MEIRHLRCFLAVAEELHFARAAERLHIEQSPLSRTIKELEEDLGEQLFVRTSRSTRLTRAGKLFLEHVPRIFTALQQARD
SVKAAANGFHGQLRIALSEGITPSRLSSLLALCRQEEPEVEIRLFEVPLAQQIKGLHDDLYDVGFAQSDEVGEGIAAHAV
WSDPLMVAVPARHPLLKHKRIPLEELMRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
LTGSAHLAASRELGVVARPLEGRSPVLTTYLLHREGESSEVLSRFIERVHAIDLPEGARLAQPPESNPPEDTEP
>Mature_314_residues
MEIRHLRCFLAVAEELHFARAAERLHIEQSPLSRTIKELEEDLGEQLFVRTSRSTRLTRAGKLFLEHVPRIFTALQQARD
SVKAAANGFHGQLRIALSEGITPSRLSSLLALCRQEEPEVEIRLFEVPLAQQIKGLHDDLYDVGFAQSDEVGEGIAAHAV
WSDPLMVAVPARHPLLKHKRIPLEELMRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
LTGSAHLAASRELGVVARPLEGRSPVLTTYLLHREGESSEVLSRFIERVHAIDLPEGARLAQPPESNPPEDTEP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=242, Percent_Identity=35.5371900826446, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=32.0754716981132, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=32.2033898305085, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI157672245, Length=234, Percent_Identity=30.7692307692308, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=29.3877551020408, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=25.7627118644068, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787601, Length=239, Percent_Identity=25.5230125523013, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=28.2051282051282, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787806, Length=301, Percent_Identity=24.9169435215947, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=240, Percent_Identity=24.1666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=127, Percent_Identity=34.6456692913386, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787949, Length=310, Percent_Identity=26.4516129032258, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786713, Length=243, Percent_Identity=31.2757201646091, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=32.8767123287671, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=26.7489711934156, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=34.9593495934959, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=190, Percent_Identity=31.0526315789474, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082132, Length=144, Percent_Identity=29.8611111111111, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34929; Mature: 34929

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRHLRCFLAVAEELHFARAAERLHIEQSPLSRTIKELEEDLGEQLFVRTSRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GKLFLEHVPRIFTALQQARDSVKAAANGFHGQLRIALSEGITPSRLSSLLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCE
EIRLFEVPLAQQIKGLHDDLYDVGFAQSDEVGEGIAAHAVWSDPLMVAVPARHPLLKHKR
EEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEECCCEEEEECCCCCHHHCCC
IPLEELMRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
CCHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEE
LTGSAHLAASRELGVVARPLEGRSPVLTTYLLHREGESSEVLSRFIERVHAIDLPEGARL
CCCCHHHHCCCCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
AQPPESNPPEDTEP
CCCCCCCCCCCCCC
>Mature Secondary Structure
MEIRHLRCFLAVAEELHFARAAERLHIEQSPLSRTIKELEEDLGEQLFVRTSRSTRLTRA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GKLFLEHVPRIFTALQQARDSVKAAANGFHGQLRIALSEGITPSRLSSLLALCRQEEPEV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCE
EIRLFEVPLAQQIKGLHDDLYDVGFAQSDEVGEGIAAHAVWSDPLMVAVPARHPLLKHKR
EEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHEEECCCEEEEECCCCCHHHCCC
IPLEELMRYPLVLCDPLACEGHARQVERVLRRSDMEPLIAERVASCDLMMALVSAGFALG
CCHHHHHHCCHHEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEE
LTGSAHLAASRELGVVARPLEGRSPVLTTYLLHREGESSEVLSRFIERVHAIDLPEGARL
CCCCHHHHCCCCCCCEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCC
AQPPESNPPEDTEP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]