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The map label for this gene is ltaA [H]

Identifier: 120609339

GI number: 120609339

Start: 688814

End: 689878

Strand: Reverse

Name: ltaA [H]

Synonym: Aave_0639

Alternate gene names: 120609339

Gene position: 689878-688814 (Counterclockwise)

Preceding gene: 120609342

Following gene: 120609337

Centisome position: 12.89

GC content: 71.27

Gene sequence:

>1065_bases
ATGCACGACTTCCGCAGCGATACCGTCACGCAGCCCACCGCCGCCATGCGCGAAGCCATGCTGACAGCCCCCCTGGGCGA
CGACGTCTTCGGCGACGACCCTTCCGTGAACGACCTGCAGAACCATGCCGCGGAACTGCTGGGGTTCGACGCCGCGCTGT
TCGCCCCCAGCGGCACGCAGGCCAACCTCATCGCGCTCATGGGCCACTGCCAGCGCGGCGACGAGGCCATCGTGGGCCAG
AGCTGGCACACCTACCGCTGGGAAGGCGGCGGCATGGCGACGCTGGGCTCCATCCATCCCCAGCCCATCGAGAACCGCGC
CGACGGCACGTTGTGCCTGCGCGACATCGCCGCGGCCATCAAGCCGGACGACCCGCACTTCGCCCGCACCCGCCTGGTGG
TGCTGGAAAACACCGCTGGCGGGCAGGTCCTGCCCAACCCCTACATCGCCGACGTGGCCGCCCTCGCGCGCAGCCGCCAG
CTGGCCATGCACCTCGACGGCGCCCGCCTCTTCAATGCCGCGACCGCCAACGCCATGGCCCACGGCACGGACGTGTACGA
CGAGGCGCGCGCCATCTGCGCGCATTTCGACACCGCCTCGATCTGCCTGAGCAAGGGCCTGGGCGCTCCCGTGGGGTCCC
TGCTGCTCGGCTCCCACGACTTCATCGCGCATGCCCGCCGCACCCGCAAGATGCTGGGCGGCGCCATGCGGCAGATCGGC
ATGCTCGCGGCCGCCGGGCGCTATGCCCTCGACCACCACGTGCGCCGCATGGCGGACGACCACGCCCTGCTTCGGTCGCT
GGCCGAAGGCCTGGCGGAGGTCGGCCGCAGCCATCCGGTGCTGCGCCGGCGCCTCAGCGTGGCCTCCGCGCACACCAACA
TCATGTTCACCGACGTGCACTCGGACATCGCCCCCGCCCTGATGGCCTGGCTGTCCCAGCACGGCATCTACGTCACGCGC
AGCCTCTGCGGCGGGCAGATCCGCCTGCGCTGGGTCACCCACCTGGACGTGGGGGCCGAGGACGTCGAGCACACCCTGGG
CTGCGTGGAGGAATTCCGGGGCTGA

Upstream 100 bases:

>100_bases
GGCATCCCGGGGGATTTGCGCCGCTTCGCTCCCGGGGCAGGTAGTCGCAGCTACCATCTCTGCCGAGACCGCCCCCATCT
CCCCCCAGGAGCCCGATCCG

Downstream 100 bases:

>100_bases
GGCCCGCTCCCCGGGGCCGCGCCCCCGGGTTTTCAGCGCGCCGGATAGCTGCGCGCGCCGAAAATCCCGCTGCCCACGCG
CACCATGGTGCTGCCCGCGT

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MHDFRSDTVTQPTAAMREAMLTAPLGDDVFGDDPSVNDLQNHAAELLGFDAALFAPSGTQANLIALMGHCQRGDEAIVGQ
SWHTYRWEGGGMATLGSIHPQPIENRADGTLCLRDIAAAIKPDDPHFARTRLVVLENTAGGQVLPNPYIADVAALARSRQ
LAMHLDGARLFNAATANAMAHGTDVYDEARAICAHFDTASICLSKGLGAPVGSLLLGSHDFIAHARRTRKMLGGAMRQIG
MLAAAGRYALDHHVRRMADDHALLRSLAEGLAEVGRSHPVLRRRLSVASAHTNIMFTDVHSDIAPALMAWLSQHGIYVTR
SLCGGQIRLRWVTHLDVGAEDVEHTLGCVEEFRG

Sequences:

>Translated_354_residues
MHDFRSDTVTQPTAAMREAMLTAPLGDDVFGDDPSVNDLQNHAAELLGFDAALFAPSGTQANLIALMGHCQRGDEAIVGQ
SWHTYRWEGGGMATLGSIHPQPIENRADGTLCLRDIAAAIKPDDPHFARTRLVVLENTAGGQVLPNPYIADVAALARSRQ
LAMHLDGARLFNAATANAMAHGTDVYDEARAICAHFDTASICLSKGLGAPVGSLLLGSHDFIAHARRTRKMLGGAMRQIG
MLAAAGRYALDHHVRRMADDHALLRSLAEGLAEVGRSHPVLRRRLSVASAHTNIMFTDVHSDIAPALMAWLSQHGIYVTR
SLCGGQIRLRWVTHLDVGAEDVEHTLGCVEEFRG
>Mature_354_residues
MHDFRSDTVTQPTAAMREAMLTAPLGDDVFGDDPSVNDLQNHAAELLGFDAALFAPSGTQANLIALMGHCQRGDEAIVGQ
SWHTYRWEGGGMATLGSIHPQPIENRADGTLCLRDIAAAIKPDDPHFARTRLVVLENTAGGQVLPNPYIADVAALARSRQ
LAMHLDGARLFNAATANAMAHGTDVYDEARAICAHFDTASICLSKGLGAPVGSLLLGSHDFIAHARRTRKMLGGAMRQIG
MLAAAGRYALDHHVRRMADDHALLRSLAEGLAEVGRSHPVLRRRLSVASAHTNIMFTDVHSDIAPALMAWLSQHGIYVTR
SLCGGQIRLRWVTHLDVGAEDVEHTLGCVEEFRG

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=342, Percent_Identity=51.1695906432749, Blast_Score=323, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI193206522, Length=355, Percent_Identity=38.8732394366197, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI193206520, Length=355, Percent_Identity=38.8732394366197, Blast_Score=221, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI193206524, Length=355, Percent_Identity=38.8732394366197, Blast_Score=221, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6320789, Length=334, Percent_Identity=34.7305389221557, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355439, Length=308, Percent_Identity=43.8311688311688, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 38149; Mature: 38149

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDFRSDTVTQPTAAMREAMLTAPLGDDVFGDDPSVNDLQNHAAELLGFDAALFAPSGTQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEECCCCCC
ANLIALMGHCQRGDEAIVGQSWHTYRWEGGGMATLGSIHPQPIENRADGTLCLRDIAAAI
HHHHHHHHHCCCCCCEEECCCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHC
KPDDPHFARTRLVVLENTAGGQVLPNPYIADVAALARSRQLAMHLDGARLFNAATANAMA
CCCCCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHH
HGTDVYDEARAICAHFDTASICLSKGLGAPVGSLLLGSHDFIAHARRTRKMLGGAMRQIG
CCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MLAAAGRYALDHHVRRMADDHALLRSLAEGLAEVGRSHPVLRRRLSVASAHTNIMFTDVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECH
SDIAPALMAWLSQHGIYVTRSLCGGQIRLRWVTHLDVGAEDVEHTLGCVEEFRG
HHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHDFRSDTVTQPTAAMREAMLTAPLGDDVFGDDPSVNDLQNHAAELLGFDAALFAPSGTQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHEECCCCCC
ANLIALMGHCQRGDEAIVGQSWHTYRWEGGGMATLGSIHPQPIENRADGTLCLRDIAAAI
HHHHHHHHHCCCCCCEEECCCCCEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHC
KPDDPHFARTRLVVLENTAGGQVLPNPYIADVAALARSRQLAMHLDGARLFNAATANAMA
CCCCCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHH
HGTDVYDEARAICAHFDTASICLSKGLGAPVGSLLLGSHDFIAHARRTRKMLGGAMRQIG
CCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MLAAAGRYALDHHVRRMADDHALLRSLAEGLAEVGRSHPVLRRRLSVASAHTNIMFTDVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECH
SDIAPALMAWLSQHGIYVTRSLCGGQIRLRWVTHLDVGAEDVEHTLGCVEEFRG
HHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 0.22 {L-allo-Thr}} 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]